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1.
Medicentro (Villa Clara) ; 27(4)dic. 2023.
Article in Spanish | LILACS | ID: biblio-1534854

ABSTRACT

El concepto de biopelículas ha surgido de forma paulatina durante un largo período; se presentan como estructuras tridimensionales compuestas por células sésiles de microorganismos que crecen y se adhieren irreversiblemente a superficies, tanto vivas como inertes. Su capacidad de desarrollarse, tanto en superficies bióticas como abióticas, es una característica que los relaciona directamente con la salud humana. Distintas infecciones óticas se han inculpado a la presencia de biopelículas en las mucosas como en la otitis media con efusión, de igual forma se manifiestan en la aparición y persistencia de la otitis media crónica. Las biopelículas afines con otitis media, generalmente, contienen uno o múltiples especies de bacterias otopatógenas primarias. La comprensión de la biopelicula auxiliará el progreso de nuevas terapias y estrategias de control, al evitar enfermedades infecciosas ya que las bacterias formadoras de biopelículas son una seria amenaza para la salud pública debido a su alta resistencia a los antimicrobianos.


The concept of biofilms has emerged gradually over a long period; they appear as three-dimensional structures composed of sessile cells of microorganisms that grow and adhere irreversibly to surfaces, both living and inert. Their ability to develop, both on biotic and abiotic surfaces, is a characteristic that directly relates them to human health. Different ear infections have been blamed on the presence of biofilms on the mucous membranes, such as otitis media with effusion, in the same way they manifest themselves in the appearance and persistence of chronic otitis media. Otitis media-related biofilms generally contain one or multiple species of primary otopathogenic bacteria. The understanding of the biofilm will help us refine new therapies and control strategies, by avoiding infectious diseases since biofilm-forming bacteria are a serious threat to public health due to their high resistance to antimicrobials.


Subject(s)
Biofilms , Otitis Media, Suppurative , Ear
2.
Medicentro (Villa Clara) ; 27(3)sept. 2023.
Article in Spanish | LILACS | ID: biblio-1514483

ABSTRACT

Introducción: el estado de salud de los tejidos periimplantarios es de vital importancia en el éxito de la rehabilitación implantosoportada, por esta razón, es necesario observar todos aquellos factores que contribuyen a mantener este estado y dentro de ellos, principalmente: la higiene bucal. Objetivo: determinar la influencia de la higiene bucal en el estado de salud de los tejidos periimplantarios. Métodos: se realizó un estudio descriptivo, observacional y transversal en el servicio de Prótesis de la Facultad de Estomatología de Villa Clara, en el período comprendido entre los años 2017 y 2019. El universo de estudio estuvo constituido por 45 pacientes portadores de rehabilitaciones implantosoportadas; las unidades de análisis fueron los implantes y los tejidos que rodean a las 85 prótesis fijas realizadas a dichos pacientes que cumplieron con los criterios de inclusión. Se emplearon la observación clínica y radiográfica, y se elaboró un formulario como instrumento. Se evaluó la higiene bucal y el estado de los tejidos periimplantarios como principales variables. La información obtenida se recopiló en una base de datos, se procesó y se sometió a pruebas de independencia (el estadígrafo Ji cuadrado y su posibilidad asociada) para mostrar la relación entre las variables. Resultados: las variables analizadas evidenciaron una relación significativa de la higiene bucal con el estado de salud de los tejidos periimplantarios a favor de la buena higiene y los tejidos sanos. Conclusiones: la buena higiene bucal evidenciada contribuyó a que los tejidos periimplantarios se mantuvieran sanos.


Introduction: peri-implant tissue health state is of vital importance in the success of implant-supported rehabilitation; for this reason, it is necessary to observe all those factors that contribute to maintaining this state, mainly oral hygiene. Objective: to determine the influence of oral hygiene on peri-implant tissue health status. Methods: a descriptive, observational and cross-sectional study was carried out in the Prosthesis service at the Dental Faculty of Villa Clara between 2017 and 2019. The universe of study consisted of 45 patients with implant-supported rehabilitations; the units of analysis were the implants and the tissues surrounding the 85 fixed prostheses performed on those patients who met the inclusion criteria. Clinical and radiographic observations were used, and a form was developed as an instrument. Oral hygiene and peri-implant tissue state were evaluated as the main variables. The information obtained was compiled in a database as well as processed and subjected to independence tests (the Chi-square statistic and its associated possibility) to show the relationship among the variables. Results: the analyzed variables showed a significant relationship between oral hygiene and the peri-implant tissue health status in favour of good hygiene and healthy tissues. Conclusions: the evidenced good oral hygiene contributed to the maintenance of healthy peri-implant tissues.


Subject(s)
Rehabilitation , Dental Implants , Biofilms , Microbiota
3.
Biomédica (Bogotá) ; 43(Supl. 1): 77-88, ago. 2023. tab, graf
Article in Spanish | LILACS | ID: biblio-1533901

ABSTRACT

Introducción. El 65 % de las infecciones humanas son producidas por bacterias o levaduras, cuya capacidad de formar biopelículas las hace más resistentes a los antimicrobianos y antifúngicos. Objetivo. Determinar la capacidad de formación de biopelículas en aislamientos bacterianos y fúngicos por medio de los métodos cuantitativo de microtitulación con cristal violeta y cualitativo de cultivo en agar con rojo Congo. Materiales y métodos. Con el método cuantitativo, se utilizaron los medios de cultivo infusión cerebro-corazón, tripticasa de soya y Müeller-Hinton para aislamientos bacterianos; para levaduras, se usaron caldo infusión cerebro-corazón y Sabouraud dextrosa. Para el método cualitativo de cultivo en agar, se utilizaron los mismos medios de cultivo más una solución con 3 % de rojo Congo y 10 % de dextrosa. Cómo método de referencia, se utilizó la propuesta de Stepanovic et al. Resultados. Se evaluaron 103 aislamientos bacterianos y 108 de levaduras. No es recomendable sustituir el caldo infusión cerebro-corazón por los caldos tripticasa de soya y Müeller-Hinton en el método cuantitativo, para evaluar la formación de biopelículas en los aislamientos bacterianos. El medio Sabouraud dextrosa, en caldo y agar, puede sustituir al de infusión de cerebro-corazón para evaluar la formación de biopelículas en levaduras, tanto por el método cuantitativo como por el cualitativo. Conclusión. El estudio de las biopelículas en el laboratorio de microbiología, a partir del método cualitativo de cultivo en agar con rojo Congo, es un procedimiento sencillo, rápido y de bajo costo, que proporciona información útil para el diagnóstico y la terapéutica de infecciones persistentes causadas por bacterias y levaduras.


