Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 264
Filter
1.
Biol. Res ; 57: 1-1, 2024. ilus, graf
Article in English | LILACS | ID: biblio-1550056

ABSTRACT

BACKGROUND: Tumor-derived small extracellular vesicles (sEVs) can promote tumorigenic and metastatic capacities in less aggressive recipient cells mainly through the biomolecules in their cargo. However, despite recent advances, the specific molecules orchestrating these changes are not completely defined. Lactadherin is a secreted 0protein typically found in the milk fat globule membrane. Its overexpression has been associated with increased tumorigenesis and metastasis in breast cancer (BC) and other tumors. However, neither its presence in sEVs secreted by BC cells, nor its role in sEV-mediated intercellular communication have been described. The present study focused on the role of lactadherin-containing sEVs from metastatic MDA-MB-231 triple-negative BC (TNBC) cells (sEV-MDA231) in the promotion of pro-metastatic capacities in non-tumorigenic and non-metastatic recipient cells in vitro, as well as their pro-metastatic role in a murine model of peritoneal carcinomatosis. RESULTS: We show that lactadherin is present in sEVs secreted by BC cells and it is higher in sEV-MDA231 compared with the other BC cell-secreted sEVs measured through ELISA. Incubation of non-metastatic recipient cells with sEV- MDA231 increases their migration and, to some extent, their tumoroid formation capacity but not their anchorage-independent growth. Remarkably, lactadherin blockade in sEV-MDA231 results in a significant decrease of those sEV-mediated changes in vitro. Similarly, intraperitoneally treatment of mice with MDA-MB-231 BC cells and sEV-MDA231 greatly increase the formation of malignant ascites and tumor micronodules, effects that were significantly inhibited when lactadherin was previously blocked in those sEV-MDA231. CONCLUSIONS: As to our knowledge, our study provides the first evidence on the role of lactadherin in metastatic BC cell-secreted sEVs as promoter of: (i) metastatic capacities in less aggressive recipient cells, and ii) the formation of malignant ascites and metastatic tumor nodules. These results increase our understanding on the role of lactadherin in sEVs as promoter of metastatic capacities which can be used as a therapeutic option for BC and other malignancies.


Subject(s)
Humans , Animals , Mice , Ascites , Extracellular Vesicles , Biological Transport , Cell Communication , Cell Line, Tumor , Carcinogenesis
3.
Journal of Zhejiang University. Science. B ; (12): 1-14, 2023.
Article in English | WPRIM | ID: wpr-971465

ABSTRACT

Skeletal muscle plays a paramount role in physical activity, metabolism, and energy balance, while its homeostasis is being challenged by multiple unfavorable factors such as injury, aging, or obesity. Exosomes, a subset of extracellular vesicles, are now recognized as essential mediators of intercellular communication, holding great clinical potential in the treatment of skeletal muscle diseases. Herein, we outline the recent research progress in exosomal isolation, characterization, and mechanism of action, and emphatically discuss current advances in exosomes derived from multiple organs and tissues, and engineered exosomes regarding the regulation of physiological and pathological development of skeletal muscle. These remarkable advances expand our understanding of myogenesis and muscle diseases. Meanwhile, the engineered exosome, as an endogenous nanocarrier combined with advanced design methodologies of biomolecules, will help to open up innovative therapeutic perspectives for the treatment of muscle diseases.


Subject(s)
Exosomes/physiology , Muscle, Skeletal/metabolism , Cell Communication , Homeostasis
4.
Chinese Journal of Stomatology ; (12): 86-91, 2023.
Article in Chinese | WPRIM | ID: wpr-970760

ABSTRACT

Tunneling nanotube (TNT) is a newly discovered communication mode between animal cells in recent years, which have important physiological and pathological significance. However, the role of TNT in bone biology is still unclear. At present, there are many reports about tunneling nanotubes in bone marrow mesenchymal stem cells, osteoclast precursor cells, osteoblasts and immune cells. This review describes the research advances of TNT and its research progress in bone biology. It looks forward to the research direction of TNT in oral and maxillofacial bone development and bone biology, to provide new strategies for the maintenance of bone homeostasis and the treatment of bone diseases.


