ABSTRACT
BACKGROUND: To identify the optimum culture conditions by investigating isolated rat hepatocytes cultured in medium containing different growth factors. METHODS: Hepatocytes were isolated from rats using a two-step perfusion technique and divided into the following four groups cultured in medium containing different growth factors: control, epidermal growth factor (EGF), insulin, and EGF+insulin. The viability of the cultured rat hepatocytes and liver function parameters, including albumin, ammonia, and urea in the culture medium, were measured. Hepatocyte morphology was examined by staining with hematoxylin and eosin, and albumin receptor expression was confirmed by immunofluorescence. RESULTS: Slightly higher viability was observed in the growth factor groups than in the control group, although without significance (P=0.073). The levels of albumin (P=0.001), ammonia (P<0.001), and urea (P=0.041) differed significantly among the four groups. The functional parameters in the growth factor groups, particularly the EGF+insulin group, were significantly superior to those in the control group. The morphology of the hepatocytes in all growth factor groups was well maintained at 10 days. However, the control group showed deterioration in cell morphology by day 7. CONCLUSIONS: Morphological and functional assessment indicated that the presence of growth factors, particularly EGF+insulin, provided culture conditions superior to those of non-supplemented medium.
Subject(s)
Animals , Rats , Ammonia , Eosine Yellowish-(YS) , Epidermal Growth Factor , Fluorescent Antibody Technique , Hematoxylin , Hepatocytes , Insulin , Intercellular Signaling Peptides and Proteins , Liver , Perfusion , Receptors, Albumin , UreaABSTRACT
Introdução: A reanimação fluídica da criança queimada é um desafio devido à intolerância à insuficiente ou excessiva oferta de líquidos. Há dúvidas em relação à utilização de solução coloide na ressuscitação volêmica e também quanto ao melhor momento a ser administrada. O momento ideal para a administração de albumina permanece em foco de debate, se deveria ser utilizada como estratégia de resgate, quando o volume de cristaloide infundido se torna excessivo, ou rotineiramente, como intervenção primária em pacientes com queimaduras extensas. Objetivos: Avaliar e comparar quanto a evolução clínica de crianças com lesões térmicas que receberam abordagem de infusão precoce (entre 8 e 12 horas do acidente) de solução coloide natural versus crianças que receberam abordagem de infusão tardia (após 24 horas do acidente) da mesma solução para reanimação na fase aguda. Metodologia: Ensaio Clínico Randomizado Controlado, realizado no Centro de Tratamento de Queimados do Hospital Universitário de Londrina. Foram estudadas 46 crianças (1 a 12 anos), apresentando entre 15% e 45% de Superfície Corporal Queimada, admitidas até a 12a hora após o acidente. Intervenção: Para a ressuscitação hídrica dos pacientes, foi utilizada solução cristaloide baseada na Fórmula de Parkland modificada, ajustada de acordo com o débito urinário. O Grupo Intervenção (23 pacientes) foi randomizado para receber solução de albumina entre 8 e 12 horas do acidente, e o Grupo Controle (23 pacientes) recebeu a mesma solução após 24 horas do acidente. Resultados: Houve possibilidade de redução de infusão de solução cristaloide durante o período de ressuscitação dos pacientes. O grupo Intervenção recebeu um volume de solução cristaloide com uma mediana de -31,99% (P=0,025) no 1º dia, -19,37% (P=0,002) no 2º dia e -45,3% (P=0,002) no 3º dia de ressuscitação. Não foram observadas diferenças significantes entre os grupos em relação à diurese. A incidência acumulada de fluid creep na população...
Introduction: The fluidic resuscitation of burned children is a challenge due to intolerance to insufficient or excessive supply of liquids. There are questions regarding the use of colloids in fluid resuscitation solution as well as the timing of the administration. The ideal time for the administration of albumin, in other words whether it should be used as a rescue strategy when the volume of crystalloid infused becomes excessive, or routinely, as primary intervention for patients with extensive burns, remains in the focus of discussion. Objectives: Evaluate the clinical outcomes of children with burn injuries who have received early infusion treatment (between 8 and 12 hours after the accident) with natural colloids solution compared to the children who have received late infusion treatment (24 hours after the accident) with the same solution, in order to resuscitate them in the acute phase. Methods: Randomized Controlled Trial carried out at the Burn Treatment Center, State University of Londrina. Forty-six children (1 to 12 year olds) who had between 15% and 45% total body surface area and were admitted up to 12 hours after the accident were studied. Intervention: For the fluid resuscitation of patients, a crystalloid solution based on modified Parkland Formula was adjusted according to urine output. The Intervention Group (n= 23) were randomized to receive albumin solution between 8 and 12 hours after the accident and the Control Group (n= 23) received the same solution later than 24 hours after the burn injury. Results: During the resuscitation of patients, it was possible to reduce the infusion of crystalloid solution. The Intervention Group required a volume of crystalloid solution with a median of -31.99% (P = 0.025) on day 1, - 19.37% (P = 0.002) on day 2 and -45.3% (P = 0.002) on day 3 of resuscitation. No significant differences were observed in the groups in relation to diuresis. The cumulative incidence of fluid creep in the population...