Introduction. Sixty-five percent of human infections are caused by bacteria or yeasts able to form biofilms. This feature makes them more resistant to antimicrobials and antifungals. Objective. To determine biofilm formation capacity of bacterial and fungal isolates by quantitative crystal violet microtiter and qualitative Congo red agar methods. Materials and methods. Brain-heart infusion, trypticase soy broth and Müeller-Hinton culture media were used in bacterial isolates for the quantitative method; brain-heart infusion broth and Sabouraud dextrose were used for yeasts. The same culture media plus 3% Congo red and 10% dextrose were used to apply the qualitative method in agar. The proposal by Stepanovic, et al. was used as a reference method. Results. We evaluated 103 bacterial isolates and 108 yeasts isolates. We did not recommend substitute brain-heart infusion broth for trypticase soy and Müeller-Hinton broths for biofilm formation assessment in bacterial isolates using the quantitative method. Sabouraud dextrose medium, both broth and agar, can replace brain-heart infusion to assess biofilm formation in yeasts, quantitatively and qualitatively. Conclusion. The study of biofilms in the microbiology laboratory, using Congo red agar qualitative method, is a simple, fast, and inexpensive procedure that provides precise information for the diagnosis and treatment of persistent infections caused by bacteria and yeasts.


Subject(s)
Gram-Negative Bacteria , Gram-Positive Bacteria , Yeasts , Biofilms , Congo Red
4.
Online braz. j. nurs. (Online) ; 22(supl.1): e20236609, 03 fev 2023. ilus
Article in English, Portuguese | LILACS, BDENF | ID: biblio-1414190

ABSTRACT

OBJETIVO: Analisar a efetividade de Polihexametileno Biguanida (PHMB), comparado à solução salina na carga microbiana de pacientes com feridas. MÉTODO: Protocolo de revisão sistemática, construído segundo o Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA), de acordo com metodologia do Joanna Briggs Institute (JBI). Os estudos serão avaliados por dois pesquisadores independentes, nas bases de dados: Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS), Base de Dados de Enfermagem (BDENF), Sistema Online de Busca e Análise de Literatura Médica (MEDLINE)e Excerpta Medica Database (Embase). As pesquisas a serem incluídas serão aquelas publicadas em português, inglês ou espanhol e a busca não definirá recorte temporal. Serão desconsiderados estudos em animais ou in vitro, revisões, cartas ao editor ou estudos de casos. Após a seleção dos estudos, a extração de dados ocorrerá de maneira sistemática e os registros correspondentes serão feitos de forma narrativa e tabular.


OBJECTIVE: To analyze the effectiveness of polyhexamethylene biguanide (PHMB) compared to saline on the microbial load of wounds. METHOD: Systematic review protocol, built according to the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) and the Joanna Briggs Institute's (JBI) methodology. Studies will be evaluated by two independent researchers in the following databases: Latin America and the Caribbean Literature on Health Sciences (LILACS), Nursing Database (BDENF), Medical Literature Analysis and Retrieval System Online (MEDLINE), and Excerpta Medica Database (Embase). Studies published in Portuguese, English, or Spanish will be included, and the search will not be restricted by publication date. Animal or in vitro studies, reviews, letters to the editor, and case studies will be excluded. After selecting studies, data extraction will take place systematically, and the corresponding records will be presented in a narrative and tabular way.


Subject(s)
Humans , Adult , Aged , Wound Healing , Wound Infection , Wounds and Injuries , Biguanides , Bacterial Load , Saline Solution , Biofilms
5.
Braz. J. Pharm. Sci. (Online) ; 59: e20412, 2023. tab, graf
Article in English | LILACS | ID: biblio-1439492

ABSTRACT

Abstract Treatment with plant is considered an effective option against increased antibiotic resistance. In this study antibiofilm activity of methanol (CH3OH), chloroform (CHCl3), ethyl acetate (EtOAc) and water (H2O) extracts of Hypericum atomarium Boiss. which is member of Hypericum genus was evaluated in Pseudomonas aeruginosa PAO1 and antibacterial performance against Gram (+) and Gram (-) strains and also bioactive compounds of extract were analysed using by HPLC and GC-MS. According to antibacterial activity test results the extracts were effective all Gram (+) bacteria and Gram (-) Chromobacterium violaceum (MICs ranging from 0.42 µg/ml to 4.3 mg). Inhibition effect of biofilm formation was found to be different rate in extracts (methanol-63%, chloroform-52%). The major flavonoids were detected (−)-epicatechin (2388.93 µg/ml) and (+)-catechin (788.94 µg/ml). The main phenolic acids were appeared as caffeic acid 277.34 µg/ml and chlorogenic acid 261.79 µg/ml. And according to GC results α-pinene was found main compound for three solvent extracts methanol, chloroform and ethyl acetate 67.05, 62.69, 49.28% rate respectively


Subject(s)
Plants/metabolism , In Vitro Techniques/methods , Biofilms/classification , Hypericum/classification , Sprains and Strains/complications , Chromatography, High Pressure Liquid/methods , Chromobacterium/isolation & purification , Acetates/classification
6.
São José dos Campos; s.n; 2023. 60 p. ilus, tab.
Thesis in Portuguese | LILACS, BBO | ID: biblio-1428671

ABSTRACT

Os microrganismos resistentes a diferentes classes de agentes antimicrobianos têm se tornado cada vez mais comuns e atualmente são denominados como multirresistentes. Nos hospitais, tais microrganismos apresentam maior perigo, pois são causadores de infecções nosocomiais e a higienização bucal deficiente dos pacientes internados pode tornar a cavidade bucal um sítio para proliferação desses microrganismos multirresistentes. Diante do exposto, novos compostos com ação antimicrobiana precisam ser estudados. O objetivo deste estudo foi avaliar quimicamente o extrato hidroalcóolico de própolis verde de Baccharis dracunculifolia e de Cinnamomum verum (canela) que foram obtidos a partir da extração da matériaprima, analisar a atividade antimicrobiana e antibiofilme dos extratos isolados e combinados contra quatro cepas clínicas multirresistentes de Pseudomonas aeruginosa e Acinetobacter baumannii e verificar a citotoxicidade dos produtos vegetais in vitro em linhagem celular de queratinócitos humanos (HaCat). Para tanto, os extratos vegetais foram preparados a partir da matéria-prima da canela em casca e da própolis bruta. Em seguida, foram caracterizados quimicamente por cromatografia líquida de alta eficiência (HPLC-DAD) para identificação dos principais compostos e a análise do teor de sólidos solúveis dos extratos vegetais também foi realizada. Para avaliação antimicrobiana, foram performados o teste de microdiluição em caldo de acordo com a Clinical and Laboratory Standards Institute (CLSI) e a análise de Checkerboard, para avaliar o efeito combinado dos extratos. A atividade antibiofilme dos extratos combinados foi realizada por meio do teste de MTT, no qual diferentes tempos de contato (5 e 30 min) e diferentes modalidades (inibição na formação do biofilme bacteriano e erradicação do biofilme bacteriano já formado) foram testadas. Para ação citotóxica, as células foram cultivadas em meio DMEM e semeadas na placa de 96 poços. Após aderência inicial, aplicou-se os extratos em diferentes concentrações baseadas nas análises microbiológicas para avaliação da viabilidade celular por meio do teste de MTT. Os dados foram analisados por ANOVA e teste de Tukey, ou Kruskal-Wallis e Dunn, considerando um nível de significância de 5%. Os compostos identificados no extrato de própolis verde de B. dracunculifolia foram ácido clorogênico, derivado do ácido cinâmico e apigenina. O aldeído cinâmico foi o principal composto identificado no extrato de C. verum. Os extratos vegetais apresentaram ação bactericida sobre todas as cepas analisadas e, quando combinados, os extratos atuaram de modo aditivo e algumas combinações sinérgicas foram encontradas. O protocolo de inibição da formação do biofilme promoveu percentuais de redução superiores quando comparado ao protocolo de erradicação. Valores expressivos de 83,86% (p < 0,05) de inibição da formação de biofilme de uma cepa clínica de A. baumannii e 89,31% (p < 0,05) de inibição em uma cepa clínica de P. aeruginosa foram encontrados com a aplicação dos extratos combinados. A atuação dos produtos vegetais foi estatisticamente semelhante a atuação da clorexidina 0,12%. Em conclusão, os extratos de própolis verde e canela na forma isolada ou combinada apresentaram ação antimicrobiana e antibiofilme sobre cepas clínicas de A. baumannii e P. aeruginosa multirresistentes. Dessa forma, os produtos vegetais são promissores agentes antissépticos para futuras formulações odontológicas. (AU)