Subject(s)
Animals , Bone and Bones , Nanotubes , Osteoclasts , Biology , Cell Communication/physiology
5.
Chinese Medical Journal ; (24): 194-206, 2023.
Article in English | WPRIM | ID: wpr-970054

ABSTRACT

BACKGROUND@#Imatinib mesylate (IM) resistance is an emerging problem for chronic myeloid leukemia (CML). Previous studies found that connexin 43 (Cx43) deficiency in the hematopoietic microenvironment (HM) protects minimal residual disease (MRD), but the mechanism remains unknown.@*METHODS@#Immunohistochemistry assays were employed to compare the expression of Cx43 and hypoxia-inducible factor 1α (HIF-1α) in bone marrow (BM) biopsies of CML patients and healthy donors. A coculture system of K562 cells and several Cx43-modified bone marrow stromal cells (BMSCs) was established under IM treatment. Proliferation, cell cycle, apoptosis, and other indicators of K562 cells in different groups were detected to investigate the function and possible mechanism of Cx43. We assessed the Ca 2+ -related pathway by Western blotting. Tumor-bearing models were also established to validate the causal role of Cx43 in reversing IM resistance.@*RESULTS@#Low levels of Cx43 in BMs were observed in CML patients, and Cx43 expression was negatively correlated with HIF-1α. We also observed that K562 cells cocultured with BMSCs transfected with adenovirus-short hairpin RNA of Cx43 (BMSCs-shCx43) had a lower apoptosis rate and that their cell cycle was blocked in G0/G1 phase, while the result was the opposite in the Cx43-overexpression setting. Cx43 mediates gap junction intercellular communication (GJIC) through direct contact, and Ca 2+ is the key factor mediating the downstream apoptotic pathway. In animal experiments, mice bearing K562, and BMSCs-Cx43 had the smallest tumor volume and spleen, which was consistent with the in vitro experiments.@*CONCLUSIONS@#Cx43 deficiency exists in CML patients, promoting the generation of MRD and inducing drug resistance. Enhancing Cx43 expression and GJIC function in the HM may be a novel strategy to reverse drug resistance and promote IM efficacy.


Subject(s)
Animals , Humans , Mice , Apoptosis , Bone Marrow Cells , Cell Communication , Connexin 43/genetics , Gap Junctions/metabolism , Imatinib Mesylate/therapeutic use , K562 Cells , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/pathology , Mesenchymal Stem Cells/metabolism , Tumor Microenvironment , Calcium/metabolism
6.
Protein & Cell ; (12): 238-261, 2023.
Article in English | WPRIM | ID: wpr-982533

ABSTRACT

Neurons migrate from their birthplaces to the destinations, and extending axons navigate to their synaptic targets by sensing various extracellular cues in spatiotemporally controlled manners. These evolutionally conserved guidance cues and their receptors regulate multiple aspects of neural development to establish the highly complex nervous system by mediating both short- and long-range cell-cell communications. Neuronal guidance genes (encoding cues, receptors, or downstream signal transducers) are critical not only for development of the nervous system but also for synaptic maintenance, remodeling, and function in the adult brain. One emerging theme is the combinatorial and complementary functions of relatively limited classes of neuronal guidance genes in multiple processes, including neuronal migration, axonal guidance, synaptogenesis, and circuit formation. Importantly, neuronal guidance genes also regulate cell migration and cell-cell communications outside the nervous system. We are just beginning to understand how cells integrate multiple guidance and adhesion signaling inputs to determine overall cellular/subcellular behavior and how aberrant guidance signaling in various cell types contributes to diverse human diseases, ranging from developmental, neuropsychiatric, and neurodegenerative disorders to cancer metastasis. We review classic studies and recent advances in understanding signaling mechanisms of the guidance genes as well as their roles in human diseases. Furthermore, we discuss the remaining challenges and therapeutic potentials of modulating neuronal guidance pathways in neural repair.


