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1.
Hig. Aliment. (Online) ; 37(297): e1122, jul.-dez. 2023. ilus, tab
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1531976

ABSTRACT

Várias pesquisas mostram o interesse na aplicação de materiais de revestimento na superfície de cascas de ovos para preservar a qualidade interior dos ovos, bem como para prolongar sua vida útil. No presente trabalho verificou-se a qualidade interna de 100 ovos galinhas para o consumo. Os ovos foram revestidos externamente na casca com tratamento de óleo mineral e timol 10%, e um grupo controle, que não sofreu nenhum tipo de tratamento. Um total de 10 ovos de cada grupo (controle e tratado) foram avaliados no dia 7, 14, 21 dias de armazenamento em relação aos seus parâmetros internos de qualidade. Os parâmetros físico-químicos de qualidade dos ovos avaliados foram Perda de peso dos ovos (g), Porcentagem da gema (%), Porcentagem do albúmen (%), Índice de gema, pH do Albúmen, pH da gema e Unidades Haugh (UH). Foi observada diminuição da qualidade interna dos ovos durante os períodos de armazenamento principalmente no grupo controle. Os ovos do grupo controle, que não receberam nenhum tipo de revestimento apresentaram a maior perda de peso e os valores da unidade Haugh diminuíram ao longo do armazenamento, o que não foi observado nos dois grupos tratados. A unidade Haugh dos grupos tratados não apresentaram diferenças significativas entre si. Concluiu-se que os ovos tratados com óleo mineral e timol a 10%, o revestimento da casca contribuiu para a preservação dos ovos mantendo positivamente a qualidade interna dos ovos ao longo do período de estocagem, o que pode contribuir para o aumento da vida de prateleira de ovos.


Several researchers show interest in the application of coating materials on the surface of eggshells to preserve the interior quality of the eggs, as well as to prolong their shelf life. In the present work, the internal quality of 100 chicken eggs for consumption was verified. The eggs were coated externally in the shell with mineral oil and 10% thymol treatment, and a control group did not undergo any type of treatment. a total of 10 eggs from each group (control and treated) were evaluated on day 7, 14, 21 of storage in relation to their internal quality parameters. The physicochemical parameters of egg quality evaluated were egg weight loss (g), yolk percentage (%), albumen percentage (%), yolk index, albumen pH, yolk ph and haugh units (uh). decreased internal egg quality was observed during storage periods, mainly in the control group. The eggs in the control group, which did not receive any kind of coating, showed the greatest weight loss and the haugh unit values decreased during storage, which was not observed in the two treated groups. The haugh unit of the treated groups did not show significant differences between them. It was concluded that in eggs treated with mineral oil and 10% thymol, the shell coating contributed to the preservation of the eggs, positively maintaining the internal quality of the eggs throughout the storage period, which may contribute to an increase in shelf life of eggs.


Subject(s)
Chickens , Egg Shell , Eggs , Date of Validity of Products , Thymol , Mineral Oil
2.
Chinese Journal of Reparative and Reconstructive Surgery ; (12): 1489-1495, 2023.
Article in Chinese | WPRIM | ID: wpr-1009088

ABSTRACT

OBJECTIVE@#To explore the safety and effectiveness of one-stage posterior eggshell osteotomy and long-segment pedicle screw fixation in the treatment of ankylosing spondylitis kyphosis combined with acute thoracolumbar vertebral fracture.@*METHODS@#A clinical data of 20 patients with ankylosing spondylitis kyphosis combined with acute thoracolumbar spine fracture, who were treated with one-stage posterior eggshell osteotomy and long-segment pedicle screw fixation between April 2016 and January 2022, was retrospectively analyzed. Among them, 16 cases were male and 4 cases were female; their ages ranged from 32 to 68 years, with an average of 45.9 years. The causes of injury included 10 cases of sprain, 8 cases of fall, and 2 cases of falling from height. The time from injury to operation ranged from 1 to 12 days, with an average of 7.1 days. The injured segment was T 11 in 2 cases, T 12 in 2 cases, L 1 in 6 cases, and L 2 in 10 cases. X-ray film and CT showed that the patients had characteristic imaging manifestations of ankylosing spondylitis, and the fracture lines were involved in the anterior, middle, and posterior columns and accompanied by different degrees of kyphosis and vertebral compression; and MRI showed that 12 patients had different degrees of nerve injuries. The operation time, intraoperative bleeding, intra- and post-operative complications were recorded. The visual analogue scale (VAS) score and Oswestry disability index (ODI) were used to evaluate the low back pain and quality of life, and the American spinal cord injury association (ASIA) classification was used to evaluate the neurological function. X-ray films were taken, and local Cobb angle (LCA) and sagittal vertical axis (SVA) were measured to evaluate the correction of the kyphosis.@*RESULTS@#All operations were successfully completed and the operation time ranged from 127 to 254 minutes (mean, 176.3 minutes). The amount of intraoperative bleeding ranged from 400 to 950 mL (mean, 722.5 mL). One case of dural sac tear occurred during operation, and no cerebrospinal fluid leakage occurred after repair, and the rest of the patients did not suffer from neurological and vascular injuries, cerebrospinal fluid leakage, and other related complications during operation. All incisions healed by first intention without infection or fat liquefaction. All patients were followed up 8-16 months (mean, 12.5 months). The VAS score, ODI, LCA, and SVA at 3 days after operation and last follow-up significantly improved when compared with those before operation ( P<0.05), and the difference between 3 days after operation and last follow-up was not significant ( P>0.05). The ASIA grading of neurological function at last follow-up also significantly improved when compared with that before operation ( P<0.05), including 17 cases of grade E and 3 cases of grade D. At last follow-up, all bone grafts achieved bone fusion, and no complications such as loosening, breaking of internal fixation, and pseudoarthrosis occurred.@*CONCLUSION@#One-stage posterior eggshell osteotomy and long-segment pedicle screw fixation is an effective surgical procedure for ankylosing spondylitis kyphosis combined with acute thoracolumbar vertebral fracture. It can significantly relieve patients' clinical symptoms and to some extent, alleviate the local kyphotic deformity.


