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1.
Lima; Perú. Ministerio de Salud; 20200900. 10 p.
Monography in Spanish | LILACS (Americas), LIPECS | ID: biblio-1118773

ABSTRACT

El documento contiene las pautas para garantizar la seguridad, calidad y desempeño en el desarrollo y validación de proyectos de investigación vinculados a los dispositivos de diagnóstico in vitro (DMDIVD), que permitan una autorización para la fabricación y uso.


Subject(s)
Research , Research Design , In Vitro Techniques , Coronavirus Infections , Diagnosis , Equipment and Supplies
2.
Goiânia; SES-GO; 12 ago 2020. 1-18 p. ilus, tab, graf, mapas.(Boletim Epidemiológico Covid-19, 19).
Monography in Portuguese | LILACS (Americas), ColecionaSUS, CONASS, SES-GO | ID: biblio-1117152

ABSTRACT

A Secretaria de Estado da Saúde de Goiás através da Superintendência de Vigilância em Saúde e Gerência de Vigilância Epidemiológica, apresentam neste boletim a distribuição de casos confirmados, óbitos e taxa de crescimento de COVID-19 no mundo, no Brasil, no período compreendido entre 31 de dezembro de 2019 a 08 de agosto de 2020. Quanto ao Estado de Goiás apresenta a distribuição dos casos notificados de COVID-19 segundo classificação e critério de confirmação, no período de 04 de fevereiro a 08 de agosto de 2020, a distribuição dos casos confirmados por data de início de sintomas, do acumulado de casos confirmados, dos casos confirmados e média móvel segundo a data de início de sintomas, do acumulado de óbitos, dos óbitos confirmados e média móvel segundo a data de ocorrência do óbito, da distribuição proporcional dos casos confirmados por municípios integrantes da Região metropolitana, do interior e capital, dos casos confirmados e taxa de incidência por município de residência, de casos confirmados por Semana Epidemiológica nas Macrorregiões, distribuição de casos confirmados de COVID-19 por Semana Epidemiológica segundo a região de saúde, número de casos confirmados segundo gênero, faixa etária e taxa de incidência, percentual de casos confirmados segundo raça/cor, casos confirmados segundo evolução, percentual de casos confirmados segundo ocupação, distribuição por município de residência dos óbitos confirmados segundo taxa de letalidade, percentual dos óbitos confirmados por sexo, óbitos confirmados e letalidade segundo faixa etária, casos confirmados que necessitaram de hospitalização segundo a evolução, perfil de gestantes com COVID-19, distribuição dos casos confirmados em gestantes segundo a necessidade de hospitalização, dos casos confirmados em gestantes segundo o local de internação, dos casos confirmados em gestantes segundo evolução, casos confirmados que necessitaram de hospitalização segundo o tempo médio de internação, percentual de casos confirmados por critério laboratorial segundo o método diagnóstico e positividade dos testes RT-PCR liberados pelo LACEN-GO segundo identificação de SARS-CoV-2


The Goiás State Department of Health, through the Health Surveillance Superintendence and Epidemiological Surveillance Management, presents in this bulletin the distribution of confirmed cases, deaths and growth rate of COVID-19 in the world, in Brazil, in the period between 31 from December 2019 to August 8, 2020. As for the State of Goiás, it presents the distribution of notified cases of COVID-19 according to classification and confirmation criteria, in the period from February 4 to August 8, 2020, the distribution of cases confirmed by date of onset of symptoms, cumulative number of confirmed cases, confirmed cases and moving average according to date of symptom onset, accumulated number of deaths, confirmed deaths and moving average according to date of occurrence of death, proportional distribution of confirmed cases by municipalities in the metropolitan region, inland and capital, confirmed cases and incidence rate by municipality of residence, d and confirmed cases by Epidemiological Week in Macroregions, distribution of confirmed cases of COVID-19 by Epidemiological Week according to health region, number of confirmed cases according to gender, age group and incidence rate, percentage of confirmed cases according to race / color, cases confirmed according to evolution, percentage of confirmed cases according to occupation, distribution by municipality of residence of deaths confirmed according to lethality rate, percentage of deaths confirmed by sex, confirmed deaths and lethality according to age group, confirmed cases that required hospitalization according to evolution, profile of pregnant women with COVID-19, distribution of confirmed cases in pregnant women according to the need for hospitalization, confirmed cases in pregnant women according to the place of hospitalization, confirmed cases in pregnant women according to the evolution, confirmed cases that required hospitalization according to the average length of hospitalization , percentage of trusted cases according to laboratory criteria according to the diagnostic method and positivity of the RT-PCR tests released by LACEN-GO according to the identification of SARS-CoV-2


