ABSTRACT
The objective of this study was to elucidate the cause of the spermatogenic defect in idiopathic azoospermia and non-mosaic type of Klinefelter syndrome. Genomic DNAs from 9 cases of Korean idiopathic azoospermia and 6 of Korean non-mosaic type of Klinefelter syndrome were used for the detection of Y chromosome microdeletions by polymerase chain reaction using 60 primers. Microdeletions of the Y chromosome were found in 1 of 9 (11.1%) patients with idiopathic azoospermia, whereas none was deleted in non-mosaic type of Klinefelter syndrome. This result suggests that Y chromosome microdeletions could be one of the etiologic factors in idiopathic azoospermia.
Subject(s)
Humans , Male , Gene Dosage , Klinefelter Syndrome/classification , Oligospermia/classification , Polymerase Chain Reaction , Sequence Deletion , Sequence Tagged Sites , Spermatogenesis , X Chromosome/genetics , Y Chromosome/geneticsABSTRACT
Variations in semen analyses of 177 males over a 1 year period were assessed. The average means of total counts, motility, morphology, total motile count and non-motile % were determined for 5 classes of patients ranging from azoospermic to normospermic. Positive relationships between a falling sperm count, a decrease in motility and total motile counts were seen. Also, increasingly, abnormal forms were found with lower sperm counts.
Subject(s)
Adolescent , Adult , Humans , Male , Middle Aged , Oligospermia/classification , Reproducibility of Results , Severity of Illness Index , Sperm Count/methods , Sperm Motility/physiology , Spermatozoa/abnormalitiesABSTRACT
Se estudiaron 85 pacientes subfértiles en la clínica de Andrología del Hospital General del Centro Médico Nacional. La azoospermia fue la alteración más frecuente en grupo, y los factores etiológicos que propiciaron con mayor frecuencia esterilidad fueron infecciones, varicoceles y disgenesias gonadles. El estudio integral de estos pacientes desde los puntos de vista clínico, de laboratorio y de gabinete permitió hacer un diagnóstico más preciso y tener un pronóstico definitivo en cuanto a fertilidad