ABSTRACT
Background : Fatty acid synthase (FASN) is overexpressed in a variety of human cancers, and may be involved in cancer metastasis. Hence, the strategies targeted on FASN may have therapeutic potential for treating cancer metastasis. Objectives : The aim of this study is to investigate the correlation of FASN expression with metastasis in human osteosarcoma. Materials and Methods : Human osteosarcoma cell lines U2-OS and osteosarcoma biopsy specimens were employed in this study. The expression of FASN protein in osteosarcoma specimens was detected by IHC (immunohistochemistry) and the relationship with metastasis was analyzed. We performed the cerulenin, an inhibitor of FASN, to inhibit FASN expression in U2-OS cells. Western blot and RT-PCR were performed to investigate the expression of FASN in U2-OS cells. Cells mobility was detected by wound healing and Transwell assays. Results : Results showed that the FASN expression level in the cases with pulmonary metastases was significantly higher than in those without metastasis. In vitro, the invasion and migration of U2-OS cells were suppressed by inhibiting FASN. Our findings suggested that FASN may be involved in osteosarcoma metastasis
Subject(s)
Adolescent , Antineoplastic Agents/pharmacology , Blotting, Western , Cell Movement/drug effects , Cell Proliferation/drug effects , Cells, Cultured , Cerulenin/pharmacology , Child , Child, Preschool , Enzyme Inhibitors/pharmacology , Fatty Acid Synthase, Type I/antagonists & inhibitors , Female , Gene Expression Profiling , Humans , Immunohistochemistry , Male , Osteosarcoma/pathology , Osteosarcoma/secondary , Real-Time Polymerase Chain ReactionABSTRACT
Background: Metastasis of soft tissue sarcoma most commonly occurs to the lungs. There are very few studies on histology of pulmonary metastatectomy and hardly any wherein the histology of the primary tumor has been compared with the metastasis. Aims and Objectives: To review histologically all metastatic sarcomas to lung and compare with the primary where available. Materials and Methods: Ninety-five patients with pulmonary metastases from sarcoma were analyzed histologically for type of sarcoma, chemotherapy-related changes, and changes in adjacent lung. Various clinical parameters like laterality, multiplicity, and interval between primary and metastasis were also studied. Results: Osteosarcoma constituted half of the metastatic sarcomas (48 cases, 50.5%) followed by synovial sarcoma (16 cases, 16.8%) and high grade spindle cell sarcoma-NOS (10 cases, 10.5%). The histology of primary and the metastases was similar in 60% of cases of osteosarcoma. Conclusions: Osteosarcoma is the commonest metastatic sarcoma to the lung. There is often a change to fibroblastic histology in patients of conventional osteosarcoma treated with chemotherapy.
Subject(s)
Adult , Bone Neoplasms/pathology , Child , Female , Humans , Lung Neoplasms/secondary , Male , Middle Aged , Neoplasm Staging , Osteosarcoma/secondary , Sarcoma/secondary , Sarcoma, Synovial/secondary , Young AdultSubject(s)
Adult , Combined Modality Therapy , Humans , Male , Ophthalmologic Surgical Procedures , Orbital Neoplasms/secondary , Orbital Neoplasms/therapy , Osteosarcoma/secondary , Osteosarcoma/therapy , Radiotherapy , Sarcoma/pathology , Sarcoma/therapy , Sphenoid Bone/pathology , Thoracic Wall/pathologySubject(s)
Adolescent , Bone Neoplasms/pathology , Bone Neoplasms/diagnostic imaging , Diagnosis, Differential , Femur , Humans , Lung Neoplasms/diagnostic imaging , Lung Neoplasms/secondary , Male , Ossification, Heterotopic , Osteosarcoma/diagnostic imaging , Osteosarcoma/secondary , Radiography, Thoracic , Tomography, X-Ray ComputedSubject(s)
Adolescent , Bone Neoplasms/drug therapy , Bone Neoplasms/diagnostic imaging , Bone Neoplasms/surgery , Chemotherapy, Adjuvant , Combined Modality Therapy , Femur/pathology , Humans , Male , Neoadjuvant Therapy , Neoplasms, Second Primary/etiology , Neoplasms, Second Primary/pathology , Osteosarcoma/drug therapy , Osteosarcoma/diagnostic imaging , Osteosarcoma/secondary , Osteosarcoma/surgery , Tomography, X-Ray Computed , Treatment OutcomeABSTRACT
Introducción. Es bien conocido que el osteosarcoma se presenta frecuentemente como segunda neoplasia del retinoblastoma congénito, así como otro tipo de carcinomas, melanomas y tumores neuroepiteliales. Todos los pacientes con retinoblastoma bilateral congéntio presentan una alteración del gen RB1 localizado en el cromosoma 13q14. Caso clínico. Se presenta el caso de una paciente con retinoblastoma bilateral congénito diagnosticado a la edad de 1 año 11 meses, quien recibió tratamiento con ciclofosfamida, epirrubicina y VP 16, entre otros agentes; y que desarrolló osteosarcoma peroneo con metástasis pulmonares tras una latencia de 10 años 6 meses. En esta paciente es conocido el uso de alquilantes, antracíclicos y etopósido, así como los antecedentes familiares de cáncer por ambas ramas. Conclusión. El retinoblastoma bilateral conlleva factores de riesgo para el desarrollo de segundas neoplasias. Los antecedentes familiares constituyen razones suficientes para catalogarlo como un síndrome de cáncer familiar, el uso de agentes alquilantes, antraciclicos y etopósidos, aumentan este riesgo acortando el período de latencia
Subject(s)
Humans , Female , Adolescent , Genes, Retinoblastoma , Osteosarcoma/diagnosis , Osteosarcoma/secondary , Retinoblastoma/complications , Retinoblastoma/congenital , Retinoblastoma/drug therapy , Retinoblastoma/genetics , Genetic Diseases, Inborn/geneticsABSTRACT
Secondary osteosarcomas of the maxilla/premaxilla are extremely rare. We describe a case of a 24-year-old man with a metastasis of osteosarcoma to the premaxilla. The patient had undergone hip disarticulation followed by adria based chemotherapy for chondroblastic osteosarcoma of the proximal right femur ten years ago. The metastatic work up did not show liver or lung metastasis. The patient underwent premaxillectomy in February 1999. The histopathology revealed a chondroblatic osteosarcoma consistent with metastasis in the clinical setting. The patient was disease free at the end of five months.
