Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 104
Filter
1.
Arq. ciências saúde UNIPAR ; 27(2): 740-794, Maio-Ago. 2023.
Article in Portuguese | LILACS | ID: biblio-1424949

ABSTRACT

A imaturidade do sistema imunológico, associado a Determinantes Sociais de Saúde (DSS), promove doenças na infância. Especificamente, na cavidade oral, os DSS, representados pelo consumo elevado de açúcar, limitado acesso aos serviços de saúde e deficiência na higiene bucal, favorecem transtornos locais e sistêmicos. Assim, o estudo objetivou associar os DSS, no contexto das condições socioeconômicas, do acompanhamento pelo serviço de saúde e dos aspectos relacionados à saúde bucal de crianças atendidas em Unidades Básicas de Saúde (UBS) de um município cearense. Trata-se de estudo observacional, analítico, transversal e de abordagem quantitativa, conduzido com crianças e suas mães em Acarape - CE. Após consentimento, essas preencheram um questionário. Os dados foram analisados. Das 70 mães, 87,14% e 90,00% tinham idade inferior ou igual a 30 anos e renda de até um salário mínimo, respectivamente. Das 70 crianças, 87,14% tinham seus dentes/gengiva higienizados por seus pais ou responsável. Do total, 94,29% nunca se submeteram a atendimento odontológico. Observou-se associação significativa entre a mãe ter escolaridade superior ao ensino fundamental incompleto e higienizar os dentes/gengiva do filho com escova dental e dentifrício. Constatou-se associação significativa entre a criança ingerir bolacha doce/recheada, não consumir refrigerante e usar escova dental e dentifrício na higienização oral. Conclui-se que as crianças eram acompanhadas nas UBS regularmente; no entanto, esse serviço não esteve relacionado ao atendimento odontológico. Apesar da ausência desse tipo de acompanhamento e do consumo de alimentos cariogênicos, as mães se preocupavam com a saúde bucal das crianças, higienizando a cavidade oral diariamente, com meios adequados.


The immaturity of the immune system, associated with Social Determi- nants of Health (SDH), promotes diseases in childhood. Specifically, in the oral cavity, SDH, represented by high sugar consumption, limited access to health services, and poor oral hygiene, favors local and systemic disorders. Thus, the study aimed to associate the SDH, in the context of socioeconomic conditions, monitoring by the health service and aspects related to children's oral health assisted in Basic Health Units (BHU) of a muni- cipality in Ceará. This is an observational, analytical, cross-sectional study with a quan- titative approach conducted with children and their mothers in Acarape - CE. After con- sent, they filled out a questionnaire. Data were analyzed. Of the 70 mothers, 87.14% and 90.00% were aged less than or equal to 30 years and had income up to one minimum wage, respectively. Of the 70 children, 87.14% had their teeth/gums cleaned by their pa- rents or guardian. Of the total, 94.29% never underwent dental care. There was a signifi- cant association between the mother having higher education than incomplete elementary school and cleaning the child's teeth/gums with a toothbrush and toothpaste. A significant association was found between the child eating sweet/stuffed biscuits, not consuming soft drink, and using a toothbrush and dentifrice for oral hygiene. It is concluded that the chil- dren were regularly monitored at the BHU; however, this service was not related to dental care. Despite the absence of this type of follow-up and the consumption of cariogenic foods, the mothers were concerned about their children's oral health, cleaning the oral cavity daily with adequate means.


La inmadurez del sistema inmunológico, asociada a los Determinantes So- ciales de la Salud (DSS), promueve enfermedades en la infancia. Específicamente, en la cavidad bucal, los DSS, representados por el alto consumo de azúcar, el acceso limitado a los servicios de salud y la mala higiene bucal, favorecen los trastornos locales y sisté- micos. Así, el estudio tuvo como objetivo asociar el DSS, en el contexto de las condicio- nes socioeconómicas, el seguimiento por el servicio de salud y los aspectos relacionados con la salud bucal de los niños atendidos en las Unidades Básicas de Salud (UBS) de un municipio de Ceará. Se trata de un estudio observacional, analítico, transversal con enfo- que cuantitativo, realizado con niños y sus madres en Acarape ­ CE. Después del consen- timiento, completaron un cuestionario. Los datos fueron analizados. De las 70 madres, 87,14% y 90,00% tenían edad menor o igual a 30 años e ingresos hasta un salario mínimo, respectivamente. De los 70 niños, al 87,14% se les limpió los dientes/encías por sus pa- dres o tutores. Del total, el 94,29% nunca realizó atención odontológica. Hubo una aso- ciación significativa entre la madre con educación superior a la primaria incompleta y la limpieza de los dientes/encías del niño con cepillo y pasta dental. Se encontró una asoci- ación significativa entre el niño que come galletas dulces/rellenas, no consume gaseosas y usa cepillo de dientes y dentífrico para la higiene bucal. Se concluyó que los niños eran monitoreados periódicamente en la UBS; sin embargo, este servicio no estaba relacionado con el cuidado dental. A pesar de la ausencia de este tipo de seguimiento y del consumo de alimentos cariogénicos, las madres se preocupan por la salud bucal de sus hijos, reali- zando la limpieza de la cavidad bucal diariamente, con medios adecuados.


Subject(s)
Humans , Male , Female , Adult , Oral Hygiene/instrumentation , Cariogenic Agents/analysis , Child Care/supply & distribution , Unified Health System , Candy , Cross-Sectional Studies/methods , Dental Care/instrumentation , Health Services Accessibility , Mothers
2.
Arq. ciências saúde UNIPAR ; 27(1): 332-358, Jan-Abr. 2023.
Article in Portuguese | LILACS | ID: biblio-1414877

ABSTRACT

Fatores de risco, incluindo os de cunho materno, interferem no crescimento e desenvolvimento infantil. Nesse sentido, o conhecimento desses fatores e avaliação ade- quada desses processos são necessários para articulação de estratégias preventivas de transtornos futuros. Assim, o estudo visou investigar o crescimento e desenvolvimento de crianças atendidas em consulta de puericultura em unidades básicas de saúde de um mu- nicípio cearense, que integra uma universidade brasileira de cunho internacional, e seus fatores de risco. Trata-se de estudo observacional, analítico, transversal e de abordagem quantitativa, conduzido com crianças e suas mães no Centro de Saúde de Acarape e Posto de Saúde São Benedito (Acarape ­ CE), no período de fevereiro a julho de 2021. Após consentimento, as mães preencheram um questionário, seguido de avaliação do cresci- mento e desenvolvimento das crianças. Os dados obtidos foram analisados. Das 70 crian- ças, 50,00% (n = 17) e 51,43% (n = 18) dos meninos e meninas tinham baixa estatura para idade, respectivamente. Para o desenvolvimento psicossocial, dos 284 testes condu- zidos, 86,27% (n = 245) foram realizados em plenitude pelas crianças. Observou-se as- sociação significativa entre a gestante ter concebido o filho com, no mínimo, 9 meses de período gestacional e esse apresentar relação Peso/Idade adequada e Estatura/Idade ina- dequada. Houve associação significativa entre a criança não ingerir refresco em pó e apre- sentar relação Peso/Idade adequada. Conclui-se que as crianças tinham idade gestacional adequada e apresentavam estado nutricional apropriado, apesar da baixa estatura para idade. Manifestavam desenvolvimento psicossocial, de linguagem e físico normais. Sobre os fatores de risco, esses envolveram os de cunho materno e o consumo de alimentos cariogênicos.