Subject(s)
Humans , Axon Guidance/genetics , Neurons , Axons/metabolism , Signal Transduction/genetics , Cell Communication
7.
Psicol. ciênc. prof ; 43: e255712, 2023.
Article in Portuguese | LILACS, INDEXPSI | ID: biblio-1529208

ABSTRACT

Com o advento da covid-19, foi declarado estado de emergência de saúde pública e decretadas medidas de isolamento e distanciamento social para conter a propagação da doença. O Conselho Federal de Psicologia, considerando a importância do acolhimento seguro durante a pandemia, publicou a Resolução CFP nº 4/2020, permitindo que serviços psicológicos aconteçam de maneira remota. O presente estudo visa, através do Método da Cartografia, apresentar a construção de um setting on-line para intervenções grupais e os desafios na oferta de acolhimento e atendimento remoto. Foram ofertados grupos terapêuticos, por meio da plataforma Google Meet, para estudantes da Universidade Federal Rural do Rio de Janeiro. Um diário de bordo foi produzido para acompanhar as forças que atravessavam e constituíam o território e a experiência grupal remota. Compreendemos que o território-espaço-grupal-on-line era composto pelo espaço virtual em que nos reuníamos, pelos espaços individuais de cada integrante e pelas forças que os atravessavam. Observamos que nem sempre os participantes dispunham de um lugar privado, mas estiveram presentes no encontro com câmeras e áudios abertos e/ou fechados e/ou através do chat da videochamada. A participação no grupo funcionou como alternativa no momento de distanciamento social, sendo uma possibilidade para o atendimento psicológico em situações de dificuldade de encontros presenciais; entretanto, se mostrou dificultada em diversos momentos, pela falta de equipamentos adequados e instabilidade na internet, fatores que interferiram nas reuniões e impactaram na possibilidade de falar e escutar o que era desejado.(AU)


With the advent of COVID-19, a state of public health was declared, and measures of isolation and social distance to contain the spread of the disease was decreed. The Federal Council of Psychology, considering the importance of safe reception during the pandemic, published CFP Resolution No. 4/2020, allowing psychological services to happen remotely. This study narrates, via the Cartography Method, the experience of inventing an Online Setting for group reception. Therapeutic groups were offered, via Google Meet Platform, to students at the Federal Rural University of Rio de Janeiro. A logbook was produced to accompany the forces that crossed and constituted the territory and the remote group experience. We understand that the territoryspace-group-online was composed by the virtual-space that we gathered, by the individualspaces of each member and by the forces that crossed them. We observed that the participants did not always have a private place, but they were present at the meeting with open and/or closed cameras and audio and/or through the video call chat. Participation in the group worked as an alternative at the time of social distancing, being a possibility for psychological care in situations of difficulty in face-to-face meetings, however, it proved to be difficult at various times, due to the lack of adequate equipment and instability on the internet, factors that interfered in meetings and impacted the possibility of speaking and listening to what was desired.(AU)


La llegada de la COVID-19 produjo un estado de emergencia de salud pública, en el que se decretaron medidas de confinamiento y distanciamiento físico para contener la propagación de la enfermedad. El Consejo Federal de Psicología, considerando la importancia de la acogida segura durante la pandemia, publicó la Resolución CFP nº 4/2020, por la que se permite la atención psicológica remota. Este estudio tiene por objetivo presentar, mediante el método de la Cartografía, la elaboración de un escenario en línea para la intervención grupal y los desafíos en la oferta de acogida y atención remota. Grupos terapéuticos se ofrecieron, en la plataforma Google Meet, a estudiantes de la Universidad Federal Rural de Río de Janeiro. Se elaboró un diario para acompañar a las fuerzas que atravesaron y constituyeron el territorio y la experiencia remota del grupo. Entendemos que el territorio-espacio-grupo-en línea estaba compuesto por el espacio-virtual que reunimos, por los espacios individuales de cada integrante y por las fuerzas que los atravesaban. Observamos que los participantes no siempre tenían un lugar privado y que estaban presentes en la reunión con cámaras y audio abiertos y/o cerrados y/o por el chat de la videollamada. La participación en el grupo funcionó como una alternativa en el momento del distanciamiento físico y revela ser una posibilidad de atención psicológica en situaciones de dificultad en los encuentros presenciales, sin embargo, se mostró difícil en varios momentos, ya sea por la falta de medios adecuados o por inestabilidad en Internet, factores que interferían en las reuniones e impactaban en la posibilidad de hablar y escuchar lo que se deseaba.(AU)