Subject(s)
Humans , Male , Female , Pregnancy , Child , Adolescent , Adult , Middle Aged , Aged , Aged, 80 and over , Brazil/epidemiology , Incidence , Coronavirus Infections/epidemiology , Public Health Surveillance , Epidemiological Monitoring , Health Surveillance , Mortality , Disease Notification , Clinical Laboratory Techniques , Pregnant Women , Continental Population Groups , Diagnosis , Hospitalization , Age Groups
3.
Lima; Perú. Ministerio de Salud; 20200800. 184 p. ilus, tab.
Monography in Spanish | LILACS (Americas), LIPECS | ID: biblio-1117777

ABSTRACT

El documento Política Nacional Multisectorial de Salud al 2030, contiene los antecedentes, la base legal en la cual se sustenta, la metodología seguida, diagnóstico, los objetivos, provisión de servicios y estándares, para culminar en el seguimiento y la evaluación.


Subject(s)
Peru , Politics , Reference Standards , Health , Diagnosis , Methodology
4.
Arch. med ; 20(2): 331-343, 20200703.
Article in Portuguese | LILACS (Americas) | ID: biblio-1118851

ABSTRACT

Objetivo: a radiodermite ou radiodermatite é uma reação celular inflamatória desencadeada pela radioterapia, que é uma das alternativas para tratamento de vários tipos de cânceres. O objetivo deste trabalho foi analisar os principais métodos de diagnóstico de radiodermite com uso de ferramentas tecnológicas, quanto a equipamentos utilizados, forma de análise clínica, parâmetros desenvolvidos e produto final. Materiais e métodos: optou-se pela revisão sistemática de caráter exploratório e abordagem qualitativa. A busca por estudos em periódicos internacionais foi realizada no mês de julho de 2019, nas bases de dados MEDLINE e SCOPUS. Foram utilizados os descritores "radiodermatitis" e "acute radiation dermatitis", para a busca. Os critérios de inclusão foram: artigo, escrita em inglês, publicação nos últimos dez anos (01/2009 a 07/2019), e conteúdo relacionado ao diagnóstico de radiodermite causada por radioterapia de aplicação exclusiva. Resultados: esta revisão encontrou estudos sobre as seguintes ferramentas tecnológicas: avaliação citológica, avaliação de fluxo sanguíneo, machine learning, escala fotográfica, imagem térmica, dose de irradiação e espectrofotometria. O uso de imagens fotográficas e térmicas tem se consolidado como ferramenta de diagnóstico importante na área médica. Essas ferramentas tecnológicas têm se mostrado aplicáveis ao diagnóstico da radiodermite, com seu efeito potencializado por processamento digital de imagens e algoritmos de inteligência artificial. Conclusões: a análise aqui descrita demonstra a necessidade de desenvolvimento tecnológico para delineamento e padronização do processo de diagnóstico da radiodermite, o que permitiria tratamento precoce e manutenção do engajamento do indivíduo na terapia antineoplásica..Au


Objective: radiodermatitis or radiodermatitis is an inflammatory cell reaction triggered by radiotherapy, which is one of the alternatives for the treatment of various types of cancers. The objective of this work was to analyze the main diagnostic methods of radiodermatitis with the use of technological tools, regarding the equipment used, the form of clinical analysis, parameters developed and the final product. Materials and methods: a systematic review with an exploratory nature and a qualitative approach was performed. The search for studies in international journals was carried out in July 2019, in the MEDLINE and SCOPUS databases. The descriptors "radiodermatitis" and "acute radiation dermatitis" were used for the search. The inclusion criteria were: article, written in English, published in the last ten years (01/2009 to 07/2019), and content related to the diagnosis of radiodermatitis caused by radiotherapy of exclusive application. Results: This review found studies on the following technological tools:cytological assessment, blood flow assessment, machine learning, photographic scale, thermal image, irradiation dose, and spectrophotometry. The use of photographic and thermal images has been consolidated as an important diagnostic tool in the medical field. These technological tools have been shown to be applicable to the diagnosis of radiodermatitis, with its effect enhanced by digital image processing and artificial intelligence algorithms.Conclusions: The analysis described here demonstrates the need for technological development to outline and standardize the radiodermatitis diagnostic process, which would allow early treatment and maintenance of the individual's engagement in antineoplastic therapy..Au


