Effect of genistein on the pancreas of streptozotocin-induced diabetic male rats: light and electron microscopic study

Diabetes mellitus is a worldwide serious health problem. The critical need for novel therapeutic approaches to treat diabetes mellitus is clear. Genistein, a natural soy isoflavone, have numerous health benefits attributed to multiple biological functions. To investigate the effect of genistein on the structure of pancreatic beta cells and acinar cells in streptozotocin-induced diabetic rats. Thirty adult male albino rats were classified into: group I [control], group II in which diabetes was induced by a single intraperitoneal injection of streptozotocin [STZ] [80 mg/kg] and group 111 in which the diabetic rats were injected subcutaneously with genistein [0.25 mg/kg/day] Alter 3 months, blood glucose concentrations were assessed and pancreas specimens were processed lor light and electron microscopic study. Immunohistochemical insulin reactivity and morphometric analysis of the islet diameter were also studied. STZ, in group II rats, caused shrinkage of the pancreatic islet and induced beta cell damage in addition to weak insulin immunoreactivity and elevated blood glucose level. Many Icinar cells of this group showed accumulated zymogen granules, pleomorphic mitochondria and small lipid droplets. Genistein, in group III rats, preserved beta cell mass as evidenced by the large islets with strong insulin immunoreactivity and the significant reduction in blood glucose level. Ultrastructurally, beta cells of group III rats had numerous secretory granules and well developed Golgi bodies. Iknvever, the acinar cells of genistein treated rats exhibited more structural changes than group II with loss of the normal polarity and marked damage of mitochondria. Zymogen granules exhibited low electron density with frequent docking to the lateral plasma membrane and granule-granule fusion. Genistein protected the beta cells against STZ-induced damage. However its deleterious effect on the pancreatic acinar cells might limit its benefit as a promising therapy for diabetes mellitus

effects of Ambrosia maritime L. [damsissa] on some biochemical and histological parameters of diabetic albino rats

Diabetes mellitus is one of the common and widely distributed metabolic diseases all over the world. This disease is characterized by hyperglycemia that results from defects in insulin secretion, insulin action or both. Different medicinal plant species are used as a traditional treatment for diabetes mellitus e.g. Ambrosia maritima, L. [Damsissa] which is one of these plants that its extract was used to treat diabetic patients long times ago. This work was aimed to investigate the antidiabetic, hypolipidemic and antioxidant effects of the aqueous extract of Ambrosia maritima, L. [Damsissa] on the alloxan-induced diabetic male albino rats. This study was performed on thirty male albino rats with an average 100-110 g body weight. The animals were divided into three groups [10 /cage]; Group I [Control untreated-group], Group II [Alloxan-induced diabetic group] and Group III [diabetic group treated orally with "28.5 mg/ kg body wt. twice/ day" of the plant extract]. The biochemical results showed marked decline [p<0.01] in the levels of the serum insulin, body weight, total proteins, albumin, globulin and HDL accompanied with marked elevation [p<0.001] in the levels of fasting blood glucose, levels of HOMA_IR, AST, ALT, GGT, urea, creatinine, uric acid, serum TC, TG, LDL, VLDL and ratios of TC/HDL and LDL/HDL [risk factors] in diabetic rats in comparison with the control group. Daily management of the diabetic rates with aqueous extract of Damsissa showed significant improvement in most of these parameters. Histologically, considerable improvement in the morphological changes that was observed in diabetic groups had been detected after treatment with Damsissa in liver, kidney and pancreatic tissues in comparison to the control group. It could be concluded that Ambrosia maritima, L. [Damsissa] can be used as an antidiabetic drug that can lower blood glucose concentration and guard against the negative effects of diabetes

effects of experimental aflatoxicosis on the pancreas of adult male albino rats and the role of ginger supplementation: a histological and biochemical study