Risk factors, including maternal ones, interfere with child growth and de- velopment. In this sense, knowledge of these factors and adequate evaluation of these processes are necessary to articulate preventive strategies for future disorders. Thus, the study aimed to investigate the growth and development of children seen in childcare con- sultations in primary health units in Ceará, part of a Brazilian university of international character, and their risk factors. This is an observational, analytical, cross-sectional study with a quantitative approach, conducted with children and their mothers at the Acarape Health Center and São Benedito Health Center (Acarape ­ CE), from February to July 2021. After consent, the mothers completed a questionnaire, followed by an assessment of the children's growth and development. The data obtained were analyzed. Of the 70 children, 50.00% (n = 17) and 51.43% (n = 18) of the boys and girls were short for their age, respectively. Of the 284 tests conducted for psychosocial development, 86.27% (n = 245) were entirely performed by the children. A significant association was observed between the pregnant woman having conceived her child at least nine months of gestation and having an adequate Weight/Age and Height/Age ratio. There was a significant asso- ciation between the child not ingesting powdered soft drinks and having an appropriate Weight/Age ratio. It was concluded that the children had an adequate gestational age and had an appropriate nutritional status, despite their low height for their age. They showed normal psychosocial, language, and physical development. The risk factors involved those of mother nature and the consumption of cariogenic foods.


Los factores de riesgo, incluidos los maternos, interfieren en el crecimiento y desarrollo infantil. En este sentido, el conocimiento de estos factores y la evaluación adecuada de estos procesos son necesarios para articular estrategias preventivas de futu- ros trastornos. Así, el estudio tuvo como objetivo investigar el crecimiento y desarrollo de niños atendidos en consultas de puericultura en unidades básicas de salud de un muni- cipio de Ceará, que forma parte de una universidad brasileña de carácter internacional, y sus factores de riesgo. Se trata de un estudio observacional, analítico, transversal, con enfoque cuantitativo, realizado con niños y sus madres en el Centro de Salud de Acarape y el Centro de Salud São Benedito (Acarape ­ CE), de febrero a julio de 2021. Después del consentimiento, las madres completaron un cuestionario, seguido de una evaluación del crecimiento y desarrollo de los niños. Los datos obtenidos fueron analizados. De los 70 niños, el 50,00% (n = 17) y el 51,43% (n = 18) de los niños y niñas eran bajos para su edad, respectivamente. Para el desarrollo psicosocial, de las 284 pruebas realizadas, el 86,27% (n = 245) fueron realizadas íntegramente por los niños. Se observó una asociación significativa entre la gestante haber concebido a su hijo con al menos 9 meses de gesta- ción y tener una adecuada relación Peso/Edad y Talla/Edad. Hubo asociación significativa entre el niño que no ingiere gaseosas en polvo y presentar una adecuada relación Peso/Edad. Se concluyó que los niños tenían una edad gestacional adecuada y un estado nutricional adecuado, a pesar de su baja talla para su edad. Presentaron un desarrollo psi- cosocial, lingüístico y físico normal. En cuanto a los factores de riesgo, estos involucraron los de naturaleza materna y el consumo de alimentos cariogénicos.


Subject(s)
Humans , Male , Female , Child , Universities , Child Development/physiology , Risk Factors , Cariogenic Agents , Child , Nutritional Status/physiology , Cross-Sectional Studies/methods , Gestational Age , Maternal-Fetal Relations/physiology , Growth and Development/physiology , Eating/physiology
3.
Bol. malariol. salud ambient ; 62(3): 489-497, 2022. tab, graf
Article in Spanish | LILACS, LIVECS | ID: biblio-1397149

ABSTRACT

La caries dental y la placa dental se encuentran entre las enfermedades más comunes en todo el mundo y son causadas por una mezcla de microorganismos y restos de alimentos. Tipos específicos de bacterias productoras de ácido, especialmente Streptococcus mutans, colonizan la superficie dental y causan daño a la estructura dental dura en presencia de carbohidratos fermentables, por ejemplo, sacarosa y fructosa. Por otro lado, el sangrado posterior a la extracción es una complicación reconocida y frecuente en la práctica dental, que se define como la pérdida de sangre que continúa más allá de las 8 a 12 horas después de la exodoncia. Existe una amplia gama de técnicas sugeridas y sustancias para el tratamiento del sangrado post-extracción, que incluyen intervenciones dirigidas tanto a causas locales como sistémicas. El ácido tánico es una de las sustancias astringente que precipitan proteínas, pero no penetran en las células, por lo que inciden solo en la capa superficial. Su objetivo se enfoca a robustecer la superficie para acrecentar su resistencia mecánica y reducir la exudación. El objetivo de este estudio fue determinar la presencia de S. mutans en las biopelículas dentales y al mismo tiempo evaluar la actividad del ácido tánico como inhibidor del sangrado profuso en las extracciones dentales. S. mutans se aisló en el 92,5% de los pacientes evaluados. Los tiempos de hemostasia post-exodoncia fue significativamente menor en el grupo de pacientes a los que se les aplicó el ácido tánico en comparación a los que no se les aplicó(AU)


Tooth decay and dental plaque are among the most common diseases worldwide and are caused by a mix of microorganisms and food debris. Specific types of acid-producing bacteria, especially Streptococcus mutans, colonize the tooth surface and cause damage to hard tooth structure in the presence of fermentable carbohydrates, for example, sucrose and fructose. On the other hand, post-extraction bleeding is a recognized and frequent complication in dental practice, defined as blood loss that continues beyond 8 to 12 hours after extraction. There is a wide range of suggested techniques and substances for the treatment of post-extraction bleeding, including interventions targeting both local and systemic causes. Tannic acid is one of the astringent substances that precipitate proteins, but does not penetrate the cells, so it affects only the superficial layer. Its objective is focused on strengthening the surface to increase its mechanical resistance and reduce exudation. The objective of this study was to determine the presence of S. mutans in dental biofilms and at the same time to evaluate the activity of tannic acid as an inhibitor of profuse bleeding in dental extractions. S. mutans was isolated in 92.5% of the patients evaluated. Post-extraction hemostasis times were significantly shorter in the group of patients who received tannic acid compared to those who did not(AU)


Subject(s)
Humans , Male , Female , Streptococcus mutans , Surgery, Oral , Cariogenic Agents , Biofilms , Bacteria , Acids , Carbohydrates , Dental Plaque , Food , Fructose
4.
RFO UPF ; 26(1): 7-16, 20210327. tab, graf
Article in Portuguese | LILACS, BBO | ID: biblio-1428571

