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Rev. cuba. invest. bioméd ; 40(1): e599, ene.-mar. 2021. tab
Article in Spanish | LILACS, CUMED | ID: biblio-1289439


Introducción: Las infecciones de tracto urinario se encuentran entre las infecciones de mayor prevalencia en la parte clínica. Son un problema de salud global y se pueden presentar con o sin síntomas. Los agentes bacterianos aislados en mayor frecuencia son Escherichia coli, Klebsiella spp y Proteus spp. Objetivo: Caracterizar las infecciones de tracto urinario producidas por enterobacterias productoras de betalactamasas de espectro extendido en pacientes hospitalizados, Lima 2016-2018. Métodos: Se realizó un estudio descriptivo en 2 instituciones prestadoras de salud, en Lima, Perú, durante el periodo 2016-2018, a partir de los aislamientos de patógenos blee asociados a infecciones de tracto urinario. Se tuvieron en cuenta variables sociodemográficas, enfermedades asociadas, agentes aislados, tratamiento y respuesta clínica. Resultados: Se obtuvo un registro de 117 pacientes, con edad promedio de 58,18 ± 11,8 años; 65,0 por ciento fueron mujeres y 89,74 por ciento provenían del área urbana de Lima. Las enfermedades asociadas más frecuentes fueron diabetes (39,3 por ciento) y enfermedad renal moderada o grave (12,8 por ciento), con índice de Charlson medio de 2,70 ± 1,21. Los agentes aislados más comunes fueron Escherichia coli (92,3 por ciento), Klebsiella spp (6,0 por ciento) y Proteus spp (1,7 por ciento). Los tratamientos empíricos usados fueron ampicilina/sulbactam (18,9 por ciento), ciprofloxacino (49,6 por ciento) y nitrofurantoína (16,7 por ciento). El 49,2 por ciento de los pacientes recibió tratamiento dirigido, 22,8 por ciento ertapenem y 13,9 por ciento piperacilina/tazobactam. Conclusiones: Las personas con diabetes y enfermedad renal son un grupo vulnerable a las infecciones de tracto urinario. El agente causal aislado en mayor frecuencia fue Escherichia coli blee+. Los tratamientos de inicio luego de la identificación clínica de la infección urinaria fueron ciprofloxacino y cefalosporinas. Una vez obtenidos los resultados microbiológicos se modificó el tratamiento antibiótico a carbapenémicos y penicilinas. La revaloración de los antibióticos usados en pacientes con enfermedades asociadas es importante para el éxito del tratamiento(AU)

Introduction: Urinary tract infections are among the most prevalent infections in clinical practice. They are a global health problem and may present with or without symptoms. The bacterial agents most commonly isolated are Escherichia coli, Klebsiella spp. and Proteus spp. Objective: Characterize urinary tract infections caused by extended-spectrum betalactamase producing enterobacteria in hospitalized patients from Lima in the period 2016-2018. Methods: A descriptive study was conducted at two health institutions from Lima, Peru, in the period 2016-2018, based on isolation of ESBL pathogens associated to urinary tract infections. Attention was paid to sociodemographic variables, associated conditions, agents isolated, treatment and clinical response. Results: A sample was selected of 117 patients; mean age was 58.18 ± 11.8 years; 65.0 percent were women and 89.74 percent came from the urban area of Lima. The most common associated conditions were diabetes (39.3 percent) and moderate or serious kidney disease (12.8 percent), with a mean Charlson index of 2.70 ± 1.21. The most common isolated agents were Escherichia coli (92.3 percent), Klebsiella spp. (6.0 percent and Proteus spp. (1.7 percent). The empirical treatments used were ampicillin/sulbactam (18.9 percent), ciprofloxacin (49.6 percent) and nitrofurantoin (16.7 percent). 49.2 percent of the patients received targeted treatment, 22.8 percent ertapenem and 13.9 percent piperacillin/tazobactam. Conclusions: People with diabetes and kidney disease are vulnerable to urinary tract infections. The causative agent most commonly isolated was ESBL Escherichia coli. The initial treatments indicated after clinical identification of urinary infection were ciprofloxacin and cephalosporins. When microbiological results were obtained, antibiotic therapy was changed to carbapenems and penicillins. Reassessment of the antibiotics used in patients with associated conditions is important for the success of the treatment(AU)

Humans , Male , Female , Urinary Tract , Carbapenems , Kidney Diseases/drug therapy , Anti-Bacterial Agents
Braz. arch. biol. technol ; 63: e20200131, 2020. tab, graf
Article in English | LILACS | ID: biblio-1132247