Aflatoxin contamination of foods is a worldwide problem, especially in developing countries. Ginger has antioxidant properties. To study the histological and biochemical changes in the pancreas of rats with experimental aflatoxicosis, and to evaluate the role of ginger supplementation. Forty-five adult male albino rats were equally divided into three groups. Group I that served as the control group. Group II that received 250 119/kg body weight/day of aflatoxin B1 dissolved in olive oil using a gastric tube for 5 days/week for 4 weeks. Group III that received both aflatoxin as in group II and 400 mg/kg body weight/day of ginger orally for 5 days/week for 4 weeks. At the end of the experiment, all rats were anesthetized, and their pancreases were extirpated and divided into two parts to be processed for light and electron microscopic examinations. Morphometrical analysis for area percentage of collagen fibers and biochemical analysis for glucose, insulin, and serum amylase were performed and statistically analyzed. Examination of group II revealed thick interlobular septa that contained congested blood vessels, cellular infiltration, mast, and fat cells. Pancreatic acinar cells showed decreased secretory granules, vacuolization, and dilated fragmented rough endoplasmic reticulum. Few acinar cells showed rarified areas of cytoplasm. Some acinar cells had small condensed heterochromatic nuclei. Most of the islets of Langerhans were formed of cells separated by dilated congested capillaries. Most of the nuclei of beta cells were euchromatic, whereas some were small heterochromatic. The cytoplasm of beta cells had a variety of secretory granules. Most of them had an electrondense core and an electron-lucent halo, whereas others had homogenous moderate density. Some granules coalesced. A few cells had cytoplasmic areas depleted of granules. Pancreatic ducts were dilated. Examination of group III revealed that pancreatic lobules were separated by thin interlobular septa. Acini had numerous apical acidophilic secretory granules, a few vacuoles, and basal euchromatic nuclei. Beta cells of the islets of Langerhans had euchromatic nuclei and numerous secretory granules with an electron-dense core and a wide electron-lucent halo. Biochemical analysis of glucose and serum amylase showed a highly significant increase, whereas that of insulin showed a highly significant decrease, in group II in comparison with group I. The glucose and serum amylase levels were significantly decreased, whereas the insulin level was significantly increased in group III compared with group II. Aflatoxin had a deleterious effect on the histological structure of the rats' pancreas, and ginger minimized these effects

Therapeutic effect of pentoxifylline versus losartan on experimentally induced acute pancreatitis in adult albino rats: light and electron microscopic study

To study the therapeutic benefit of pentoxifylline versus losartan on L-arginine-induced acute pancreatitis in adult albino rats. Forty adult male albino rats were used and divided into a control group I, group II which received L-arginine, group III which received L-arginine followed by pentoxifylline and group IV which received L-arginine followed by losartan. Serum amylase and lipase assay was carried out and subjected to statistical analysis. Pancreatic tissue samples were taken and processed for light and transmission electron microscopic examination. Rats that received L-arginine [group II] showed loss of acinar architecture, edema, and zonal degenerative changes. Pentoxifylline-treated animals [group III] revealed apparent small-sized acini with a small amount of electron-lucent secretory granules and the edema was still present in some areas. Rats treated with losartan [group IV] showed an almost normal pancreatic architecture. Most of the acinar cells had normal apical electron-dense zymogen granules, normal rough endoplasmic reticulum, and apparently normal mitochondria. Moreover, there was significant reduction in serum amylase and lipase levels in the losartan-treated group compared with the pentoxifylline-treated group. Losartan was more efficient than pentoxifylline in the treatment of acute pancreatitis induced by L-arginine as indicated by histological and biochemical results

Effect of simvastatin on the exocrine part of the pancreas in adult albino rat and the possible protective role of coenzyme Q10: a light and electron microscopic study

The statins represent the drugs of choice for treatment of hypercholesterolaemia. Because of the common use of statins [including simvastatin], both physicians and patients have demonstrated valid concerns about the safety associated with the use of such medications. This work was performed to study the effect of simvastatin on the exocrine part of the pancreas and to evaluate the possible protective role of Coenzyme Q10 [CoQ10]. The present study was carried out on 35 adult male albino rats which were divided into; group I [control group], group II [given 1.44 mg of simvastatin once daily for 12 weeks] and group III [given simvastatin in the same dose concomitantly with 3.6 mg of CoQ10 once daily for the same period]. The specimens were prepared for light and electron microscopic examination. Sections of simvastatin-treated rats showed morphological changes in acinar cells in the form of pyknotic nuclei, cytoplasmic vacuolation, abnormal shape of acini, congestion of blood vessels and widening of interstitial tissue. By Verhoeff's Van Gieson's stain, dissolution of elastic laminae was detected in some blood vessels. Ultrastructurally, there were variation of electron density of zymogen granules, dilation of both RER and perinuclear space, large vacuoles and damaged mitochondria in some acinar cells. The above findings were less prominent in animals treated with both simvastatin and CoQ10. Simvastatin has a harmful effect on the exocrine part of the pancreas and it is advisable that patients receiving simvastatin could use CoQ10 to minimize its side effects