Subject(s)
Humans , Male , Female , Animals , Adult , Middle Aged , Aged , Spinal Fractures/surgery , Pedicle Screws , Spondylitis, Ankylosing/surgery , Quality of Life , Retrospective Studies , Egg Shell/injuries , Thoracic Vertebrae/injuries , Lumbar Vertebrae/injuries , Kyphosis/surgery , Osteotomy , Fracture Fixation, Internal/methods , Fractures, Compression/surgery , Treatment Outcome
3.
Rev. bras. oftalmol ; 81: e0040, 2022. graf
Article in English | LILACS | ID: biblio-1376790

ABSTRACT

ABSTRACT Purpose: To describe a new accessible model of ophthalmological training using chicken eggs. Methods: With the aid of a spherical drill, the external calcified layer and the cuticle of the chicken eggshell were removed in a 2cm diameter circle. Using a video-magnification system, the film was dissected and cut to approximately 1.5 cm diameters. The film was removed and repositioned to make interrupted 12-0 nylon microsutures. The parameters analyzed were: cost, facility of acquisition and handling, time for making the model and the microsutures and number of possible uses. Results: In all simulators, it was possible to carry out separated and equidistant micro-sutures in the egg membrane, without the need for reintervention. Conclusion: The new chicken-egg model for ophthalmic surgery training is low-cost, easy to acquire and handle, and viable for the development of basic microsurgery skills.


RESUMO Objetivo: Descrever um novo modelo acessível de treinamento oftalmológico com ovo de galinha. Métodos: Com o auxílio de uma broca esférica, a camada calcificada externa e a cutícula da casca do ovo de galinha foram retiradas em um círculo 2 cm de diâmetro. Mediante um sistema de videomagnificação, a película foi dissecada e cortada em formato aproximado de 1,5 cm de diâmetro. A película foi removida e reposicionada para a confecção de microssuturas interrompidas com nylon 12-0. Os parâmetros analisados foram: custo, facilidade de aquisição e manuseio, tempo para confecção do modelo e para a confecção das microssuturas e número de utilizações possíveis. Resultados: Em todos os simuladores foi possível realizar microssuturas separadas e equidistantes na membrana do ovo, sem necessidade de reintervenção. Conclusão: O novo modelo com ovo de galinha para o treinamento de cirurgia oftalmológica é de baixo custo, fácil aquisição e manuseio, além de ser viável no desenvolvimento de habilidades básicas em microcirurgia.


Subject(s)
Animals , Ophthalmologic Surgical Procedures/education , Egg Shell , Simulation Training/methods , Microsurgery/education , Models, Anatomic , Ophthalmology/education , Chickens , Suture Techniques/education , Eggs
4.
Rev. cuba. med. mil ; 49(4): e718,
Article in Spanish | CUMED, LILACS | ID: biblio-1156531

ABSTRACT

La membrana de cáscara de huevo de gallina es un producto rico en colágeno, ácido hialurónico, condroitina, glucosamina, queratina y lisozima. Activa el desarrollo de los fibroblastos presentes en la piel, los cuales producen colágeno tipo III. Diseñar un apósito que prevenga la infección bacteriana, que sea resistente a múltiples fármacos, que promueva la angiogénesis y la reepitelización es de gran importancia para el tratamiento de heridas. Por ello, las investigaciones recientes han sugerido el uso de la membrana de cáscara de huevo para favorecer la curación de heridas como terapia innovadora.1 En el estudio de Liu M y otros,1 realizado en 2019 se desarrolló una membrana compuesta biocompatible, con microfibras de cáscara de huevo modificadas con polidopamina biomimética, recubiertas con péptido antimicrobiano KR-12 y ácido hialurónico (HA). Las propiedades fisicoquímicas de la membrana compuesta, se caracterizaron completamente y los resultados mostraron que la hidrofilia superficial y la capacidad de absorción de agua mejoraron después de la conjugación sucesiva del HA y el péptido KR-12. Además, los resultados revelaron que la membrana compuesta tenía una excelente actividad antibacteriana contra Staphylococcus aureus, Staphylococcus aureus resistente a meticilina (MRSA, por sus siglas en inglés), Escherichia coli y podía prevenir la formación de biopelícula MRSA en su superficie. Cabe destacar que no se refiere a un componente de la biopelícula, sino un mecanismo muy propio de resistencia a los antibióticos betalactámicos. También promovió la proliferación de queratinocitos y células endoteliales. Por tanto, esta membrana podría promover la cicatrización de heridas a través de la aceleración de la angiogénesis y la reepitelización, lo cual se demostró mediante la expresión mejorada de marcadores angiogenéticos y el marcador de proliferación de queratinocitos. En el estudio de Guarderas F y otros,2 realizado en 2016, se examinó la eficacia del vendaje de membrana de huevo de gallina en la cicatrización de heridas. Este estudio fue realizado en ratas. Cada animal recibió 2 heridas en la parte superior de la espalda. Una herida no fue tratada y la otra estaba vestida con membrana de huevo de gallina. La mitad de las ratas recibió tratamiento con membrana de huevo en la herida inferior, mientras que la otra mitad recibió tratamiento con membrana de huevo en la herida superior. El reemplazo de la membrana, el desbridamiento de la herida y la obtención de imágenes se realizaron los días 5, 8 y 10. Luego continuaron las imágenes los días 12, 14, 16, 18 y 20 del experimento. La tasa de curación se midió en función del área de la herida durante los 20 días del experimento. Los resultados mostraron que las heridas con membrana de huevo de gallina tuvieron una tasa de curación significativamente más rápida en comparación con el control en las primeras etapas de curación entre los días 0 y 5. Este grupo curó un 21 por ciento más rápido durante esta fase temprana, en comparación con el grupo de control. Sin embargo, las tasas de curación de heridas no se distinguieron entre los días 5 y 20. Por lo tanto el vendaje de membrana de huevo de gallina mejora significativamente la cicatrización de heridas cutáneas, en las primeras etapas de la cicatrización de heridas. En el estudio de Li X y otros,3 realizado en 2019 se analizaron una serie de diferentes películas de mezcla de cáscara de huevo (ESM) y quitosano (CS) para el vendaje de heridas. Se evaluaron varias propiedades relacionadas con el cuidado de la herida, como la integridad de la película en solución, pH, proteína (BSA) y capacidad de absorción de líquido de la herida, así como la propiedad antibacteriana de las películas. Las películas de mezcla de ESM y CS fueron más estables que las películas CS después de 95 horas de incubación en solución. La integridad de las películas de mezcla mejoró significativamente a costa de una pequeña disminución insignificante en la capacidad de absorción de fluido de la herida. Además, las películas de mezcla proporcionaron un ambiente ácido (pH = 5,86) para la cicatrización de heridas. Las propiedades de hinchamiento de ESM contribuyeron significativamente al aumento de la capacidad de absorción de BSA de las películas de mezcla (de 46,57 miligramos/gramo de película CS a 61,07 miligramos/gramo de película de mezcla) y ayudaron a absorber más nutrientes para promover la proliferación y migración de fibroblastos. La adición de CS a ESM también mejoró significativamente la actividad antibacteriana de las películas. Los resultados indicaron que las películas de mezcla EMS/CS con 0,01 g de solución de ESM/mL CS mostraron el mayor potencial para ser utilizado como apósito para el cuidado de heridas tanto en humanos como en animales. Según un reciente informe de la Unión Europea,4 la membrana de la cáscara de los huevos de polluelos favorece la cicatrización de las heridas. Según este informe, la membrana de la cáscara de huevo es una alternativa rentable a los apósitos de colágeno. El compuesto de la membrana se purifica y posteriormente se muele, para formar un polvo que se incorpora al apósito. Es similar a un apósito hidrocoloide, flexible y transparente, que se pone directamente sobre la herida y se cubre con un apósito secundario a base de espumas poliméricas o fibras de algodón. Estos apósitos disminuyen el daño tisular, la inflamación, aceleran la curación al unirse a las metaloproteinasas de matriz, se incrementa la creación de tejido conjuntivo nuevo, así como de vasos sanguíneos. Puede usarse en heridas de cualquier tamaño y forma, incluyendo heridas secas, húmedas, llagas y úlceras. Tras examinar los resultados de los estudios de los últimos años se puede dilucidar el potencial de la membrana de cáscara de huevo para favorecer la curación de las heridas, reducir el daño cutáneo y la inflamación. Teniendo en cuenta estos resultados, el propósito de esta carta es fundamentalmente trasmitir estos novedosos conocimientos a los investigadores sanitarios que trabajan diariamente con este tipo de enfermedades cutáneas. De esta forma, podrán diseñar y ofrecer a sus pacientes los mejores apósitos, basados en las evidencias sanitarias más actualizadas. Es importante resaltar que aunque la evidencia revisada, parece indicar que se pueden esperar resultados positivos de este producto, la escasa cantidad de ensayos clínicos aleatorizados, el reducido número de estudios realizados en humanos y la escasez en la muestra, no permite actualmente realizar recomendaciones generalizadas. Es necesario por tanto, aumentar la cantidad de estudios dentro de este ámbito. De esta forma se podrá evaluar la eficacia y posibles complicaciones de este tratamiento a largo plazo, analizar el efecto sobre diversos tipos de heridas y examinar su posible efecto sinérgico con otras terapias, con otros apósitos o con otros productos(AU)


