ABSTRACT
Campylobacteriosis is an extremely important zoonosis, circulating freely in the environment. In nonhuman primates kept in open facilities and bred for experimental purposes, the presence of Campylobacter spp. could cause severe damage to the production and interfere with the results of scientific research. In this paper, we assessed the circulation of Campylobacter spp. in a colony of clinically healthy rhesus monkeys (Macaca mulatta) destined to research. The analysis was carried out during seven non-consecutive years. Data showed that despite several changes made in animal management along the studied years in order to control this zoonosis, reduction of bacterial charge did not occur. Significant differences among the age groups and sex were observed. Infants showed higher susceptibility than adult animals. In general males were more infected than females. Modifications adopted in the handling techniques need to be reviewed with the intent of improving the production, reducing bacterial infection of the stock and avoiding undesirable cross reactions in the research carried out with these animals. Therefore, this paper alerts professionals that work directly with captive rhesus monkeys about the risks of Campylobacter spp. infection and possible interference on the experimental procedures.
Subject(s)
Animals , Male , Female , Animals, Laboratory/microbiology , Campylobacter Infections/veterinary , Campylobacter/isolation & purification , Macaca mulatta/microbiology , Monkey Diseases/microbiology , Campylobacter Infections/diagnosis , Campylobacter/classification , Campylobacter/drug effects , Longitudinal Studies , Microbial Sensitivity Tests , Monkey Diseases/diagnosisABSTRACT
The study describes a comparison between molecular hybridization using a non-radiolabeled, thymine-thymine (TT) dimerized synthetic oligonucleotide complementary DNA probe and electron microscopy for the detection of hepatitis E virus genome in bile. Spot hybridization with the TT dimerized probe was found to be more sensitive and specific compared to electron microscopy.
Subject(s)
Animals , Bile/microbiology , DNA, Complementary , DNA, Viral , Genome, Viral , Hepatitis E/microbiology , Hepatitis E virus/genetics , Microscopy, Electron/methods , Monkey Diseases/microbiology , Nucleic Acid Hybridization/methods , Oligonucleotide Probes , Pyrimidine Dimers , Sensitivity and Specificity , Time FactorsABSTRACT
O objetivo deste trabalho foi estudar alguns aspectos da epidemiologia desta micose, pesquisando a ocorrência de paracoccidioidomicose-infecçäo em animais da Ordem Primata, uma vez que säo filogeneticamente os mais próximos ao homem, único hospedeiro naturalmente susceptível a esta micose sistêmica, no atual estágio de conhecimento. Foram realizados testes de hipersensibilidade do tipo tardio com paracoccidioidina em 33 exemplares de Cebus apella (macaco prego), obtendo-se 33,33//de positividade. Foram também executadas biópsias de reaçöes intradérmicas para exame histológico, testes sorológicos, de fixaçäo de complemento e precipitaçäo em meio líquido no soro destes animais. Os resultados obtidos permitiram verificar a ocorrência de paracoccidioidomicose-infecçäo em primatas näo humanos, sugerindo a possível participaçäo destes animais na epidemiologia da paracoccidioidomicose