Abstract Gallic acid (GA), as a strong antioxidant, was selected in this study to investigate its possible nephroprotective effects against gentamicin (GM)-induced nephrotoxicity. Twenty-four rats were separated into three groups (n=8): group 1 (control group) received saline (0.5 mL/day), group 2 (GM group) received GM (100 mg/kg/day), and group 3 (treated group) received GM (100 mg/kg/day) and GA (100mg/kg/day). All treatments were performed intraperitoneally for 12 days. After 12 days, the rats were euthanized, and kidneys were removed immediately. For serum preparation, blood samples were collected before killing. Kidney paraffin sections were prepared from one of the kidneys and stained by the periodic acid-Schiff process. GA significantly decreased GM-induced renal histopathological injuries, including tubular necrosis, tubular cast, and leucocyte infiltration compared with the GM group. Additionally, GA significantly improved proteinuria, serum levels of urea and creatinine, and serum activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) compared with nephrotoxic animals. Furthermore, GA caused a significant improvement in the levels of cholesterol (Chol), low-density lipoprotein (LDL), high-density lipoprotein (HDL), and cardiac risk ratios 1 and 2 in comparison with nephrotoxic animals. GA administration was observed to significantly improve the levels of lipid peroxidation, nitric oxide (NO), and glutathione (GSH) compared with the GM group. Finally, the activities and gene expression levels of catalase (CAT) and glutathione peroxidase (GPX) significantly increased following GA administration compared with the GM group. Our results indicated that GA has potential protective effects against GM nephrotoxicity by reducing oxidative stress in rats.

Animals , Male , Rats , Gentamicins/adverse effects , Oxidative Stress/drug effects , Gallic Acid/therapeutic use , Kidney Diseases/drug therapy , Anti-Bacterial Agents/adverse effects , Antioxidants/therapeutic use , Biomarkers , Cholesterol , Rats, Wistar , Disease Models, Animal , Gallic Acid/chemistry , Kidney Diseases/chemically induced , Kidney Diseases/pathology , Lipoproteins, HDL , Lipoproteins, LDL
Article in Chinese | WPRIM | ID: wpr-879931


OBJECTIVE@#To investigate the effect of Chinese medicine Wubi Shanyao pills on sexual function of kidney-yang-deficiency mice induced by hydrocortisone.@*METHODS@#Male Kunming mice were injected with hydrocortisone for 10 days to prepare the kidney-yang-deficiency model, and administrated with Wubi Shanyao pills (0.91, 1.82, 2.73 g/kg) for 9 weeks. The general behaviors of mice (autonomous activity, grasping power) were observed; sexual behaviors (capture, straddle, ejaculation frequency and incubation period) of mice were detected by mating experiment. The serum levels of cortisol, adrenocorticotropic hormone (ACTH), luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol (E@*RESULTS@#Wubi Shanyao pills increased the number of independent activities, grasping power, capture frequency of model mice and shortened the capture latency (all @*CONCLUSIONS@#Wubi Shanyao pills can improve the sexual function of mice with kidney-yang-deficiency induced by hydrocortisone, which may be related to regulating the hypothalamus-pituitary-adrenal axis (HPA axis), promoting the proliferation of testicular cells, and inhibiting cell apoptosis.

Animals , Follicle Stimulating Hormone/blood , Hydrocortisone , Hypothalamo-Hypophyseal System , Kidney/drug effects , Kidney Diseases/drug therapy , Male , Mice , Pituitary-Adrenal System/drug effects , Random Allocation , Sexual Behavior, Animal/drug effects , Yang Deficiency/drug therapy
Actual. osteol ; 15(3): 180-191, Sept-Dic. 2019. ilus
Article in English | LILACS | ID: biblio-1104226


Bone metabolism disorders are characterized by an imbalance of bone resorption and formation in the bone remodeling process. Glucocorticoids that are used to treat kidney diseases exacerbate these disorders. P-selectin and galectin-3 are molecules involved in the sclerotic process in kidney, whereas bone resorption is regulated by the interaction between the nuclear factor activator kappa b receptor (RANK), its ligand (RANKL) and the RANKL decoy receptor osteoprotegerin (OPG). The aim of this study was to investigate the cellular and molecular mechanisms of disruption of bone remodeling regulation processes, reflected by intercellular mediators (RANKL, OPG, P-selectin and galectin-3) in chronic kidney disease experimental model treated with glucocorticoids. Rats were divided into four groups of 10 animals each. The first group, the control group, included intact animals. The second group consisted of rats with impaired bone remodeling resulting from chronic kidney disease (experimental group (CKD). The third group was a group of animals with impaired bone remodeling due to exposure to glucocorticoids (experimental group (GCs)). The fourth group consisted of rats with impaired bone remodeling in chronic kidney disease, followed by exposure to glucocorticoids (experimental group (CKD + GCs)). The effects of CKD and glucocorticoid were evaluated biochemically, histologically and by measuring bone density. An enzymelinked immunoassay was used to measure intercellular mediator levels in the serum. The bone density in the experimental groups was reduced compared to the control group. RANKL levels in animals of three experimental groups were higher than in intact animals. Serum levels of OPG were higher in CKD and GCs groups than in intact animals. At the same time, in the animals' blood serum of the CKD + GCs group, the levels of OPG were lower, than those in animals from the control group. The levels of galectin-3 in the serum of the experimental groups GCs and CKD + GCs were lower than in intact animals. The serum levels of galectin-3 in animals of the CKD group were higher than those in animals from the control group. The levels of P-selectin were lower in the serum of the GCs group than in intact animals. At the same time, the levels of P-selectin were higher in the CKD and CKD + GCs groups, than those in animals from the control group. In conclusion, the study of the complex system of bone remodeling regulation, which includes many factors and their interactions, may lead to the development of new methods for treating patients with chronic kidney disease in order to prevent osteoporosis in the future. (AU)