Subject(s)
Animals , Wound Healing , Chickens , Egg Shell , Biofilms , Research Report
5.
Acta toxicol. argent ; 28(1): 5-12, Apr. 2020. graf
Article in English | LILACS | ID: biblio-1248663

ABSTRACT

Abstract In the 1960s, organochlorine compounds were responsible for the decline of birds of prey populations such as Haliaee- tus leucocephalus and Falco peregrinus. Pesticides similar to DDT cause bioaccumulation in birds, affecting their eggshell com- position and compromising their development. Using system biology tools, the goal of this study was to better comprehend how organochlorines act on birds. We performed a literature review, using the STITCH 5.0 platform, searching for the terms DDT and TCDD. The sub-networks were amplified in 100 interactions in STRING 10.5 and joined by the Cytoscape 3.4.0 Merge software, using the experimental animal model Gallus gallus. Clusterization, gene ontology, and centrality were the parameters evaluated in the resulting network. The resulting network had 1,417 interactions and 137 nodes. The clusterization indicated four clusters and the gene ontology pointed to biological processes related to cell signaling and morphological development. The centrality analysis indicated ESR1 and HSP90AB1 as hub/bottleneck proteins involved in the estrogen pathway and calcium transport. Therefore, it is possible that HSP90 proteins have increased expression in birds contaminated with organochlorine pesticides, favoring ESRI-organochlorines interaction and disturbing the calcium availability related to the eggshell formation. The presence or absence of heat shock proteins, such as HSP90, influences several aspects of reproduction in many species. Therefore, the relationship between the HSP90 protein expression and thin-shell syndrome was identified for the first time in this in silico study.


Resumen En los años 60, los organoclorados fueron responsables del declive de aves de rapiña como Haliaeetus leucocephalus y Falco peregrinus. Pesticidas como el DDT, causan biomagnificación en las aves, afectando las cáscaras de los huevos y dañando su desarrollo. El objetivo de este trabajo fue, a través de herramientas de biología de sistemas, comprender cómo los organoclorados actúan en el organismo de las aves. A través de una revisión bibliográfica se incluyeron dos compuestos, DDT y TCDD. Estos fue ron prospectos en la plataforma STITCH 5.0. Las subredes encontradas fueron aumentadas en 100 interacciones en la plataforma STRING 10.5 y unidas por la herramienta Merge del programa Cytoscape 3.4.0, usando el modelo experimental Gallus gallus. En la red resultante se analizaron la clusterización, la ontología génica y la centralidad. La red resultante presentó 137 nudos y 1.417 interacciones. El análisis de clusterización indicó 4 clusters, siendo que el análisis y ontología génica apuntó procesos biológicos ligados a la señalización y al desarrollo morfológico. El estudio de centralidad apuntó a ESR1 y HSP90AB1 como los hubs-bottle- neck proteínas que estaban involucradas en la vía de recepción de estrógeno y en el transporte de calcio. De acuerdo con los resultados podemos inferir que las proteínas HSP90 tienen su expresión aumentada, en aves contaminadas con pesticidas organoclo rados, favoreciendo la interacción entre ESRI y DDT / TCDD. Con ello, la interacción ESRI y la hormona estrógeno se compromete perjudicando el transporte de calcio y consecuentemente la formación de la cáscara del huevo en aves expuestas. La expresión de proteínas de choque térmico ha sido asociada a varios aspectos de la reproducción en muchas especies, sin embargo, una asociación entre HSP90 y el síndrome de la cáscara fina del huevo fue identificada por primera vez en este experimento in silico.