Histological study on the possible protective role of vitamin C against endotoxin on pancreatic acini of adult male albino rat

Endotoxin is a lipopolysaccaride molecule derived from Gram-Ve bacterial cell wall. It acts as a potent signaling molecule which elicits a systemic inflammatory response syndrome [SIRS] defined as sepsis. This syndrome is considered to be the most common cause of morbidity and mortality in intensive care units. Dietary antioxidant vitamins could protect against endotoxin damage. The present study was conducted to illustrate the histological changes in pancreatic acini of albino rat in response to endotoxin administration and the possible protective role of vitamin C. In this study twenty four adult male albino rats were used and divided into four groups, [six rats each]. Group I [Control group]. Group II [Vitamin C treated group] was given vitamin C orally in a dose level of 18 mg/kg body weight once daily for two weeks. Group III [Endotoxin treated group] was given single dose of endotoxin at a dose level of 5 mg/kg body weight intraperitoneally. Group IV [Protective group] was given oral vitamin C in a dose level of 18 mg/kg body weight daily for two weeks prior to endotoxin administration [5mg/kg] as in group II. Microscopical examination of H and E stained sections of the endotoxin treated group [group III] showed severely affected pancreatic acini with loss of the normal architectural pattern. Ultrastructurally, dilatation of rough endoplasmic reticulum, cytoplasmic vacuolation, zymogen granule depletion and loss of polarity were the commonly encountered lesions in association with nuclear changes. In Group IV vitamin C reduced endotoxin toxicity to pancreatic acini as revealed by presentation of the pancreatic architecture to be nearly similar to that of the control group. It is concluded that vitamin C had a protective effect to pancreatic acini against endotoxin induced pancreatitis

Effects of aging on pancreatic islet cell function: an experimental immunohistochemical and ultrastructural study

A strong relationship between aging and diabetes mellitus has been clinically suggested, however, none of the previous published data had clearly focused on the age-related cytomorphological changes in the pancreas which are the goal of this study. Three groups of male apparently healthy rabbits have been used, ten animals each; classified as group-1 [3-5months old]; group-2 [9-12 months old] and group-3 [24-36 months old]. After sacrification, sections from the pancreas were stained by Haematoxylin and Eosin [H and E], Gomori trichromic stain and ultrastructurally to detect aging histologic changes as well as immunohistochemically to identify insulin and glucagon secreting cells using their appropriate monoclonal antibodies. A progressive histological distortion with fibrosis and fatty changes were directly proportional to age, being mild in group-2 and severe in group-3. Morphometric studies by computerized image analysis showed that the mean number of islets was significantly higher in group2 [8.98+/-1.51], lowest in group-1 [5.08+/-1.48] and intermediate in group-3 [6.37+/-1.37]. The mean diameter and square area of islets were significantly higher in group-2 compared to other groups [P< 0.05]. The mean number of beta cells per islet and their secretary granules were significantly [P <0.05] higher in group-2, intermediate in group-1 and lowest in group-3.In contrast, the mean number of alpha cells per islet and their secretory granules were insignificantly [P< 0.05] higher in group -2, intermediate in group-3 and lowest in group-1.Also, the beta/alpha ratio [beta cells/alpha cells] was greatest in group-2 [3.059:1], intermediate in group-1 [3.37:1], and lowest in group-3 [2.479:1]. The increased number of beta cells may be due to a compensatory process to correct the hormonal feedback mechanism of insulin .The results of this work suggest that beta cells are generally more vulnerable to aging, an observation which might be correlated clinically with higher incidence of diabetes in older ages

Could cinnamaldehyde be harnful? histological, cytogenetical and biochemical studies on the effects on some organs of mice