Microorganisms resistant to different classes of antimicrobial agents have become increasingly common and are currently called multidrug resistant. In hospitals, such microorganisms are more dangerous, as they cause nosocomial infections and poor oral hygiene in hospitalized patients can make the oral cavity a site for the proliferation of these multiresistant microorganisms. Given the above, new compounds with antimicrobial action need to be studied. The objective of this study was to chemically evaluate the hydroalcoholic extract of green propolis from Baccharis dracunculifolia and Cinnamomum verum (cinnamon) that were obtained from the extraction of the raw material, to analyze the antimicrobial and antibiofilm activity of the isolated and combined extracts against four clinical strains multiresistant strains of Pseudomonas aeruginosa and Acinetobacter baumannii and verify the cytotoxicity of plant products in vitro in human keratinocyte cell lineage (HaCat). For this purpose, plant extracts were prepared from raw cinnamon bark and raw propolis. Then, they were chemically characterized by high performance liquid chromatography (HPLC-DAD) to identify the main compounds and the analysis of the soluble solids content of the plant extracts was also performed. For antimicrobial evaluation, the broth microdilution test according to the Clinical and Laboratory Standards Institute (CLSI) and the Checkerboard analysis were performed to evaluate the combined effect of the extracts. The antibiofilm activity of the combined extracts was performed using the MTT test, in which different contact times (5 and 30 min) and different modalities (inhibition of bacterial biofilm formation and eradication of already formed bacterial biofilms) were tested. For cytotoxic action, cells were cultured in DMEM medium and seeded in the 96-well plate. After initial adhesion, the extracts were applied at different concentrations based on microbiological analyzes to assess cell viability through the MTT test. Data were analyzed by ANOVA and Tukey's test, or Kruskal-Wallis and Dunn, considering a significance level of 5%. The compounds identified in the green propolis extract of B. dracunculifolia were chlorogenic acid, cinnamic acid derivative and apigenin. Cinnamic aldehyde was the main compound identified in the C. verum extract. The plant extracts showed bactericidal action on all strains analyzed and, when combined, the extracts acted additively and some synergistic combinations were found. The biofilm formation inhibition protocol promoted higher reduction percentages when compared to the eradication protocol. Significant values of 83.86% (p < 0.05) inhibition of biofilm formation in a clinical strain of A. baumannii and 89.31% (p < 0.05) inhibition in a clinical strain of P. aeruginosa were found with the application of the combined extracts. The performance of plant products was statistically similar to the performance of 0.12% chlorhexidine. In conclusion, extracts of green propolis and cinnamon, in isolated or combined form, showed antimicrobial and antibiofilm action on multiresistant clinical strains of A. baumannii and P. aeruginosa. Thus, plant products are promising antiseptic agents for future dental formulations. (AU)


Subject(s)
Propolis , Pseudomonas aeruginosa , Biofilms , Cinnamomum , Acinetobacter baumannii
7.
Rev. odontopediatr. latinoam ; 13: 223548, 2023. ilus
Article in Spanish | LILACS, COLNAL | ID: biblio-1435309

ABSTRACT

La mínima intervención ha ido ganando cada vez más notoriedad en la Odontología, reflejando una creciente demanda por parte de los profesionales que buscan técnicas que promuevan tratamientos eficientes y que preserven la estructura dentaria. Objetivo: El presente estudio tuvo como objetivo promover el control de caries no restaurador (CCNR) en un paciente infantil, mediante una regularización de la cavidad de la lesión cariosa para permitir una mejor limpieza del área, junto con un protocolo complementario de fluoración. Presentación de caso: Paciente masculino de 4 años de edad con higiene bucal satisfactoria, sin embargo con acumulación de biopelícula entre los incisivos centrales superiores y sin síntomas ni quejas estéticas. Se alisaron las caras mesiales de los incisivos centrales con tiras abrasivas para eliminar zonas socavadas y facilitar el acceso para mejorar la higiene bucal, y se aplicó el barniz de flúor en 4 sesiones, con un intervalo de una semana entre cada aplicación. La madre de la paciente fue instruida sobre cuidados de higiene y alimentación. Durante 36 meses se realizó seguimiento clínico y radiográfico del paciente con retornos cada 12 meses. También se reevaluaron el índice de placa visible y los resultados de la guía de higiene y dieta. Conclusión; En este caso clínico, la fluorterapia asociada a la regularización superficial demostró ser una alternativa de tratamiento exitosa a largo plazo, permitiendo la preservación del tejido dentario y previniendo la progresión de las lesiones cariosas. El concepto ultraconservador puede ser una alternativa válida por su eficacia, sencillez, bajo coste y buena aceptación por parte de los niños y sus padres


A mínima intervenção vem ganhando cada vez mais notoriedade na Odontologia, refletindo uma crescente demanda de profissionais que buscam por técnicas que promovam tratamentos eficientes e preservem a estrutura dental. Objetivo: O presente estudo teve como objetivo promover um controle cavitário não restaurador (CCNR) em um paciente infantil, por meio de uma regularização da cavidade da lesão de cárie para permitir uma melhor higienização da área, associado a um protocolo complementar de flúor. Caso clinico: Paciente do sexo masculino, 4 anos de idade, com higiene bucal regular, entretanto com acúmulo de biofilme entre os incisivos centrais superiores e sem sintomatologia ou queixa estética. As superfícies mesiais dos incisivos centrais foram regularizadas com tiras abrasivas para remover áreas retentivas e facilitar o acesso para melhorar a higiene bucal. O verniz fluoretado foi aplicado em 4 sessões, com intervalo de uma semana entre cada aplicação. A mãe do paciente foi orientada quanto à higiene e cuidados com a dieta. Durante 36 meses, houve o acompanhamento clínico e radiográfico do paciente com retornos a cada 12 meses. O índice de placa visível e os resultados da orientação de higiene e dieta também foram reavaliados. Conclusão: Neste caso clínico, a regularização de superfícies associada a fluorterapia, mostrou-se uma alternativa de tratamento de sucesso a longo prazo, permitindo a preservação do tecido dentário e impedindo a progressão das lesões de cárie. O conceito ultraconservador pode ser uma alternativa válida devido à sua eficácia, simplicidade, baixo custo e boa aceitação pelas crianças e seus pais.