Subject(s)
Animals , Raptors/abnormalities , Egg Shell/abnormalities , Insecticides, Organochlorine/adverse effects , Computer Simulation , Gene Ontology
6.
Article in English | LILACS, BBO | ID: biblio-1135552

ABSTRACT

Abstract Objective: To evaluate and compare the efficacy of eggshell derived calcium hydroxyapatite with Dycal® as indirect pulp capping material in primary molars. Material and Methods: A total of 32 carious primary molars from 22 children (6-10-years) were screened, of which 26 primary molars meeting inclusion criteria were selected and equally divided into two groups. At the first appointment, the infected dentin was excavated using a spoon excavator after treating the carious part with Carie-Care™ chemomechanical caries removal agent. After this, eggshell derived calcium hydroxyapatite in Group 1 and Dycal® in Group 2 were used as liners followed by restoration of the cavity with type IX glass ionomer cement. Clinical assessment for pain and radiographic assessment for measurement of the amount of reparative dentin thickness formation was performed at baseline, 8 weeks and 3 months. The data were subjected to statistical analysis by one-way ANOVA and Kruskal-Wallis tests. Results: Higher mean reparative dentin formation was found in eggshell derived calcium hydroxyapatite group than Dycal® group at the end of 8 weeks and 3 months and the difference was significant statistically (p<0.001). Conclusion: Eggshell derived calcium hydroxyapatite seems to be a suitable alternative to Dycal® (calcium hydroxide) that can be used as a liner for indirect pulp capping in primary molars.


Subject(s)
Humans , Male , Female , Child , Tooth, Deciduous , Durapatite/therapeutic use , Dental Pulp Capping , Dentin, Secondary/anatomy & histology , Molar , Radiography, Dental/instrumentation , Efficacy , Analysis of Variance , Statistics, Nonparametric , Egg Shell , Glass Ionomer Cements , India/epidemiology
7.
Arq. bras. med. vet. zootec. (Online) ; 71(5): 1623-1630, set.-out. 2019. tab
Article in Portuguese | VETINDEX, LILACS | ID: biblio-1038682

ABSTRACT

O experimento foi realizado no setor de avicultura/UFRRJ, utilizando 348 galinhas semipesadas (linhagem Dekalb Brown), com 52 semanas de idade, criadas sob dois sistemas de produção: cage-free e em gaiolas. Os dados obtidos pela análise físico-química e microbiológica dos ovos e a resistência óssea à quebra foram submetidos à análise de variância. No caso de ocorrerem efeitos dos diferentes sistemas de produção, foi aplicado o teste de Tukey a 5% de probabilidade para comparação das médias. A qualidade físico-química foi igualmente favorecida pelos dois sistemas de produção, indicando que as circunstâncias experimentais propiciaram condições adequadas para a formação de ovos de boa qualidade. O sistema de gaiola não desfavoreceu as características ósseas das galinhas, apontando que, em densidades adequadas, a gaiola pode não exercer um fator prejudicial para a qualidade óssea. O sistema de produção cage-free piorou a contaminação da casca, comprovando que ovos postos em ninhos são mais contaminados em comparação aos produzidos em gaiolas.(AU)


The experiment was carried out in the poultry sector / UFRRJ, using 348 semi-heavy hens (Dekalb Brown line), 52 weeks old, raised under two cage-free production systems and cages. The data obtained by the physical-chemical and microbiological analysis of the eggs and the bone resistance to the break were submitted to analysis of variance, in case of effects of the different production systems, the Tukey's test was applied at 5% of probability for comparison of the means. The physical-chemical quality was also favored by the two production systems, indicating that the experimental circumstances provided adequate conditions for the formation of good quality eggs. The cage system did not disfavor the bony characteristics of the hens, indicating that at suitable densities, the cage may not exert a detrimental factor to bone quality. The cage-free production system worsened shell contamination by proving that nesting eggs are more contaminated compared to those produced in cages.(AU)


Subject(s)
Animals , Poultry/growth & development , Chickens/growth & development , Egg Shell/growth & development , Eggs/analysis , Eggs/microbiology , Animal Husbandry , Animal Welfare
8.
Biomédica (Bogotá) ; 39(supl.1): 50-62, mayo 2019. tab, graf
Article in English | LILACS | ID: biblio-1011454

ABSTRACT

Abstract Introduction: Salmonella Enteritidis is a major cause of human salmonellosis in the world, with contaminated eggs and raw chicken meat as the main routes of infection. The main Salmonella spp. serovars circulating in laying hen farms, the surface of eggs, and in raw chicken carcasses have been identified in Ibagué, Colombia. However, it is unknown whether those serovars are responsible for human gastroenteritis. Objective: To evaluate the genetic relationship between gastroenteritis and Salmonella Enteritidis isolates from poultry and humans using multilocus sequence typing (MLST). Materials and methods: Salmonella spp. was isolated from clinical cases of gastroenteritis (n=110). Antibiotic susceptibility tests, followed by serotyping and MLST were conducted and S. Enteritidis was compared to those from laying hen farms and marketed eggs. Results: Ten isolates of Salmonella spp. were obtained from the stools of people with gastroenteritis. The prevalence of Salmonella spp. in human stools was 9.09%, and S. Enteritidis (n=4), S. Typhymurium (n=2), S. Newport (n=1), S. Uganda (n=1), S. Grupensis (n=1), and S. Braenderup (n=1) were the main serotypes. MLST indicated that a common S. Enteritidis sequence type (ST11) was present in all three sources and showed the same antibiotic resistance pattern. Conclusion: Salmonella Enteritidis ST11 constitutes a link between consumption and manipulation of contaminated eggs and human gastroenteritis in Ibagué. Additional studies would be required to establish if other Salmonella serovars isolated from raw chicken meat are also associated with human gastroenteritis.