ABSTRACT

Objetivo: avaliar o potencial cariogênico de balas duras e mastigáveis e seu potencial desmineralizante em esmalte bovino. Métodos: foram selecionadas 30 balas de diferentes marcas, divididas em balas duras (n=11), Tic Tac®, Halls® e IceKiss®, e balas mastigáveis (n=19), Lílith®, Azedinha®, Mentos Rainbow® e Dori Gomets®. As balas foram dissolvidas em água destilada (1:10) e foram avaliados pH, acidez titulável (ATT) e presença de sólidos solúveis totais (SST/°Brix). Na ciclagem erosiva, 40 espécimes de esmalte bovino foram divididos em quatro grupos (n=10): GCN ­ saliva artificial; GCP ­ ácido clorídrico; GT1 ­ solução da bala Lílith® maçã verde; GT2 ­ solução da bala IceKiss® extraforte. O desafio erosivo foi realizado por 2 minutos, 4x/dia, segui-do de 2 horas de imersão em saliva artificial durante cinco dias. Resultados: os valores de pH para as balas duras e mastigáveis variaram de 2,88 a 5,53 e de 2,73 a 4,16, respectivamente. ATT em pH 5,5 variou de 0,07 mL a 39,40 mL de NaOH 0,1 N, para as balas duras, e de 1,53 mL a 35,83 mL, para balas mastigáveis. ATT em pH 7,0 variou de 0,2 mL a 49,13 mL de NaOH, para balas duras, e de 2,37 mL a 49,97 mL, para as mastigáveis. O conteúdo de SST de todas as balas duras foi superior a 8,5°Brix, já entre as mastigáveis variou de 5,3 a 8,83°Brix. O GCP apresentou maior desmineralização que GCN e GT2 (p<0,05). Conclusão: a maioria das balas duras e mastigáveis dissolvidas em água destilada mostraram-se potencialmente erosivas e cariogênicas.(AU)


Objective: evaluate the cariogenic potential of hard and soft candies and their demineralizing potential in bovine enamel. Methods: 30 candies of different brands were selected, divided into hard candies (n=11): Tic Tac®, Halls® and IceKiss® and soft candies (n=19): Lílith®, Azedinha®, Mentos Rainbow® and Dori Gomets®. The candies were dissolved in distilled water (1:10) and pH, titratable acidity (TT) and presence of total soluble solids (SST/°Brix) were evaluated. In erosive cycling, 40 specimens of bovine enamel were divided into four groups (n=10): GCN - artificial saliva; GCP - hydrochloric acid; GT1 - Lilith® apple green candy solution; GT2 - IceKiss® Extra Strong candy Solution. The erosive challenge was performed for 2 minutes, 4X/day, followed by 2 hours of immersion in artificial saliva for five days. Results: pH values for hard and soft candies ranged from 2.88 to 5.53 and 2.73 to 4, respectively. ATT at pH 5.5 varied from 0.07 mL to 39.40 mL of 0.1 N NaOH for hard candies and 1.53 mL to 35.83 mL for soft candies. ATT at pH 7,0 varied from 0.2 mL to 49.13 mL of 0.1 N NaOH for hard candies and from 2.37 mL to 49.97 mL for soft candies. The content of SST of all hard candies was higher than 8.5 °Brix and for soft candies, varied between 5.3 to 8.83 °Brix. The GCP group showed greater demineralization than GCN and GT2 (p<0.05). Conclusion: most hard and soft candies dissolved in distilled water were potentially erosive and cariogenic.(AU)


Subject(s)
Animals , Cattle , Candy , Cariogenic Agents/chemistry , Tooth Demineralization/etiology , Dental Enamel/chemistry , Saliva, Artificial/chemistry , Time Factors , Calcium Hydroxide/chemistry , Analysis of Variance , Statistics, Nonparametric , Acidity , Hydrogen-Ion Concentration
5.
RFO UPF ; 26(1): 78-83, 20210327. tab
Article in Portuguese | LILACS, BBO | ID: biblio-1428588

ABSTRACT

Objetivo: mensurar o potencial erosivo de gomas de mascar não convencionais disponíveis no mercado brasileiro. Material e método: estudo experimental (in vitro) de caráter quantitativo. Foram adquiridos oito sabores de gomas de mascar: Poosh® (pinta língua); Plutonita® (abraço congelante, dentada ardente, baba de bruxa e língua ácida); e TNT® (sangue, lava e tumba). Para análise da acidez, foram realizadas leituras em triplicata do pH e acidez titulável (AT), utilizando um potenciômetro e eletrodo combinado de vidro em soluções obtidas após a maceração das gomas de mascar em água duplamente deionizada. Nas soluções com valores de pH inferiores a 5,5, foi mensurada a AT adicionando-se alíquotas de 100 µLNaOH 0,1 M, até alcançar pH 5,5. Os resultados foram submetidos à Análise de Variância (ANOVA). As comparações das médias de pH e acidez titulável foram realizadas pelo teste de Tukey, com um nível de 5% de significância (p<0,05). Resultados: os valores de pH variaram entre 2,4 (abraço congelante) e 3,5 (baba de bruxa), diferin-do significantemente do controle (água mineral/pH=6,2). Os valores de acidez titulável variaram entre 628µL de NaOH 0,1 M (sangue) e 10700µL de NaOH 0,1 M (abraço congelante). A goma de mascar sabor abraço congelante apresentou o pH mais baixo e a mais elevada acidez titulável, diferindo significantemente dos demais sabores. Conclusões: as gomas de mascar avaliadas são ácidas, mas diferem quanto ao seu potencial erosivo. Seu consumo abusivo pode constituir um fator de risco para erosão dental.(AU)


Objective: to measure the erosive potential of non- -conventional chewing gums available in the Brazilian market. Material and methods: this is an in vitro study using the following flavors of chewing gums from the Brazilian market: Poosh® (tongue cleaner); Plutonita® (freezing hug, burning bite, witch slime and acid tongue); TNT® (blood, lava and tomb). For acidity analysis, pH and titratable acidity (TA) readings were performed in triplicate using a potentiometer and combined glass electrode in solutions obtained after maceration of the chewing gums in double deionized water. In solutions with pH values below 5.5, the TA was measured by adding aliquots of 100 µL 0.1 M NaOH, until pH 5.5 was reached. The results were submitted to Analysis of Variance (ANOVA). Comparisons of pH and titratable acidity means were performed by Tukey test at a 5% significance level (p<0.05). Results: the pH values that ranged from 2.4 (freezing hug) to 3.5 (witch's slime) differed significantly from the control (mineral water/ pH=6.2). Titratable acidity values ranged from 628µL of 0.1 M NaOH (blood) to 10700µL of 0.1 M NaOH (freezing hug). The freezing hug flavor chewing gum had the lowest pH and the highest titratable acidity differing significantly from the other flavors. Conclusions: the evaluated chewing gums are acidic, but differ in their erosive potential. Their abusive consumption may constitute a risk factor for dental erosion.(AU)


Subject(s)
Tooth Erosion/etiology , Cariogenic Agents/chemistry , Chewing Gum/adverse effects , Cariogenic Agents/adverse effects , Risk Factors , Analysis of Variance , Acidity , Hydrogen-Ion Concentration
6.
Article in Spanish | LILACS | ID: biblio-1177841

ABSTRACT

Introducción: La caries de la temprana infancia (CTI) es una de las enfermedades infecciosas más comunes en infantes y niños preescolares. Objetivo: Evaluar la caries de la temprana infancia y los factores de riesgo asociados con prácticas dietarías y el estado nutricional en niños de 10-48 meses de edad. Materiales y Métodos: Se realizó un estudio transversal en 142 niños de nivel socioeconómico medio-bajo de un centro infantil gubernamental en Cuenca, Ecuador. La salud dental, estado nutricional y patrones dietarios se evaluaron mediante exámenes clínicos, mediciones antropométricas y recordatorios de 24 horas, respectivamente. Resultados: La prevalencia de caries de la temprana infancia fue alta (95.8%) mayoritariamente en etapa avanzada (83.1%). No se observaron asociaciones con el estado nutricional. El consumo diario de alimentos cariogénicos fue significativamente mayor entre semana (11,1 ± 1,4) (p<0,001). La dieta fue categorizada como de bajo riesgo cariogénico (<34). La edad del niño (OR=1.1; IC 95%: 1.041, 1.160, p=0.001) y el índice de placa (OR=4.9; IC 95%: 1.331, 17.82; p=0.017) fueron determinantes positivos de caries avanzada. Conclusión: El deterioro de la salud dental de esta población se evidenció, empeorando a edades mayores sin asociación con los patrones dietarios.