Subject(s)
Humans , Male , Female , Psychology , Attitude , Answering Services , Internet-Based Intervention , Teleworking , COVID-19 , Anxiety , Personal Satisfaction , Preceptorship , Professional Practice Location , Psychoanalysis , Psychology, Social , Quality of Life , Safety , Social Identification , Social Values , Socialization , Socioeconomic Factors , Speech , Students , Teaching , Unemployment , Universities , Work , Behavior , Behavior and Behavior Mechanisms , Work Hours , Attitude to Computers , Medical Informatics Applications , Bereavement , Single Parent , Family , Catchment Area, Health , Cell Adhesion , Cell Communication , Quarantine , Communicable Disease Control , Mental Health , Life Expectancy , Universal Precautions , Infection Control , Employment, Supported , Communication , Mandatory Testing , Confidentiality , Privacy , Imagery, Psychotherapy , Psychotherapeutic Processes , Internet , Crisis Intervention , Personal Autonomy , Death , Trust , Codes of Ethics , Depression , Air Pollution , Educational Status , Disease Prevention , Centers of Connivance and Leisure , Professional Training , Faculty , Family Relations , Fear , Emotional Intelligence , Return to Work , Hope , Social Skills , Emotional Adjustment , Optimism , Healthy Lifestyle , Work-Life Balance , Mentoring , Sadness , Respect , Solidarity , Psychological Distress , Social Integration , Transtheoretical Model , Psychosocial Intervention , Listening Effort , Social Cohesion , Belonging , Cognitive Training , Diversity, Equity, Inclusion , Psychological Well-Being , Household Work , Humanities , Individuality , Sleep Initiation and Maintenance Disorders , Interpersonal Relations , Learning , Life Change Events , Motivation , Object Attachment
8.
Einstein (Säo Paulo) ; 20: eRB5954, 2022. tab
Article in English | LILACS | ID: biblio-1364790

ABSTRACT

ABSTRACT Despite advances in understanding of carcinogenesis and of treatment of acute myeloid leukemia, this neoplasm still has a lethality of at least 30%. The search for biomarkers that can predict the response to treatment in the early stages of the disease is still necessary. In recent years, a new form of cellular communication between tumor and non-neoplastic cells has been discovered: the exchange of information through extracellular vesicles. These are small vesicles released by membrane-coated cells that carry proteins, lipids, messenger RNAs, microRNA and DNA, which can be internalized and promote biological changes in target cells. Exosomes are qualified as a type of extracellular vesicle and, in tumors, carry immunoinhibitory signals that promote the escape of immune control. Recent studies have showed their involvement in communication with the cells of the tumor microenvironment and with chemoresistance in several tumors. To date, there is no information about immunoregulatory microRNAs transported by exosomes and their correlation with clinical evolution during chemotherapy for acute myeloid leukemia. Knowledge about immunomodulatory microRNAs obtained by leukemic cells and transported by exosomes can direct us towards the design of new diagnostic and treatment tools in this type of leukemia.


Subject(s)
Humans , Leukemia, Myeloid, Acute/diagnosis , Leukemia, Myeloid, Acute/genetics , MicroRNAs/metabolism , Exosomes/genetics , Exosomes/metabolism , Biomarkers , Cell Communication , Tumor Microenvironment/genetics
9.
Acta Physiologica Sinica ; (6): 67-72, 2022.
Article in Chinese | WPRIM | ID: wpr-927582

ABSTRACT

Extracellular vesicles (EVs) are lipid bilayer-enclosed structures containing diverse bioactive cargoes that play a major role in intercellular communication in both physiological and pathological conditions. Currently, the field of EV-based therapy has been rapidly growing, and two main therapeutic uses of EVs can be surmised: (i) exploiting stem cell-derived EVs as therapeutic agents; and (ii) employing EVs as natural therapeutic vectors for drug delivery. This review will discuss the recent advances in EV-based therapy in the treatment of renal disease.


Subject(s)
Humans , Cell Communication , Drug Delivery Systems , Extracellular Vesicles , Kidney Diseases/therapy
10.
Biol. Res ; 55: 35-35, 2022. ilus
Article in English | LILACS | ID: biblio-1429901

ABSTRACT

Extracellular vesicles (EVs) are naturally released membrane vesicles that act as carriers of proteins and RNAs for intercellular communication. With various biomolecules and specific ligands, EV has represented a novel form of information transfer, which possesses extremely outstanding efficiency and specificity compared to the classical signal transduction. In addition, EV has extended the concept of signal transduction to intercellular aspect by working as the collection of extracellular information. Therefore, the functions of EVs have been extensively characterized and EVs exhibit an exciting prospect for clinical applications. However, the biogenesis of EVs and, in particular, the regulation of this process by extracellular signals, which are essential to conduct further studies and support optimal utility, remain unclear. Here, we review the current understanding of the biogenesis of EVs, focus on the regulation of this process by extracellular signals and discuss their therapeutic value.


