Alcanivorax dieselolei, an alkane-degrading bacterium associated with the mucus of the zoanthid Palythoa caribaeorum (Cnidaria, Anthozoa) / Alcanivorax dieselolei, uma bactéria degradadora de alcanos associada ao muco do zoantídeo Palythoa caribaeorum (Cnidaria, Anthozoa)

Analyses of 16S rDNA genes were used to identify the microbiota isolated from the mucus of the zoanthid Palythoa caribaeorum at Porto de Galinhas on the coast of Pernambuco State, Brazil. This study is important as the first report of this association, because of the potential biotechnological applications of the bacterium Alcanivorax dieselolei, and as evidence for the presence of a hydrocarbon degrading bacterium in a reef ecosystem such as Porto de Galinhas.

Análises dos genes 16S rDNA foram empregadas para identificar a microbiota isolada do muco do zoantídeo Palythoa caribaeorum de Porto de Galinhas, litoral do estado de Pernambuco, Brasil. Este estudo é importante pelo ineditismo dessa associação, pelas relevantes aplicações biotecnológicas da bactéria Alcanivorax dieselolei e pela indicação da presença de uma bactéria degradadora de hidrocarbonetos em um ecossistema recifal como o de Porto de Galinhas.

Alcanivorax dieselolei, an alkane-degrading bacterium associated with the mucus of the zoanthid Palythoa caribaeorum (Cnidaria, Anthozoa) / Alcanivorax dieselolei, uma bactéria degradadora de alcanos associada ao muco do zoantídeo Palythoa caribaeorum (Cnidaria, Anthozoa)

Analyses of 16S rDNA genes were used to identify the microbiota isolated from the mucus of the zoanthid Palythoa caribaeorum at Porto de Galinhas on the coast of Pernambuco State, Brazil. This study is important as the first report of this association, because of the potential biotechnological applications of the bacterium Alcanivorax dieselolei, and as evidence for the presence of a hydrocarbon degrading bacterium in a reef ecosystem such as Porto de Galinhas.

Análises dos genes 16S rDNA foram empregadas para identificar a microbiota isolada do muco do zoantídeo Palythoa caribaeorum de Porto de Galinhas, litoral do estado de Pernambuco, Brasil. Este estudo é importante pelo ineditismo dessa associação, pelas relevantes aplicações biotecnológicas da bactéria Alcanivorax dieselolei e pela indicação da presença de uma bactéria degradadora de hidrocarbonetos em um ecossistema recifal como o de Porto de Galinhas.

Broncofibroscopia para el manejo de la atelectasia y los estudios microbiológicos en pacientes ventilados / Bronchoscopy for the atelectasis management and microbiological studies in ventilated patients

La atelectasia es una complicación frecuente en pacientes bajo ventilación mecánica artificial que aumenta la estadía, los costos y la mortalidad de los pacientes en la unidad de cuidados intensivos, representa un problema sin resolver para el paciente ventilado, tiene efecto deletéreo sobre algunos parámetros ventilatorios como la diferencia álveolo-arterial de oxígeno, la relación entre presión de oxígeno y fracción inspirada de oxígeno y la compliancia pulmonar, la broncofibroscopia es efectiva para prevenirla, diagnosticarla y tratarla en este grupo de enfermos. Obtener muestras de secreciones respiratorias para estudios microbiológicos en pacientes ventilados ha sido un tema muy debatido en la literatura médica, disminuir la frecuencia de neumonía asociada a la ventilación mecánica y aislar el germen responsable es muy útil para la elección del antimicrobiano, la broncofibroscopia demuestra ser superior a otras técnicas incluida la aspiración con sondas a ciegas en los pacientes ventilados

Atelectasis is a frequent complication in patients on artificial ventilation which increases the stay, cost and mortality of patients in the intensive care unit is an unsolved problem for the ventilated patient, has deleterious effect on some ventilatory parameters as alveolar-arterial oxygen difference, the relationship between oxygen pressure and fraction of inspired oxygen and lung compliance, bronchoscopy is effective to prevent, diagnose it and treat it in this group of patients. Get samples of respiratory secretions for microbiologic studies in ventilated patients has been a very debated topic in the literature, reducing pneumonia frequency associated with mechanical ventilation and isolate the responsible germ is very useful for the choice of antimicrobial, the bronchoscopy shows be superior to other techniques including aspiration probe blindly in ventilated patients

Evaluation of three Mycobacterium leprae monoclonal antibodies in mucus and lymph samples from Ziehl-Neelson stain negative leprosy patients and their household contacts in an indian community

Mucus and lymph smears collected from leprosy patients (9) and their household contacts (44) in the Caño Mochuelo Indian Reservation, Casanare, Colombia, were examined with monoclonal antibodies (MoAb) against Mycobacterium leprae. The individuals studied were: 5 borderline leprosy (BB) patients, 4 with a lepromatus leprosy (LL), all of whom were undergoing epidemiological surveillance after treatment and 44 household contacts: 21 of the LL and 23 contacts of the BB patients. The MoAb were reactive with the following M. leprae antigens: 65 kd heat shock protein, A6; soluble antigen G7 and complete antigen, E11. All the samples were tested each of the MoAb using the avidin-biotin-peroxidase technique and 3,3 diaminobenzidine as chromogen. The patients and household contacts studied were all recorded as Ziehl-Neelson stain negative. The MoAb which showed optimal reaction was G7, this MoAb permited good visualization of the bacilli. Five patients with BB diagnosis and one with LL were positive for G7; of the BB patients' household contacts, 9 were positive for G7; 7 of the LL patients' household contacts were positive for the same MoAb. MoAb G7 allowed the detection of bacillar Mycobacterium spp. compatible structures in both patients and household contacts. G7 permited the visualization of the complete bacillus and could be used for early diagnosis and follow-up of the disease in patients.

criptococosis: estudio de catorce casos con diagnostico comprobado por el laboratorio / Cryptococcosis: study of 14 cases with confirmed laboratory diagnosis

En un periodo de 4 anos se diagnosticaron, por procedimientos de laboratorio 14 casos de meningitis por Crytococcus neoformans y fue posible realizar el seguimiento serologico en 4 de ellos. La positividad de las pruebas cuando se realizaron el LCR fue de 75% para el examen directo, de 67% para el cultivo y de 84.6% para las dos pruebas en conjunto. Cuando se realizo la prueba de aglutinacion del latex para determinacion del antigeno en suero. Mediante inmunofluorescencia indirecta logramos demostrar anticuerpos en el 43% de los pacientes. El seguimiento clinico y serologico en tres pacientes y serologico en una permitio corroborar el valor pronostico de la prueba de aglutinacion del latex