Las enfermedades metabólicas óseas se caracterizan por un desequilibrio en el proceso de remodelación ósea en los que participan mediadores tales como receptor del activador del factor nuclear- kappa- b (RANK), su ligando (RANKL) y la osteoprotegerina (OPG). Los glucocorticoides, recuentemente empleados en el tratamiento de la enfermedad renal crónica, exacerban este desequilibrio. En la enfermedad esclerótica renal, las moléculas de adhesión celular P-selectina and galectina-3 tienen un rol fundamental. El objetivo de esta trabajo fue estudiar las alteraciones en los mediadores de la remodelación ósea (RANKL, OPG, P-selectina and galectina-3) en un modelo de enfermedad renal crónica con tratamiento glucocorticoideo. Ratas Wistar hembras fueron divididos en 4 grupos: control (C); enfermedad renal crónica con afección de la remodelación ósea (ERC); animales con afección de la remodelación ósea expuestos a glucocorticoides (GC); enfermedad renal crónica con afección de la remodelación ósea tratados con glucocorticoides (ERC+GC). Los efectos de la ERC y los GC fueron evaluados bioquímicamente, histológicamente y por medición de la densidad ósea. RANKL, OPG, Pselectina and galectina-3 se cuantificaron en muestras de sangre venosa empleando enzimoinmuno análisis. En los 3 grupos experimentales la densidad ósea se evidenció reducida y los niveles séricos de RANKL elevados respecto al grupo control. Los niveles de OPG en los grupos ERC y GC fueron superiores mientras que en el grupo ERC+GC menores respecto a los animales controles. Galectina 3 plasmática en GC y ERC+GC se encontró reducida y aumentada en los animales ERC, en comparación con los animales controles. La concentración sérica de P-selectina sérica fue mayor en los grupos ERC y ERC+GC, y menor en los animales GC respecto a los niveles plasmáticos de los animales intactos. El avance del conocimiento sobre la regulación de la remodelación ósea a través de la interacción de mediadores sistémicos, en un futuro, puede conducir al desarrollo de nuevas estrategias terapéuticas para la prevención de la osteoporosis en pacientes con enfermedad renal crónica. (AU)

Animals , Rats , Chronic Kidney Disease-Mineral and Bone Disorder/chemically induced , Bone Remodeling/drug effects , Kidney Diseases/physiopathology , Osteoporosis/prevention & control , Bone Diseases, Metabolic/diagnosis , Dexamethasone/administration & dosage , Bone Density/drug effects , Chloroform/therapeutic use , Rats, Wistar , P-Selectin/drug effects , P-Selectin/blood , Galectin 3/drug effects , Galectin 3/blood , RANK Ligand/drug effects , RANK Ligand/blood , Osteoprotegerin/drug effects , Osteoprotegerin/blood , Glucocorticoids/adverse effects , Glycerol/administration & dosage , Kidney Diseases/drug therapy
Int. j. morphol ; 37(2): 438-447, June 2019. tab, graf
Article in English | LILACS | ID: biblio-1002240


Obesity is a modifiable risk factor for the development and progression of kidney disease. Obesity may harm kidneys in individuals without hypertension, diabetes, or pre-existing renal disease. Ginger, Zingiber officinale, has many beneficial pharmaceutical benefits. This study aimed to evaluate the Zingiber officinale protective effect against obesity complications which induced by high fat diet and caused renal dysfunctions. The study period was two months, and the experimental animals' groups were four, 80 Wistar rats were appropriated similarly 20 animals/group: control group; ginger extract group (GE); high-fat diet (HFD); and GE+HFD group. Body and fat weight, creatinine, leptin, TNF-α, total antioxidants, renal histopathological and ultrastructure were investigated. Rats in group of HFD showed a significant increase (P<0.05) in the body and fat weights, creatinine, leptin and TNF-α, and significant decrease (P<0.05) in total antioxidants (TAS). Ginger administration significantly showed the protective restoring the altered parameters. Furthermore, rats co-treated with ginger extract improved the histopathological and ultrastructural renal injury induced by obesity. The study concluded that the ginger extract used could suppress and decrease the renal damage induced by high-fat diet as it possesses potential medicinal values.