Cinnamaldehyde [CNMA] is present naturally as cinnamon tree. Because of its widespread use as fragrance additive, it was chosen for the current study. Sixty mice were divided into 6 equal groups. First three groups were control groups and the other three groups received CNMA daily, orally in three dose regimens; 1/2 LD50, 1/4 LD50 and 1/8 LD50 for 30 consecutive days, then animals were sacrificed; Liver function tests and liver tissue glutathione concentration were determined. Pancreatic specimens and olfactory mucosa were processed for both light and transmission electron microscopic studies and for scanning electron microscopic study respectively. Bone marrow cells micronucleus and chromosomal aberration tests were performed for cytogenetical study. cinnamaldehyde, in a dose related manner resulted in abnormal nuclear morphology and hyperchromatasia with increased nuclear/cytoplasinic ratio of the pancreatic acinar cells and decreased tendency for acinar formation, suggesting pancreatic acinar dysplasia. Atrophy of receptor cells of olfactory mucosa with diminished surface processes was observed in mice received 1/2 LD50 cNMA. cinnamaldehyde induced dose dependent increase in the frequency of both micro nucleated polychromatic erythrocytes and chromosomal aberrations. Significant elevation of the liver enzymes, total bilirubin and a significant reduction of the liver glutathione concentration were also detected in a dose related manner in conclusion, the considered toxicity assay parameters had shown a correlation between the administered dose of CNMA and its deteriorative effects. So, it is recommended that CNMA should not be consumed in a dose more than the acceptable daily intake and its use as flavoring agent should be kept as low as possible

Effect of valproic acid on the pancreatic acini of the adult rats and the possible protective role of L-carnitine. light, electron microscopy and DNA electrophoresis

Valproic acid [VPA] is a commonly prescribed medication used for epilepsy, migraine and bipolar disorder. Although the common adverse effects associated with VPA are typically benign, pancreatitis may occur. L-carnitine is a naturally occurring compound widely distributed in all cells. It has neurotropic, neuroprotective and antioxidant properties. The aim of this study was to demonstrate the effect of VPA [Depakine] on the exocrine part of pancreas when used alone and when given concomitantly with L-carnitine. In this study twenty four adult male albino rats were selected and divided into four groups. Group I [GI] control group. Group II [G II], were given L-carnitine [100mg/kg]. Group III [G III], were given VPA [200 mg/kg]. Group IV [GIV], were given both L-carnitine and VPA in the same previous doses. Both drugs were given orally once daily for three months. The results obtained in group III showed variable degrees of acinar degeneration and cellular infiltration between the acini. The collagen fibers around the B. V. were increased. Ultrastructurally, there were dilatation of [r] ER and Golgi apparatus, focal destruction of mitochondria, increased number of secondary lysosomes, decreased or even depletion of zymogen granules and nuclear changes. Fat droplets in the basal part of acinar cells, cytoplasmic vacuoles as well as DNA damage were also noticed. On the otherhand, pancreatic sections of animals of [G IV] that were treated with VPA and L-carnitine showed marked reduction of the previous cellular changes and the cytoplasmic organelles were slightly affected. In conclusion the present study revealed that L-carnitine can be used concomitantly with VPA to minimize its adverse effects on the exocrine portion of pancreas

Fine structural studies on the protective role of guanidinoethane sulfonic acid [GES] against low dose: Gamma radiation induced damage in rat pancreas

Any radiation exposure, no matter how small, carries with it some risk. All cells are not equally sensitive to radiation damage. The biological effects of high levels of radiation exposure are fairly well known, but the effects of low levels of radiation are more difficult to determine. Radio protective effects of Guanidinoethane Sulfonic Acid [GES] as analogue of taurine have been investigated in whole body irradiated rats. This study was taken to evaluate the structural effect of low dose radiation on the pancreatic cells as a model of radiosensitive organs and the role of [GES] as a radioprotector. Male rats were chosen for this study. The animals were divided into three groups each had equal numbers of animals, the first group served as a control group. The second group was exposed to gamma radiation at a dose level 1 Gy twice a week for 12 weeks, at a dose rate 1 Gy/1.5 min. The third group was given Guanidinoethane Sulfonic Acid [GES] as 1% solution in drinking water beginning 14 days before irradiation and continuing for 14 days thereafter, when the rats were killed. Histopathological and electron microscopical studies revealed that accumulative dose of gamma radiation affects both exocrine and endocrine cells of the pancreas. Using Guanidinoethane Sulfonic Acid [GES] as an analog of taurine helps the cells to regain its normal structure