Introduction. Early childhood caries (ECC) is one of the most common infectious diseases for infants and preschool children. Objective: To evaluate early childhood caries and risk factors associated with dietary practices and nutri- tional status in children of 10-48 months old. Materials and Methods: A cross-sectional study was performed on 142 children of medium-low so- cio-economic status from a governmental child-care center in Cuenca, Ecuador. Dental health, nutritional status and dietary patterns were assessed through clinical examination, anthropometric measurements and 24-hours dietary recalls, respectively. Results: Early childhood caries prevalence was high (95.8%) mostly of advanced stage (83.1%). No as- sociations with nutritional status. Daily consumption of cariogenic foods was significantly higher during weekdays (11.1 ± 1.4) (p<0.001). The diet was categorized as low cariogenic risk (<34). Child's age (OR=1.1; 95% CI: 1.041, 1.160, p=0.001) and the plaque index (OR=4.9; 95% CI: 1.331, 17.82; p=0.017) were positive determinants for advanced caries. Conclusions: The deteriorated dental health of this population was evidenced, being worse at older ages without any association with dietary patterns.


Introdução. A cárie da primeira infância (CPI) é uma das enfermidades infecciosas mais comuns em crianças pre-escolares. Objetivo: Avaliar a cárie da primeira infância e fatores de risco associados com práticas dietárias e estado nutricional de crianças de 10-48 meses. Materiais e métodos. Realizou-se um estudo transversal em 142 crianças de nível socioeconômico médio-baixo do centro infantil governamental em Cuenca, Equador. A saúde dental, estado nutricio- nal e patroes dietários se avaliaram mediante exames clínicos, medições antropométricas e lembretes de 24 horas respectivamente. Resultados: A prevalência de cárie da primeira infância foi alta (95.8%) principalmente em estágio avançado (83.1%). Não se observaram relações com o estado nutricional. O consumo diário de ali- mentos cariogênicos foi significativamente maior entre semana (11,1 ± 1,4) (p<0,001). A dieta foi categorizada como de baixo risco cariogênico (<34). A idade das crianças (OR=1.1; IC 95%: 1.041, 1.160, p=0.001) e o índice de placa (OR=4.9; IC 95%: 1.331, 17.82; p=0.017) foi determinante em cárie avançada positiva. Conclusão: O deterioro da saúde dental dessa população se evidenciou, piorando as idades mais velhas sem relação com os patroes dietários.


Subject(s)
Dental Caries , Cariogenic Agents , Child , Nutritional Status , Diet
7.
Rev. odontopediatr. latinoam ; 10(1): 73-81, 2020. tab
Article in Spanish | LILACS | ID: biblio-1147483

ABSTRACT

La adecuación del medio bucal consiste en un conjunto de medidas que apuntan a la disminución de los niveles de microorganismos cariogénicos presentes en la cavidad bucal, proporcionando la paralización del proceso carioso. Las medidas se aplican después del diagnóstico y antes del tratamiento restaurador definitivo, siendo considerada una etapa intermedia. El presente trabajo tiene como objetivo discutir la adecuación del medio bucal, sus etapas y presentar el protocolo utilizado en la Clínica de Odontopediatría de la UNIGRANRIO, RJ Brasil.


A adequação do meio bucal consiste em um conjunto de medidas que visam a diminuição dos níveis de microorganismos cariogênicos presentes na cavidade bucal, proporcionando a paralisação do processo da doença cárie. As medidas são aplicadas após o diagnóstico e anteriormente ao tratamento restaurador definitivo, sendo considerada uma etapa intermediária. O presente trabalho tem por objetivo discutir a adequação do meio bucal, suas etapas e apresentar o protocolo utilizado na Clínica de Odontopediatria da UNIGRANRIO, Brasil


The adequacy of the oral environment consists of a set of measures aimed at reducing the levels of cariogenic microorganisms present in the oral cavity and arresting the caries disease process. These measurements are applied after the diagnosis but before the definitive restorative treatment; hence, being considered an intermediate step. The aim of this study was to discuss the adequacy of the oral environment, its stages and present the related protocol used in the Pediatric Dentistry Clinic of UNIGRANRIO, Brazil


Subject(s)
Humans , Male , Infant , Child, Preschool , Child , Adolescent , Weights and Measures , Brazil , Cariogenic Agents , Preventive Dentistry , Pediatric Dentistry , Diagnosis
8.
Int. j. odontostomatol. (Print) ; 13(1): 93-96, mar. 2019. graf
Article in English | LILACS | ID: biblio-990071

ABSTRACT

ABSTRACT: The aim of the present study was to evaluate the effect of commercial sweeteners on root dentin demineralization using a microcosm biofilm model. Bovine dentin specimens with pre-determined surface hardness were randomized into six groups according to the studied sweeteners: sucralose, stevia, saccharin, aspartame. Sucrose was used as a positive control and an untreated group as a negative control. The specimens were submitted to biofilm development from one saliva donor and the cariogenic challenge occurred on subsequent five days, twice a day. At the end, the percentage of surface hardness loss (%SHL) and biomass was determined and submitted to ANOVA followed by Tukey's test. Sucrose presented the highest rate of demineralization, however, all sweeteners tested lead to a statistically higher root demineralization compared to the negative control (p <0.05). Sucrose caused greater demineralization in root dentin, however, the sweeteners were also able to induce it under this biofilm model.


RESUMEN: El objetivo del presente estudio fue evaluar el efecto de los edulcorantes comerciales en la desmineralización de la dentina radicular utilizando un modelo de biofilm microcosmo. Se asignaron al azar muestras de dentina bovina con una dureza de la superficie predeterminada de acuerdo con los edulcorantes estudiados: sucralosa, estevia, sacarina, aspartame. La sacarosa se utilizó como control positivo y un grupo no tratado como control negativo. Las muestras se enviaron al desarrollo de biopelículas de un donante de saliva y el desafío cariogénico se produjo en los siguientes cinco días, dos veces al día. Al final, se determinó el porcentaje de pérdida de dureza de la superficie (% PDS) y biomasa y se aplicó un estudio estadístico de ANOVA seguido de la prueba de Tukey. La sacarosa presentó la mayor tasa de desmineralización; sin embargo, todos los endulzantes probados condujeron a una desmineralización de la raíz estadísticamente mayor en comparación con el control negativo (p<0,05). La sacarosa causó una mayor desmineralización en la dentina de raíz, sin embargo, los edulcorantes también fueron capaces de inducirla bajo este modelo de biofilm.


