Detection of Diarrheagenic Escherichia coli in Bovine Meat in the Northern Region of Paraná State, Brazil

Abstract Ground bovine meat is commonly consumed by the population of Brazil. However, it constitutes an excellent medium for the multiplication of microorganisms due to available nutrients and handling practices prior to consumption. Here, we examined 100 samples of ground beef for the presence of diarrheagenic Escherichia coli (DEC) pathotypes by PCR, and characterized isolates by analyzing their adherence to HEp-2 cells, serotype, antimicrobial susceptibility, and phylogeny. Enteroaggregative E. coli was detected in five (5%) meat samples, Shiga toxin-producing E. coli in three (3%), and atypical enteropathogenic E. coli in two (2%). According to the phylogeny, six isolates (60%) were classified in group A, two (20%) in group B1, and two (20%) in group E. The detected serotypes were O3:H2, O93:H9, O93:H46, O105ab:H7, O152:H8, O156:H10, and O175:H7. The antimicrobial susceptibility testing showed that one sample (10%) was resistant to ampicillin, two (20%) to sulfamethoxazole-trimethoprim, and two (20%) to cephalothin. Based on these results, bovine ground meat for human consumption can serve as a reservoir of DEC, which emphasizes the importance of appropriate hygienic-sanitary conditions during handling at every stage from slaughter to table.

Salmonella: sorotipos identificados das cepas isoladas de pacientes hospitalizados e näo hospitalizados, na Regiäo de Presidente Prudente, SP, no periódo de 1978-1997 / Sorotypes idnetified of strains isolated from hospitalized non-hospitalized patients, in Presidente Prudente area, SP, during the period of 1978-1997

No período de 1978-1997, foram identificadas 413 cepas de Salmonella, isoladas de coproculturas de origem ambulatorial e hospitalar, no Setor de Microbiologia do Laboratório I Regional de Presidente Prudente. Determinou-se a sensibilidade aos agentes antimicrobianos de 394 cepas de Samonella. Entre as cepas isoladas de coproculturas de origem ambulatorial observou-se grande diversidade de sorotipos (27 sorotipos) com predominância de S. Enteritidis 24,4(por cento), S. Infantis 19,3(por cento) e S. Agona 10,1(por cento). Em relaçäo às cepas isoladas de pacientes hospitalizados, foram identificados 8 diferentes sorotipos, sendo que a S. Typhimurium representou o sorotipo prevalente 95,6(por cento), seguido de S. Typhi 1(por cento) e S. I4,12:i:-1(por cento). Com relaçäo à sensibilidade aos agentes antimicrobianos, as cepas de origem hospitalar apresentam multirresistência, enquanto que aquelas de origem ambulatorial foram sensíveis à maioria dos antimicrobianos utilizados