Effect of rofecoxib [a new selective cox-II inhibitor] on plasma insulin level and pancreatic free hydroxyl radicals in diabetic rats

This study is designed to demonstrate the effect of a newly introduced selective COX-2 inhibitor, Rofecoxib, on streptozoto-cin-induced diabetes and whether it possesses an anti-oxidant effect or not. The effects of this drug were compared to those of the relatively selective COX-I inhibitor, indomethacin. The animals were divided into four groups, twenty rats each. The first group served as normal control; the second group received streptozotocin alone [45 ing/kg, i.p.] while the remaining two groups were given the same dose of streptozotocin after being treated orally for ten days with either 2.5 nig/kg/day of "Rofecoxib" [in the third group] or 1 mg/kg/day of indomethacin [in the fourth group]. Statistical analysis of the results revealed that animals treated with streptozotocin [STZ] alone exhibited significant plasma insulin reduction [both basal and half an hour after glucose load] while those treated with Rofecoxib showed insignificant reduction in plasma insulin level following STZ, in comparison to the control group. Histopathological changes revealed dial in the STZ group, there was depletion of insulin secretory granules while in the Rofecoxib group, they appear more or less normal. In the STZ group, there was a significant rise in the pancreatic malondialdehyde [MDA] level while in the Rofecoxib group there was insignificant elevation following streptozotocin, in comparison to control group. Treatment of animals with indomethacin [the 4 group] failed to protect them against the destructive effects of streptozotocin. It is concluded that, in contrast to indomethacin, Rofecoxib has beneficial effects in insulin dependent diabetes mellitus [IDDM] both by its modulatory effects on insulin secretion and its antioxidant properties

Pancreatic involvement in co-infection visceral leishmaniasis and HIV: histological and ultrastructural aspects

The involvement of the gastrointestinal tract in the co-infection of HIV and Leishmania is rarely reported. We report the case of an HIV-infected adult man co-infected with a disseminated form of leishmaniasis involving the liver, lymph nodes, spleen and, as a feature reported for the first time in the English literature, the pancreas. Light microscopy showed amastigote forms of Leishmania in pancreatic macrophages and immunohistochemical staining revealed antigens for Leishmania and also for HIV p24. Microscopic and ultrastructural analysis revealed severe acinar atrophy, decreased zymogen granules in the acinar cytoplasm and also nuclear abnormalities such as pyknosis, hyperchromatism and thickened chromatin. These findings might correspond to the histologic pattern of protein-energy malnutrition in the pancreas as shown in our previous study in pancreas with AIDS and no Leishmania. In this particular case, the protein-energy malnutrition may be due to cirrhosis, or, Leishmania or HIV infection or all mixed. We believe that this case represents the morphologic substratum of the protein energy malnutrition in pancreas induced by the HIV infection. Further studies are needed to elucidate these issues

Effect of oxytetracycline on the postnatal development of the pancreas in rabbit: a light and electron microscopic study

Sixteen pregnant Californian rabbits were used in this study and divided into control and treated groups. The later group was injected with tetracycline from the 11th to the 15th day of pregnancy by a single daily dose [5 mg/kg]. Small fragments from the tail of the fetal pancreas at 3 and 20 days postnatally were taken and processed for EM examination. The exocrine and endocrine parts of the control fetal rabbit pancreas reached the adult form at the 20th day after birth as detected by light and EM examination. The study advised the avoidance of tetracycline in pregnant women throughout the period of pregnancy

O pâncreas na desnutriçäo protéico-calórica na infância / Pancreatic dysfunction in protein-energy malnutrition in children