Subject(s)
Animals , Cattle , Sweetening Agents/pharmacology , Tooth Root/drug effects , Cariogenic Agents/pharmacology , Tooth Demineralization/chemically induced , Dentin/drug effects , Tooth Root/microbiology , Analysis of Variance , Tooth Demineralization/microbiology , Biofilms/growth & development , Dietary Sucrose/pharmacology , Dentin/microbiology
9.
Braz. oral res. (Online) ; 33: e029, 2019. tab, graf
Article in English | LILACS | ID: biblio-1001599

ABSTRACT

Abstract: Orthodontic bonding systems are submitted to demineralization and remineralization dynamics that might compromise their surface smoothness, and favor biofilm aggregation and caries development. The aim of the present study was to evaluate the effects of a cariogenic challenge model (in vitro pH-cycling model) on the surface roughness and topography of 3 bonding materials: Transbond™ XT (XT), Transbond™ Plus Color Change (PLUS) and Fuji Ortho™ LC (FUJI), by means of Atomic Force Microscopy (AFM). Six specimens with standardized dimensions and surface smoothness were fabricated per group, and the materials were manipulated in accordance with the manufacturers' instructions. No polishing was necessary. AFM tests were performed before and after pH-cycling, taking 3 readouts per specimen. The roughness results (Ra) were obtained at nanometric levels (nm) and surface records were acquired in two- and three-dimensional images of height and lock-in phase of the material components. The surfaces of all groups analyzed in the study were morphologically altered, presenting images suggestive of matrix degradation and loss of matrix-load integrity. FUJI presented the greatest increase in surface roughness, followed by XT and PLUS, respectively (p≤0.001). Nevertheless, the roughness values found did not present sufficient degradation to harbor bacteria. The surface roughness of all tested materials was increased by pH-cycling. The use of materials capable of resisting degradation in the oral environment is recommended, in order to conserve their integrity and of the surrounding tissues.


Subject(s)
Acrylic Resins/chemistry , Cariogenic Agents/chemistry , Dental Bonding/methods , Resin Cements/chemistry , Aluminum Silicates/chemistry , Reference Values , Surface Properties , Materials Testing , Analysis of Variance , Statistics, Nonparametric , Microscopy, Atomic Force , Imaging, Three-Dimensional , Hydrogen-Ion Concentration
10.
Rev. Ciênc. Méd. Biol. (Impr.) ; 17(3): 315-321, nov 19, 2018. tab, ilus
Article in Portuguese | LILACS | ID: biblio-1247693

ABSTRACT

Introdução: a candidíase é uma infecção fúngica oportunista, causada pela proliferação e disseminação de espécies de Candida, que pode acometer a cavidade oral. Dentre os antifúngicos mais utilizados e de uso tópico, a nistatina é considerada o medicamento de primeira escolha. Objetivo: avaliar as propriedades físico-químicas de diferentes marcas de nistatina disponíveis no mercado, incluindo o pH, a acidez total titulável (ATT) e a determinação de sólidos solúveis totais (SST). Metodologia: trata-se de um estudo experimental in vitro, constituído por uma amostra de oito diferentes marcas de nistatina em suspensão oral de uso tópico. Foi analisado o potencial erosivo e cariogênico dessas soluções mediante a determinação de pH, ATT e SST (°Brix). Resultados: no tocante ao pH, verificou-se que a média obtida foi de 6,05 (± 0,66). Dois dos medicamentos analisados (marcas A e H) apresentaram pH abaixo do crítico para a dissolução do esmalte dental. Quanto à ATT das soluções, os valores variaram de 1,9 a 14,53 mL para atingir o pH neutro, indicando que as marcas B, C e E podem levar mais tempo para ser neutralizadas em razão da quantidade de solução necessária. A análise do °Brix revelou que a marca H apresentou o maior teor de açúcares em sua composição (44,9%). Conclusão: a formulação de nistatina da marca H apresentou pH endógeno mais crítico e percentual de sólidos solúveis totais elevado, sendo, portanto, a medicação com maior fator de risco para o desenvolvimento de cárie e erosão dentária, devendo ser consideradas as doses e frequências de uso, bem como os hábitos de higiene oral do paciente


Introduction: candidiasis is an opportunistic fungal infection caused by the proliferation and spread of Candida species that can affect the oral cavity. Among the most commonly used topical antifungal agents, nystatin is considered the first choice drug. Methodology: to evaluate the physical and chemical properties of different brands of nystatin available in the market, including pH, titratable acidity and determination of total soluble solids. Results: Regarding pH, it was verified that the mean obtained was 6.05 (± 0.66). Two of the analyzed drugs (A and H) presented pH below that considered critical for the dissolution of dental enamel. As for the titratable total acidity of the solutions, values ranged from 1.9 to 14.53 mL to reach neutral pH, indicating that the B, C and E marks may take longer to neutralize because of the amount of solution required. The analysis of ° Brix revealed that the H mark had the highest sugar content in its composition (44.9%). Conclusion: Nystatin brand H presented the worst indices in terms of endogenous pH and total sugar percentage, being therefore the medication with the highest risk factor for the development of caries and dental erosion.


Subject(s)
Tooth Erosion/chemically induced , Candidiasis, Oral/drug therapy , Cariogenic Agents/analysis , Nystatin/adverse effects , Antifungal Agents/adverse effects
11.
Rev. Ciênc. Méd. Biol. (Impr.) ; 17(3): 386-391, nov 19, 2018. tab, ilus
Article in Portuguese | LILACS | ID: biblio-1248136

ABSTRACT

Introdução: o tratamento com bochechos de dexametasona elixir é bastante descrito na literatura para casos de lesões erosivas e ulceradas em mucosa bucal. Excipientes acrescentados aos medicamentos os tornam mais palatáveis e estáveis, embora possam resultar em efeitos adversos que comprometem a saúde bucal. Objetivo: este estudo propôs-se a avaliar, in vitro, o pH, a acidez total titulável (ATT) e o teor de sólidos solúveis totais (SST) de diferentes marcas de dexametasona elixir disponíveis no mercado da cidade de Salvador, correlacionando-os ao potencial erosivo e cariogênico do medicamento para os dentes. Metodologia: seis marcas (A, B, C, D, E e F) de laboratórios distintos foram incluídas neste estudo. O valor do pH foi aferido utilizando-se pHmetro e agitador magnético; a ATT foi determinada adicionando-se hidróxido de sódio (NaOH) 0,1 N e a aferição do SST foi através de refratômetro. Os dados foram expressos em valores médios e desvios padrão. Resultados: o pH de todas as marcas investigadas apresentou medidas abaixo de 5,5, logo, todas apresentaram potencial erosivo. Na avaliação da ATT, maior volume de NaOH 0,1N foi necessário pela marca D para alcançar pH 5,5 e 7,0. Dentre as marcas investigadas, a marca B foi a que apresentou maior teor de SST em sua composição. Conclusão: soluções para uso local de dexametasona elixir possuem potencial erosivo e alto teor de SST, tornando-se, então, importante a orientação de instrução de higiene oral dos pacientes que possuem maior risco de desenvolver alterações dentárias.


