ABSTRACT
Abstract Purpose: To evaluate the feasibility of an experimental model of autologous fat graft (AFG) in different interstitial pressure (IP) environments. Methods: Three mini-pigs(Minipig-BR) with age of 8 months (weight: 25-30 kg) were used. AFG were collected from the bucal fat pad, and grafted in the intramuscular pocket (biceps femoralis muscle). IP model was based on a fusiform ressection followed by primary closure "under tension". A blood pressure catheter located in the intramuscular region connected to a pressure module was applied to quantify IP. Results: The mean operative time was 236 min (210 - 272 min). All the AFG and muscular segments were removed successfully. Average interstitial pressure CP and H were 3 and 10.6 mmHg respectively. The AFG were biopsied for histopathological analysis 30 days after graft. Hematoxylin-eosin staining and immunohistochemical analyzes (TNF-alpha, CD31 and Perilipine with monoclonal antibodies) were employed. Conclusion: The data show that minipigs model could be used as a recipient site for autologous fat graft techniques and allow the development of studies to explore the AFG intake and pathophysiology response.
Subject(s)
Animals , Male , Transplantation, Autologous/methods , Adipose Tissue/transplantation , Plastic Surgery Procedures/methods , Disease Models, Animal , Pressure , Swine , Swine, Miniature , Transplantation, Autologous/standards , Immunohistochemistry , Feasibility Studies , Tumor Necrosis Factor-alpha , Platelet Endothelial Cell Adhesion Molecule-1/analysis , Plastic Surgery Procedures/standards , Perilipins/analysis , Graft SurvivalSubject(s)
Humans , Child , Adolescent , Adult , Cyclophosphamide/administration & dosage , Cyclophosphamide/therapeutic use , Nervous System Neoplasms/epidemiology , Nervous System Neoplasms/mortality , Nervous System Neoplasms/pathology , Nervous System Neoplasms/physiopathology , Nervous System Neoplasms/therapy , Transplantation, Autologous/statistics & numerical data , Transplantation, Autologous/standards , Transplantation, Autologous/pathology , Glioblastoma/physiopathology , Glioblastoma/therapy , Medulloblastoma/physiopathology , Medulloblastoma/therapyABSTRACT
En el período comprendido entre octubre de 1988 y abril de 1990, fueron tratados en nuestra unidad 24 pacientes con diagnóstico de quemaduras por electricidad, representando el 34,28// de las internaciones. Fueron seleccionados 4 casos demostrativos con lesiones graves, en los cuales se emplearon técnicas reparadoras como expansión tisular, colgajos cutáneos, colgajos musculares pediculados y libres con transferencia microquirúrgica