A disfunçäo pancreática tem sido frequentemente associada à desnutriçäo protéico-calórica e aparece mais frequentemente quando há uma ingesta relativamente alta de alimentos ricos em amido, com deficiência na ingesta de proteínas. As alteraçöes pancreáticas säo globais. Há diminuiçäo do número e tamanho de algumas organelas intracelulares, com marcada depressäo nos níveis e na atividade das enzimas lipase, amilase ,tripsina, quimotripsina e ribonuclease, além de atrofia, fibrose e calcificaçöes do órgäo. Hipoalbuminemia, hiperglobulinemia e esteatorréia säo achados também frequentes em pacientes com desnutriçäo. A dosagem de tripsinogênio catiönico sérico é um método bastante acurado para o diagnóstico de insuficiência pancreática. Atualmente, tem-se dado destaque para o esteatócrito como método de triagem. Com o aporte dietético adequado, a deficiência enzimática desaparece progressivamente, na maioria das vezes näo sendo necessária a utilizaçäo de enzimas pancreáticas exógenas

Estudo morfológico e estereológico do desenvolvimento pós-natal do pâncreas da cobaia (Cavia porcellus) / Morphological and stereological study of the posnatal development of guinea pig pancreas (Cavia porcellus)

O desenvolvimento pós-natal do pâncreas da cobaia (Cavia porcellus) foi estudado por métodos estereológicos ao microscópio de luz, no período de 2 a 140 dias de idade. As modificaçöes morfométricas detectadas no estudo dos dados numéricos foram acompanhadas por uma análise morfológica qualitativa. A massa pancreática exibiu um aumento acentuado de 805 por cento, no período de 2 a 140 dias de vida pós-natal. Este crescimento pode ser expresso pela equaçäo linear Y = 519,2 + 14,2x (coeficiente de correlaçäo r= 0,95) e o tempo de duplicaçäo calculado por essa equaçäo foi de 38,6 dias. Este crescimento de massa pancreática ocorreu devido ao aumento de volume em todos os compartimentos morfológicos, notadamente o dos ácinos e o das outras estruturas (estroma). A relativa estabilidade da densidade de volume dos vários compartimentos mostrou que durante este crescimento as relaçöes volumétricas säo mantidas. O volume do compartimento dos ácinos aumentou 756 por cento, no período total analisado. Este crescimento pode ser representado pela equaçäo Y = 204,1 + 4,7x (r= 0,92) e o tempo de duplicaçäo calculado para o período de 2 a 140 dias foi de 45,6 dias. Este aumento de volume do compartimento dos ácinos ocorreu por dois mecanismos de crescimento: a atividade proliferativa e o aumento de volume celular. O estudo da evoluçäo do número absoluto de células acinosas mostrou um aumento de 361 por cento, no período de 2 a 140 dias. A equaçäo obtida pela análise de regressäo para exprimir esse aumento foi Y = 110,1 + 1,6x (r=0,94) e o tempo de duplicaçäo calculado foi de 70,8 dias. Por outro lado, o volume celular médio das células acinosas aumentou 210 por cento, no mesmo período. A equaçäo linear obtida foi Y = 755,3 + 6,4x (r=0,86) e o tempo de duplicaçäo foi de 120,0 dias. Os dados mostraram que no período total de 2 a 140 dias, o aumento de volume do compartimento dos ácinos ocorreu com um predomínio de atividade proliferativa sobre aumento de volume celular

effect of propanolol [inderal] on the exocrine pancreas of rat: a histological and ultrastructural study

This study was done to investigate the effect of propanolol [inderal] on the exocrine pancreas of rats. Ten adult male sparague-Dawley rats were used. Five rats were used as control and the other five were injected daily i.p. with propanolol in a dose of 0.01 mg/100 of rat body weight for 5 weeks. Animals were sacrificed, and the pancreas was fixed in formalin and gluteraldehyde for light and electron microscopic examination. The propanolol-treated pancreas showed marked fibrous thickening of the capsule and connective tissue septa. Vascular changes, acinar disruption and ductular proliferation were also evident. The ultrastructural picture of the propanolol treated acini showed dilatation and vesiculation of the rER. Zymogen granules of medium density, widening of the acinar lumen and decreasing numbers of projecting microvilli were apparent. In conclusion the ischemia induced by propanolol injection resulted in pancreatitis of the treated rats

Ultrastructural study of cell injury induced by coxsackievirus B3 in pancreatic and cardiac tissues