Introduction: treatment with mouthwash dexamethasone elixir is sufficiently described in the literature for cases of erosive and ulcerated lesions in oral mucosa. Excipients added to drugs make them more palatable and stable, however, may result in adverse effects that compromise the health of the oral cavity. Aim: this study sets out to evaluate in vitro pH, titratable total acidity (TTA) and the total soluble solids content (TSS) of different brands of dexamethasone elixir available on the market of Salvador city, correlating them to the potential of cariogenic and erosive medicine for the dental units. Methodology: six distinct laboratories brands (A, B, C, D, E and F) were included in this study. The pH value was assessed using a pH meter and magnetic stirrer, the TTA was determined by adding sodium hydroxide (NaOH) 0.1 N and measurement of TSS was made with refractometer. The data were expressed as average and standard deviations. Results: the pH of all brands investigated presented measures below 5.5, so, all presented erosive potential. In TTA, greater volume of NaOH 0, 1N was required by D brand to achieve pH 5.5 and 7.0. Among the brands investigated, brand B was the one that presented the highest content of TSS in its composition. Conclusion: solutions for local use of dexamethasone elixir have erosive potential and high content of TSS, becoming so important the guidance of oral hygiene instruction of patients who have higher risk of developing dental changes


Subject(s)
Tooth Erosion/chemically induced , Dexamethasone/adverse effects , Cariogenic Agents/analysis , Anti-Inflammatory Agents/adverse effects , Acidity/analysis , Hydrogen-Ion Concentration
12.
Braz. oral res. (Online) ; 32: e109, 2018. tab, graf
Article in English | LILACS | ID: biblio-974435

ABSTRACT

Abstract This study investigated the effect of human milk, alone and associated with sucrose, in the cariogenicity of biofilms in a microcosm biofilm model and compared with the cariogenicity of sucrose and bovine milk. Microcosm biofilms were grown in enamel discs in 24-well plates. Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s. After 5 days, the outcome variables surface hardness change (%SHC), microbiological composition of biofilms, and pH of supernatant were analyzed. All groups had significantly lower hardness loss compared to the DMM group with 1% of sucrose. Human and bovine milk associated with sucrose showed higher hardness loss. The supernatant pH values after 6 hours of different treatments were similar for the groups sucrose and human milk associated with sucrose (p>0.05). After 18 hours at rest in pure DMM, an increase in the pH of the supernatant was observed. Higher values of total microorganisms count were found for sucrose and bovine milk groups compared to the group supplemented only by DMM. Bovine milk group showed greater amount of total aciduric microorganisms in comparison to human milk group. Within the limits of this study, it can be infered that both human and cow milks have some cariogenic potential, although differing from sucrose in terms of mineral loss.


Subject(s)
Humans , Animals , Cattle , Sucrose/adverse effects , Cariogenic Agents/adverse effects , Biofilms/growth & development , Dental Caries/microbiology , Dental Enamel/microbiology , Milk, Human/microbiology , Reference Values , Saliva/microbiology , Sucrose/chemistry , Surface Properties , Time Factors , Breast Feeding/adverse effects , Colony Count, Microbial , Cariogenic Agents/chemistry , Risk Factors , Analysis of Variance , Milk/microbiology , Diet, Cariogenic/adverse effects , Hardness Tests , Hydrogen-Ion Concentration , Milk, Human/chemistry
13.
Hig. aliment ; 31(274/275): 120-24, 30/12/2017. tab
Article in Portuguese | LILACS | ID: biblio-880368

ABSTRACT

A dieta, quando realizada de forma inadequada, passa a ser um fator determinante para a ocorrência de cárie e erosão dentária, principalmente levando em consideração o aumento do consumo de alimentos e bebidas industrializadas por crianças e adolescentes. No presente estudo avaliaram- -se as propriedades bioquímicas dos leites fermentados encontrados em um supermercado da zona leste de Teresina, PI. Foram analisadas 36 amostras de 6 marcas comerciais diferentes, por meio das propriedades de pH endógeno e Sólidos Solúveis Totais (SST). As análises foram submetidas ao teste Kolmogorov-Smirnov e a análise da variância pela ANOVA, seguida de pós-teste Tukey para a comparação entre os grupos, no programa Minitab 16. As marcas analisadas apresentaram valor de pH que variaram de 3,13 a 3,68. Todas apresentaram valores de pH abaixo do valor considerado crítico para o processo de desmineralização do esmalte (5,5). Em relação aos sólidos solúveis totais, os valores oscilaram entre 5,93 e 11,2. Com os dados obtidos, pode-se sugerir que, se essas bebidas forem ingeridas com frequência, podem apresentar tanto um potencial cariogênico como erosivo. É de fundamental importância que os profissionais de saúde estejam cientes e alertem seus pacientes sobre os riscos que esses alimentos podem oferecer à saúde bucal dos mesmos.(AU)


Diet, when performed in inadequate ways, becomes a determining factor for occurrence of caries and dental erosion, mainly when taking into consideration the increasing consumption of industrialized foods and beverages by children and adolescents. This study aimed to evaluate the biochemical properties of fermented milks found in a supermarket in the eastern zone of Teresina-PI. Thirty-six samples from six different commercial brands were analyzed through their endogenous pH and Total Soluble Solids (TSS) properties. To analyze the data, Kolmogorov-Smirnov test and analysis of variance by ANOVA, followed by a Tukey post-test for comparison between the groups in the Minitab 16 program were performed. The analyzed brands presented pH values ranging from 3.13 to 3.68. All of them presented pH values below those considered critical for the enamel (5,5). The TSS data ranged from 5.93 to 11.2. With the data obtained, it can be suggested that if those drinks are frequently ingested, it can present both cariogenic and erosive potential. It is important that health professionals be aware and alert their patients about the risks that those sort of beverage can offer to their oral health.


Subject(s)
Humans , Child , Adolescent , Tooth Erosion/etiology , Cariogenic Agents/analysis , Dental Caries/etiology , Cultured Milk Products/adverse effects , Feeding Behavior , Food Samples , Industrialized Foods
14.
Int. j. odontostomatol. (Print) ; 11(1): 107-112, abr. 2017. ilus
Article in English | LILACS | ID: biblio-841025

ABSTRACT

The aim of this in vitro study was to assess the root dentin demineralization caused by a microcosm biofilm model that has been exposed to sucrose in different ways. Materials and Methods: Saliva of two volunteers was inoculated into an artificial medium for biofilm growth and dentin blocks were immersed into these media. Dentin specimens were randomly exposed to one of the five experimental conditions: C (control group - no saliva inoculum or sucrose), 0S (saliva inoculum without sucrose, negative control), 3S (three daily one-minute immersions in 20 % sucrose), 6S (six daily one-minute immersions in 20 % sucrose), and CS (continuously immersed in 5 % sucrose). After five days, biofilm was collected to determine the concentration of intracellular and extracellular polysaccharides and the dentin surface hardness loss (SHL) was measured. The experiment was carried out in triplicate. Results: The dentin SHL was higher in groups that were exposed to sucrose (3S, 6S and CS) and there was a statistically significant difference between all groups (p<0.001). CS had higher concentrations of polysaccharides (p>0.001) and there was no statistically significant difference between the other groups (0S, 3S and 6S) (p>0.005). Conclusion: The microcosm biofilm model developed has the potential to produce root dentin demineralization at different exposures to sucrose.


El objetivo de esta investigación in vitro fue evaluar la desmineralización de la dentina radicular causada por un modelo de biofilm microcosmo que fue expuesto de diferentes maneras a la sacarosa. La saliva de dos voluntarios fue colocada en un medio artificial para crecimiento del biofilm y los bloques de dentina fueron sumergidos en estos medios. Al aza rlos bloques fueron expuestos a una de las cinco condiciones experimentales: C (grupo control ­ sin inoculación de saliva o sacarosa), 0S (inoculación de saliva sin sacarosa, control negativo), 3S (tres inmersiones diarias de un minuto en sacarosa a 20 %), 6S (seis inmersiones diarias de un minuto en sacarosa a 20 %), y CS (sumergidos continuamente en 5 % de sacarosa). Después de cinco días, el biofilm fue recogido para determinar la concentración de polisacáridos intracelulares y extracelulares y fue medida la pérdida de dureza superficial de la dentina (SHL). El experimento se repitió en tres ocasiones. La dentina SHL era mayor en los grupos que fueron expuestos a la sacarosa (3S, 6S E CS) y hubo diferencia estadísticamente significativa entre todos los grupos (P<0,001). CS presentó mayor concentración de polisacáridos (p<0,001) y no hubo diferencia estadísticamente significativa entre los demás grupos (0S, 3S E 6S) (p>0,005). El modelo del biofilm desarrollado tiene potencial para producir desmineralización de la dentina radicular en diferentes exposiciones a la sacarosa.


