ABSTRACT
The aim of this study was to determine IS6110 banding pattern of Mycobacterium tuberculosis [MTB] isolates for evaluation of tuberculosis [TB] transmission. These isolates were obtained from intermediate laboratories of six major provinces of Iran; East Azarbaijan, West Azarbaijan, Khorasan, Kerman, Kermanshah and Fars. Restriction fragment length polymorphism [RFLP] was performed on 100 suitable isolates, which have been obtained from some laboratories thought Iran. Fingerprinting was done using the oligonucleotide 6110 a' [5
Subject(s)
Mycobacterium tuberculosis/genetics , Polymorphism, Restriction Fragment Length , Oligonucleotides/biosynthesis , Hybridization, Genetic , Polymerase Chain Reaction , DNA ProbesABSTRACT
Trypanosoma cruzi, causador da tripanossomíase americana, é um microrganismo exposto a uma variedade de espécies reativas de oxigênio, geradas como conseqüência do metabolismo de drogas tripanocidas, ou pelo hospedeiro como resposta fisiológica à invasão, além daqueles produzidos internamente em conseqüência dos processos oxidativos do parasita. Em qualquer circunstância, as células precisam proteger-se contra os produtos da redução parcial do oxigênio. Os tripanossomatídeos são aparentemente deficientes na defesa antioxidante. Embora possuindo uma superóxido dismutase, carecem de glutationa peroxidase e catalase, que são necessárias para a remoção do peróxido de hidrogênio. Além disso, glutationa, o maior composto sulfidrílico antioxidante em células de mamíferos, está presente em baixas concentrações nos tripanossomatídeos...
Subject(s)
Antibodies, Protozoan , Cloning, Molecular , Crithidia fasciculata , Glutathione Peroxidase/deficiency , Oligonucleotides/biosynthesis , Superoxide Dismutase , Trypanosoma cruzi/immunology , Blotting, Western , Cell Culture Techniques , Electrophoresis, Polyacrylamide Gel , Fluorescent Antibody TechniqueABSTRACT
Using synthetic peptides and a combinatorial library of 56 mer random oligonucleotides, we have developed reagents that behave as "synthetic antibodies". The results obtained with the protein phosphatase 2A as a model system are shown here. The specificity of these reagents, named "oligobodies", has been demonstrated by Western blot analysis and immunohistochemistry. The oligobodies have enormous advantages compared to antibodies: their production is independent of the immune system, they can be prepared in a few days and there is no need for a purified target protein. These reagents can be produced even if the corresponding protein was never isolated or purified, since only a partial DNA suquence from a database provides enough information to make them.