Resumen Introducción. Salmonella Enteritidis es una de las mayores causas de salmonelosis en el mundo; los huevos contaminados y la carne de pollo cruda son sus principales fuentes de infección. En Ibagué, Colombia, se han identificado los principales serovares que circulan en granjas, superficies de huevos y canales de pollo, pero se desconoce si esos serovares son responsables de la gastroenteritis. Objetivo. Evaluar la relación genética entre los aislamientos de Salmonella Enteritidis de aves de corral y de humanos con la gastroenteritis mediante tipificación de multiloci de secuencias (Multilocus Sequence Typing, MLST). Materiales y métodos. Se aisló Salmonella spp. de casos clínicos de gastroenteritis (n=110). Se hizo la prueba de sensibilidad antibiótica, así como la serotipificación y la tipificación mediante MLST, y se comparó S. Enteritidis de humanos con la hallada en granjas de gallinas ponedoras y en huevo comercializado (n=6). Resultados. Se aislaron 10 cepas de Salmonella spp. a partir de heces de humanos con gastroenteritis. Se obtuvo una prevalencia de Salmonella spp. de 9,09%, y se identificaron los serotipos S. Enteritidis (n=4), S. Typhymurium (n=2), S. Newport (n=1), S. Grupensis (n=1), S. Uganda (n=1) y S. Braenderup presentes en pacientes con gastroenteritis. Mediante la MLST, se comprobó que un tipo de secuencia común (ST11) de S. Enteritidis estuvo presente en todas las tres fuentes y presentó el mismo patrón de resistencia antibiótica. Conclusión. Salmonella Enteritidis ST11 constituye un vínculo entre el consumo y la manipulación de huevos contaminados, y la gastroenteritis en humanos en Ibagué. Se requieren estudios complementarios para conocer si otros serovares de Salmonella aislados de carne de pollo cruda también se asocian con la gastroenteritis en humanos.


Subject(s)
Animals , Humans , Poultry Diseases/microbiology , Salmonella enteritidis/genetics , Salmonella Food Poisoning/microbiology , Salmonella Infections, Animal/microbiology , DNA, Bacterial/genetics , Gastroenteritis/microbiology , Phylogeny , Poultry , Poultry Diseases/epidemiology , Salmonella enteritidis/isolation & purification , Salmonella enteritidis/classification , Salmonella enteritidis/drug effects , Salmonella Food Poisoning/epidemiology , Salmonella Infections, Animal/epidemiology , Drug Resistance, Microbial , Base Sequence , Cross-Sectional Studies , Sequence Analysis, DNA , Colombia/epidemiology , Egg Shell/microbiology , Feces/microbiology , Multilocus Sequence Typing , Serogroup , Gastroenteritis/veterinary , Gastroenteritis/epidemiology
9.
Hig. aliment ; 33(288/289): 1153-1157, abr.-maio 2019. tab
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1482118

ABSTRACT

Objetivou-se verificar a qualidade de ovos brancos de acordo com os aspectos internos e externos comercializados em diferentes estabelecimentos do município de Paragominas - PA. Os ovos foram coletados de aleatoriamente em diferentes pontos comerciais. As análises consideradas no estudo, foi de: integridade e aparência da casca e peso do ovo, altura da câmara de ar, peso, altura e diâmetro da gema para o cálculo de índice da gema, altura e peso do albúmen e cor da gema. Os dados foram submetidos à análise de variância e ao teste de Tukey. Os ovos dos grandes supermercados apresentaram melhores índices de qualidade interna e os piores resultados quanto a peso, trincas visíveis e sujeiras. Os ovos de pequenos supermercados apresentaram o maior índice de trincas não visíveis e os de médio porte os menores índices de sujeiras e trincas.


Subject(s)
Animals , Egg Shell , Egg Yolk , Eggs/analysis , Eggs/statistics & numerical data , Weights and Measures , Poultry , Food Quality
10.
Hig. aliment ; 33(288/289): 2046-2050, abr.-maio 2019. tab
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1482459

ABSTRACT

Este trabalho objetivou avaliar a qualidade microbiológica de ovos caipiras comercializados na feira livre de São Joaquim na cidade de Salvador – BA. Foram realizadas análises para verificar a presença de Salmonella spp. e, também, a ocorrência e quantificação de bactérias do grupo Coliformes totais, Escherichia coli e Staphylococcus spp. em oito amostras, totalizando 96 ovos, analisados separadamente em casca e conteúdo. A presença de Salmonella spp. não foi verificada na casca de nenhuma das amostras, no entanto em 50% das cascas de ovos analisadas foi verificada a ocorrência de Coliformes totais com contagens entre <10(2) e incontáveis colônias UFC/g, a ocorrência de Escherichia coli foi confirmada em 12,5% das cascas com contagens de <10(2) a 3,0 x 103 UFC/g, já Staphylococcus spp ocorreram na casca de 75% amostras analisadas variando entre <10(2) e incontáveis colônias UFC/g. Não foi verificada a ocorrência dos microrganismos pesquisados no conteúdo dos ovos analisados. Estes resultados indicam a necessidade de melhoria nas práticas de manipulação que visem à redução da incidência desses microrganismos nas cascas dos ovos.


Subject(s)
Egg Shell/microbiology , Food Contamination/analysis , Eggs/microbiology , Food Microbiology , Food Quality
11.
Braz. oral res. (Online) ; 33: e016, 2019. tab, graf
Article in English | LILACS | ID: biblio-989478

ABSTRACT

Abstract To synthesize Nano eggshell-titanium-dioxide (EB@TiO2) biocomposite and to evaluate its effectiveness in occluding opened dentine tubules. EB@TiO2 was synthesized and characterized using X-ray diffraction (XRD), and Transmission Electron Microscope (TEM). Sixteen simulated bovine dentine discs were prepared and randomly assigned into four groups according to the following treatment (n = 4): Group 1: No treatment; Group 2: eggshell powder; Group 3: EB@TiO2; Group 4: Sensodyne. These were then agitated in a solution of 1g powder and 40mL water for 3hours. Thereafter, each dentine discs from the respective groups were post-treated for 5 min with 2wt% citric acid to test their acid resistant characteristics. Scanning Electron Microscope (SEM) was used to observe the effectiveness of occluded dentine pre-and post-treatment. The cytotoxicity of the synthesized EB@TiO2 was tested using NIH 3T3 assay. ANOVA was used to evaluate the mean values of the occluded area ratio and the data of MTS assay. This was followed by a multi-comparison test with Bonferroni correction (α = .05). The XRD confirmed that EB@TiO2 was successfully modified through ball-milling. The TEM revealed the presence of both spherical and irregular particle shape powders. The SEM result showed that EB@TiO2 could effectively occlude open dentine tubules. Equally, the result demonstrated that EB@TiO2 exhibited the highest acid resistant stability post-treatment. NIH 3T3 assay identified that EB@TiO2 had little effect on the NIH 3T3 cell line even at the highest concentration of 100µg/ml. This study suggests that the application of EB@TiO2 effectively occluded dentine tubules and the occlusion showed a high acid resistant stability.


