Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add filters








Language
Year range
1.
Indian J Exp Biol ; 2014 Nov; 52(11): 1090-1097
Article in English | IMSEAR | ID: sea-153795

ABSTRACT

The present study describes a novel and simple vaccination strategy that involve culturing of M. tuberculosis in the macrophage cells. Isolation of phagosome from macrophage (cell line J774) infected with M. tuberculosis (H37) and M. bovis (BCG) at early and late phase of infection was done ensuing the identification and characterization of these phagosome. In vitro study of apoptosis induced by phagosome infected with (H37) and (BCG) was performed. The vaccine candidate with H37 MOI- 1:10 at 3 h, MOI- 1:20 at 1, 1.5, 2.5 and 3 h and BCG MOI- 1:20 at 3.5 h showed percentage apoptosis as 38.64, 39.93, 34.66, 22.56,34.59 and 37.81% respectively. The results designates that macrophages provide cellular niche during infection and illustrate considerable immunogenic property. Novel antigens expressed or secreted by H37 in infected macrophages can provide evidence to be a successful vaccine candidate as it endures enhanced immune response than BCG.


Subject(s)
Animals , Antigens, Bacterial/immunology , Apoptosis , Cell Line, Tumor , Culture Media , DNA Fragmentation , Lymphoma, Non-Hodgkin/pathology , Macrophages/microbiology , Mice , Mice, Inbred BALB C , Mycobacterium bovis/growth & development , Mycobacterium bovis/immunology , Mycobacterium tuberculosis/growth & development , Mycobacterium tuberculosis/immunology , Phagosomes/immunology , Phagosomes/microbiology , Tuberculosis Vaccines/immunology , Tuberculosis Vaccines/isolation & purification
2.
Mem. Inst. Oswaldo Cruz ; 104(2): 267-272, Mar. 2009. ilus
Article in English | LILACS | ID: lil-533516

ABSTRACT

Toxoplasmagondii represents a pathogen that survives within host cells by preventing the endosomal-lysosomal compartments from fusing with the parasitophorous vacuoles. The dogma had been that the non-fusogenic nature of these vacuoles is irreversible. Recent studies revealed that this dogma is not correct. Cell-mediated immunity through CD40 re-routes the parasitophorous vacuoles to the lysosomal compartment by a process called autophagy. Autophagosome formation around the parasitophorous vacuole results in killing of the T. gondii. CD40-induced autophagy likely contributes to resistance against T. gondii particularly in neural tissue.


Subject(s)
Animals , Humans , /immunology , Autophagy/immunology , Toxoplasma/immunology , Toxoplasmosis/parasitology , Host-Parasite Interactions/immunology , Lysosomes/immunology , Phagosomes/immunology , Toxoplasmosis/immunology
SELECTION OF CITATIONS
SEARCH DETAIL