The minimal intervention has been gaining more notoriety in Dentistry, reflecting growing demand from professionals looking for techniques that promote efficient treatments and preserve the dental structure. Objective: The present study aimed to promote non-restorative cavity control (CCNR) in a child patient through regularization of the cavity of the carious lesion to allow better cleaning of the area, together with a complimentary fluoride protocol. Case presentation: Male patient, 4 years old, presenting adequate oral hygiene. However, biofilm accumulation occurs between the upper central incisors without symptoms or aesthetic complaints. Therefore, the mesial surfaces of the central incisors were smoothed with abrasive strips to remove undercut areas and facilitate access to improve oral hygiene. The fluoride varnish was applied in 4 sessions, with an interval of one week between each application. The patient's mother was instructed on hygiene and diet care. During 36 months, there was a clinical and radiographic follow-up of the patient with returns every 12 months. The visible plaque index and the hygiene and diet guidance results were also re-evaluated. Conclusion: In this clinical case, surface regularization associated fluortherapy proved to be a successful long-term treatment alternative, allowing the preservation of dental tissue and preventing the progression of caries lesions. The ultra-conservative concept can be a valid alternative due to its effectiveness, simplicity, low cost, and good acceptance by children and their parents.


Subject(s)
Humans , Male , Child, Preschool , Dental Caries , Fluorine , Oral Hygiene , Biofilms , Halogenation
8.
Braz. dent. sci ; 26(2): 1-8, 2023. ilus, tab
Article in English | LILACS, BBO | ID: biblio-1426430

ABSTRACT

Objective: in this study, biofilm formation by Candida albicans in fixed orthodontic appliances was evaluated. Material and Methods: a total of 300 conventional metal brackets (MC), ceramic (CB), self-ligation (SLB), nickel-titanium (NiTi), and nickel-chromium (NiCr) wires, and ligatures types were organized into thirty groups (n=10). To induce biofilm formation, brackets, wires, and ligatures were joined, sterilized, placed in 24-well plates, contaminated with standardized suspensions of C. albicans (107 cells/mL), and incubated at 37 °C for 48 h with shaking. The biofilms formed were detached using an ultrasonic homogenizer, and suspensions were serially diluted and plated on Sabouraud dextrose agar to determine colony-forming units per mL. Scanning electron microscopy was performed before and after the biofilm formation. Results: lower amount of biofilm formation was observed in the MC group than in the CB and SLB groups (p<0.0001). SLB and CB showed similar biofilm formation rates (p=0.855). In general, the cross-sectional wires .018"x.025" showed higher biofilm formation when associated with the three types of brackets. When brackets, wires, and ligatures were associated, the sets with NiCr wires and SSL ligatures with MC brackets (p=0.0008) and CB (p=0.0003) showed higher biofilm formation. Conclusion: thus, brackets of MC with NiTi and NiCr wires showed lower biofilm formation, regardless of the ligature and cross-sectional or gauge of the wire and, MC and CB brackets with NiCr wires and SSL ligatures were more likely to accumulate biofilms (AU)


Objetivo: neste estudo, a formação de biofilme por Candida albicans em aparelhos ortodônticos fixos foi avaliada. Material e Métodos: um total de 300 bráquetes metálicos convencionais (MC), cerâmicos (CB), autoligados (SLB), com fios de níquel-titânio (NiTi) e níquel-cromo (NiCr) e tipos de ligaduras foram organizados em trinta grupos (n=10). Bráquetes, fios e ligaduras foram unidos, esterilizados, colocados em placas de 24 poços, contaminados com suspensões padronizadas de C. albicans (107 células/mL) e incubados a 37°C por 48 h para a formação de biofilmes. Os biofilmes formados foram rompidos por meio de um homogeneizador ultrassônico e suspensões foram diluídas e semeadas em ágar Sabouraud-dextrose para determinar as unidades formadoras de colônias por mL. A microscopia eletrônica de varredura foi realizada antes e após a formação do biofilme. Resultados: foi observada menor formação de biofilme no grupo MC em comparação aos grupos CB e SLB (p<0,0001). A formação de biofilme foi semelhante nos grupos SLB e CB (p=0,855). Em geral, os fios de seção transversal .018"x.025" apresentaram maior formação de biofilme quando associados aos três tipos de bráquetes. Os conjuntos com fios de NiCr e ligaduras SSL com bráquetes MC (p=0,0008) e CB (p=0,0003) apresentaram maior formação de biofilme. Conclusão: bráquetes MC com fios de NiTi e NiCr apresentaram menor formação de biofilme, independente da ligadura e secção transversal ou bitola do fio e, braquetes MC e CB com fios de NiCr e ligaduras SSL foram mais propensos a acumular biofilmes.(AU)


Subject(s)
Candida albicans , Microscopy, Electron, Scanning , Orthodontic Brackets , Biofilms , Orthodontic Appliances, Fixed
9.
São José dos Campos; s.n; 2023. 85 p. tab, ilus.
Thesis in Portuguese | LILACS, BBO | ID: biblio-1416795

