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1.
Medisan ; 25(3)2021. tab, ilus
Article in Spanish | LILACS, CUMED | ID: biblio-1287303

ABSTRACT

Introducción: La sangre ovina constituye un suplemento esencial en la elaboración de medios de cultivo, dado que aporta factores nutricionales indispensables para el crecimiento y la recuperación de diversos microorganismos. Objetivo: Evaluar comparativamente el efecto de la sangre ovina, tanto citratada como desfibrinada, en medios de cultivo de base agar en cuanto al crecimiento bacteriano y la producción de hemólisis de cepas de referencia de diferentes bacterias patógenas, así como la recuperación o el aislamiento de microorganismos de muestras clínicas. Métodos: Se realizó un estudio observacional, descriptivo y transversal en 6 laboratorios de microbiología de la ciudad de Santiago de Cuba durante el año 2017, en los cuales se empleó sangre de ovinos de la raza pelibuey, para la elaboración de medios de cultivo. A cada laboratorio se le suministró tanto sangre citratada como desfibrinada y se le entregó una encuesta para valorar los resultados. Resultados: Existió un mejor crecimiento y aislamiento bacteriano en el medio suplementado con sangre desfibrinada, a pesar de que el rendimiento o los resultados en el caso de la sangre citratada resultaron satisfactorios. Conclusiones: Se confirmó la pertinencia del uso de la sangre desfibrinada como suplemento de enriquecimiento nutritivo en medios de cultivo; no obstante, quedó demostrada la utilidad de la sangre citratada en la labor de rutina del laboratorio de microbiología clínica.


Introduction: Sheep blood is an essential supplement in the elaboration of culture media, as it provides important nutritional factors for the growth and recovery of different organisms. Objective: To evaluate comparatively the effect of sheep citrated and defibrinated sheep blood in culture media with agar base as for the bacterial growth and the production of hemolysis in reference strains from different pathogen bacteria, as well as the recovery or isolation of microorganisms from clinical samples. Method: An observational, descriptive and cross-sectional was carried out in 6 microbiology laboratories in Santiago de Cuba city during 2017, in which male sheeps blood from the pelibuey breed for elaborating culture media. Each laboratory received either citrated blood or defibrinated and a survey was delivered to evaluate the results. Results: There was a better growing and bacterial isolation in the media supplemented with defibrinated blood, although yielding or results were favorable with citrated blood. Conclusions: The pertinence of the use of defibrinated blood as a supplement of nutritive enrichment in culture media was confirmed; however, the use of citrated blood was demonstrated in the routine work of the clinical microbiology laboratory.


Subject(s)
Sheep , Bacterial Growth , Culture Media , Hemolysis
2.
Rev. Fundac. Juan Jose Carraro ; 24(44): 40-47, 2021. ilus, tab
Article in Spanish | LILACS | ID: biblio-1223492

ABSTRACT

Las enfermedades del periodonto tienen una etiopatogenia compleja y puede considerarse multifactorial. El factor etiológico esencial en la patología inflamatoria periodontal es la biopelícula dental y cuando el desequilibrio entre el huésped y los microorganismos cambia la complejidad de la flora. Ciertas bacterias como Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Prevotella loescheii, Fusobacterium nucleatum, Tannerrella forsythia, Campylobacter rectus, Eikenella corrodens y Treponema spp., han sido comúnmente relacionadas con la periodontitis crónica y son consideradas como indicadores de riesgo para la progresión de dicha enfermedad. El objetivo de este trabajo fue establecer la prevalencia de Prevotella spp y Porphyromona spp en los distintos estadios de periodontitis crónicas. Material y métodos: Se estudiaron 48 pacientes sistémicamente saludables con diagnóstico de periodontitis crónica. Se completó el consentimiento informado, se realizó historia clínica y examen periodontal. El estado periodontal se clasificó en distintos grados de severidad: leve, moderada y severa. Se tomaron muestras de dos sitios con mayor profundidad de sondaje con conos de papel absorbente estériles y se transportaron en un medio prerreducido. Para el aislamiento de Prevotella spp se utilizó agar Brucella más sangre ovina al 5%, hemina, vitamina K al que se agregaron vancomicina y kanamicina; Porphyromonas sp se aisló en el mismo medio con el agregado de bacitracina y colistina. Se sembraron 10 µl de muestra entera y las placas fueron incubadas en jarras de anaerobiosis por 5 a 7 días a 37ºC. Resultados: los distintos grados de periodontitis correspondieron a un 17% periodontits leve, 57% moderada y 26% severa. En el total de pacientes se determinó la presencia de Prevotella spp en el 54% de los casos y un 12,5% de Porphyromona spp. Conclusión: De los pacientes estudiados con periodontits crónica, un 52% correspondió al sexo masculino, un 57% de los casos correspondieron a periodontitis moderada. Se aisló Prevotella sp en todos los estadios de periodontitis crónica y Porphyromonas sp sólo en periodontitis severas (AU)