Subject(s)
Extracellular Vesicles/metabolism , Biological Transport , RNA/metabolism , Signal Transduction , Cell Communication/physiology
11.
International Journal of Oral Science ; (4): 15-15, 2021.
Article in English | WPRIM | ID: wpr-880869

ABSTRACT

Gap junction (GJ) has been indicated to have an intimate correlation with adhesion junction. However, the direct interaction between them partially remains elusive. In the current study, we aimed to elucidate the role of N-cadherin, one of the core components in adhesion junction, in mediating connexin 43, one of the functional constituents in gap junction, via transforming growth factor-β1(TGF-β1) induction in osteoblasts. We first elucidated the expressions of N-cadherin induced by TGF-β1 and also confirmed the upregulation of Cx43, and the enhancement of functional gap junctional intercellular communication (GJIC) triggered by TGF-β1 in both primary osteoblasts and MC3T3 cell line. Colocalization analysis and Co-IP experimentation showed that N-cadherin interacts with Cx43 at the site of cell-cell contact. Knockdown of N-cadherin by siRNA interference decreased the Cx43 expression and abolished the promoting effect of TGF-β1 on Cx43. Functional GJICs in living primary osteoblasts and MC3T3 cell line were also reduced. TGF-β1-induced increase in N-cadherin and Cx43 was via Smad3 activation, whereas knockdown of Smad3 signaling by using siRNA decreased the expressions of both N-cadherin and Cx43. Overall, these data indicate the direct interactions between N-cadherin and Cx43, and reveal the intervention of adhesion junction in functional gap junction in living osteoblasts.


Subject(s)
Cadherins , Cell Communication , Connexin 43 , Osteoblasts , Transforming Growth Factor beta1
12.
Journal of Central South University(Medical Sciences) ; (12): 183-188, 2021.
Article in English | WPRIM | ID: wpr-880641

ABSTRACT

Traumatic brain injury (TBI) is a main cause of death and disability worldwide, posing a serious threat to public health. But currently, the diagnosis and treatments for TBI are still very limited. Exosomes are a group of extracellular vesicles and participate in multiple physiological processes including intercellular communication and substance transport. Non-coding RNAs (ncRNA) are of great abundancy as cargo of exosomes. Previous studies have shown that ncRNAs are involved in several pathophysiological processes of TBI. However, the concrete mechanisms involved in the effects induced by exosome-derived ncRNA remain largely unknown. As an important component of exosomes, ncRNA is of great significance for diagnosis, precise treatment, response evaluation, prognosis prediction, and complication management after TBI.


Subject(s)
Humans , Brain Injuries, Traumatic/genetics , Cell Communication , Exosomes/genetics , Extracellular Vesicles , RNA, Untranslated/genetics
13.
Journal of Experimental Hematology ; (6): 1812-1818, 2021.
Article in Chinese | WPRIM | ID: wpr-922340