Subject(s)
Animals , Cattle , Mice , Phosphates/pharmacology , Titanium/chemistry , Dentin Permeability/drug effects , Dentin Sensitivity/therapy , Egg Shell/chemistry , Nanocomposites/chemistry , Dentin Desensitizing Agents/pharmacology , Fluorides/pharmacology , Nitrates/pharmacology , Titanium/analysis , Titanium/pharmacology , Tooth Remineralization , Microscopy, Electron, Scanning , NIH 3T3 Cells , Drug Combinations , Egg Shell/ultrastructure , Nanocomposites/analysis , Nanocomposites/therapeutic use
12.
An. acad. bras. ciênc ; 90(1): 529-539, Mar. 2018. tab
Article in English | LILACS | ID: biblio-886899

ABSTRACT

ABSTRACT The effects of increasing levels of soybean acid oil in diets of quails on the quality of eggs were evaluated over a period of 56 days. A completely randomized design with five treatment levels of soybean acid oil (0, 2, 4, 6, and 8%) as a replacer for soybean oil and eight replicates of two quails each were used. A total of 240 fresh eggs were used for quality analysis, and 400 eggs were stored at 23ºC over a period of 0, 7, 14, 21, and 28 days for analysis of shelf-life. Polynomial regression and ANOVA with repeated measurements and Tukey´s tests were used. Soybean acid oil linearly increased the intensity of red (a* = -5.26 + 0.14 x, R2 = 0.89, P = 0.01) and yellow (b* = 42.32 + 0.44 x, R2 = 0.88 P = 0.01). All other variables of egg quality were not affected by the treatments, with the exception of sensory attributes, such as odor and aftertaste, which were more pronounced with soybean acid oil. In conclusion, soybean acid oil affects the intensity of yellow and red color of the yolk. Trained assessors detected differences in odor and aftertaste of eggs when soybean acid oil was included in the quail diet.


Subject(s)
Animals , Soybean Oil/chemistry , Coturnix/physiology , Eggs , Animal Feed/analysis , Animal Nutritional Physiological Phenomena/physiology , Reference Values , Time Factors , Soybean Oil/analysis , Reproducibility of Results , Analysis of Variance , Diet , Egg Shell/anatomy & histology
13.
Arq. bras. med. vet. zootec. (Online) ; 69(5): 1301-1310, set.-out. 2017. tab, ilus, graf
Article in English | LILACS, VETINDEX | ID: biblio-879221

ABSTRACT

This study evaluated the influence of the bird's age on the quality of the shell and percentage of bacterial penetration in commercial eggs. White-shelled commercial eggs were used, laid by light laying hens in their first laying cycle at 21, 39, 51, and 62 weeks of age. Shell quality evaluations comprised: egg weight, specific gravity, percentage and thickness of shell, number and size of pores. For evaluations regarding bacterial penetration, strains of several enterobacterias and one salmonella were used, all of which resistant to Nalidixic acid (100µg/ml). The method employed for evaluation of bacterial penetration was filling the eggs with growth medium. The data were subjected to variance analysis with 5% of probability using SAS (Education Analytical Software, 2013). Eveb though increase in the laying hen's age caused reduction of the quality of eggshells, it failed to affect the percentage of penetration of the bacterial samples evaluated.(AU)


O presente estudo avaliou a influência da idade da ave sobre a qualidade da casca e na porcentagem de penetração bacteriana em ovos comerciais. Foram utilizados ovos brancos comerciais provenientes de poedeiras leves em primeiro ciclo de postura com 21, 39, 51 e 62 semanas de idade. As avaliações de qualidade da casca realizadas foram: peso do ovo, gravidade específica, porcentagem e espessura da casca, número e tamanho dos poros. Para as avaliações da penetração bacteriana, foram utilizadas cepas de diversas enterobactérias e uma salmonela, sendo todas resistentes ao ácido nalidíxico (100µg/mL). O método utilizado para a avaliação da penetração bacteriana foi por meio do preenchimento dos ovos com meio de cultura. Os dados foram submetidos à análise de variância com 5% de probabilidade utilizando-se o programa SAS - Statistical Analisys System (Education Analytical Software, 2013). O aumento da idade da poedeira promoveu a redução da qualidade da casca dos ovos, porém não foi capaz de influenciar a porcentagem de penetração das amostras bacterianas avaliadas.(AU)


Subject(s)
Animals , Female , Age Factors , Chickens , Egg Shell/microbiology , Eggs/analysis , Enterobacteriaceae
14.
The Korean Journal of Parasitology ; : 313-317, 2017.
Article in English | WPRIM | ID: wpr-168666

ABSTRACT

Paragonimiasis is a parasitic disease caused by Paragnonimus species. The primary site of infection is the lung, and extrapulmonary involvement is also reported. When infected with Paragonimus westermani, which is the dominant species in Korea, the central nervous system is frequently involved along with the liver, intestine, peritoneal cavity, retroperitoneum, and abdominal wall. Ectopic paragonimiasis raises diagnostic challenge since it is uncommon and may be confused with malignancy or other inflammatory diseases. Here, we report an ectopic paragonimiasis case initially presented with recurrent abdominal pain. The patient developed abdominal pain 3 times for the previous 3 years and the computed tomography (CT) of the abdomen revealed fluid collection with wall enhancement. Recurrent diverticulitis was initially suspected and part of the ascending colon was resected. However, the specimen showed intact colon wall without evidence of diverticulitis and multiple parasite eggs and granulomas were found instead. The size of about 70 μm, the presence of an operculum and relatively thick egg shell suggested eggs of Paragonimus species. With appropriate exposure history and a positive antibody test, the definitive diagnosis was made as peritoneal paragonimiasis.


Subject(s)
Animals , Humans , Abdomen , Abdominal Abscess , Abdominal Pain , Abdominal Wall , Abscess , Central Nervous System , Colon , Colon, Ascending , Diagnosis , Diverticulitis , Egg Shell , Eggs , Granuloma , Intestines , Korea , Liver , Lung , Ovum , Paragonimiasis , Paragonimus , Paragonimus westermani , Parasites , Parasitic Diseases , Peritoneal Cavity
15.
An. acad. bras. ciênc ; 89(3,supl): 2479-2484, 2017. tab
Article in English | LILACS | ID: biblio-886791

ABSTRACT

ABSTRACT The aim of this study was to evaluate the effect of Syzygium cumini leaves (SCL) in laying hens diets on productive performance, egg quality and lipid oxidation of yolk. A total of 108 Hysex White laying hens were distributed in a completely randomized design with three treatments and six replicates of six birds each. The treatments consisted of SCL inclusion at dietary levels of 0, 5 and 10 g/kg. There was no significant effect of SCL inclusion on feed intake, laying percentage, weight and egg mass, feed conversion ratio, Haugh units, specific gravity, percentage of yolk, albumen and egg shells and shell thickness. However, the inclusion of SCL significantly influenced the yolk color and yolk lipid oxidation measured by TBARS values. Yolk color increased and TBARS values ​​decreased with the inclusion of SCL. The inclusion of SCL in laying hens diets improves pigmentation and lipid stability of yolk.