Subject(s)
Humans , Infant , Child, Preschool , Child , Albumins/therapeutic use , Pediatrics , Burns/therapy , Resuscitation , Receptors, Albumin/therapeutic use , Early Medical Intervention/methodsABSTRACT
PURPOSE: To determine the optimum culture conditions by investigating isolated rat hepatocytes cultured in medium containing different glucose concentrations. METHODS: Hepatocytes were isolated from rats using a two-step perfusion technique and divided into the following two groups cultured in medium containing different glucose concentrations: (1) low-glucose group and (2) high-glucose group. Total cell count and viability of cultured rat hepatocytes and liver function parameters (i.e., concentrations of albumin, ammonia, and urea in the culture medium) were measured. The morphology of cultured rat hepatocytes was examined by staining with hematoxylin and eosin, and albumin receptor expression was confirmed by immunofluorescence. RESULTS: Total cell count and viability showed smaller increases in the low-glucose group than the high-glucose group, although the difference was not statistically significant (P = 0.112 and P = 0.147, respectively). The levels of albumin (P = 0.943), ammonia (P = 0.744), and urea (P = 0.709) were not significantly different between the two groups. In both groups, the function of cultured hepatocytes decreased significantly over time. The morphology of hepatocytes was well maintained in both groups at 3 days. On day 7, the cytoplasm was transformed into a spindle shape. On day 10, these changes were exaggerated, and were more prominent in the high-glucose group. CONCLUSION: Morphological assessment indicated that low-glucose culture medium is better than high-glucose culture medium for culturing of hepatocytes, although there was not significantly different in functional assessment. The cultured hepatocytes with low-glucose culture medium could be maintained for 7 days.
Subject(s)
Animals , Rats , Ammonia , Cell Count , Cell Transplantation , Cytoplasm , Eosine Yellowish-(YS) , Fluorescent Antibody Technique , Glucose , Hematoxylin , Hepatocytes , Liver , Perfusion , Receptors, Albumin , UreaABSTRACT
<p><b>OBJECTIVE</b>To observe and compare the effects of Hanfangji Compound and IFN-gamma on expressions of transthyretin (TTR) , inter-alpha inhibitor H1 (ITIH1) and serpin peptidase inhibitor clade F member 2 (SERPINF2) of hepatic stellate cells (HSC-T6).</p><p><b>METHOD</b>Hanfangji Compound and IFN-gammaof different concentrations were used in hepatic stellate cell-T6 (HSC-T6) for 48 h. Flow cytometer was used to detect the effects of Hanfangji Compound and IFN-gamma on HSC proliferation. RT-PCR method was adopted to detect mRNA expressions of TFR, ITIH1 and SERPINF2. TTR, ITIH1 and SERPINF2 secretions were detected by ELISA. The protein localizations of TTR, ITIH1 and SERPINF2 were examined by immune fluorescence. The protein expression of TfR and ITIHI were determined by Western blot.</p><p><b>RESULT</b>After Hanfangji Compound and IFN-gamma were adopted in HSC-T6, compared with the control group, the cell proliferation was inhibited obviously (P < 0. 05) , protein expressions of TTR, ITIH1 and SERPINF2 and mRNA expression increased significantly, with certain correlation with concentrations of Hanfangji Compound. The 2. 5 g L-I Hanfangji Compound group was superior to the IFN-gamma group (P <0. 05).</p><p><b>CONCLUSION</b>Hanfangji Compound can inhibit HSC proliferation, upregulated TTR, ITIH1 and SERPINF2 proteins and mRNA expression, which may be one of mechanisms of anti-hepatic fibrosis of Hanfangji Compound.</p>
Subject(s)
Humans , Alpha-Globulins , Genetics , Metabolism , Blotting, Western , Cell Line, Tumor , Cell Proliferation , Drugs, Chinese Herbal , Pharmacology , Enzyme-Linked Immunosorbent Assay , Hepatic Stellate Cells , Cell Biology , Metabolism , Receptors, Albumin , Genetics , Metabolism , alpha-2-Antiplasmin , Genetics , MetabolismABSTRACT