ABSTRACT

OBJECTIVE@#To investigate the effect of gap junction intercellular communication (GJIC) combined by connexin43 (Cx43) and its signal to the biobehavior of multiple myeloma (MM) cells, and its possible mechanism.@*METHODS@#The mesenchymal stem cell (MSC) cells were isolated and cultured from patients with MM and normal donors. The expression of connexin43 (Cx43) in MSC cells from different sources was detected by RT-PCR and Western blot. The side population (SP) cells were sorted by flow cytometry (FCM). The effect of MSC cells from different sources to the cell cycle, Cx43 expression, colony formation in vitro, stem cell related genes expression, cytokines secretion and chemoresistance in MM SP cells as well as with or without Cx43 inhibitor 18α-glycyrrhetinic acid (18α-GA) was observed.@*RESULTS@#There was no significantly difference between the MSC isolated from normal donor and MM patients. Western blot showed that Cx43 expression in SP cells was up-regulated when the cells were incubated with MSC, and medium containing 18α-GA could partially inhibit it, moreover, it was more significant in MSC cells of MM patients. The ability of colony formation of SP cells in vitro was higher than those of MM cells and MM-MSC could promote the colony formation in a co-culture manner. The effect of MM-MSC to SP cells was down-regulated after 18α-GA was added. RT-PCR showed that there was several important stem cell-related genes including c-myc, Oct-4 Klf-4, and Sox-2 were found in RPMI 8226 cells, but those cells were up-regulated in SP cells (P0.05). Cytometry bead array assays showed that MM-MSCs could secrete high level of IL-6, but the levels of IL-6, IL-10 and TGF-β increased significantly when the MM-MSCs were co-cultured with SP cells (P<0.05), especially the levels of IL-6 and IL-10 were significantly higher than cultured alone. There was no significant change in the levels of bFGF and IL-17 before and after co-cultured. The levels of IL-6, IL-10 and TGF-β in supernatant decreased significantly after GJ inhibitor 18α-GA was added. PI/Annexin V assay showed that MM cells were sensitive to bortezomib (BTZ)-induced apoptosis, but the sensitivity for SP cells was weaker. The ratio of cell apoptosis was 75.2%±0.77% and 8.12%±0.86% (P<0.001), respectively. MM-MSC could down-regulate the cell apoptosis induced by BTZ, while the sensitivity of MM cells to BTZ could be partially recovered after GJ inhibitor was added.@*CONCLUSION@#MSC derived from MM patients can enhance GJIC to maintain its "hematopoiesis" by up-regulating the expression of Cx43 in MM cells, and at the same time promote cell proliferation and drug recistance by secreting multiple cytokines, which finally contributes to the relapse of MM.


Subject(s)
Humans , Cell Communication , Coculture Techniques , Connexin 43 , Mesenchymal Stem Cells , Multiple Myeloma
14.
Acta Physiologica Sinica ; (6): 1035-1042, 2021.
Article in Chinese | WPRIM | ID: wpr-921308

ABSTRACT

Exosomes are nanometer-sized membranous extracellular vesicles that can be secreted by almost all types of cells in the body. Exosomes are involved in cell-to-cell communication through autocrine and paracrine forms. Exosomal microRNAs (miRNAs) are stable in plasma, urine and other body fluids, and have various biological functions. They play an irreplaceable role in the occurrence, development, immune regulation of systemic lupus erythematosus (SLE). Recent studies have proposed that exosomal miRNAs have promising application prospects in the pathogenesis, early diagnosis, and treatment of SLE. Therefore, this review aims to introduce the current research progress on exosomal miRNAs in SLE and analyze their potential application value.


Subject(s)
Humans , Cell Communication , Exosomes/genetics , Lupus Erythematosus, Systemic/genetics , MicroRNAs/genetics
15.
Electron. j. biotechnol ; 43: 55-61, Jan. 2020. tab, ilus, graf
Article in English | LILACS | ID: biblio-1087522

ABSTRACT

Background: Matrix metalloproteinase 12 (MMP12), a member of MMPs, can take lots of roles including extracellular matrix component degradation, viral infection, inflammation, tissue remodeling and tumorigenesis. To explore the transcriptional regulation of MMP12 gene, a sensitive luciferase reporter HEK293 cell line for endogenous MMP12 promoter was generated by CRISPR/Cas9 technology. Results: The HEK293-MMP12-T2A-luciferase-KI cell line was successfully established by CRISPR/Cas9 technology. The sequencing results indicated that one allele of the genome was proven to have a site-directed insertion of luciferase gene and another allele of the genome was confirmed to have additional 48 bp insertion in this cell line. The cell line was further demonstrated to be a sensitive reporter of the endogenous MMP12 promoter by applying transcription factors STAT3, AP-1 and SP-1 to the cell line. The reporter cell line was then screened with bioactive small molecule library, and a small molecule Tanshinone I was found to significantly inhibit the transcriptional activity of MMP12 gene in HEK293-MMP12-T2A-luciferase-KI cell line by luciferase activity assay, which was further confirmed to inhibit the expression of MMP12 mRNA in wild-type HEK293 cells. Conclusions: This novel luciferase knock-in reporter system will be helpful for investigating the transcriptional regulation of MMP12 gene and screening the drugs targeting MMP12 gene.