Periodontal diseases have a complex etiopathogenesis and can be considered multifactorial. The essential etiological factor in periodontal inflammatory pathology is the dental biofilm and when the imbalance between the host and the microorganisms changes the complexity of the flora. Certain bacteria such as Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Prevotella loescheii, Fusobacterium nucleatum, Tannerrella forsythia, Campylobacter rectus, Eikenella corrodens and Treponema spp., Have been commonly related to chronic periodontitis and are considered as risk indicators for the progression of said disease. The objective of this work was to establish the prevalence of Prevotella spp and Porphyromonas spp in the different stages of chronic periodontitis. Forty eight systemically healthy patients diagnosed with chronic periodontitis were studied. Informed consent was completed, a medical history and periodontal examination was carried out. The periodontal state was classified into different degrees of severity: mild, moderate and severe. Samples were taken from two sites with greater depth of probing with sterile absorbent paper cones and transported in a prereduced medium. For the isolation of Prevotella spp, Brucella agar plus 5% sheep blood, hemin, vitamin K to which vancomycin and kanamycin were added. For Porphyromonas spp, the same medium was used and bacitracin and colistin were added. 10 µl of the whole sample was seeded and the plates were incubated in anaerobic jars for 5 to 7 days at 37 ° C. Different degrees of periodontitis corresponded to 17% mild periodontitis, 57% moderate and 26% severe. In the total number of patients, the presence of Prevotella spp was determined in 54% of the cases and 12.5% of Porphyromona spp. Of the patients studied with chronic periodontitis, 52% corresponded to the male sex, 57% of the cases corresponded to moderate periodontitis. Prevotella spp was isolated in all stages of chronic periodontitis and Porphyromonas sp only in severe periodontitis (AU)


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Porphyromonas gingivalis/isolation & purification , Prevotella intermedia/isolation & purification , Chronic Periodontitis/microbiology , Colony Count, Microbial , Risk Factors , Culture Media , Dental Plaque/microbiology , Age and Sex Distribution
3.
Braz. j. med. biol. res ; 54(11): e11191, 2021. tab, graf
Article in English | LILACS | ID: biblio-1285664

ABSTRACT

The present study focused on the scenario of confirmed cases of SARS-CoV-2 infection in the state of Minas Gerais (MG), Brazil, from March 2020 to March 2021. We evaluated the evolution of COVID-19 prevalence and death in one municipality from each of the 14 health macro-regions of MG state. Socio-demographic characteristics and variables related to the municipalities were analyzed. The raw dataset used in this study was freely sourced from the website Brasil.io. From the raw dataset, two time series were extracted: the cumulative confirmed cases of COVID-19 and cumulative death counts, and they were compared to the state data using a nowcasting approach. In order to make time series comparisons possible, all data was normalized per 100,000 inhabitants. When analyzing in light of colored wave code interventions initiated in August 2020 in MG, for the majority of the municipalities, there was an absence of clear influence on prevalence and deaths. The national holidays in the first semester of 2020 had a small impact on the COVID-19 prevalence of the municipalities, but the holidays in the second semester of 2020 and beginning of 2021 caused important impacts on COVID-19 prevalence. The low number of ICU beds in some municipalities contributed to the higher number of deaths. The analysis showed here is expected to contribute to the improvement of decision making of the MG government, as it opened a huge possibility to have the total macro-regions and state data analyzed.


Subject(s)
Humans , COVID-19 , Brazil/epidemiology , Cities/epidemiology , Culture Media , SARS-CoV-2
4.
Vaccimonitor (La Habana, Print) ; 29(2)mayo.-ago. 2020. graf
Article in Spanish | LILACS, CUMED | ID: biblio-1127511

ABSTRACT

Streptococcus pneumoniae es un patógeno oportunista que puede causar infecciones como otitis media, neumonía, sepsis y meningitis. Sin embargo, existen muchas limitaciones para el cultivo en zaranda de este microorganismo en los laboratorios de microbiología. Por esta razón, se realizó un estudio de la consistencia del cultivo en zaranda de Streptoccoccus pneumoniae 19A a escala de 40 L. Para esto se desarrolló previamente la curva de crecimiento patrón hasta las 5 h. Desde el pre-inóculo se inocularon 6 frascos de 100 mL, de los cuales se realizó la inoculación en botellones de 1 L y 5 L. En todos los casos, se determinó la pureza a partir de la tinción de Gram y el crecimiento bacteriano se monitoreó por el método de conteo de viables y la densidad óptica cada 1 h. Además, se evaluó el rendimiento del cultivo a partir de la cantidad de biomasa obtenida por peso húmedo. Cada proceso se llevó a cabo en condiciones iguales por triplicado. En los tres procesos se obtuvieron curvas de crecimiento similares, tanto por densidad óptica como por conteo de viables, alcanzando una viabilidad máxima de 109 UFC/mL en la última escala. Además, se obtuvieron rendimientos de biomasa de 11,62; 11,92 y 11,60 g/L, respectivamente. Estos resultados demuestran que la metodología utilizada ofrece una consistencia de este proceso, a pesar del alto volumen de cultivo en zaranda, lo cual no afectó la calidad de la biomasa, demostrado por la viabilidad final(AU)


Streptococcus pneumoniae is an opportunistic pathogen that can cause infections including otitis media, pneumonia, sepsis and meningitis. However, there are many limitations for the cultivation in shaker of this microorganism in microbiology laboratories. For this reason, a study of the consistency of the culture in shaker of Streptoccoccus pneumoniae 19A was carried out at a scale of 40 L. The pattern growth curve was made until 5 h, under our laboratory conditions and culture medium. From the pre-inoculum 6 bottles of 100 mL were inoculated, from which the scaling was accomplished to bottles of 1 L and 5 L. In all cases the purity was determined by Gram staining and the bacterial growth by viable counting method and the optical density were monitored every 1 h. In addition, the yield was evaluated from the determination of the amount of biomass obtained by wet weight. Each process has been made in equal conditions in triplicate. In the three processes, similar growth curves were obtained both by optical density and by viable counts, reaching a maximum viability of 109 CFU/mL on the last scale. In addition, biomass yields of 11.62, 11.92 and 11.60 g/L were obtained, respectively. These results demonstrate that the methodology used offers a high process consistency, despite the high volume of culture in rotational shaker, and did not affect the quality of the biomass, which could be demonstrated by the viable count(AU)