Subject(s)
Adult , Bone Neoplasms/pathology , Femur , Humans , Male , Maxillary Neoplasms/secondary , Neoplasm Metastasis , Osteosarcoma/secondaryABSTRACT
El sarcoma osteogénico u osteosarcoma es un tumor maligno de huesos que ocurre en la segunda o tercera década de vida y en muy raras ocasiones ocurre en niños menores de 5 años. Presentamos el caso de un niño de 3 9/12 años de edas, uno de los pacientes más jóvenes descritos en la literatura. En este paciente el tumor fue muy agresivo, con metástasis al pulmón al momento del diagnóstico y una progresión muy rápida a pesar de quimioterapia y cirugía. El diagnóstico de este paciente fue algo tardío por la presentación poco usual a esta edad
Subject(s)
Child, Preschool , Child , Humans , Male , Femoral Neoplasms/diagnosis , Lung Neoplasms/secondary , Osteosarcoma/diagnosis , Lung Neoplasms , Magnetic Resonance Imaging , Osteosarcoma/secondary , Tomography, X-Ray ComputedABSTRACT
Se estudia la historia clínica de una niña en quien se practicó la amputación transescápulotorácica izquierda, cuando tenía siete años de edad, por sarcoma osteogénico en húmero izquierdo; y se le prescribió un tratamiento a base de Ciclofosfamida-Methotrexate-Adriamicina, en ciclos mensuales, durante un año; y durante dos ciclos más, con Methotrexate y Ciclofosfamida. La paciente permaneció bien hasta que presentó, ochenta y ocho meses después, una lesión en el tercio distal del muslo derecho; la biopsia reveló que era un Sarcoma osteogénico. Se le amputó el miembro inferior derecho y se prescribió Ciclofosfamida y Decarbacina. Como tres meses después presentara metástasis en los pulmones, se le administró CisPlatinum. La paciente falleció un año después de la segunda operación. Los autores consideran que la niñita sufrió, por las razones que comentan, de un foco primario de sarcoma osteogénico en 1978, del cual aparentemente curó; y de un segundo foco primario de sarcoma osteogénico con metástasis pulmonar, en 1985
Subject(s)
Child , Humans , Female , Osteosarcoma/secondary , Femoral Neoplasms/secondary , Neoplasms, Multiple Primary , Humerus , Osteosarcoma/pathology , Osteosarcoma/therapy , Femoral Neoplasms/pathology , Amputation, Surgical , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Diagnosis, DifferentialABSTRACT
Os autores apresentam três casos de pacientes portadores de retinoblastoma, tratados com químio e radioterapia, que desenvolveram segunda neoplasia 4, 10 e 13 anos após o primeiro diagnóstico. Discutem-se efeitos carcinogenéticos da terapêutica antineoplásica (rádio e quimioterapia), e a susceptibilidade genética de mutaçäo que leva os portadores de uma determinada neoplasia a desenvolverem uma segunda (cuja etiologia encontra-se em investigaçäo). Com base em revisäo da literatura e na experiência pessoal, podemos dizer que há evidência clara de que os portadores de retinoblastoma bilateral têm maior probabilidade de manifestar segundo tumor. Nos três casos analisados os tumores secundários foram osteossarcoma em dois casos, e fibrossarcoma em um caso e todos eram portadores de retinoblastoma bilateral. No intuito de alertar para esse fato, propö-se uma menor exposiçäo dos pacientes portadores de retinoblastoma bilateral a tratamentos potencialmente agressivos. O acompanhamento dos pacientes deve ser ser longo, uma vez que os segundos tumores podem ocorrer vários anos após o término do tratamento da primeira neoplasia