Subject(s)
Radiodermatitis , Diagnosis
5.
Cuad. Hosp. Clín ; 61(1): [11], jul. 2020. ilus.
Article in Spanish | LILACS (Americas), LIBOCS | ID: biblio-1118902

ABSTRACT

Los tumores de células gigantes (TDG) llamados también osteoclastomas o tumores pardos cuando se hallan dentro la esfera endocrinológica, son uno de los tumores menos frecuentes, más controversial y menos predecible en su comportamiento. Se producen como consecuencia del exceso de la actividad osteoclastica, como ocurre en el caso del hiperparatiroidismo, mismo que es un desorden endocrino común, por lo general asintomático y diagnosticado por el hallazgo fortuito de hipercalcemia. El diagnóstico de los osteoclastomas suele ser un reto, el alto índice de sospecha es esencial y la biopsia es el estándar de oro para el diagnóstico. Presentamos el caso de un hombre de 42 años quien presentó fracturas patológicas de radio derecho y tibia izquierda, gammagrama óseo con Tc ­ 99m con múltiples lesiones óseas , hormona paratiroidea (PTH) elevada, hipercalcemia, gammagrama de paratiroides con MIBI con presencia de adenoma paratiroideo, la biopsia de las lesiones óseas con presencia de células gigantes multinucleadas correspondientes a osteoclastomas; se llevó a cabo paratiroidectomia y el examen histopatológico confirmó la presencia de un adenoma paratiroideo.


Giant cell tumors (TDG), also called osteoclastomas or brown tumors when they are within the endocrinological sphere, are one of the least frequent, most controversial and least predictable tumors in their behavior. They occur as a consequence of excess osteoclastic activity, as occurs in the case of hyperparathyroidism, which is a common endocrine disorder, generally asymptomatic and diagnosed by the fortuitous finding of hypercalcemia. Diagnosing osteoclastomas is usually challenging, the high index of suspicion is essential, and biopsy is the gold standard for diagnosis. We present the case of a 42-year-old man who presented pathological fractures of the right radius and left tibia, a bone scan with Tc-99m with multiple bone lesions, elevated parathyroid hormone (PTH), hypercalcemia, parathyroid scan with MIBI with the presence of parathyroid adenoma, the biopsy of the bone lesions with the presence of multinucleated giant cells corresponding to osteoclastomas; parathyroidectomy was performed and histopathological examination confirmed the presence of a parathyroid adenoma.


Subject(s)
Male , Adult , Wounds and Injuries , Biopsy , Diagnosis , Hypercalcemia
6.
Ludovica pediátr ; 23(1): 11-19, marzo 2020.
Article in Spanish | LILACS (Americas), BINACIS | ID: biblio-1102850

ABSTRACT

A partir de la sanción de la ley Nacional N.º 27447, conocida popularmente como "Ley Justina", se genera un nuevo Protocolo para el diagnóstico de cese irreversible de las funciones encefálicas. Nuestro Hospital, a través del servicio de Terapia Intensiva, tuvo la oportunidad de participar en su confección.El protocolo consta de criterios de inclusión, periodos de evaluación, métodos auxiliares y la consideración de cómo arribar al diagnóstico en diferentes situaciones especiales.Los cambios más importantes se realizaron en la edad pediátrica, donde se logró acortar los tiempos de evaluación para arribar a un diagnóstico de certeza, acortando la espera familiar


From the enactment of National Law No. 27447, popularly known as the Justina law, a new Protocol for the Diagnosis of Irreversible Cessation of Encephalic Functions was created. Our Hospital, through the Intensive Care Service, had the opportunity to participate in its preparation.The protocol consists of inclusion criteria, evaluation periods, ancillary methods and the consideration of how to arrive at the diagnosis in different special situations.The most important changes were made in the pediatric age where the evaluation times were shortened to arrive at a diagnosis of certainty, shortening the family wait


Subject(s)
Pediatrics , Diagnosis , Brain Death
9.
Rev. psicanal ; 27(1): http://revista.sppa.org.br/index.php/RPdaSPPA/article/view/538, Abril 2020.
Article in Portuguese | LILACS (Americas) | ID: biblio-1117967