ABSTRACT

Extratos de plantas têm demonstrado diversos efeitos positivos para a saúde, incluindo ação antimicrobiana, no entanto, o uso clínico da fitoterapia ainda é discreto, de modo que mais estudos sobre os efeitos benéficos do sinergismo farmacológico de extratos poderiam contribuir para sua aplicação terapêutica. O objetivo deste estudo foi avaliar os efeitos dos extratos glicólicos de gengibre (EG) e quilaia (EQ) isolados e em associação sobre 7 cepas clínicas de Pseudomonas aeruginosa e uma cepa padrão em forma planctônica e biofilmes monotípicos. Para a análise antimicrobiana sobre cultura planctônica foram feitos testes para determinação de Concentração Inibitória Mínima (CIM) e Concentração Microbicida Mínima (CMM) (CLSI, M07-A9) dos extratos isolados, além do Índice de Concentração Inibitória Fracionada (ICIF) e do Índice de Concentração Microbicida Fracionada (ICMF) para os extratos combinados. A análise estatística foi feita com método ANOVA e teste de Tukey para dados com distribuição normal e Kruskall-Wallis com Teste de Comparação Múltipla de Dunn para dados sem distribuição normal (significância de 5%). Para cepa padrão foram determinadas CIM igual a 3,12 mg/mL e CMM igual a 6,25 mg/mL para ambos os extratos. Para cepas clínicas as CIM do EG foram 3,12 ou 6,25 mg/mL e de EQ 1,56 ou 3,12 mg/mL, enquanto os valores de CMM foram de 6,25 mg/mL para EG e de 1,56, 3,12 ou 6,25 mg/mL para EQ. Os resultados de ICIF indicaram 15 associações sinérgicas e 4 associações aditivas dos extratos contra a cepa padrão e, dentre cepas clínicas, foram obtidos 15 resultados aditivos. A partir dos resultados de ICMF foram identificadas 6 associações sinérgicas e 1 associação aditiva contra a cepa padrão, além de 8 associações com efeito aditivo contra cepas clínicas. A partir dos resultados de testes em culturas planctônicas foi avaliada a ação antibiofilme sobre as cepas em que foram observadas reduções de viabilidade de 36,7 e 34% para o EG (50 e 25 mg/mL) e 51,3 e 51,4% para EQ (25 e 12,5 mg/mL) contra cepa padrão. As reduções em cepas clínicas variaram de 43 a 73% com EG e de 36 a 79% para EQ. As associações dos extratos promoveram reduções de viabilidade de 8 a 35% contra 5 das 7 cepas clínicas. Conclui-se que os extratos glicólicos de gengibre e quilaia apresentam ação antimicrobiana de forma isolada e combinados com efeito aditivo sobre a forma planctônica de cepas clínicas resistentes de P. aeruginosa. De forma isolada, os extratos apresentaram importante ação preventiva na formação dos biofilmes dessas cepas, podendo ser considerados potenciais fitoterápicos com aplicações terapêuticas para o combate das infecções por P. aeruginosa. (AU)


Plant extracts have demonstrated several positive health effects, including antimicrobial action, however, the clinical use of phytotherapy is still discreet, so that more studies on the beneficial effects of pharmacological synergism of extracts could contribute to its therapeutic application. The aim of this study was to evaluate the effects of glycolic extracts of ginger (EG) and quilaia (EQ) alone and in combination on 7 clinical strains of Pseudomonas aeruginosa and a standard strain in planktonic form and monotypic biofilms. For the antimicrobial analysis on planktonic culture, tests were performed to determine the Minimum Inhibitory Concentration (MIC) and Minimum Microbicidal Concentration (MMC) (CLSI, M07-A9) of the isolated extracts, in addition to the Fractional Inhibitory Concentration Index (FICI) and the Fractionated Microbicidal Concentration Index (FICM) for the combined extracts. Statistical analysis was performed using the ANOVA method and Tukey's test for data with normal distribution and Kruskall-Wallis with Dunn's Multiple Comparison Test for data without normal distribution (5% significance). For the standard strain, MIC were determined equal to 3.12 mg/mL and MMC equal to 6.25 mg/mL for both extracts. For clinical strains the MIC of EG were 3.12 or 6.25 mg/mL and 1.56 or 3.12 mg/mL of EQ, while the MMC values were 6.25 mg/mL for EG and 1.56, 3.12 or 6.25 mg/ml for EQ. The FICI results indicated 15 synergistic and 4 additive associations of the extracts against the standard strain and, among clinical strains, 15 additive results were obtained. From the FICM results, 6 synergistic and 1 additive association against the standard strain were identified, in addition to 8 associations with additive effect against clinical strains. Based on the results of tests on planktonic cultures, the antibiofilm action were evaluated on the strains in which viability reductions of 36 and 34% were observed for EG (50 and 25 mg/mL) and 51% were observed for EQ (25 and 12, 5 mg/mL) against the standard strain. Reductions in clinical strains ranged from 43 to 73% with EG and from 36 to 79% for EQ. Associations of extracts promoted viability reductions of 8 to 35% against 5 out of 7 clinical strains. It is concluded that the glycolic extracts of ginger and quilaia have antimicrobial action in isolation and combined with additive effect on the planktonic form of resistant clinical strains of P. aeruginosa. Isolated, the extracts showed an important preventive action in the formation of biofilms of these strains and may be considered potential herbal medicines with therapeutic applications to combat P. aeruginosa infections. (AU)


Subject(s)
Pseudomonas aeruginosa , Drug Resistance, Microbial , Biofilms , Phytotherapy , Anti-Bacterial Agents
10.
Braz. dent. sci ; 26(1): 1-9, 2023. ilus
Article in English | LILACS, BBO | ID: biblio-1416950

ABSTRACT

Objective: to investigate the antimicrobial effects of toothpastes containing bioactive surface pre-reacted glass particles (S-PRG) on S. mutans biofilms adherence, initial colonization and maturation. Material and Methods: a reference UA 159 and a clinical S. mutans (SM6) strain were used. Bovine enamel specimens were randomly allocated into the groups (n=5): toothpastes containing 0%; 1%; 5%; 20%; 30% S-PRG; positive control dentifrice (NaF+triclosan); and negative control (distilled water). For biofilm development, samples were placed in a 24-well plate containing artificial saliva (4h), followed by adding 1mL of artificial saliva, BHI broth and 225µL of S. mutans suspension. Treatments with toothpastes were applied previously or after 4h and 24h of biofilm formation. Samples were incubated for 48h at 37°C in 5%CO2 and biofilm was detached and seeded in Petri dishes for determining the number of viable cells. Data were analyzed by ANOVA and Tukey test (5%). Results: significantly lower microorganisms' adherence (p<0.05) was obtained for all S-PRG toothpastes, with similar results to NaF+triclosan for SM6 and 20 and 30%S-PRG groups exhibiting higher inhibition effect than the NaF+Triclosan for UA159. Antibacterial effect on the early-stage biofilm was also observed for the S-PRG groups, but was not superior to the NaF+Triclosan toothpaste. For the mature biofilm, the effective antimicrobial potential of S-PRG toothpastes was observed only for the SM6 clinical strain, but was not higher than the positive control. Conclusion: experimental S-PRG toothpastes were effective to inhibit S. mutans biofilm growth by exhibiting antimicrobial activity, being promising agents to prevent cariogenic biofilm development (AU)


Objetivo: investigar o efeito de dentifrícios contendo S-PRG sobre a colonização inicial e maturação de biofilmes de S. mutans. Material e Métodos: uma cepa de referência (UA 159) e uma cepa clínica de S. mutans (SM6) foram utilizadas. Espécimes de esmalte bovino foram alocados nos grupos (n=5): dentifrícios contendo 0%; 1%; 5%; 20% e 30%S-PRG; controle positivo (NaF+triclosan); e controle negativo (água destilada). Os espécimes foram inseridos em uma placa de 24 poços contendo saliva artificial (4h), seguido por adição de 1mL de saliva artificial, BHI, 225µL de suspensão de S. mutans e foram tratados com suspensões de dentifrícios antes ou depois de 4 e 24h da formação do biofilme. Os espécimes foram incubados por 48h e o biofilme foi removido dos espécimes e semeado em placas de Petri para contagem de UFC/mL. Os dados foram analisados por ANOVA e teste de Tukey (5%). Resultados: houve diminuição na adesão de microrganismos para os grupos tratados com S-PRG (p<0.05). Para SM6, os dentifrícios contendo S-PRG apresentaram resultados semelhantes ao NaF+triclosan e para a cepa UA159 o dentifrício com 30%S-PRG apresentou efeito superior. Efeito antimicrobiano no biofilme recém-formado (4h) foi observado para os grupos contendo S-PRG, mas não foi observado efeito superior ao NaF+Triclosan. Para o biofilme maduro, efeito antimicrobiano do S-PRG foi observado apenas para a cepa clínica, mas não superior ao efeito do NaF+Triclosan. Conclusão: dentifrícios contendo S-PRG foram eficazes na inibição do desenvolvimento de biofilmes de S. mutans, sendo promissores agentes para prevenir o desenvolvimento de biofilme cariogênico. (AU)