Subject(s)
Humans , Matrix Metalloproteinase 12/genetics , CRISPR-Cas Systems , Luciferases/genetics , Transcription, Genetic , Cell Communication , Cell Line , Promoter Regions, Genetic/genetics , Cell Culture Techniques , Extracellular Matrix , Gene Knock-In Techniques , Clustered Regularly Interspaced Short Palindromic Repeats
16.
Araçatuba; s.n; 2020. 57 p. ilus, graf, tab.
Thesis in English | LILACS, BBO | ID: biblio-1428496

ABSTRACT

As infecções endodônticas são causadas por uma comunidade multiespécie de bactérias. Com os métodos de identificação microbiológica e quantificação de endotoxinas, é difícil inferir a fisiologia, a função e a patogenicidade microbiana. Assim, a análise proteômica é uma técnica que pode revolucionar o estudo da patogênese das infecções endodônticas. O presente estudo tem por objetivo analisar o perfil proteômico de infecções endodônticas relacionadas a dentes com periodontite apical sintomática ou assintomática utilizando cromatografia líquida associada à espectrometria de Massas. A análise deste perfil proteômico visa a proporcionar a compreensão dos aspectos ecológicos e patogênicos do comportamento de comunidades bacterianas endodônticas através da identificação de proteínas expressas no referido ambiente no momento da coleta e a determinação da função dessas proteínas. Foram coletadas amostras de 18 pacientes encaminhados para tratamento de canal radicular ou tratamento de emergência na Faculdade de Odontologia de Araçatuba FOA ­ UNESP. Foram incluídos dentes com infecção endodôntica primária, sintomáticos ou assintomáticos. A identificação dos peptídeos foi feita num sistema nanoACQUITY UPLC-Xevo QTof MS system (Waters), a identificação das proteínas foi obtida utilizando o software Protein Lynx Global Server (PLGS) versão 3.0, utilizando o banco de dados de proteínas UniProtKB. A diferença de expressão entre os grupos foi obtida através do mesmo software, considerando-se p<0,05 para as proteínas subreguladas e 1-p>0,95 para as proteínas suprareguladas. Foram identificados um total de 2181 números de acessos entre fragmentos, isoformas e proteínas completas humana e 51 proteínas bacterianas e ambas foram classificadas quanto a sua função biológica, em relação às proteínas exclusivas de cada grupo, 347 proteínas foram identificadas no grupo sintomático. As funções biológicas mais prevalentes foram comunicação celular e transdução de sinais, seguidas pela resposta imune, observou-se diversas proteínas exclusivamente expressas no grupo sintomático, indicando a influência direta da periodontite sintomática na resposta do hospedeiro(AU)


Endodontic infections are caused by a multispecies community of bacteria. With microbiological identification methods and endotoxin quantification, it is difficult to infer physiology, function and microbial pathogenicity. Thus, proteomic analysis is a technique that can revolutionize the study of the pathogenesis of endodontic infections. The present study aims to analyze the proteomic profile of endodontic infections related to teeth with symptomatic or asymptomatic apical periodontitis using liquid chromatography associated with mass spectrometry. The analysis of this proteomic profile aims to provide an understanding of the ecological and pathogenic aspects of the behavior of endodontic bacterial communities by identifying proteins expressed in the said environment at the time of collection and determining the function of these proteins. Samples were collected from 18 patients referred for root canal treatment or emergency treatment at the School of Dentistry of Araçatuba FOA - UNESP. Teeth with primary endodontic infection, symptomatic or asymptomatic were included. The identification of peptides was made in a nanoACQUITY UPLC-Xevo QTof MS system (Waters) system, the identification of proteins was obtained using protein lynx global server (PLGS) software version 3.0, using the UniProtKB protein database. The difference in expression between the groups was obtained through the same software, considering p<0.05 for subregulated proteins and 1-p>0.95 for the superregulated proteins. A total of 2,181 access numbers were identified between human fragments, isoforms and complete proteins and 51 bacterial proteins, and both were classified as their biological function, in relation to the exclusive proteins of each group, 347 proteins were identified in the symptomatic group. The most prevalent biological functions were cellular communication and signal transduction, followed by the immune response, and several proteins were observed exclusively expressed in the symptomatic group, indicating the direct influence of symptomatic periodontitis on the host response(AU)


Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Periapical Periodontitis , Bacteria , Dental Pulp Cavity , Periodontitis , Tooth , Bacterial Proteins , Signal Transduction , Cell Communication , Proteomics , Immunity
17.
Protein & Cell ; (12): 866-880, 2020.
Article in English | WPRIM | ID: wpr-880881

ABSTRACT

For multicellular organisms, cell-cell communication is essential to numerous biological processes. Drawing upon the latest development of single-cell RNA-sequencing (scRNA-seq), high-resolution transcriptomic data have deepened our understanding of cellular phenotype heterogeneity and composition of complex tissues, which enables systematic cell-cell communication studies at a single-cell level. We first summarize a common workflow of cell-cell communication study using scRNA-seq data, which often includes data preparation, construction of communication networks, and result validation. Two common strategies taken to uncover cell-cell communications are reviewed, e.g., physically vicinal structure-based and ligand-receptor interaction-based one. To conclude, challenges and current applications of cell-cell communication studies at a single-cell resolution are discussed in details and future perspectives are proposed.


Subject(s)
Animals , Humans , Cell Communication , RNA-Seq , Single-Cell Analysis , Transcriptome
18.
International Journal of Oral Science ; (4): 17-17, 2020.
Article in English | WPRIM | ID: wpr-828961

ABSTRACT

Homoeostasis depends on the close connection and intimate molecular exchange between extracellular, intracellular and intercellular networks. Intercellular communication is largely mediated by gap junctions (GJs), a type of specialized membrane contact composed of variable number of channels that enable direct communication between cells by allowing small molecules to pass directly into the cytoplasm of neighbouring cells. Although considerable evidence indicates that gap junctions contribute to the functions of many organs, such as the bone, intestine, kidney, heart, brain and nerve, less is known about their role in oral development and disease. In this review, the current progress in understanding the background of connexins and the functions of gap junctions in oral development and diseases is discussed. The homoeostasis of tooth and periodontal tissues, normal tooth and maxillofacial development, saliva secretion and the integrity of the oral mucosa depend on the proper function of gap junctions. Knowledge of this pattern of cell-cell communication is required for a better understanding of oral diseases. With the ever-increasing understanding of connexins in oral diseases, therapeutic strategies could be developed to target these membrane channels in various oral diseases and maxillofacial dysplasia.


Subject(s)
Humans , Bone and Bones , Cell Communication , Connexins , Metabolism , Physiology , Gap Junctions , Metabolism , Pathology , Homeostasis , Physiology , Mouth Diseases , Phosphorylation
19.
Journal of Central South University(Medical Sciences) ; (12): 440-448, 2020.
Article in English | WPRIM | ID: wpr-827422

ABSTRACT

Exosomes are in a size of 30-100 nm vesicles released by various cells, with a double-layered lipid membrane containing DNA, RNA, and protein. In the past, exosomes were considered to be molecular waste, and recently exosomes have been shown to be involved in many pathophysiological processes, including intercellular communication, immune response, nerve repair, and tumorigenesis. Exosomes are present in numerous body fluids, and urinary exosomes have been shown to be biomarkers of a variety of kidney diseases.


Subject(s)
Humans , Biomarkers , Cell Communication , Exosomes , Kidney Diseases , Proteins
20.
Journal of Dental Anesthesia and Pain Medicine ; : 77-82, 2019.
Article in English | WPRIM | ID: wpr-740007

ABSTRACT

It is well known that trigeminal nerve injury causes hyperexcitability in trigeminal ganglion neurons, which become sensitized. Long after trigeminal nerve damage, trigeminal spinal subnucleus caudalis and upper cervical spinal cord (C1/C2) nociceptive neurons become hyperactive and are sensitized, resulting in persistent orofacial pain. Communication between neurons and non-neuronal cells is believed to be involved in these mechanisms. In this article, the authors highlight several lines of evidence that neuron-glial cell and neuron macrophage communication have essential roles in persistent orofacial pain mechanisms associated with trigeminal nerve injury and/or orofacial inflammation.


Subject(s)
Cell Communication , Cervical Cord , Facial Pain , Inflammation , Macrophages , Neurons , Nociceptors , Trigeminal Ganglion , Trigeminal Nerve , Trigeminal Nerve Injuries , Trigeminal Nucleus, Spinal
SELECTION OF CITATIONS
SEARCH DETAIL