Detection and clinical significance of circulating tumor cells in gastric cancer / 中华胃肠外科杂志

The death of patients with gastric cancer is mainly due to its recurrence and metastasis, and circulating tumor cell (CTC) is the necessary condition of metastasis. As liquid biopsy, CTC detection has its certain clinical significance. The detection is required after enrichment because circulating tumor cells are rare. Many enrichment methods have been developed: methods based on physical characteristics of TCT, like density, size and dielectric properties and so on; immunogenicity, like Cell Search System; and microfluidic chip technology. The immunofluorescence is commonly used to identify CTC in gastric cancer and the isolated CTC can also be used for the following analysis on the level of nucleic acid, protein and gene regulation. Detection of CTC in gastric cancer is helpful to judge the prognosis, assess staging, monitor the curative effect and guide the development of drug. There are many challenges for clinical transformation of CTC: the lower enrichment efficiency, the less specific surface markers, the uncertain diagnostic efficiency and so on, but it also has the good research prospect because it is non-invasive, repeatable and can real-time monitor the condition and guide the clinical treatment compared with pathological biopsy. In this paper, the detection and identification methods, and clinical value of CTC in gastric cancer patients are reviewed.

Application of biochip technology and its application on medical diagnosis / 中国医疗器械杂志

Biochip analytical technology shows high throughput property for multi-samples measurement, so can reduce the required amount of samples and time used for determination. The technology quickly developed in recent years and has been applied in medical diagnosis and other analytical areas including gene chip, protein chip, lab-on-a-chip, tissue microarray, cell microarray, carbohydrate microarray and so on. This paper overviewed the current development of biochip technology, and explored the perspective of its application.

Expressions of TNF-alpha, IL-6, CRP, and MCP-1 in phlegm-damp constitution population detected by multiplexed Luminex assay / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To study the expression changes of tumor necrosis factor-alpha (TNF-(alpha), interleukin-6 (IL-6), C-reactive protein (CRP), monocyte chemotactic protein 1 (MCP-1), and their correlation with obesity in 20 -50 years old population of phlegm-damp constitution (PDC) and of normal constitution (NC) using Luminex technique.</p><p><b>METHODS</b>Totally 101 population were recruited from Health Examination Center of Puren Hospital from April to December 2011. Based on body mass index (BMI), the subjects were assigned to four groups, i.e., the obesity of PDC group (Group OBT, BMI > or = 24 kg/m2, 30 cases), the non-obesity of PDC group (Group NOBT, BMI < 24 kg/m2, 25 cases), the obesity of non-PDC group (Group OBNT, BMI > or = 24 kg/m2, 28 cases), the NC group (Group P, BMI < 24 kg/m2, 18 cases). The BMI and body fat percent (FAT%) were compared among the 4 groups. Serum levels of TNF-alpha, IL-6, CRP, and MCP-1 were measured with Luminex technique.</p><p><b>RESULTS</b>BMI was significantly higher in Group OBT and Group OBNT than in Group NOBT and Group P (all P < 0.05). The FAT% was significantly higher in Group OBT and Group OBNT than in Group P (P < 0.01). The serum TNF-alpha level in Group OBT was higher than in Group P (P < 0.01). The serum CRP and MCP-1 levels were significantly higher in Group OBT, NOBT, and OBNT than in Group P (P < 0.05, P < 0.01). The score for PDC was positively correlated with TNF-alpha, IL-6, and MCP-1 levels (P < 0.05).</p><p><b>CONCLUSIONS</b>Abnormal higher levels of inflammatory factors exist in 20 -50 years old population of PDC. Chronic inflammation exists in population of PDC and obesity people.</p>

Screening of differentially expressed microRNAs in bone marrow-derived mesenchymal stem cells during osteoporosis and their function in stem cell differentiation / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To screen differentially expressed microRNAs in bone marrow-derived mesenchymal stem cells (BMMSCs) during osteoporosis and their function in stem cell differentiation.</p><p><b>METHODS</b>The osteoporosis model was induced by ovariectomy in mice.The differentially expressed microRNAs in BMMSCs during osteoporosis were screened with microRNA microarray chip and their expressions during osteogenesis and adipogenesis were detected by RT-PCR.</p><p><b>RESULTS</b>Ten differentially expressed microRNAs were detected in BMMSC during osteoporosis. The expression of 5 specific microRNAs altered significantly during osteogenic differentiation and 3 microRNAs altered during adipogenic differentiation.</p><p><b>CONCLUSION</b>A serials of differentially expressed microRNAs may be involved in functional defects of BMMSC during osteoporosis.</p>

Applicability of the multiplex quantitative antibody array system for early diagnosis of hepatocellular carcinoma / 中华肝脏病杂志