Subject(s)
Animals , Cattle , Streptococcus mutans , Biofilms , Dental Enamel , Dental Plaque , Dentifrices
11.
Acta Medica Philippina ; : 53-60, 2023.
Article in English | WPRIM | ID: wpr-1003634

ABSTRACT

Background and Objective@#Staphylococcus aureus is the leading cause of skin and soft tissue infections such as abscesses, furuncles, and cellulitis. Biofilm forming strains of S. aureus have higher incidence of antimicrobial resistance to at least three or more antibiotics and are considered as multidrug resistant. Since S. aureus biofilm-producing strains have higher rates of multidrug and methicillin resistance compared to non-biofilm-producing strains, the need for alternative therapeutic option is important. Furthermore, rates of methicillin-resistant Staphylococcus aureus (MRSA) in Asia remain high. Results of the study may provide support for the clinical uses of P. betle as a topical antibacterial and antiseptic in the treatment and prevention of infections involving the skin, mouth, throat, and indwelling medical devices. Thus, this study aimed to evaluate the in vitro antibacterial and antibiofilm activities of Piper betle L. ethanolic leaf extract (PBE) against a biofilm-forming methicillin-sensitive Staphylococcus aureus ATCC 29213 (MSSA).@*Methods@#The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of PBE against MSSA were determined using the agar dilution assay. The biofilm inhibition and eradication assays using crystal violet were done to quantify the antibiofilm activities of PBE on MSSA biofilm.@*Results@#PBE showed activity against MSSA in agar dilution assay with MIC and MBC values of 2500 μg/mL and 5000 μg/mL, respectively. At subinhibitory concentrations, PBE showed biofilm inhibition activity at 1250 μg/mL but a lower percent eradication of biofilms as compared to oxacillin was noted.@*Conclusion@#PBE showed antibacterial activities including biofilm inhibition against methicillin-sensitive Staphylococcus aureus ATCC 29213 (MSSA).


Subject(s)
Piper betle , Staphylococcus aureus , Anti-Bacterial Agents , Biofilms
12.
Journal of Peking University(Health Sciences) ; (6): 38-43, 2023.
Article in Chinese | WPRIM | ID: wpr-971271

ABSTRACT

OBJECTIVE@#To construct a model of Enterococcus faecalis (E. faecalis) infection in dentinal tubules by gradient centrifugation and to evaluate the antibacterial effect of low-temperature plasma on E. faecalis in dentinal tubules.@*METHODS@#Standard dentin blocks of 4 mm×4 mm×2 mm size were prepared from single root canal isolated teeth without caries, placed in the E. faecalis bacterial solution, centrifuged in gradient and incubated for 24 h to establish the model of dentinal tubule infection with E. faecalis. The twenty dentin blocks of were divided into five groups, low-temperature plasma jet treatment for 0, 5 and 10 min, calcium hydroxide paste sealing for 7 d and 2% chlorhexidine gel sealing for 7 d. Scanning electron microscopy and confocal laser scanning microscope were used to assess the infection in the dentinal tubules and the antibacterial effect of low-temperature plasma.@*RESULTS@#The results of scanning electron microscopy and confocal laser scanning microscopy showed that after 24 h of incubation by gradient centrifugation, E. faecalis could fully enter the dentinal tubules to a depth of more than 600μm indicating that this method was time-saving and efficient and could successfully construct a model of E. faecalis infection in dentinal tubules. Low-temperature plasma could enter the dentinal tubules and play a role, the structure of E. faecalis was still intact after 5 min of low-temperature plasma treatment, with no obvious damage, and after 10 min of low-temperature plasma treatment, the surface morphology of E. faecalis was crumpled and deformed, the cell wall was seriously collapsed, and the normal physiological morphology was damaged indicating that the majority of E. faecalis was killed in the dentinal tubules. The antibacterial effect of low-temperature plasma treatment for 10 min exceeded that of the calcium hydroxide paste sealing for 7 d and the 2% chlorhexidine gel sealing for 7 d. These two chemicals had difficulty entering deep into the dentinal tubules, and therefore only had a few of antibacterial effect on the bacterial biofilm on the root canal wall, and there was also no significant damage to the E. faecalis bacterial structure.@*CONCLUSION@#Gradient centrifugation could establish the model of E. faecalis dentin infection successfully. Low-temperature plasma treatment for 10 min could kill E. faecalis in dentinal tubules effectively, which is superior to the calcium hydroxide paste sealing for 7 d and the 2% chlorhexidine gel sealing for 7 d.


Subject(s)
Chlorhexidine/pharmacology , Calcium Hydroxide/pharmacology , Enterococcus faecalis/physiology , Temperature , Dentin , Biofilms , Anti-Bacterial Agents/pharmacology , Root Canal Irrigants/pharmacology , Dental Pulp Cavity
13.
Braz. j. biol ; 83: 1-5, 2023. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1468851

ABSTRACT

Linum usitatissimum L is a widely used traditionally for multiple ailments. The present research was carried out to explore the antimicrobial, and anti-biofilm activity of crude extract of Linum usitatissimum L (Lu. Cr). Phytochemical and proximate analyses were performed. The bandages of diabetic foot patients were collected from the various hospitals. The bandages were cultured to isolate the bacterial strains present on it. The disc diffusion method was used to identify the antimicrobial potential whereas the minimum inhibitory concentration of the Lu.Cr were also determined. Proximate analysis confirms moisture content 8.33%, ash content 4.33%, crude protein 21.20%, crude fat 49.2% and crude fiber 5.63%. It was revealed that Gram-positive bacteria are most prevalent among all study groups. Lu.Cr possess significant bactericidal potential against S. aureus among all other microbes. Owing to this potential, linseed coated bandages can be used alternatively for the treatment of diabetic foot.