Subject(s)
Pneumococcal Infections , Biomass , Culture Media
5.
Rev. Asoc. Odontol. Argent ; 108(2): 46-51, mayo-ago. 2020. tab
Article in Spanish | LILACS | ID: biblio-1121108

ABSTRACT

Objetivos: Comparar ex vivo la eficacia del instrumento XP-endo Finisher y del sistema EndoActivator en la reducción/eliminación del biofilm microbiano en conductos radiculares infectados. Materiales y métodos: Se utilizaron 23 premolares inferiores humanos extraídos cuya longitud fue estandarizada en 17 mm. Todos los conductos se prepararon con el sistema WaveOne Gold Medium (#35.06). Los dientes se esterilizaron, se inocularon con Enterococcus faecalis y se separaron en dos grupos experimentales de 10 piezas cada uno. De los 3 dientes remanentes, 1 fue utilizado como control positivo y 2, como controles negativos. En el grupo 1, las soluciones irrigantes se agitaron con XP-endo Finisher. En el grupo 2, se utilizó EndoActivator. Se tomaron muestras antes de la contaminación, luego de esta y después de la agitación de los irrigantes mediante conos de papel estériles. La carga microbiana fue sembrada en agar sangre y los conos se cultivaron en caldo tripteína de soja. La remoción de la carga microbiana se determinó por la presencia o ausencia de turbiedad del medio. Las unidades formadoras de colonias (UFC) remanentes se cuantificaron y los resultados se categorizaron como R1 (≤10 UFC) o R2 (>10 UFC). Los datos fueron analizados mediante la prueba de Fisher. Resultados: No hubo diferencias significativas entre XP-endo Finisher y EndoActivator (P>0,05). El número de usos no influyó sobre la capacidad operativa de ambos instrumentos (AU)


Aim: To compare ex vivo the effectiveness of the XP-endo Finisher and the EndoActivator in biofilm reduction/ removal from infected root canals. Materials and methods: Twenty three extracted human single-rooted lower premolars were selected and standardised to 17 mm in length. All the canals were prepared with WaveOne Gold Medium reciprocating files (#35.06). The teeth were autoclaved and inoculated with Enterococcus faecalis. The infected teeth were then assigned to 2 experimental groups of 10 teeth each according to the final irrigation/agitation protocol. Of the three remaining teeth, one was used as a positive control, and the other two were used as negative controls. In Group 1 the irrigating solutions were agitated with XP-endo Finisher while in Group 2 the EndoActivator was used. All root canals were sampled before and after contamination, and again after irrigant agitation with sterile paper points. The microbial load was spread on blood agar plates and the paper points were cultured in sterile trypticase soy broth. The removal of the microbial load was determined by visual observation of the turbidity of the media and by quantification of the number of colony-forming units (UFC). The results were categorized as R1 (≤10 UFC) or R2 (>10 UFC). Data were analysed by the Fisher's exact test at P<0.05. Results: No significant differences was found between XP-endo Finisher and EndoActivator (P>0.05) regarding their effectiveness in the reduction/removal of the microbial biofilm. The number of uses of both instruments did not affect their operative performance (AU) Conclusion: XPF and EA were both equally effective for microbial biofilm reduction/removal from ex vivo infected root canals (AU)


Subject(s)
Root Canal Irrigants/chemistry , Dental High-Speed Equipment , Biofilms , Dental Instruments , Dental Pulp Cavity/microbiology , In Vitro Techniques , Colony Count, Microbial/methods , Efficacy , Statistical Analysis , Enterococcus faecalis/isolation & purification , Culture Media
6.
Medisan ; 24(2)mar.-abr. 2020. tab
Article in Spanish | LILACS-Express | LILACS, CUMED | ID: biblio-1098393

ABSTRACT

Introducción: Las enfermedades transmitidas por alimentos se producen por ingestión de un alimento, incluido el agua, que puede estar contaminado por diversos agentes. Objetivo: Caracterizar los agentes bacterianos aislados en brotes de enfermedades transmitidas por alimentos. Métodos: Se realizó un estudio observacional, descriptivo y transversal de 100,0 % de los brotes de enfermedades transmitidas por alimentos en la provincia de Santiago de Cuba, desde enero de 2018 hasta diciembre de 2019, para lo cual se seleccionaron muestras de alimentos y heces fecales. La caracterización de las bacterias aisladas se basó en los resultados del crecimiento y otras pruebas bioquímicas-metabólicas. Se utilizaron resultados del aislamiento y confirmación de los agentes identificados en cada uno de los brotes a partir de las muestras antes citadas. Entre las variables analizadas figuraron: número de brotes, muestras de alimentos, de heces fecales y resultados de pruebas bioquímicas y metabólicas. Resultados: Se obtuvo un aislamiento de agentes bacterianos en 100,0 % de las muestras de alimentos. Hubo una mayor frecuencia de bacterias Gram negativas (82,0 %) y la menor correspondió a microorganismos Gram positivos (18,0 %). La Salmonella D fue el microorganismo más frecuente. Conclusiones: Este resultado representa un instrumento para el diagnóstico etiológico de los brotes de enfermedades transmitidas por alimentos en Santiago de Cuba.