ABSTRACT

O predomínio dos modelos ideais no momento de estabelecer as metas do trabalho psicanalítico leva a uma série de paradoxos, ou polaridades, apontada em 1965 por R. Wallerstein (existência/não existência de metas na análise, metas relativas ao processo/metas relativas aos resultados, visões otimistas/pessimistas sobre as metas). Estas polaridades são cotejadas com posições similares, existentes na mesma época no Rio da Prata (J. Bleger discutido por D. Liberman e C. A. Paz). A evolução posterior das ideias mostra a emergência de novas oscilações quando se tenta definir as metas a partir do conceito de processo psicanalítico. Ditas dificuldades sugerem a conveniência de prestar mais atenção à variedade de tratamentos que nós, analistas, realizamos em nossa prática real e aos resultados que efetivamente alcançamos neles. Estas ideias são examinadas a partir de um caso clínico cujo tratamento se afasta da análise clássica. Descrevem-se os diferentes tipos de metas que apareceram de forma progressiva para o analista durante as sessões e o papel que desempenharam. De modo mais geral, discute-se a natureza das metas dos tratamentos analíticos e a sua relação com as características do processo psicanalítico (AU)


This chapter VIII reviews the concept of neurosis in the 21st Century. Major concept of Freudian metapsychology based upon the discoveries of infantile sexuality and of infantile sexual theories, neurosis disappeared from the DSM in 2014. Infantile neurosis, transference neurosis, actual neurosis or narcissistic neurosis are concepts void of diagnostic value from the vantage point of the psychiatric nosography. Conflict between the Ego and the Id, as the economic balance between primary and secondary defense mechanisms, are no longer the major lenses to observe the psychic functioning. The author examines the influence of social and developmental modifications of the western 21st Century ­ such as the disappearance of the latency period, and the inflation of information concerning culture ­ on the situation of the concept of neurosis today. Taken out of its core situation, where Freud put it, neurosis is described as a situation of opposition in the concept of the 3rd type, placing neurosis opposite to not only perversion, but also to psychosis, or else, to psychopathy (AU)


El artículo hace un balance del concepto de neurosis en el siglo XXI. Concepto importante en la metapsicología freudiana desde el descubrimiento de la sexualidad infantil y las teorías sexuales infantiles, la neurosis desapareció en 2014 del repertorio del DSM. La neurosis infantil, la neurosis de transferencia, la neurosis actual o incluso la neurosis narcisista son conceptos vaciados de su valor diagnóstico desde el punto de vista de la nosografía psiquiátrica. El conflicto entre el Ego y el Ello, como el equilibrio económico entre los mecanismos de defensa primario y secundario, ya no son los ejes principales de observación del funcionamiento psíquico. La autora examina la influencia de los cambios sociales y de desarrollo en el siglo XXI occidental ­ como el fin del período de latencia y la inflación de información en relación con la cultura ­ sobre el estado actual del concepto de neurosis. Desplazada de la situación central en la que Freud la había colocado, la neurosis es descrita en una situación de polaridad en conceptos del tercer tipo que oponen la neurosis no solo a la perversión, sino también a la psicosis e incluso a la psicopatía (AU)


Subject(s)
Ego , Id , Neurotic Disorders , Psychotic Disorders , Diagnosis
10.
Lima; Perú. Poder Ejecutivo; 20200300. 3 p.
Non-conventional in Spanish | LILACS (Americas), LIPECS | ID: biblio-1116591

ABSTRACT

El Decreto contiene las medidas adicionales extraordinarias que permitan adoptar las acciones preventivas y de respuesta para reducir el riesgo de propagación y el impacto sanitario de la enfermedad causada por el virus del COVID-19, en el territorio nacional, con la finalidad de reforzar los sistemas de prevención, control, vigilancia y respuesta sanitaria, preservar la salud y el empleo de los trabajadores; y de esta forma coadyuvar a disminuir la afectación de la economía peruana por la propagación del mencionado virus a nivel nacional.