Linum usitatissimum L é amplamente utilizado tradicionalmente para doenças múltiplas. O presente trabalho foi realizado para explorar a atividade antimicrobiana e antibiofilme do extrato bruto de Linum usitatissimum L (Lu.Cr). Foram realizadas análises fitoquímicas e aproximadas. As ataduras de pacientes diabéticos com pé foram recolhidas nos vários hospitais. As bandagens foram cultivadas para isolar as cepas bacterianas presentes nas mesmas. O método de difusão em disco foi utilizado para identificar o potencial antimicrobiano e a concentração inibitória mínima do Lu.Cr também foi determinada. A análise aproximada confirma o teor de umidade 8,33%, teor de cinzas 4,33%, proteína bruta 21,20%, gordura bruta 49,2% e fibra bruta 5,63%. Foi revelado que as bactérias Gram-positivas são mais prevalentes entre todos os grupos de estudo. Lu.Cr possui potencial bactericida significativo contra S. aureus entre todos os outros micróbios. Devido a esse potencial, as ligaduras revestidas com linhaça podem ser utilizadas alternativamente para o tratamento do pé diabético.


Subject(s)
Male , Female , Humans , Adult , Biofilms/growth & development , Flax , Diabetic Foot
14.
Braz. j. biol ; 83: e245807, 2023. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1285613

ABSTRACT

Abstract Linum usitatissimum L is a widely used traditionally for multiple ailments. The present research was carried out to explore the antimicrobial, and anti-biofilm activity of crude extract of Linum usitatissimum L (Lu. Cr). Phytochemical and proximate analyses were performed. The bandages of diabetic foot patients were collected from the various hospitals. The bandages were cultured to isolate the bacterial strains present on it. The disc diffusion method was used to identify the antimicrobial potential whereas the minimum inhibitory concentration of the Lu.Cr were also determined. Proximate analysis confirms moisture content 8.33%, ash content 4.33%, crude protein 21.20%, crude fat 49.2% and crude fiber 5.63%. It was revealed that Gram-positive bacteria are most prevalent among all study groups. Lu.Cr possess significant bactericidal potential against S. aureus among all other microbes. Owing to this potential, linseed coated bandages can be used alternatively for the treatment of diabetic foot.


Resumo Linum usitatissimum L é amplamente utilizado tradicionalmente para doenças múltiplas. O presente trabalho foi realizado para explorar a atividade antimicrobiana e antibiofilme do extrato bruto de Linum usitatissimum L (Lu.Cr). Foram realizadas análises fitoquímicas e aproximadas. As ataduras de pacientes diabéticos com pé foram recolhidas nos vários hospitais. As bandagens foram cultivadas para isolar as cepas bacterianas presentes nas mesmas. O método de difusão em disco foi utilizado para identificar o potencial antimicrobiano e a concentração inibitória mínima do Lu.Cr também foi determinada. A análise aproximada confirma o teor de umidade 8,33%, teor de cinzas 4,33%, proteína bruta 21,20%, gordura bruta 49,2% e fibra bruta 5,63%. Foi revelado que as bactérias Gram-positivas são mais prevalentes entre todos os grupos de estudo. Lu.Cr possui potencial bactericida significativo contra S. aureus entre todos os outros micróbios. Devido a esse potencial, as ligaduras revestidas com linhaça podem ser utilizadas alternativamente para o tratamento do pé diabético.


Subject(s)
Humans , Diabetic Foot/drug therapy , Flax , Diabetes Mellitus , Staphylococcus aureus , Plant Extracts/pharmacology , Microbial Sensitivity Tests , Biofilms , Methanol
15.
Journal of Southern Medical University ; (12): 257-264, 2023.
Article in Chinese | WPRIM | ID: wpr-971523

ABSTRACT

OBJECTIVE@#To investigate the inhibitory effects of levofloxacin (LEV) combined with cellulase against bacille CalmetteGuerin (BCG) biofilms in vitro.@*METHODS@#The mature growth cycle of BCG biofilms was determined using the XTT method and crystal violet staining. BCG planktonic bacteria and BCG biofilms were treated with different concentrations of LEV and cellulose alone or jointly, and the changes in biofilm biomass were quantified with crystal violet staining. The mature BCG biofilm was then treated with cellulase alone for 24 h, and after staining with SYTO 9 and Calcofluor White Stain, the number of viable bacteria and the change in cellulose content in the biofilm were observed with confocal laser scanning microscopy. The structural changes of the treated biofilm were observed under scanning electron microscopy.@*RESULTS@#The MIC, MBC and MBEC values of LEV determined by broth microdilution method were 4 μg/mL, 8 μg/mL and 1024 μg/mL, respectively. The combined treatment with 1/4×MIC LEV and 2.56, 5.12 or 10.24 U/mL cellulase resulted in a significant reduction in biofilm biomass (P < 0.001). Cellulase treatments at the concentrations of 10.24, 5.12 and 2.56 U/mL all produced significant dispersion effects on mature BCG biofilms (P < 0.001).@*CONCLUSION@#LEV combined with cellulose can effectively eradicate BCG biofilm infections, suggesting the potential of glycoside hydrolase therapy for improving the efficacy of antibiotics against biofilmassociated infections caused by Mycobacterium tuberculosis.


Subject(s)
Levofloxacin/pharmacology , Gentian Violet/pharmacology , BCG Vaccine/pharmacology , Anti-Bacterial Agents/pharmacology , Biofilms , Cellulases/pharmacology , Microbial Sensitivity Tests
16.
Chinese Journal of Stomatology ; (12): 603-608, 2023.
Article in Chinese | WPRIM | ID: wpr-986122

ABSTRACT

Oligonucleotide drugs have the characteristics of targeting, modifiability and high biosafety. Recent studies have shown that oligonucleotide can be used to make biosensors, vaccine adjuvants, and has the functions of inhibiting alveolar bone resorption, promoting jaw and alveolar bone regeneration, anti-tumor, destroying plaque biofilm, and precise control of drug release. Therefore, it has a broad application prospect in the field of stomatology. This article reviews the classification, action mechanism and research status of oligonucleotide in stomatology. The aim is to provide ideas for further research and application of oligonucleotide.


Subject(s)
Humans , Alveolar Bone Loss , Biofilms , Bone Regeneration , Oligonucleotides , Oral Medicine
17.
Chinese Journal of Biotechnology ; (12): 881-897, 2023.
Article in Chinese | WPRIM | ID: wpr-970411

ABSTRACT

Facing the increasingly severe energy shortage and environmental pollution, electrocatalytic processes using electroactive microorganisms provide a new alternative for achieving environmental-friendly production. Because of its unique respiratory mode and electron transfer ability, Shewanella oneidensis MR-1 has been widely used in the fields of microbial fuel cell, bioelectrosynthesis of value-added chemicals, metal waste treatment and environmental remediation system. The electrochemically active biofilm of S. oneidensis MR-1 is an excellent carrier for transferring the electrons of the electroactive microorganisms. The formation of electrochemically active biofilm is a dynamic and complex process, which is affected by many factors, such as electrode materials, culture conditions, strains and their metabolism. The electrochemically active biofilm plays a very important role in enhancing bacterial environmental stress resistance, improving nutrient uptake and electron transfer efficiency. This paper reviewed the formation process, influencing factors and applications of S. oneidensis MR-1 biofilm in bio-energy, bioremediation and biosensing, with the aim to facilitate and expand its further application.