Introduction: Diseases transmitted by foods are produced due to ingestion of a food, including water that can be contaminated by diverse agents. Objective: To characterize the bacterial agents isolated in diseases outbreaks transmitted by foods. Methods: An observational, descriptive and cross-sectional study of 100.0 % of the diseases outbreaks transmitted by foods in Santiago de Cuba, from January, 2018 to December, 2019 was carried out, for which samples of foods and stools were selected. The characterization of the isolated bacterias was based on the results of growth and other biochemical-metabolic tests. Results of the isolation and confirmation of agents identified in each one of the outbreaks from the samples mentioned above were used. Among the analyzed variables we can mention: number of outbreaks, samples of foods, samples of stools and results of biochemical and metabolic tests. Results: An isolation of bacterial agents was obtained in 100.0 % of foods samples. There was a higher frequency of Gram negative bacterias (82.0 %) and the lower corresponded to Gram positive microorganisms (18.0 %). Salmonella D was the most frequent microorganism. Conclusions: This result represents an instrument for the etiological diagnosis of diseases outbreaks transmitted by foods in Santiago de Cuba.


Subject(s)
Culture Media , Foodborne Diseases/epidemiology
7.
Rev. bras. ortop ; 55(2): 156-162, Mar.-Apr. 2020. tab, graf
Article in English | LILACS | ID: biblio-1138000

ABSTRACT

Abstract Objective To compare antisepsis techniques using chlorhexidine-based soap associated with ethyl alcohol and alcohol-based chlorhexidine or chlorhexidine-based soap associated with alcohol-based chlorhexidine alone in surgical orthopedic procedures. Methods This is a primary, randomized, analytical and single-center clinical trial consisting of 170 patients, who were divided into 2 groups. The combinations chlorhexidine-based soap + alcohol-based chlorhexidine (CSAC) and chlorhexidine-based soap + 70% ethyl alcohol + alcohol-based chlorhexidine (CSAAC) were tested in each group. The cultures were grown in mannitol and eosin methylene blue (EMB) after collection before skin preparation (time point 0), after skin preparation (time point 1) and at the end of the surgical procedure (time point 2). Results There was no statistically significant difference regarding bacterial growth in mannitol and EMB between the groups at any time point. Moreover, there was no statistical difference between groups and time points regarding the type of bacterial growth in culture media. Conclusion There was no difference between these antisepsis techniques for the prevention of surgical site infection in orthopedic procedures; in addition, a protocol containing measures to prevent infection in such procedures was developed.


Resumo Objetivo Comparar as técnicas de antissepsia utilizando clorexidina degermante associada a álcool etílico e a clorexidina alcoólica versus clorexidina degermante associada a clorexidina alcoólica, em procedimentos cirúrgicos ortopédicos. Métodos Trata-se de um estudo clínico, primário, randomizado, analítico e de centro único, constituído por 170 pacientes ortopédicos submetidos a abordagem cirúrgica, alocados em 2 grupos aleatórios, nos quais foram testados clorexidina degermante + clorexidina alcoólica (grupo CDCA) e clorexidina degermante + álcool etílico a 70% + clorexidina alcoólica (grupo CDACA). Foram realizadas culturas nos meios manitol e eosina azul de metileno (EAM) de amostras colhidas nos períodos de pré-degermação (0), pós-degermação (1) e após a incisão suturada (2). Resultados Em relação ao crescimento bacteriano nos meios de cultura manitol e EAM entre os grupos, em cada período de estudo (0, 1 e 2), não ocorreu diferença estatística significativa nesta pesquisa. Na avaliação do tipo de crescimento bacteriano nos meios de cultura manitol e EAM, também não foi constatada significância estatística entre os grupos. Conclusão Não ocorreu diferença entre as técnicas utilizadas na antissepsia para prevenção de infecção de sítio cirúrgico em procedimentos ortopédicos, mas, ao final do trabalho, foi possível a elaboração de um protocolo de medidas para realização de prevenção infecciosa nesses procedimentos.


Subject(s)
Humans , Operating Rooms , Orthopedics , Surgical Procedures, Operative , Bacterial Growth , Chlorhexidine , Antisepsis , Orthopedic Procedures , Culture Media , Disease Prevention , Infections , Mannitol
8.
Acta amaz ; 50(1): 61-67, jan. - mar. 2020.
Article in English | LILACS | ID: biblio-1118663

ABSTRACT

The high diversity of the genus Geastrum and the difficulty of obtaining mycelial cultures impairs the study of the ecophysiology and the exploration of the biotechnological potential of the taxon. In this study, different culture media were tested to obtain mycelial cultures for G. lloydianum and G. subiculosum collected in the Brazilian Amazon. Data on spore germination, and isolation of monokaryotic cultures and in vitro sexual reproduction are presented, as well as a brief morphological description of the cultures obtained. For both species, Potato Dextrose Agar (PDA) was the most promising of the tested culture media. The highest growth in agar culture ever recorded for this genus is reported (4.9 mm per week for G. lloydianum and 7.5 mm for G. subiculosum). In the PDA culture medium, spores germinated after 35-40 days of incubation and the isolation of monokaryotic cultures of the two species, as well as in vitro sexual crosses, were successfully performed. (AU)


Subject(s)
Spores , Amazonian Ecosystem , Culture Media , Fungi
9.
J. oral res. (Impresa) ; 9(1): 29-35, feb. 28, 2020. ilus, tab
Article in English | LILACS | ID: biblio-1151424