Subject(s)
Animals , Female , Oviposition/drug effects , Plant Extracts/analysis , Chickens/physiology , Plant Leaves , Dietary Supplements/analysis , Animal Feed/analysis , Brazil , Syzygium , Diet , Egg Shell , Egg Yolk , Animal Nutritional Physiological Phenomena
16.
Arq. bras. med. vet. zootec ; 68(6): 1690-1696, nov.-dez. 2016. tab
Article in English | LILACS, VETINDEX | ID: biblio-827938

ABSTRACT

The study aimed to evaluate the effect of different levels of chlorine in drinking water of laying hens on zootechnical performance, eggs shell quality, hemogasometry levels and calcium content in tibia. 144 Hy-Line laying hens, 61 weeks old, were used distributed in 24 metabolism cages. They were subjected to water diets, for a period of 28 days, using sodium hypochlorite as a chlorine source in order to obtain the following concentrations: 5ppm (control), 20ppm, 50ppm, and 100ppm. Their performance was evaluated through water consumption, feed intake, egg production and weight, egg mass, feed conversion. Shell quality was measured by specific gravity. At the end of the experiment, arterial blood was collected for blood gas level assessment and a poultry of each replicate was sacrificed to obtain tibia and calcium content measurement. There was a water consumption reduction from 20ppm of chlorine and feed intake reduction in poultry receiving water with 100ppm of chlorine. The regression analysis showed that the higher the level of chlorine in water, the higher the reduction in consumption. There were no differences in egg production and weight, egg mass, feed conversion, specific gravity, tibia calcium content, and hemogasometry levels (hydrogenionic potential, carbon dioxide partial pressure, oxygen partial pressure, sodium, potassium, chloride, bicarbonate, carbon dioxide total concentration, anion gap and oxygen saturation). The use of levels above 5ppm of chlorine is not recommended in the water of lightweight laying hens.(AU)


Objetivou-se avaliar o efeito de diferentes níveis de cloro na água de dessedentação de poedeiras sobre o desempenho zootécnico, a qualidade da casca dos ovos, os índices hemogasométricos e o teor de cálcio na tíbia. Foram utilizadas 144 poedeiras leves Hy Line, de 61 semanas de idade, distribuídas em 24 gaiolas de metabolismo. As aves foram submetidas, durante um período de 28 dias, a dietas hídricas utilizando-se hipoclorito de sódio como fonte de cloro para compor as seguintes concentrações: 5ppm (controle), 20ppm, 50ppm e 100ppm. O desempenho foi avaliado mediante o consumo de água e ração, a produção e o peso dos ovos, a massa de ovos e a conversão alimentar. A qualidade da casca foi aferida por meio da gravidade específica. Ao final do experimento, coletou-se sangue arterial para avaliação dos índices hemogasométricos e sacrificou-se uma ave de cada repetição para coleta de tíbias e aferição do teor de cálcio. Verificou-se redução do consumo de água a partir de 20ppm de cloro e no consumo de ração em aves que receberam água com 100ppm de cloro. A análise de regressão demonstrou que quanto maior for o nível de cloro na água, maior será a redução no consumo. Não foram observadas diferenças na produção e no peso dos ovos, na massa de ovos, na conversão alimentar, na gravidade específica dos ovos, no teor de cálcio nas tíbias e nos índices hemogasométricos (potencial hidrogeniônico, pressão parcial de gás carbônico, pressão parcial de oxigênio, sódio, potássio, cloro, bicarbonato, concentração total de dióxido de carbono, ânion gap e saturação de oxigênio). Não se recomenda a utilização de níveis superiores a 5ppm de cloro na água de dessedentação de poedeiras leves.(AU)


Subject(s)
Animals , Chickens , Chlorine/administration & dosage , Egg Shell , Eggs/analysis , Blood Gas Analysis/veterinary , Drinking
17.
Arq. bras. med. vet. zootec ; 67(1): 249-254, 2/2015. tab
Article in Portuguese | LILACS | ID: lil-741098

ABSTRACT

Foram utilizadas 350 aves da linhagem comercial Bovans White, com o objetivo de avaliar a associação de carboidrases e fitase em dietas valorizadas sobre o desempenho e qualidade dos ovos de poedeiras leves. O delineamento experimental foi inteiramente ao acaso, com 5 tratamentos e 7 repetições. Os tratamentos foram: T1- Controle positivo sem adição de enzimas e sem valorização dos nutrientes; T2- Controle negativo (CN) 1, valorizando em 1,5% e 6% a EMA (kcal/kg), respectivamente para o milho e o farelo de soja, e em 2% a proteína bruta (PB) e os aminoácidos digestíveis limitantes mais a matriz completa para a enzima fitase; T3- CN 2, valorizando em 1,5% e 6% a EMA (kcal/kg), respectivamente para o milho e o farelo de soja, e em 2% a proteína bruta (PB) e os aminoácidos digestíveis limitantes mais a matriz incompleta para a enzima fitase; T4- suplementado com 100g ton-1 de carboidrase e 30g ton-1 de fitase; T5- suplementado com 100g ton-1 de carboidrase e 30g ton-1 de fitase. Os tratamentos controle positivo, T2, T3, T4 e T5, com ou sem suplementação enzimática, garantiram o desempenho no que diz respeito ao consumo de ração, produção, peso e perdas de ovos e qualidade da casca.


A total of 350 commercial Bovans White laying hens were used to evaluate the association of carbohydrases and phytase in enriched diets and its effects on performance and egg quality of laying hens. The experiment used a randomized design with five treatments and seven replicates. The treatments were: 1. Positive control without added enzymes and without nutrient enrichment, 2. Negative control (NC) 1 with 1.5% and 6% AME (kcal/kg) enrichment for corn and soybean meal respectively, 2% crude protein (CP) enrichment, and digestible limiting digestible amino acids plus the full matrix for the phytase enzyme; 3. NC 2 with 1.5% and 6% AME (kcal/kg) enrichment, respectively, for corn and soybean meal and 2% crude protein (CP) enrichment, and digestible limiting amino acids plus the sparse matrix for the phytase enzyme, 4. NC 1 supplemented with 100 g ton-1 carbohydrase and 30g ton-1 phytase, 5. NC 2 supplemented with 100 g ton-1 carbohydrase and 30g ton-1 phytase. According to the results, the positive control treatments, NC1 and NC2, with or without enzyme supplementation, showed guaranteed performance for feed intake, egg yield, weight, egg loss and shell quality.