Subject(s)
Bioelectric Energy Sources/microbiology , Biofilms , Electrodes , Electron Transport , Shewanella/metabolism
18.
Rev. odontol. UNESP (Online) ; 52: e20230033, 2023. tab, il
Article in English | LILACS, BBO | ID: biblio-1530308

ABSTRACT

Introdução: O emprego de biofilmes polimicrobianos, utilizando a saliva como inóculo, é um modelo promissor para o estudo de biofilmes cariogênicos in vitro. Entretanto, ainda não existe uma padronização para seleção de doadores de saliva. Objetivo: O objetivo deste estudo foi estabelecer uma metodologia para seleção de doadores de saliva utilizando fatores salivares microbianos e características in vitro do biofilme. Material e método: Para doação de saliva foram selecionados vinte voluntários. Os voluntários permaneceram 24 horas sem escovar os dentes e ficaram em jejum por 2 horas antes da coleta da saliva. Foram avaliados os seguintes parâmetros: viabilidade das bactérias anaeróbias totais e mutans streptococci; concentração inibitória mínima (CIM) e concentração bactericida mínima (CBM) da clorexidina; capacidade de formação de biofilme por meio da biomassa; e a suscetibilidade dos biofilmes à clorexidina. Resultado: A viabilidade bacteriana da saliva, a capacidade de formação de biofilme e a suscetibilidade do biofilme à clorexidina foram apresentadas como média e intervalo de confiança (95%). A diferença entre a viabilidade do biofilme (mutans streptococci e bactérias totais) após tratamento com NaCl 0,9% e diacetato de clorexidina 0,2% foi comparada pelo teste t de Student com nível de significância estabelecido em 5%. A viabilidade total de bactérias anaeróbias (mediana) foi de 7,28 log 1+UFC/mL (unidades formadoras de colônia/mL). A viabilidade dos mutans streptococci na saliva apresentou mediana de 5,47 log 1+UFC/mL. Para capacidade de formação de biofilme a mediana da biomassa foi de 0,1172 A570. Conclusão: O tratamento com clorexidina reduziu significativamente os mutans streptococci e a viabilidade total das bactérias. A metodologia para seleção do doador de saliva foi estabelecida com sucesso.


Introduction: The utilization of polymicrobial biofilms, with saliva as an inoculum, represents a promising model for in vitro studies on cariogenic biofilms. However, there is still no standardization for selecting saliva donors. Objective: The aim of this study is to establish a methodology for the selection of saliva donors using microbial salivary factors and in vitro biofilm characteristics. Material and method: For saliva donation, twenty volunteers were selected. Volunteers remained 24 h without brushing their teeth and fasted for 2 h before saliva collection. The following parameters were evaluated: total anaerobic bacteria and mutans streptococci viability; minimum inhibitory concentration (MIC) and minimum bactericide concentration (MBC) of chlorhexidine; biofilm forming capacity by biomass assessment; and the susceptibility of biofilms to chlorhexidine. Result: Saliva bacterial viability, biofilm forming capacity and biofilm susceptibility to chlorhexidine were presented as mean and confidence interval (95%). The difference between biofilm (mutans streptococci and Total bacteria) viability after treatment with NaCl 0.9% and 0.2% chlorhexidine diacetate was compared using the Student t-test with a significance level established at 5%. Total anaerobic bacteria viability (median) was 7.28 log 1+CFU/mL (colony forming units/ mL). Mutans streptococci viability in the saliva showed a median of 5.47 log 1+CFU/mL. Biofilm forming capacity showed that biomass had a median of 0.1172 A570. Conclusion: Treatment with chlorhexidine significantly reduced mutans streptococci and total bacteria viability. The methodology for the selection of the saliva donor was successfully established.


Subject(s)
Humans , Male , Female , Saliva , Streptococcus mutans , Chlorhexidine , Biomass , Biofilms , Microbial Viability , Data Interpretation, Statistical
19.
Hig. Aliment. (Online) ; 37(297): e1135, jul.-dez. 2023.
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1531971

ABSTRACT

Os biofilmes podem acarretar vários problemas em diversas áreas, principalmente na indústria alimentícia, já que sua colonização nas superfícies como aço inoxidável, polietileno, polipropileno, podem contaminar produtos, causando sua deterioração, além de possibilitar diversas doenças transmitidas por alimentos. A formação de biofilme pode ocorrer em diferentes tipos de superfícies e pode envolver diversos micro-organismos. Práticas adequadas de higienização na indústria de alimentos são fundamentais para manter medidas seguras de preservação da qualidade dos alimentos. Portanto, o presente estudo objetiva realizar levantamento bibliográfico para explanar a formação e caracterização de biofilmes, ressaltando sua importância na indústria de alimentos e os métodos de prevenção de biofilmes disponíveis. Pode-se observar a necessidade de novas tecnologias no controle e erradicação de Biofilmes.


Biofilms can cause several problems in different areas, especially in food, as their colonization on surfaces in industries such as stainless steel, polyethylene, polypropylene, can contaminate products, causing their deterioration, in addition to making various food-borne diseases possible. Biofilm formation can occur on different types of surfaces and can involve different microorganisms. Adequate hygiene practices in the food industry are essential to maintain safe measures to preserve food quality. Therefore, the present study aims to carry out a bibliographical survey to explain the formation and characterization of biofilms, highlighting the importance in the food industry and the available biofilm prevention methods. The need for new technologies in the control and eradication of Biofilms can be observed.


Subject(s)
Food Quality , Biofilms , Food Industry , Food Microbiology , Noxae
20.
Braz. j. oral sci ; 22: e231137, Jan.-Dec. 2023. ilus
Article in English | LILACS, BBO | ID: biblio-1523140

ABSTRACT

The purpose of this in vitro study was to analyze the influence of nicotine on the extracellular polysaccharides in Fusobacterium nucleatum biofilm. Methods: F. nucleatum (ATCC 10953) biofilms supplemented with different concentrations of nicotine (0, 0.5, 1, 2, 4, and 8 mg/mL) were grown in two different BHI broth conditions [no sucrose and 1% sucrose]. Extracellular polysaccharides assay, pH measurements, and a spectrophotometric assay were performed. Data were submitted for ANOVA and Tukey honestly significant difference analyses (HSD) tests (α =.05). Results: Extracellular polysaccharides synthesis was influenced by an interaction between nicotine concentrations and growth medium solution containing sucrose (P<.05). The pH values declined in the sucrose-exposed biofilm were greater than in the group exposed only to nicotine (P<.05). The biofilm exposed to sucrose and nicotine had a higher total biofilm growth (P<.05) than the nicotine-treated biofilm without sucrose. Conclusions: Regardless of sucrose exposure, biofilms exposed to different nicotine concentrations influenced the amount of extracellular polysaccharides


Subject(s)
Humans , Polysaccharides, Bacterial/chemical synthesis , Fusobacterium nucleatum/growth & development , Biofilms/growth & development , Nicotine/pharmacology , Periodontal Diseases/microbiology , Spectrophotometry , Sucrose/administration & dosage , Culture Media , Dental Caries/microbiology , Nicotine/administration & dosage
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