ABSTRACT

The present in vitro study compared the inhibitory action of five pediatric toothpastes against Streptococcus mutans ATCC 25175. Materials and Methods: Cross-sectional, comparative and experimental study. The microorganism Streptococcus mutans ATCC 25175 was inoculated onto solid culture medium of Müeller-Hinton supplemented with blood, then the plates were inoculated with five pediatric toothpastes: Oral B Stages, Colgate Smiles, Aquafresh My Little Teeth, Dentito and Denture Kids. Samples were incubated at 37°C for 48 hours. Subsequently the inhibition halos were measured; the experiment was repeated 20 times for each sample. Statistical analysis was performed with ANOVA complemented with Tukey's test. Results: Oral B Stages yielded a mean inhibitory halo of 23.2mm, Colgate Smiles an average of 21.7mm, Aquafresh My Little Teeth of 13.6mm, Dentito of 18.5mm, and Denture Kids a mean of 23.0mm. When performing the ANOVA test, it was found that there was a significant difference in the inhibitory action of pediatric toothpastes (p<0.005) and when using Tukey's multiple comparison test, Oral B and Denture Kids presented similar inhibitory action. Conclusion: All toothpastes presented inhibitory action against Streptococcus mutans ATCC 25175. A significant difference between their effectiveness was observed. Oral B Stages showed the greatest degree of inhibition.


El presente estudio in vitrocomparo la eficacia inhibitoria de cinco pastas dentales pediátricas frente a la bacteria Streptococcus mutansATCC25175. Material y Métodos: El estudio fue transversal, comparativo y experimental. Se inoculó Streptococcus mutansATCC 25175 en un medio de cultivo Müeller-Hinton complementado con sangre, luego a las placas cultivadas se le inocularon cincopastas dentales pediátricas: Oral B Stages, Colgate Smiles, Aquafresh My LittleTeeth, Dentito y Denture Kids. Se incubó a 37ºC por 48 horas y posteriormente semidió los halos de inhibición, se replicó el experimento 20 veces para cada uno. El análisis estadístico se realizó con el test de ANVA complementado con el test de Tukey. Resultado: Oral B Stages generó una media inhibitoria de 23,2mm, ColgateSmiles una media de 21,7mm, Aquafresh My Little Teeh de 13,6mm, Dentitode 18,5mm y finalmente Denture Kids una media de 23,0mm. Al realizar laprueba ANOVA se encontró que hay diferencia significativa en la acción inhibitoriade las pastas dentales pediátricas (p<0.005) y al emplear la prueba Tukey (comparación de múltiples) la pasta Oral B y Denture Kids presentaron acción inhibidora similar. Conclusión:Todas las pastas presentaron acción inhibitoria sobre Streptococcus mutansATCC 25175 existiendo diferencia significativa entre la efectividad de estas, con la pasta Oral B Stages demuestrando mayor acción inhibitoria.


Subject(s)
Humans , Child , Streptococcus mutans , Toothpastes/analysis , Oral Hygiene , Peru , In Vitro Techniques , Cross-Sectional Studies , Culture Media , Dental Caries
10.
J. oral res. (Impresa) ; 9(1): 57-62, feb. 28, 2020. graf, tab
Article in English | LILACS | ID: biblio-1151489

ABSTRACT

The ageing of population is increasing, and a great percentage of these patients wear removable prostheses, and can suffer denture stomatitis, a condition that has been associated with candidiasis. Aims: To evaluate in vitro the effectiveness of Copper Sulfate against Candida albicans in samples of heat-polymerized acrylic resin, compared to nystatin, sodium hypochlorite and chlorhexidine. Materials and Methods: Initially, the minimum inhibitory concentration (MIC) of copper sulfate for Candida albicans was determined by microdilution. Then, 54 resin samples were divided into 6 treatment groups corresponding to Nystatin 100.000 UI, Sodium Hypochlorite 0.5%, chlorhexidine 0.12%, Copper Sulfate 4.7µg/ml, Copper Sulfate 9.4µg/ml and physiological saline solution, in which samples were submerged for 6 hours. Resin samples were then washed and cultured on solid media at 37°C for 72 hours. The number of colony-forming units was determined using a Quebec colony counter. The statistical analysis was performed using the Kruskal-Wallis test and the Mann-Whitney U test. Results: Copper sulfate at a concentration of 9.4µg/ml presented a similar effectiveness as the other control products regarding the reduction in the number of colonies of Candida albicans post-treatment. Conclusion: The effectiveness of copper sulfate against Candida albicans on acrylic resin was similar to that of nystatin, sodium hypochlorite and chlorhexidine.


En las últimas décadas se ha observado un aumento de la población de adultos mayores, de los cuales un gran porcentaje es portador de prótesis removible, y dos tercios pueden sufrir estomatitis subprotésica, enfermedad que es asociada a infecciones como candidiasis. Objetivo: Evaluar la efectividad antimicótica in vitro del sulfato de cobre en placas de resinas acrílicas de termocurado inoculadas con Candida albicans, frente a Nistatina, Hipoclorito de Sodio y Clorhexidina. Material y Métodos: Inicialmente, y mediante microdilución del sulfato de cobre, se determinó la concentración mínima inhibitoria (CMI) para Candida albicans. En la fase experimental, 54 muestras de resina se dividieron en 6 grupos correspondientes a Nistatina 100.000 UI, Hipoclorito 0.5%, Clorhexidina 0.12%, Sulfato de Cu 4.7µg/ml, Sufato de Cu 9.4 µg/ml y suero fisiológico. Las muestras fueron sumergidas en estos agentes por 6 horas, para posteriormente ser lavadas y cultivada en medios solidos a 37°C por 72 horas. Luego se realizó el conteo de unidades formadoras de colonias mediante contador tipo Quebec. El análisis estadístico se realizó mediante la prueba de Kruskal-Wallis y la prueba U de Mann-Whitney. Resultado: El sulfato de cobre a una concentración de 9.4µg/ ml presentó una efectividad similar a los otros productos, en la reducción de colonias de Candida albicans. Conclusión: La efectividad del sulfato de cobre contra Candida albicans fue semejante a la de Nistatina, Hipoclorito y Clorhexidina.