Subject(s)
Animals , Egg Shell/abnormalities , Animal Feed/analysis , Animal Feed/standards
18.
Acta odontol. latinoam ; 27(1): 3-8, July 2014. tab, graf
Article in English | LILACS | ID: lil-761841

ABSTRACT

El objetivo de este trabajo fue describir el efecto de la composición de una sustancia remineralizante (SRM) y de la presión osmótica sobre el color dental mediante espectrofotometría. Se tomaron 104 premolares y molares humanos repartidos aleatoriamente en 2 grupos, cada uno de 52 especímenes. El grupo 1 se trató con la sustancia remineralizante SRM 55 (agente remineralizante 1) mezcla de 50 por ciento - 50 por ciento de mineral de grano fino y otro mineral de grano grueso y el grupo 2 se trató con la sustancia remineralizante SRM 91(agente remineralizante 2) contienen los mismos minerales en proporción 90 por ciento - 10 por ciento. A su vez cada grupo se dividió en 2 subgrupos, cada uno de 26 especímenes que se almacenaron así: Un subgrupo en saliva sintética con presión osmótica isotónica (PI) y el otro con presión osmótica hipotónica (PH). Se tomaron registros iniciales y finales con el espectrofotómetro Vita Easy Shade®. Con las lecturas se calcularon losparámetros de color (L*; a*; b*) y los índices de blanqueamiento (WIC; WIO; W). Los cambios de color (ΔL; ΔA; ΔB; yΔE) y los índices de blanqueamiento se compararon y se trataron todos mediante un análisis descriptivo. Las variables ΔA, ΔL, ΔB, ΔE e índice de blanqueamiento W se trataron con ANOVA y los índices WIC y WIO con un análisis de varianza no paramétrico Kruskal Wallis. Los resultados indican que la combinación A2 (SRM 91 y PI) afectó las variables ΔB y ΔE. La combinación B1 (SRM 55 Y PH) afectó las variables ΔA, ΔB y el índice de blanqueamiento WIO. Solamente SRM 91afectó la variable ΔL. La presión osmótica de la saliva y la sustancia remineralizante afectan el color del esmalte dental...


Subject(s)
Humans , Male , Adolescent , Adult , Animals , Female , Young Adult , Dental Enamel , Biocompatible Materials/pharmacology , Tooth Remineralization/methods , Color , Egg Shell/chemistry , Spectrophotometry/methods , Tissue Extracts/pharmacology , Phosphates/pharmacology , Osmotic Pressure , Dental Prophylaxis/instrumentation , Saliva, Artificial , Hypotonic Solutions/chemistry , Isotonic Solutions/chemistry
19.
Rev. argent. microbiol ; 46(2): 122-125, jun. 2014.
Article in Spanish | LILACS | ID: biblio-1016607

ABSTRACT

En Argentina, Escherichia coli enteropatogénico (EPEC) es uno de los agentes más prevalentes aislados de niños con diarrea. Debido a que la contaminación con este patotipo en productos de pollo podría ocurrir durante el proceso de faena, nos planteamos como objetivo aislar y caracterizar EPEC de muestras de animales vivos (cloacas), carcasas evisceradas sin lavar, carcasas lavadas y agua del tanque de enfriamiento. Se caracterizaron 29 aislamientos de EPEC que presentaron una amplia variedad de serotipos, algunos de los cuales (O2:H40, O8:H19 y O108:H9) han sido informados en otras especies animales. También se encontró el serotipo O45:H8, aislado con anterioridad de niños con diarrea. Se detectaron aislamientos de los serotipos O2:H40, O108:H9 y O123:H32 en distintas etapas del proceso de faena, lo que sugiere que el procesamiento no se realiza en forma adecuada. Se torna necesario reforzar las medidas de control e higiene en las distintas etapas del proceso para disminuir la contaminación microbiana


In Argentina, EPEC is one of the most prevalent agents isolated from children with diarrhea. Because contamination with this pathotype could occur during slaughter, the aim of this study was to isolate and characterize EPEC strains obtained from live animals (cloacae), eviscerated carcasses, washed carcasses and water from chillers. Twenty nine isolates of atypical EPEC were characterized. These isolates presented a wide variety of serotypes, some of which (O2:H40, O8:H19 and O108:H9) had been reported in other animal species. Serotype O45:H8, previously isolated from children with diarrhea was also found. Isolates of serotypes O2:H40, O108:H9 and O123:H32 were detected at different stages of the slaughtering process, suggesting that the process is not adequately performed. This latter fact highlights the importance of reinforcing control and hygienic measures at different stages of the chicken slaughtering process in order to reduce microbial contamination


Subject(s)
Animals , Chickens/microbiology , Enteropathogenic Escherichia coli/isolation & purification , Colimetry/analysis , Cloaca/microbiology , Egg Shell/microbiology , Escherichia coli Infections/prevention & control , Enteropathogenic Escherichia coli/classification
20.
Journal of Veterinary Science ; : 579-582, 2014.
Article in English | WPRIM | ID: wpr-120174

ABSTRACT

Eggs exhibiting eggshell apex abnormalities (EAA) were evaluated for changes in shell characteristics such as strength, thickness, and ultrastructure. Mycoplasma synoviae (MS) infection was confirmed by serological assay along with isolation of MS from the trachea and oviduct. Changes in eggshell quality were shown to be statistically significant (p < 0.01). We also identified ultrastructural changes in the mammillary knob layer by Scanning Electron Microscopy. While eggs may seem to be structurally sound, ultrastructural evaluation showed that affected eggs do not regain their former quality. In our knowledge, this is the first report describing the occurrence of EAA in Korea.


Subject(s)
Animals , Chickens , Egg Shell/microbiology , Microscopy, Electron, Scanning/veterinary , Mycoplasma Infections/microbiology , Mycoplasma synoviae/physiology , Poultry Diseases/microbiology , Republic of Korea
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