Subject(s)
Immunologic Tests , Coronavirus Infections , Surveillance , Decrees , Diagnosis , Control
11.
Biosci. j. (Online) ; 36(1): 78-86, jan./feb. 2020. graf
Article in English | LILACS (Americas) | ID: biblio-1049198

ABSTRACT

The root-knot nematode (Meloidogyne spp.) is the most important plant-parasitic nematode genus, they are the most common and destructive pathogens in this group. They produce some of the most drastic symptoms in plants and can significantly reduce the yield of crops. In order to achieve deploy an efficient method of plant-parasitic nematode management, is necessary an identification and quantification accurate and reliable of plant-parasitic nematodes. The aim of this study was to analyze samples in qPCR to detect and quantify M. incognita, in the field samples, comparing different methods of extraction of DNA and its efficacy in establishing the number of individuals. For this purpose the effectiveness of different DNA methods of extraction was compared through the values of CT intervals. For standard curve and method comparisons, we used nematodes multiplied in a greenhouse and carefully separated in the specific quantities of the experiments. For the number of individuals experiment field samples previously counted under an optical microscope were used. The DNA extraction was made from 100 nematodes by the methods: CTAB, Phenol: Chloroform and commercial kit (PureLink® Genomic DNA Kit, Invitrogen). In the comparative analysis using the three methods of DNA extracting from 100 nematodes, it was observed that commercial kit and CTAB methods obtained CT values similar. The CTAB method of extraction, showed less variation in the repeats and greater linearity of standard curve in comparison with other methods tested. So, it was possible to quantify the samples through the CT value intervals, established from different numbers of individuals (1, 10, 25, 100, 250, 500 and 750), in field samples. This study demonstrated that qPCR technique is an alternative sensitive and reliable for the quantification of M. incognita to support laboratories of diagnose and field survey.


Os nematoides-das-galhas (Meloidogyne spp.) é o gênero de fitonematoide mais importante, são os patógenos mais comuns e destrutivos deste grupo. Eles produzem alguns dos sintomas mais drásticos nas plantas e podem reduzir significativamente o rendimento das culturas. Para conseguir implantar um método eficiente de manejo de nematoides parasitas de plantas, é necessária a identificação e quantificação precisa e confiável dos fitonematoides. O objetivo deste estudo foi analisar amostras em qPCR para detectar e quantificar M. incognita, em amostras de campo, comparando diferentes métodos de extração do DNA e sua eficácia no estabelecimento do número de indivíduos. Para este propósito, a eficácia de diferentes métodos de extração de DNA foi comparada através dos valores dos intervalos de Ct. Para comparações padrão de curvas e métodos, usamos nematoides multiplicados em casa de vegetação e cuidadosamente separados nas quantidades específicas dos experimentos. Para o número de indivíduos, foram utilizadas amostras de campo previamente contadas sob um microscópio óptico. A extração de DNA foi realizada a partir de 100 nematoides, pelos métodos: CTAB, Phenol: Clorofórmio e kit comercial (PureLink® Genomic DNA Kit, Invitrogen). Na análise comparativa utilizando os três métodos de extração de DNA a partir de 100 nematoides, observou-se que o kit comercial e os métodos de CTAB obtiveram valores de CT semelhantes. O método de extração CTAB apresentou menor variação nas repetições e maior linearidade da curva padrão em comparação com os demais métodos testados. O coeficiente de correlação (R2) da curva padrão foi de 0,98 indicando uma relação linear entre o valor de Ct e a quantidade de padrões de DNA variando de 90 a 0,00009 ng.µL-1. Assim, foi possível quantificar as amostras através dos intervalos de valores de CT, estabelecidos a partir de diferentes números de indivíduos (1, 10, 25, 100, 250, 500 e 750), em amostras de campo. Este estudo demonstrou que a técnica de qPCR é uma alternativa sensível e confiável para a quantificação de M. incognita, para apoiar laboratórios de diagnóstico e levantamentos de campo.


Subject(s)
Tylenchoidea , Monitoring , Diagnosis , Nematoda , Plant Tumors
12.
Braz. dent. sci ; 23(1): 1-5, 2020. ilus
Article in English | LILACS (Americas), BBO | ID: biblio-1050102

ABSTRACT

Ameloblastoma is an odontogenic tumor that affects the bones of the jaw, often affecting posterior region of the mandible. It is a benign neoplasm and is frequently associated to an unerupted tooth. The purpose of this article is to report a case of failure in making an immediate full denture in a patient that had an ameloblastoma. A female patient aged 67 years complained of a hard swelling in the right mandible at the premolar region, that she noted after teeth extraction and confection of an immediate denture. Intraoral examination showed buccal and lingual cortical plate expansion and radiograph examination showed multilocular radiolucency with a well-defined margin. Aspiration was nonproductive and the provisional diagnosis was ameloblastoma. An incisional biopsy was performed and the histopathological report was conclusive of an acanthomatous ameloblastoma. The patient was sent for surgical excision of the lesion and after three years, the patient returned reporting that she was operated elsewhere in the past year and had an unsuccessful bone graft. She was sent to a prosthodontist to make a complete denture. In the present case the ameloblastoma was diagnosed only after the teeth extraction and immediate denture (AU)