Subject(s)
Humans , Acrylic Resins , Candida albicans/drug effects , Copper Sulfate/pharmacology , Sodium Hypochlorite , Stomatitis, Denture , In Vitro Techniques , Candidiasis/drug therapy , Chlorhexidine , Culture Media
11.
Article in English | WPRIM | ID: wpr-816615

ABSTRACT

BACKGROUND: Acromegaly is a rare disease primarily caused by growth hormone (GH)-secreting pituitary adenomas, and its treatment is costly. Moreover, some patients are unresponsive to treatment. Hence, there are increasing efforts to develop new drugs with improved effectiveness for this disease. BIM23B065 is a novel chimeric molecule that acts on both somatostatin and dopamine receptors. This study aimed to investigate the effects of BIM23B065 compared with those of a somatostatin receptor analog and a dopamine agonist.METHODS: The effects of BIM23B065 on the proliferation, GH and insulin-like growth factor-1 (IGF-1) levels, and extracellular signal-regulated kinase (ERK) 1/2 and cyclic AMP response element binding (CREB) phosphorylation of GH3 cells were investigated with MTS assay, enzyme-linked immunosorbent assay, and Western blotting, respectively. The dosage and treatment duration of BIM23B065 were tested in animal models of GH-secreting pituitary adenoma. The effect of BIM23B065 (3 mg/kg/day) on changes in IGF-1 levels before and after treatment was further investigated.RESULTS: In vitro, BIM23B065 treatment decreased GH release in the culture media and downregulated ERK 1/2 and CREB phosphorylation to 22% and 26%, respectively. In vivo, IGF-1 expression decreased to 50 % after 4 weeks of treatment with BIM23B065 using an osmotic pump implant. Moreover, magnetic resonance imaging results showed that the tumor size decreased significantly following treatment with BIM23B065 for 4 weeks.CONCLUSION: The novel chimeric molecule was effective in decreasing IGF-1 and GH levels and may serve as an effective therapeutic agent for acromegaly.


Subject(s)
Acromegaly , Blotting, Western , Culture Media , Cyclic AMP , Dopamine Agonists , Dopamine , Enzyme-Linked Immunosorbent Assay , Growth Hormone , Growth Hormone-Secreting Pituitary Adenoma , Humans , In Vitro Techniques , Insulin-Like Growth Factor I , Magnetic Resonance Imaging , Models, Animal , Phosphorylation , Phosphotransferases , Pituitary Neoplasms , Rare Diseases , Receptors, Dopamine , Receptors, Somatostatin , Response Elements , Somatostatin
12.
Article in Chinese | WPRIM | ID: wpr-828507

ABSTRACT

OBJECTIVE@#To investigate the characteristics of growth and metabolism and the toxicity of under different conditions.@*METHODS@#We observed the growth of and under routine culture conditions and in different pH and salt concentrations, and compared their activities of sugar fermentation using microbiochemical reaction tubes. Four-week-old nude mice were randomized into infection group (=5), infection group (=5) and control group (=5) for intragastric administration of 0.3 mL suspension the two (5×10 cfu/mL) or 0.3 mL normal saline. Samples of the liver, kidney, intestine, feces and blood were taken for analysis of the distribution and toxicity of by fungal culture and histopathological examination.@*RESULTS@# exhibited logarithmic growth at 8-24 h after inoculation and showed stable growth after 24 h. showed optimal growth within the pH value range of 5-7 with a growth pattern identical to that of . grew better than in media containing 5% and 10% NaCl, and could ferment glucose, sucrose, trehalose and sorbitol. could be isolated from the feces, blood, liver and kidney of infected nude mice, and the liver had the highest fungal load (5.7 log cfu/g). could cause pathological changes in the liver and intestine of the mice, but with a lesser severity as compared with .@*CONCLUSIONS@# exhibits optimal growth in mildly acidic or neutral conditions with a high salt tolerance, and can potentially penetrate the intestinal barrier into blood and lead to tissue injuries in hosts with immunosuppression.


Subject(s)
Animals , Candida , Candida albicans , Candidiasis , Microbiology , Culture Media , Mice , Mice, Nude , Random Allocation
13.
Chinese Journal of Biotechnology ; (12): 2377-2386, 2020.
Article in Chinese | WPRIM | ID: wpr-878494

ABSTRACT

Surfactin has great potential applications in enhancing oil recovery, agriculture, pharmaceuticals, foods and beverages, and cosmetics due to its extraordinary surface activity, biodegradability, anti-bacterial activity and biocompatibility. Enhancing surfactin production by engineering surfactin-producer and optimizing culture conditions is the key of its industrial production and subsequent applications. In this study, the effect of fatty acid synthesis pathway on surfactin synthesis was investigated, and Bacillus subtilis THBS-2 and THBS-8 with high surfactin titer were constructed by overexpressing key genes involved in the fatty acid synthesis pathway. To optimize culture condition, the amount and adding time of isopropyl-beta-D-thiogalactopyranoside (IPTG) and amino acids were studied, and a two-stage culture method was obtained: IPTG (final concentration: 1.25 mmol/L) and leucine (final concentration: 5 g/L) were added at 3 h, leucine (final concentration 5 g/L) and condensed culture medium (5 mL) were added at 24 h. Applying this strategy, the surfactin titer of B. subtilis THBS-2 reached to 24 g/L in shake flask at 48 h and up to 34 g/L after 68 h fermentation in a 30-L fermentor. The results provide basis for large-scale production and broad application of surfactin.