<p><b>OBJECTIVE</b>To develop an early and accurate detection method for hepatocellular carcinoma (HCC) based on detection of tumor-associated serum markers using a multiplex quantitative antibody array.</p><p><b>METHODS</b>The double-antibody sandwich principle was used to establish an antibody array composed of eight cancer-related serum markers, including alpha-fetoprotein (AFP), hepatocyte growth factor (HGF), insulin-like growth factor (IGF), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10), transforming growth factor-beta 1 (TGF-b1), and vascular endothelial growth factor (VEGF). Serum samples from 160 cases of clinically diagnosed HCC and from 58 cases of liver cirrhosis (LC; controls) were obtained to test the array. Sixty percent of the samples were randomly selected for use as the training set (HCC, n = 96; LC, n = 36), and the remaining 40% was used as the test set (HCC, n = 64; LC, n = 22). The SPSS statistical software was used to perform logistic regression analysis and to create a diagnostic model.</p><p><b>RESULTS</b>When used with the training set, the model had sensitivity of 93.3%, specificity of 83.3%, and accuracy of 90.9%. When used with the test set, the model had sensitivity of 89.0%, specificity of 77.3%, and accuracy of 86.0%. The traditional serum AFP value (cut-off value of 20 ng/mL) showed 70.0% diagnostic sensitivity, 59.0% specificity, and 64.0% accuracy.</p><p><b>CONCLUSION</b>The newly developed multiplex quantitative antibody detection system has high sensitivity and specificity. The diagnostic model with AFP and seven other cancer-related factors was superior to the traditional AFP only approach for early diagnosis of liver cancer, indicating its potential clinical value.</p>

Progress of study on the detection technique of microRNA / 中国实验血液学杂志

MicroRNAs (miRNAs) are small noncoding RNA molecules that negatively regulate gene expression via degradation or translational repression of their targeted mRNAs. MiRNAs are involved in critical biologic processes, including development, cell differentiation, proliferation and the pathogenesis of disease. This review focuses on recent researches on the detection techniques of miRNA including micorarray technique, Northern blot, real-time quantitative PCR, detection technique of miRNA function and so on.

Development of a print quality inspection system for biochips / 中国医疗器械杂志

An automatic inspection system for biochip's print quality is presented in this paper. It consists of an automatic mechanical control, a CCD sensor for getting the image of PET boart, and the special computer software for image processing and recognition. Experimental results indicate that this system is capable of providing a precise and effective realtime inspection for biochips' print quality.

Research of the expression of PTEN in squamous cell carcinoma of larynx by the tissue chip technology / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To evaluate the expression of PTEN in squamous cell carcinoma of larynx and its relationship with factors like pathologic fractionation, clinical TNM stage, and prognosis by the tissue chip technology.@*METHOD@#We studied the expression of PTEN gene and its mRNA on a series of 146 cases of primary laryngeal carcinoma patients, 40 cases of precancerous lesion and 26 cases of vocal fold polyp by tissue chip by, immunohistochemistry and in situ hybridization method. The observed data observed and some relevant clinical dada were statistically analyzed.@*RESULT@#The expression of PTEN in vocal fold polyp was negative, and its positive expression in precancerous lesion and laryngeal carcinoma were 40% and 43.15% respectively by immunohistochemistry, and were 72.50 and 59.59% respectively in situ hybridization. The difference between the expression of PTEN in laryngeal carcinoma and its pathological fraction and prognosis was statistically significant, but was not significant between that and location, clinical stage and LN metastasis. The mRNA expression of PTEN was higher than that of the protein expression in precancerous lesion and cancer tissue.@*CONCLUSION@#The tissue microarray technique required shorter time and less expense, and showed higher consistency in our essays. And the present study suggests PTEN was a prognosis factor of the Laryngeal carcinoma.

BRG1 expression in prostate carcinoma by application of tissue microarray / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the expression of gene BRG1 in prostatic intraepithelial neoplasia and adenocarcinoma, and the relationship between gene BRG1 expression and the clinicopathological features of prostate carcinoma.</p><p><b>METHODS</b>Gene BRG1 expression was evaluated in 37 cases of human prostate carcinoma, 13 human prostatic intraepithelial neoplasia (PIN) and 14 human benign prostatic hyperplasia (BPH) by using immunohistochemistry (EnVision method) and tissue microarray.</p><p><b>RESULTS</b>The positive rates of BRG1 protein were 81.08% (30/37), 38.46% (5/13) and 14.28% (2/14) in prostate carcinoma, PIN and BPH, respectively, significantly higher in the first group than in the latter two (P < 0.05). There was no statistically significant difference in BRG1 gene expression either between PIN and BPH (P > 0.05) or between the groups of the moderate differentiation (the Gleason histologic grading: 5-7) and the lower one (the Gleason histologic grading: 8-10) (P > 0.05).</p><p><b>CONCLUSION</b>BRG1 may play an important role in the development of prostate carcinoma. Tissue microarray technology, with the advantages of high throughput, conciseness, rapidity, high efficiency, low cost, and nice reproducibility, has significant practical value and broad application prospects in pathology.</p>

The advance of research in electro-physiology and chip techniques for olfaction and gustation / 生物医学工程学杂志

This paper introduces the new research achievement and progress of electro-physiology in olfaction and gustation. Classical implements such as patch-clamp or glass pipette are not appropriate in the dynamic detection of cellular signal transportation. In view of this, we have analyzed the feasibilities and challenges of olfactory or gustatory cell-based biosensors such as field effect transistor (FET) and light addressable potentiometric sensor (LAPS). Finally we present the research work carried our in out lab and a future prospective on the development in this field.

Biochip micromachining technology adopting laser technology / 生物医学工程学杂志

This paper deals with the manufacturing state of the art of biochip, and introduces a new method--laser microtechnology, including its developing procedure, characteristics and function in biochip production.