O ameloblastoma é um tumor odontogênico que afeta os ossos maxilares, geralmente a região posterior de mandíbula. É uma neoplasia benigna frequentemente associada a um dente não irrompido. O objetivo deste artigo é relatar um caso de prótese total imediata confeccionada para uma paciente com ameloblastoma na região anterior da mandíbula. Paciente do sexo feminino, 67 anos, queixou-se de aumento de volume na região de pré-molares inferiores do lado direito após extração dentária e confecção de prótese total imediata. Ao exame clínico intrabucal foi observada expansão da cortical vestibular e lingual e o exame radiográfico revelou radiolucência multilocular com margem bem definida. A punção aspirativa não foi produtiva e o diagnóstico provisório foi de ameloblastoma. Foi realizada biópsia incisional e o laudo histopatológico foi conclusivo para ameloblastoma acantomatoso. A paciente foi encaminhada para ressecção cirúrgica da lesão e não retornou. Depois de três anos procurou atendimento e relatou que tinha sido submetida a cirurgia com colocação de enxerto ósseo para implante sem sucesso. O enxerto ósseo foi perdido e a paciente necessitava de nova prótese total. Ela foi encaminhada a um protesista. No presente caso, o diagnóstico de ameloblastoma foi feito somente após a instalação da prótese total imediata, quando a paciente notou aumento de volume (AU).


Subject(s)
Humans , Female , Aged , Ameloblastoma , Mandibular Neoplasms , Odontogenic Tumors , Diagnosis
13.
Article in English | WPRIM (Western Pacific) | ID: wprim-820815

ABSTRACT

Alzheimer's disease (AD) is pathologically characterized by a long progressive phase of neuronal changes, including accumulation of extracellular amyloid-β (Aβ) and intracellular neurofibrillary tangles, before the onset of observable symptoms. Many efforts have been made to develop a blood-based diagnostic method for AD by incorporating Aβ and tau as plasma biomarkers. As blood tests have the advantages of being highly accessible and low cost, clinical implementation of AD blood tests would provide preventative screening to presymptomatic individuals, facilitating early identification of AD patients and, thus, treatment development in clinical research. However, the low concentration of AD biomarkers in the plasma has posed difficulties for accurate detection, hindering the development of a reliable blood test. In this review, we introduce three AD blood test technologies emerging in South Korea, which have distinctive methods of heightening detection sensitivity of specific plasma biomarkers. We discuss in detail the multimer detection system, the self-standard analysis of Aβ biomarkers quantified by interdigitated microelectrodes, and a biomarker ratio analysis comprising Aβ and tau.


Subject(s)
Alzheimer Disease , Biomarkers , Diagnosis , Diagnostic Techniques and Procedures , Hematologic Tests , Humans , Korea , Mass Screening , Methods , Microelectrodes , Neurofibrillary Tangles , Neurons , Plasma , tau Proteins
14.
Article in English | WPRIM (Western Pacific) | ID: wprim-816637

ABSTRACT

Sexually transmitted infections (STIs) are caused by the spread of pathogens via sexual activity and can cause serious complications if left untreated, regardless of their symptoms. Therefore, early diagnosis of STI is important, and molecular diagnostic methods for rapid detection and monitoring are needed. In this study, we evaluated a multiplex polymerase chain reaction (PCR) kit for simultaneously detecting 13 different bacterial, fungal, and viral microorganisms that cause STIs. The kit performance was evaluated for its sensitivity, lot-to-lot variation, and interference in detecting different pathogens. Additionally, its clinical usefulness was evaluated by estimating its sensitivity and specificity for clinical samples. The limit of detection (LOD) was 0.021–50.104 copies for each pathogen. In the tests of lot-to-lot, 100% of positive samples were detected at low concentrations and negative samples all showed negative results. This result confirms that there is no the variation of lot-to-lot. In the test for interference between pathogens, the efficiency of amplification for each pathogen was not significantly reduced and no nonspecific amplification product was formed. We tested 322 vaginal swab samples using the multiplex PCR kit and confirmed that its clinical sensitivity and specificity were 100% for all pathogens. This multiplex PCR kit can be used widely for rapid diagnosis and monitoring of STIs.