Con fines de una mejor caracterización de la injuria celular provocada por el coxsackievirus B3 en páncreas y corazón, ratones Balb/C ya destetados fueron intraperitonealmente inoculados con una variante miocardítica de ese serotipo viral. A nivel ultraestructural, las células pancráticas acinosas mostraron evidente distorsión, evidenciada por acentuada pérdida de organelas y de gránulos de zimógeno, así como apreciable dilatación del retículo endoplásmico granuloso. En cuanto a las células cardíacas, las alteraciones citoesqueléticas fueron severas, llegando a incluir colapso de miofibrilas con disminuición de su número e irregularidad en su disposición. Además, los cardiomiocitos exhibieron cromatina de distribución irregular y mitocondrias de tamaño agranado. A la vez de los focos necróticos tendían a desaparecer, el número de fibroblastos aumentaba en forma coincidente a la progresiva cicatrización de la lesión. Los cambios ultraestructurales observados en páncreas y corazón fueron concomitantes en consecuencia que el daño celular era atribuíble a la acción directa del virus

Efeito do raio laser CO2 no pancreas de cäes: estudo a microscopia óptica e eletrônica de varredura / Effects of CO2 laser on the pancreas of dogs: study at light and scanning electronic microscopy

Foi realizado um estudo experimental em cäes com o objetivo de pesquisar o efeito do raio laser CO2 em comparaçäo com o bisturi comum na incisäo do parênquima pancreático. Foram operados quatro animais nos quais efetuou-se incisöes transversais no pâncreas: duas com o emprego do laser e uma com bisturi. As superfícies de corte foram estudadas à microscopia óptica e eletrônica de varredura. Constatou-se que a aplicaçäo do raio laser CO2 no pâncreas de cäes produziu uma evaporaçäo tecidual, resultando uma superfície irregular de material necrótico carbonizado com sulcos e forames, e em profundidade, camadas de células rotas com citoplasma coagulado, núcleos picnóticos e ácinos distorcidos, entremeados de espaços vazios resultantes da vaporizaçäo de células acinares. Os vasos e ductos pancreáticos se apresentam obliterados por um material amorfo eosinofílico resultante da coagulaçäo do tecido. A espessura da camada alterada pela açäo do laser é em média de 156,40 micrômetros. A secreçäo com o bisturi produz uma superfície lisa e regular com apenas uma camada de ácinos de creas de menor tamanho e algumas rupturas celulares. Concluiu-se que o emprego do raio laser CO2 no pâncreas de cäes provocou necrose e reaçäo tecidual mais intensa, porém obliterou pequenos vasos e ductos coletores

Avaliaçäo histológia e bioquímica da pâncreas exócrina em ratos submetidos a ressecçäo ou exclusäo jejunoileal extensas / 123-8

Foram investigados os efeito da ressecçäo intestinal de 80% de jejuno-íelo e exclusäo jejunoileal da mesma extensäo no pâncreas exócrino de ratos. Após período de observaçäo de 100 dias, foram avaliados curva ponderal, ingestäo diária de alimentos, peso do pâncreas, relaçäo peso do pâncreas/peso do animal, exames histológico (macroscopia óptica), com e sem testes histoquímicos, ultra-estrutural e provas bioquímicas. Os ratos submetidos a ressecçäo ou a exclusäo intestinal perderam o seu peso inicial que foi recuperado somente após 30 e 60 dias, respectivamente. Após esse período os animais ganharam peso progressivamente. A ingestäo alimentar foi semelhante nos grupos experimentais e controle. O peso pancreático, no final do período experimental nos animais controle, foi de 0,44 ñ 0,04g. Nos aniamis que sofreram a ressecçäo ou exclusäo intestinal, o peso pancreático foi significativamente superior, 0,54 ñ 0,8g e 0,54 ñ 0,11. A histometria revelou, nos animais operados, um número significativamente maior de células acinares, sugerindo que o ganho de peso pancreático se deve a hiperplasia do órgäo. Por outro lado, os exames histoquímicos evidenciaram alteraçöes na composiçäo pancreática. Verificamos que as células acinares dos ratos submetidos a ressecçäo e exclusäo intestinal produzem mais intensamente grânulos de secreçäo (zimogênio) sem conseguirem, no entanto, armazená-los. A microscopia eletrônica confirmou os resultados obtidos pelos métodos histoquímicos. A concentraçäo dos grânulos de z