La prevalencia de desnutrición en el paciente oncológico es un problema común; se estima que la incidencia de malnutrición oscila entre 15 y 40% al momento del diagnóstico del cáncer, y aumenta hasta 80% en casos de enfermedad avanzada. Las consecuencias de la desnutrición implicarán la aparición de mayor número de complicaciones en el período postquirúrgico, estancias hospitalarias prolongadas, mayor coste económico, y una mortalidad más elevada. Se estudió la proporción de pacientes con desnutrición que concurren al servicio de Nutrición y Diabetes para Valoración del Estado Nutricional pre-quirúrgico en el Hospital Municipal de Oncología Marie Curie, de junio a noviembre de 2008. La detección se realizó por el método propuesto por Ulibarri y col (dentro del sistema CONUT). Fueron evaluados 132 pacientes. Un 7,2% (n=9) de la muestra presentó una pérdida de peso mayor al 10% en los últimos 6 meses (IC95%2,2-12,2%), la pérdida del 5 al 10% del peso corporal se presentó en el 12,9% (n=16) (IC95% 6,6-19,2%), predominando la pérdida de menos del 0 - 5% del peso corporal con un 79,8% (n=99) (IC 95% 72,3-87,3%). El 97,7% de la muestra presentó valores de albúmina mayores a 3,5 g/dl (IC 95% 94,8-100%); todos los evaluados presentaron valores de de proteínas totales mayor a 5 g/l; el 18,2% (IC95% 11,2-25,4%) presentó valores de colesterol menores a 180 mg/dl; y un 63,6% presentó valores inferiores a 1600 linfocitos/mm3 (IC 95% 55,0-72,2%). La prevalencia de desnutrición resultó del 47,7% (IC95% 38,8-56,6%). La desnutrición aparece como un rasgo importante en la población estudiada, y, considerando las implicaciones de dicha situación, deberían tomarse medidas para revertir la situación en pos de lograr que los pacientes oncológicos lleguen a la cirugía bien nutridos.
Subject(s)
Lymphocyte Count , Malnutrition , Neoplasms , Receptors, AlbuminABSTRACT
En esta presentación se hace un recuento histórico de la aplicación de terapias intravenosas para reanimación básica en campos de batalla y epidemias por causa infecciosa. Se analizan las propiedades reológicas y efectos vasculares, renales y hepáticos de las diferentes sustancias cristaloides, coloides, dextranos y poligelatinas. Aunque la aplicación, el tiempo, la dosis y la patología básica son controversiales el autor indica una serie de razones basadas en las recomendaciones del consenso en 1995 y diversos grados de evidencia, para inclinarse por el uso de coloides con las excepciones debidas a cada caso en particular...
Subject(s)
Blood Coagulation , Colloids , Dextrans , Polygeline , Receptors, Albumin , Tissue Expansion Devices , ViscosityABSTRACT
<p><b>BACKGROUND</b>To investigate the relationship between interleukin-6 (IL-6) levels in serum and HBV replication and the role of IL-6 in liver damage in chronic hepatitis B patients.</p><p><b>METHODS</b>Detected IL-6 levels and polymerized human serum albumin receptor (PHSA-R) in serum of different type of HBV markers positive patients and of different degree of chronic hepatitis B patients.</p><p><b>RESULTS</b>The results showed that the serum IL-6 levels were increased significantly in PHSA-R positive patients than in PHSA-R negative patients in chronic hepatitis B (P<0.01). The serum IL-6 levels were correlated with the levels of PHSA-R (r=0.694, P<0.01), and the degree of symptoms.</p><p><b>CONCLUSIONS</b>This study suggested that the IL-6 levels in serum of hepatitis B patients correlated HBV replication and the degree of liver damage, serum IL-6 levels may be used as a value indicating of HBV replication, the degree of symptoms and the effect of treatment.</p>
Subject(s)
Adult , Aged , Humans , Middle Aged , Hepatitis B virus , Physiology , Hepatitis B, Chronic , Blood , Interleukin-6 , Blood , Receptors, Albumin , Blood , Virus ReplicationABSTRACT
The association of hyperthyroxinemia and euthyroidism is frequent and characterized by high plasma thyroxin concentrations, normal TSH values and absence of clinical signs of hyperthyroidism. We report an asymptomatic 28 years old male presenting with a serum total plasma thyroxin of 18.5 µg/dl (N 6.1-12.5), a free thyroxin of 2.9 ng/dl (N 0.8-1.4), a TSH of 3.4 µIU/ml (N 0.5-5), and a triiodothyronine of 128 ng/dl (N 80-180). Laboratory assessment did not find high thyroxin binding globulin, albumin or prealbumin concentrations or antithyroxin antibodies. The thyroxin binding capacity of albumin was elevated to 58.2 µg/dl (N 11.5-34.1). TSH responded normally to TRH stimulus and was suppressed with exogenous triiodothyronine, which caused an hyperthyroid syndrome. We concluded that this patient had a familial dysalbuminemia