Subject(s)
Diagnosis , Early Diagnosis , Limit of Detection , Multiplex Polymerase Chain Reaction , Pathology, Molecular , Sensitivity and Specificity , Sexual Behavior , Sexually Transmitted Diseases
15.
Article in English | WPRIM (Western Pacific) | ID: wprim-816632

ABSTRACT

Adrenal masses are mainly detected unexpectedly by an imaging study performed for reasons unrelated to any suspect of adrenal diseases. Such masses are commonly defined as “adrenal incidentalomas” and represent a public health challenge because they are increasingly recognized in current medical practice. Management of adrenal incidentalomas is currently matter of debate. Although there is consensus on the need of a multidisciplinary expert team evaluation and surgical approach in patients with significant hormonal excess and/or radiological findings suspicious of malignancy demonstrated at the diagnosis or during follow-up, the inconsistency between official guidelines and the consequent diffuse uncertainty on management of small adrenal incidentalomas still represents a considerable problem in terms of clinical choices in real practice. The aim of the present work is to review the proposed strategies on how to manage patients with adrenal incidentalomas that are not candidates to immediate surgery. The recent European Society of Endocrinology/European Network for the Study of Adrenal Tumors guidelines have supported the view to avoid surveillance in patients with clear benign adrenal lesions <4 cm and/or without any hormonal secretion; however, newer prospective studies are needed to confirm safety of this strategy, in particular in younger patients.


Subject(s)
Adrenal Gland Neoplasms , Consensus , Cushing Syndrome , Diagnosis , Endocrinology , Follow-Up Studies , Humans , Practice Management, Medical , Prospective Studies , Public Health , Uncertainty
16.
Article in English | WPRIM (Western Pacific) | ID: wprim-816617

ABSTRACT

BACKGROUND: Pheochromocytomas and paragangliomas (PPGLs) are rare endocrine tumors originating from chromaffin cells. PPGLs are associated with a high mortality rate and several complications. To date, no epidemiological studies have been conducted on PPGLs in Asia. This study aimed to investigate the epidemiology and prognosis of PPGLs in Korea using nationwide data.METHODS: Using the National Health Insurance Service Database, subjects with a principal diagnosis of PPGLs on two or more occasions between 2003 and 2014 who satisfied the operational definition of PPGLs were included. Incidence, prevalence, complications, metastasis, and mortality were investigated.RESULTS: In total, 1048 subjects with a mean age of 47.6±16.1 years were included. There was no sex preponderance. The overall prevalence of PPGLs was 2.13 per 100,000 persons, and the overall age-standardized incidence rate was 0.18 per 100,000 person-years. Malignant PPGLs accounted for 17.7% (185 of 1,048) of cases, and 94 subjects exhibited metastasis at the time of diagnosis. Among initially non-metastatic PPGLs, 9.5% (nine of 954) eventually metastasized after a mean duration of 78.1±41.4 months. The 5-year survival rates for non-metastatic and metastatic PPGLs at diagnosis were 97% and 84%, respectively. Multivariable Cox regression models adjusted for covariates showed that metastatic PPGLs were associated with a 2.40-fold higher risk of mortality than non-metastatic PPGLs (95% confidence interval, 1.38 to 4.17; P=0.002).CONCLUSION: PPGLs are rare in Korea, and the prognosis of these endocrine tumors varies depending on whether they are benign or malignant. This epidemiological study paves the way for further research on PPGLs.


Subject(s)
Asia , Chromaffin Cells , Diagnosis , Epidemiologic Studies , Epidemiology , Humans , Incidence , Korea , Mortality , National Health Programs , Neoplasm Metastasis , Paraganglioma , Pheochromocytoma , Prevalence , Prognosis , Survival Rate
17.
Article in English | WPRIM (Western Pacific) | ID: wprim-816614

ABSTRACT

X-linked adrenoleukodystrophy (X-ALD) occurs due to mutations in the ABCD1 gene that encodes the peroxisomal membrane protein peroxisomal transporter ATP-binding cassette sub-family D member 1 (ABCD1). Degradation of very long-chain fatty acids in peroxisomes is impaired owing to ABCD dysfunction, subsequently leading to adrenomyeloneuropathy, cerebral adrenoleukodystrophy, and adrenal insufficiency. X-ALD frequently induces idiopathic Addison's disease in young male patients. Here, we confirmed the diagnosis of X-ALD in a young male patient with primary adrenal insufficiency, and identified a novel ABCD1 gene mutation (p.Trp664*, c.1991 G>A).