Subject(s)
Humans , Cariogenic Agents/pharmacology , Dentin/drug effects , Sucrose/pharmacology , Tooth Demineralization , Biofilms/drug effects , In Vitro Techniques , Polysaccharides/analysis , Saliva/chemistry
15.
Rev. odontol. UNESP (Online) ; 45(3): 149-153, tab
Article in Portuguese | LILACS, BBO | ID: lil-785878

ABSTRACT

Introdução: A espécie Psidium cattleianum Sabine tem despertado o interesse dos pesquisadores por apresentar, além de atividade cicatrizante, analgésica e antioxidante, propriedades antimicrobianas frente a micro-organismos da mucosa oral que podem atuar como agentes cariogênicos. Objetivo: Foi avaliada, neste trabalho, a atividade antimicrobiana de extratos bruto e fracionados das folhas do araçá, além do seu perfil cromatográfico. Material e método: Para avaliação da atividade antimicrobiana, foi utilizada a técnica de microdiluição, para determinação da Concentração Inibitória Mínima (CIM), e repique do inóculo em Ágar Muller Hinton (Himedia), para averiguação da Concentração Bactericida Mínima (CBM). O extrato foi testado nas concentrações entre 10 e 500 µg/mL. O perfil cromatográfico foi realizado pelo método de Cromatografia Líquida de Alta Eficiência (CLAE). Resultado: Os resultados obtidos foram submetidos a uma análise descritiva e foi possível observar a atividade inibitória dos extratos do P. cattleianum contra S. mutans e S. oralis. Conclusão: A atividade antimicrobiana dos extratos de P. cattleianum contra micro-organismos orais justifica maiores estudos para a utilização medicinal dessa espécie, como, por exemplo, sua utilização em enxaguantes bucais.


Introduction: The specie Psidium cattleianum Sabine has aroused the interest of researchers to exhibit, healing activity, analgesic, antioxidant, antimicrobial properties against the micro-organisms of the oral mucosa that can act as cariogenic agents. Objective: It was analyzed in this study the antimicrobial activity of crude extracts and fractions and chromatographic profile of araçá. Material and method: For antimicrobial activity, we used the technique of on broth microdilution for determining the Minimum Inhibitory Concentration (MIC) and replating of inoculum in Muller Hinton (Himedia) agar for finding the Minimum Bactericidal Concentration (MBC). The extract was tested at concentrations between 10 and 500 µg/mL. The chromatographic profile was performed by the method of High Performance Liquid Chromatography. Result: The results were submitted to a descriptive analysis was possible to observe the inhibitory activity of the extracts of P. cattleianum against S. oralis and S. mutans. Conclusion: The antimicrobial activity of P. cattleianum extracts against oral microorganisms justifies the use of this species as a medicinal plant.


Subject(s)
Plants, Medicinal , Streptococcus mutans , In Vitro Techniques , Cariogenic Agents , Mouth Mucosa , Wound Healing , Analgesia , Antioxidants
16.
Braz. oral res. (Online) ; 30(1): e52, 2016. tab, graf
Article in English | LILACS | ID: biblio-952032

ABSTRACT

Abstract Sucrose is the most cariogenic dietary carbohydrate and starch is considered non-cariogenic for enamel and moderately cariogenic for dentine. However, the cariogenicity of the combination of starch and sucrose remains unclear. The aim of this study was to evaluate the effect of this combination on Streptococcus mutans biofilm composition and enamel and dentine demineralization. Biofilms of S. mutans UA159 were grown on saliva-coated enamel and dentine slabs in culture medium containing 10% saliva. They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose). To simulate the effect of human salivary amylase on the starch metabolization, the biofilms were pretreated with saliva before each treatment and saliva was also added to the culture medium. Acidogenicity of the biofilm was estimated by evaluating (2 times/day) the culture medium pH. After 4 (dentine) or 5 (enamel) days of growth, biofilms (n = 9) were individually collected, and the biomass, viable microorganism count, and polysaccharide content were quantified. Dentine and enamel demineralization was assessed by determining the percentage of surface hardness loss. Biofilms exposed to starch + sucrose were more acidogenic and caused higher demineralization (p < 0.0001) on either enamel or dentine than those exposed to each carbohydrate alone. The findings suggest that starch increases the cariogenic potential of sucrose.


Subject(s)
Humans , Animals , Cattle , Young Adult , Starch/chemistry , Cariogenic Agents/chemistry , Tooth Demineralization/etiology , Dietary Sucrose/chemistry , Dental Enamel/chemistry , Dentin/chemistry , Reference Values , Saliva/microbiology , Saliva/chemistry , Streptococcus mutans/growth & development , Time Factors , Colony Count, Microbial , Tooth Demineralization/microbiology , Statistics, Nonparametric , Biofilms/growth & development , Dental Enamel/microbiology , Dentin/microbiology
17.
J. appl. oral sci ; 22(1): 68-72, Jan-Feb/2014. tab
Article in English | LILACS, BBO | ID: lil-699921

ABSTRACT

Objective: The oral environment is subject to biofilm accumulation and cariogenic challenge, and few studies exist on the effect of these factors on the bond strength of adhesive systems. The aim of this study was to test if the exposure of adhesive interfaces to cariogenic challenge under biofilm accumulation could promote higher degradation than the exposure to biofilm accumulation alone. Material And Methods: Five molars were ground until exposure of medium dentin and then restored (Single Bond 2 and Z250 3M ESPE). The tooth/resin sets were cut to obtain beam-shaped specimens, which were distributed according to the aging conditions (n=20): water for 24 h (control); biofilm under cariogenic challenge for 3, 5 or 10 days; biofilm without cariogenic challenge for 10 days; and water for 3 months. Microcosm biofilms were formed from human saliva and grown in a saliva analogue medium, supplemented or not with sucrose to promote cariogenic challenge. Specimens were tested for microtensile bond strength, and failure modes were classified using light microscopy. Bond strength data were analyzed using ANOVA and failure modes were analyzed using ANOVA on ranks (α=0.05). Results: No significant differences in bond strength were detected among the aging methods (P=0.248). The aging period was associated with an increase in the frequency of adhesive failures for the groups aged for 10 days or longer (P<0.001). Conclusion: Aging leads to a higher prevalence of interfacial adhesive failures, although this effect is not associated with cariogenic challenge or reduction in bond strengths. .