Subject(s)
Amino Acids , Bacillus subtilis/metabolism , Culture Media , Fermentation , Lipopeptides , Peptides, Cyclic
14.
São Paulo; SES/SP; 2020. 39 p.
Monography in Portuguese | LILACS, SES-SP, SESSP-ESPECIALIZACAOSESPROD, SES-SP | ID: biblio-1128731

Subject(s)
Culture Media , Leptospira
15.
Clin. biomed. res ; 40(4): 213-217, 2020. tab
Article in Portuguese | LILACS | ID: biblio-1252521

ABSTRACT

Introdução: O método clássico para o diagnóstico de micoses é realizado pelo Exame Micológico Direto (EMD) e cultural, que possibilita a visualização de estruturas fúngicas vegetativas e estruturas reprodutivas, respectivamente. Essa combinação é fundamental para reduzir possíveis erros analíticos e aumentar a precisão do diagnóstico. Métodos: Com a finalidade de verificar a frequência do EMD e cultural, e comparar seus parâmetros de sensibilidade e especificidade, realizamos uma análise retrospectiva entre janeiro de 2018 e maio de 2020, de 1603 laudos micológicos oriundos de um laboratório de análises clínicas, localizado em Porto Alegre. Resultados: Após a análise dos laudos observamos que a maioria dos casos apresentaram o EMD negativo com cultura positiva (36,24%). Na sequência, 30,87% dos casos foram de amostras negativas e 25,57% dos laudos foram positivos para ambos os exames. A minoria dos casos (7,29%) apresentou o EMD positivo com cultura negativa. Conclusão: Esta análise revelou que o exame cultural é mais sensível e específico, demonstrando uma maior confiabilidade no diagnóstico. Entretanto, vale ressaltar que a realização dos exames em conjunto, além de reduzir possíveis erros analíticos, proporciona um diagnóstico melhor fundamentado. (AU)


Introduction: The classic method for the diagnosis of mycoses is performed by both direct mycological examination (DME) and culture, which allow the visualization of vegetative and reproductive fungal structures, respectively. This combination is essential to reduce possible analytical errors and increase the accuracy of the diagnosis. Methods: To assess the frequency of DME and culture, and compare their parameters of sensitivity and specificity, we performed a retrospective analysis of 1603 mycological reports produced between January 2018 and May 2020 in a clinical analysis laboratory in Porto Alegre, southern Brazil. Results: After analyzing the reports, we observed that most cases presented a negative DME and a positive culture (36.24%). Subsequently, 30.87% of the cases were negative for both tests, and 25.57% were positive for both tests. The minority of cases (7.29%) presented a positive DME and a negative culture. Conclusion: Our analysis revealed that cultural examination is more sensitive and specific, showing greater reliability in the diagnosis. However, it is noteworthy that performing the tests together, in addition to reducing possible analytical errors, provides a more consistent diagnosis. (AU)


Subject(s)
Comparative Study , Culture Media , Laboratory Test , Mycoses/diagnosis , Sensitivity and Specificity , Mycological Typing Techniques
16.
Braz. arch. biol. technol ; 63: e20180534, 2020. tab
Article in English | LILACS | ID: biblio-1132214

ABSTRACT

Abstract Microalgae are photosynthetic microorganisms whose composition and biomass production can be influenced by manipulating the cultivation conditions employed. However, few studies have evaluated the effects of various cultivation conditions in autotrophic and mixotrophic conditions. The present work aimed to evaluate the effects of cultivation conditions on the cell growth and biosynthesis of fatty acids (FAs) by microalgae of the genus Chlorella in autotrophic and mixotrophic cultivation. Evaluation of the effects of the conditions was performed using an experimental design methodology. The highest values of maximum biomass concentration (Xmax) and maximum biomass productivity (Pmax) were obtained in autotrophic cultures. Palmitic acid was the FA obtained at the highest concentration in both cultivation modes. The concentrations of polyunsaturated FAs (PUFAs) ranged from 12.2 to 41.2% in autotrophic cultures and from 11 to 34.3% in the mixotrophic cultures. The variables photoperiod and sodium bicarbonate concentration showed the greatest influence on the Xmax, Pmax, and PUFA concentration in autotrophic and mixotrophic cultivations, respectively. This study verified that the selection of conditions and mode of cultivation contribute to the production of microalgal biomass and FA biosynthesis.