Subject(s)
Addison Disease , Adrenal Insufficiency , Adrenoleukodystrophy , Diagnosis , Fatty Acids , Humans , Male , Membrane Proteins , Peroxisomes
18.
Article in Korean | WPRIM (Western Pacific) | ID: wprim-816607

ABSTRACT

BACKGROUND: 16S rRNA gene-targeted next-generation sequencing (NGS) can detect microorganisms in a comprehensive reference database. To date, NGS has been successfully applied to samples such as urine, blood, and synovial fluid. However, there is no data for continuous ambulatory peritoneal dialysis (CAPD) fluid. The purpose of this study was to evaluate the clinical usefulness of microbiome analysis of CAPD fluids for the diagnosis of CAPD peritonitis.METHODS: We included 21 patients with high suspicion of CAPD peritonitis. Routine CAPD fluid culture was performed using a pellet of 50 mL CAPD fluid onto the chocolate and blood agar for two days, and thioglycollate broth for one week. 16S rRNA gene-targeted NGS of pellets, stored at −70℃ was performed with MiSeq (Illumina, USA).RESULTS: Many colonized or pathogenic bacteria were detected from CAPD fluids using NGS and the microbiomes were composed of 1 to 29 genera with a cut-off 1.0. Compared to the culture results, NGS detected the same pathogens in 6 of 18 valid results (three samples failed with low read count). Additionally, using NGS, anaerobes such as Bacteroides spp. and Prevotella spp. were detected in six patients. In two of five samples in which no bacterial growth was detected, possible pathogens were detected by NGS.CONCLUSION: To our knowledge, this is the first report about the application of 16S rRNA gene-targeted NGS for diagnosis of CAPD peritonitis. Etiology of culture-negative CAPD peritonitis can be better defined in NGS. Furthermore, it also helped the detection of anaerobic bacteria.


Subject(s)
Agar , Bacteria , Bacteria, Anaerobic , Bacteroides , Cacao , Colon , Diagnosis , Humans , Microbiota , Peritoneal Dialysis, Continuous Ambulatory , Peritonitis , Prevotella , Synovial Fluid
19.
Article in English | WPRIM (Western Pacific) | ID: wprim-762186

ABSTRACT

MicroRNAs (miRs) are single-stranded RNAs of 18-25 nucleotides. These molecules regulate gene expression at the post-transcriptional level; several of these are differentially expressed in asthma as well as in viral acute respiratory infections (ARIs), the main triggers of acute asthma exacerbations. In recent years, miRs have been studied in order to discover drug targets as well as biomarkers for diagnosis, disease severity and prognosis. We describe recent findings on miR expression and function in asthma and their role in the regulation of viral ARIs, according to cell tissue specificity and asthma severity. By combining the above information, we identify miRs that may be important in virus-induced asthma exacerbations. This is the first attempt to link miR profiles of asthmatic patients and ARI-induced miRs, addressing the question of whether there might be a specific miR deficit in asthmatic subjects that make them more susceptible and/or reactive to infection.


Subject(s)
Asthma , Biomarkers , Diagnosis , Disease Progression , Gene Expression , Humans , Inflammation , MicroRNAs , Nucleotides , Organ Specificity , Prognosis , Respiratory Tract Infections , RNA
20.
Yonsei Medical Journal ; : 257-261, 2020.
Article in English | WPRIM (Western Pacific) | ID: wprim-811469

ABSTRACT

Esthesioneuroblastoma as a source of ectopic Cushing's syndrome is rare, and to the best of our knowledge, only 20 cases have been reported worldwide. A 46-year-old healthy man visited a local clinic for general weakness and hyposmia, and underwent examination with serial endocrinological workup and brain imaging. ⁶⁸Gallium-DOTA-TOC positron emission tomography scan was helpful where diagnosis of sellar MRI and inferior petrosal sinus sampling were discordant. Combined transcranial and endoscopic endonasal approach surgery was performed, and a diagnosis of esthesioneuroblastoma was given.


Subject(s)
Cushing Syndrome , Diagnosis , Esthesioneuroblastoma, Olfactory , Humans , Magnetic Resonance Imaging , Middle Aged , Neuroimaging , Petrosal Sinus Sampling , Positron-Emission Tomography
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