Subject(s)
Humans , Biofilms/drug effects , Dental Caries/microbiology , Dentin , Dentin-Bonding Agents/chemistry , Analysis of Variance , Bisphenol A-Glycidyl Methacrylate/chemistry , Cariogenic Agents/pharmacology , Dental Restoration Failure , Dentin/drug effects , Dentin/microbiology , Random Allocation , Saliva/microbiology , Sucrose/pharmacology , Surface Properties , Sweetening Agents/pharmacology , Tensile Strength , Time Factors , Water/chemistry
18.
International Journal of Oral Science ; (4): 195-204, 2014.
Article in English | WPRIM | ID: wpr-358128

ABSTRACT

The objective of the study was to analyse Streptococcus mutans biofilms grown under different dietary conditions by using multifaceted methodological approaches to gain deeper insight into the cariogenic impact of carbohydrates. S. mutans biofilms were generated during a period of 24 h in the following media: Schaedler broth as a control medium containing endogenous glucose, Schaedler broth with an additional 5% sucrose, and Schaedler broth supplemented with 1% xylitol. The confocal laser scanning microscopy (CLSM)-based analyses of the microbial vitality, respiratory activity (5-cyano-2,3-ditolyl tetrazolium chloride, CTC) and production of extracellular polysaccharides (EPS) were performed separately in the inner, middle and outer biofilm layers. In addition to the microbiological sample testing, the glucose/sucrose consumption of the biofilm bacteria was quantified, and the expression of glucosyltransferases and other biofilm-associated genes was investigated. Xylitol exposure did not inhibit the viability of S. mutans biofilms, as monitored by the following experimental parameters: culture growth, vitality, CTC activity and EPS production. However, xylitol exposure caused a difference in gene expression compared to the control. GtfC was upregulated only in the presence of xylitol. Under xylitol exposure, gtfB was upregulated by a factor of 6, while under sucrose exposure, it was upregulated by a factor of three. Compared with glucose and xylitol, sucrose increased cell vitality in all biofilm layers. In all nutrient media, the intrinsic glucose was almost completely consumed by the cells of the S. mutans biofilm within 24 h. After 24 h of biofilm formation, the multiparametric measurements showed that xylitol in the presence of glucose caused predominantly genotypic differences but did not induce metabolic differences compared to the control. Thus, the availability of dietary carbohydrates in either a pure or combined form seems to affect the cariogenic potential of S. mutans biofilms.


Subject(s)
Humans , Bacterial Load , Bacteriological Techniques , Biofilms , Cariogenic Agents , Metabolism , Pharmacology , Culture Media , Dental Enamel , Microbiology , Fluorescent Dyes , Gene Expression Regulation, Bacterial , Gene Expression Regulation, Enzymologic , Genotype , Glucose , Metabolism , Glucosyltransferases , Metabolism , Microbial Viability , Microscopy, Confocal , Polysaccharides, Bacterial , Streptococcus mutans , Metabolism , Sucrose , Metabolism , Pharmacology , Sweetening Agents , Metabolism , Pharmacology , Tetrazolium Salts , Time Factors , Up-Regulation , Xylitol , Metabolism , Pharmacology
19.
Article in Spanish | LILACS | ID: lil-777806

ABSTRACT

La caries es una enfermedad infecciosa y contagiosa que por tener un a alta prevalencia a nivel mundial afectando tanto a niños como adultos, es necesario buscar y evaluar nuevas alternativas de tratamiento y prevención, que permitan mejorar la salud bucal de toda la población afectada, en donde el profesional debe estar involucrado.En cuanto a los métodos preventivos se encuentran los barnices con fluoruros y los derivados de la caseína, que se hallan en los productos lácteos, dentífricos y chicles, siendo de gran utilidad para pacientes pediátricos.Aunado a ello, se están utilizando actualmente, diversos métodos para la eliminación de la caries dental que permiten realizar procedimientos poco invasivos y que pueden ser aplicados en pacientes pediátricos o con discapacidad debido a que no requieren la utilización de anestesia local, ni instrumental rotatorio, mejorando la adaptación a la consulta y además obteniendo resultados favorables con respecto a las técnicas convencionales.Los diferentes procedimientos pueden ser mecánicos, químicos-mecánicos y de otros tipos como el láser que en conjunto conforman los métodos no invasivos. Ellos permiten una amplia variedad de opciones dependiendo de la población a la cual vayan dirigidos, tomando en cuenta el acceso a servicios de salud, estatus socio-económico, edad y requerimientos a corto plazo para evitar la extensión de las lesiones que implica perdidas tempranas de estructuras dentales que afectan la salud integral del individuo.


Dental caries is an infectious and contagious disease with an elevated prevalence in children and adults around the world. For that reason it is necessary to evaluate the new methods of prevention and treatment to improve bucal health in this population.In relation to the preventive methods, there are the fluoride varnish and casein derivates that can be in the milk, the tooth pastes and chewing gum and can be use to improve children´s bucal health.Nowadays there are many new methods that are using to eliminate caries in a non-invasive way. Many of them do not need the use of local anesthesia or rotatory handpieces and represent a good alternative to the behavibucal management of pediatric patients.The several methods can be mechanical, chemical & mechanical and others like laser that constitute the non-invasive methods. They give to the professional many options depending to some factors of the patients like age, social and economical status, possibility to access to health services and other requirements to avoid the extension of the caries lesions and the dental structure loss.


Subject(s)
Humans , Male , Female , Child, Preschool , Child , Dental Caries/complications , Dental Caries , Dental Caries/therapy , Fluorides, Topical/administration & dosage , Fluorides, Topical/therapeutic use , Oral Health , Cariogenic Agents , Caseins , Dental Cavity Lining , Pediatric Dentistry
20.
Acta odontol. venez ; 52(1)2014. tab
Article in Spanish | LILACS | ID: lil-777819

ABSTRACT

Cuando se considera a la caries como una enfermedad, no como una lesión, y se identifican los factores de riesgo implicados en su etiología, se poseen las herramientas para prevenir la aparición de nuevas lesiones y la progresión de las existentes. Esto implica un cambio de conducta en los profesionales de la salud bucal, tanto a nivel académico en las escuelas de odontología, como en los servicios públicos y privados. Es por ello, que se hace necesario el diseño, evaluación, implementación y divulgación de mecanismos e instrumentos que faciliten el diagnóstico del riesgo a caries. Asignar al paciente en un nivel de riesgo es el primer paso para el manejo y tratamiento de la enfermedad caries. Esta evaluación se realiza en dos fases: en la primera se identifican factores patológicos específicos o indicadores de la enfermedad, factores biológicos predisponentes y factores protectores de cada paciente. En la segunda fase se determina el nivel de riesgo de acuerdo al análisis de todos los factores involucrados, y posteriormente se planifica, desarrolla y ejecuta estrategias personalizadas dirigidas a controlar la enfermedad.


When considering caries as a disease, not a lesion, and identifying the risk factors involved in their causes, you have the tools to prevent the appearance of new lesions and progression of existing ones. This implies a change in behavior in oral health professionals, both academic dental schools, and in public and private services. This is why it is necessary to do the design, evaluation, implementation and dissemination of mechanisms and tools to facilitate the diagnosis of caries risk. Assign the patient to a level of risk is the first step in the management and treatment of caries disease. This evaluation is performed in two phases: in the first phase are identified pathological factors specific or indicators of the disease, biological factors predisposing and protective factors of each patient. The second phase determines the level of risk according to the analysis of all the factors involved, and then plans, develops and implements customized strategies aimed at controlling the disease.


Subject(s)
Humans , Male , Female , Dental Caries/diagnosis , Dental Caries/etiology , Dental Caries/prevention & control , Oral Hygiene/methods , Process Assessment, Health Care , Biological Factors , Cariogenic Agents , Dental Devices, Home Care , Oral Health
SELECTION OF CITATIONS
SEARCH DETAIL