Subject(s)
Chlorella/metabolism , Fatty Acids/biosynthesis , Chlorella/growth & development , Culture Media
17.
Electron. j. biotechnol ; 37: 18-24, Jan. 2019. tab, ilus, graf
Article in English | LILACS | ID: biblio-1049076

ABSTRACT

BACKGROUND: The 11S globulin from amaranth is the most abundant storage protein in mature seeds and is well recognized for its nutritional value. We used this globulin to engineer a new protein by adding a four valinetyrosine antihypertensive peptide at its C-terminal end to improve its functionality. The new protein was named AMR5 and expressed in the Escherichia coli BL21-CodonPlus(DE3)-RIL strain using a custom medium (F8PW) designed for this work. RESULTS: The alternative medium allowed for the production of 652 mg/L expressed protein at the flask level, mostly in an insoluble form, and this protein was subjected to in vitro refolding. The spectrometric analysis suggests that the protein adopts a ß/α structure with a small increment of α-helix conformation relative to the native amaranth 11S globulin. Thermal and urea denaturation experiments determined apparent Tm and C1/2 values of 50.4°C and 3.04 M, respectively, thus indicating that the antihypertensive peptide insertion destabilized the modified protein relative to the native one. AMR5 hydrolyzed by trypsin and chymotrypsin showed 14- and 1.3-fold stronger inhibitory activity against angiotensin I-converting enzyme (IC50 of 0.034 mg/mL) than the unmodified protein and the previously reported amaranth acidic subunit modified with antihypertensive peptides, respectively. CONCLUSION: The inserted peptide decreases the structural stability of amaranth 11S globulin and improves its antihypertensive activity.


Subject(s)
Peptides/metabolism , Proteins/metabolism , Globulins/metabolism , Antihypertensive Agents/metabolism , Seeds , Temperature , Culture Media , Amaranthus , Protein Stability , Phytochemicals
18.
Rev. ADM ; 76(5): 261-266, sept.-oct. 2019. ilus, tab
Article in Spanish | LILACS | ID: biblio-1052989

ABSTRACT

Los profesionales de la salud están expuestos a una gran variedad de microorganismos desde esporas, bacterias, hongos, virus y protozoarios que pueden encontrarse en la sangre y/o saliva de los pacientes. Cualquiera de estos microorganismos puede causar una enfermedad infectocontagiosa a través de pinchazos y/o salpicaduras producidas por el aerosol durante la práctica dental. Objetivo: Determinar la presencia bacteriana en las piezas de alta velocidad utilizadas en la práctica clínica. Material y métodos: Es un estudio experimental, observacional y transversal en el que se evaluó la contaminación de 30 piezas de alta velocidad utilizadas en la práctica clínica. Previo al estudio se efectuó una estandarización obteniendo una kappa del 0.85. Se realizó una base de datos en el programa SPSS versión 22, con el que se llevó a cabo el análisis descriptivo para determinar medidas de tendencia central. Resultados: 73.3% de las muestras analizadas tuvieron crecimiento bacteriano, entre las bacterias que se encontraron resultó que 54.5% de ellas fueron bacterias Gram positivas y el resto Gram negativas. La bacteria con mayor presencia en la muestra fue el Bacillus en 45.5% seguida del Streptococcus en 27.3%, el restante 27.2% fue Staphylococcus, Coccus y Streptobacillus. Conclusiones: El uso correcto de las piezas de alta, así como su desinfección en la consulta dental es de suma importancia, ya que nos ayudan a evitar contaminaciones cruzadas y a prevenir que dentro del área de trabajo se formen focos de infección (AU)


Health professionals are exposed to a wide variety of microorganisms from spores, bacteria, fungi, viruses and protozoa that can be found in the blood and/or saliva of patients. Any of these microorganisms can cause an infectious disease through punctures and / or splashes produced by the aerosol during dental practice.1,2 Objective: To determine the bacterial presence in the high-speed pieces used in clinical practice. Material and methods: It are an experimental, observational and transversal study; where the contamination of 30 high-speed pieces used in clinical practice was evaluated. Prior to the study, a standardization was made obtaining a kappa of 0.85. A database was made in the program SPSS version 22, with which the descriptive analysis was carried out to determine measures of central tendency. Results: 73.3% of the analyzed samples showed bacterial growth, among the bacteria that were found, 54.5% of them were gram-positive bacteria and the rest were gram-negative. The bacterium with the highest presence in the sample was for Bacillus in 45.5% followed by Streptococcus in 27.3%, the remaining 27.2% was for Staphylococcus, Coccus and Streptobacillus. Conclusions: The correct use of the discharging parts, as well as their disinfection in the dental practice is of the utmost importance as they help us to avoid cross contamination and to prevent foci of infection from forming within the work area (AU)


Subject(s)
Dental High-Speed Equipment/microbiology , Biological Contamination , Infection Control, Dental/methods , Colony Count, Microbial , Cross-Sectional Studies , Statistical Analysis , Culture Media , Dental Clinics/standards , Observational Study
20.
Prensa méd. argent ; 105(4): 192-196, jun 2019. tab, graf
Article in English | LILACS, BINACIS | ID: biblio-1026848

ABSTRACT

It has been found that the antagonistic activity of lactic acid bacteria depends on the composition of the nutrient medium and the temperature of culturing. It has been shown that the best antimicrobial effect to mycobacteria is achieved by the cultivation of lactic acid bacteria on the MRS nutrient media and a combined nutrient medium with the use of lactulose or glucose as a source of carbon. The optimum temperature for culturing an association of lactic acid bacteria for achieving high antagonistic activity to mycobacteria is 300C, and the duration of cultivation is 24 hours.


Subject(s)
Humans , Tuberculosis/etiology , Lactic Acid/antagonists & inhibitors , Probiotics/therapeutic use , Culture Media , Drug Resistance, Bacterial/immunology , Anti-Bacterial Agents/therapeutic use , Noxae
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