La obesidad es un factor de riesgo modificable para el desarrollo y la progresión de la enfermedad renal. La obesidad puede dañar los riñones en personas sin hipertensión, diabetes o enfermedad renal preexistente. El jengibre, Zingiber officinale, tiene muchos beneficios farmacéuticos. Este estudio tuvo como objetivo evaluar el efecto protector de Zingiber officinale en las complicaciones de la obesidad inducida por una dieta alta en grasas y las enfermedad renal. El período de estudio fue de dos meses, y los grupos de animales experimentales fueron cuatro, se asignaron 80 ratas Wistar de manera similar, 20 animales por grupo: grupo de control; grupo de extracto de jengibre (GE); dieta alta en grasas (DAG); y el grupo GE + DAG. Se evaluó el peso corporal y la grasa, creatinina, leptina, TNF-α, antioxidantes totales, histopatología renal y ultraestructura. Las ratas en el grupo de DAG mostraron un aumento significativo (P<0,05) en el peso corporal y de grasa, creatinina, leptina y TNF-a, y una disminución significativa (P<0,05) en los antioxidantes totales. La administración de jengibre mostró una protección significativa restaurando los parámetros alterados. Además, las ratas tratadas conjuntamente con extracto de jengibre mejoraron la lesión renal histopatológica y ultraestructural inducida por la obesidad. El estudio concluyó que el extracto de jengibre podría suprimir y disminuir el daño renal inducido por la dieta alta en grasas, ya que posee potenciales valores medicinales.

Animals , Rats , Plant Extracts/pharmacology , Ginger/chemistry , Diet, High-Fat/adverse effects , Kidney Diseases/drug therapy , Obesity/complications , Body Weight , Tumor Necrosis Factor-alpha/analysis , Rats, Sprague-Dawley , Creatinine/analysis , Leptin/analysis , Microscopy, Electron, Transmission , Kidney/pathology , Kidney Diseases/pathology
Säo Paulo med. j ; 137(1): 39-44, Jan.-Feb. 2019. tab
Article in English | LILACS | ID: biblio-1004743


ABSTRACT BACKGROUND: Up to 5% of familial Mediterranean fever (FMF) cases are unresponsive to colchicine, through resistance, side effects and toxicity. Anakinra is an alternative treatment for FMF patients whose disease remains uncontrolled with colchicine. We aimed to evaluate anti-interleukin-1 treatment regarding clinical findings, laboratory parameters and quality of life (QoL) among FMF patients presenting resistance and toxicity towards colchicine. DESIGN AND SETTING: Descriptive observational study at the rheumatology clinic, Adnan Menderes University Medical School, Aydın, Turkey. METHODS: Among the patients included, age, sex, MEFV genotypes, acute-phase reactants, hepatic/renal function tests, average colchicine dose, disease duration, attack frequency, attack duration, disease severity, proteinuria, amyloidosis and QoL were evaluated. Colchicine resistance was defined as > 6 typical episodes/year or > 3 per 4-6 months. Kolmogorov-Smirnov, Friedman and two-way analysis of variance tests were used for statistical analyses. RESULTS: Between 2015 and 2017, 14 FMF patients receiving anakinra were enrolled. The mean colchicine dose was 1.7 ± 0.3 mg/day before use of anakinra. Ten patients were attack-free after treatment, while three showed reductions of at least 50% in attack frequency, attack duration and disease severity. Proteinuria levels in all patients with renal amyloidosis decreased after treatment. QoL among patients with renal amyloidosis differed significantly from QoL among non-amyloidosis patients. Mean visual analogue scale scores significantly improved in both groups after use of anakinra. CONCLUSIONS: Use of anakinra reduced attack frequency and proteinuria and acute-phase reactant levels, and improved QoL, with only a few uncomplicated side effects among colchicine-resistant or intolerant FMF patients. Injection-site reactions of severity insufficient to require discontinuation of treatment were seen.

Humans , Male , Female , Adult , Middle Aged , Familial Mediterranean Fever/drug therapy , Quality of Life , Drug Resistance/drug effects , Colchicine/therapeutic use , Interleukin-1/antagonists & inhibitors , Antirheumatic Agents/therapeutic use , Interleukin 1 Receptor Antagonist Protein/therapeutic use , Familial Mediterranean Fever/physiopathology , Proteinuria/urine , Reference Values , Time Factors , Turkey , Severity of Illness Index , Blood Sedimentation , Reproducibility of Results , Retrospective Studies , Analysis of Variance , Treatment Outcome , Statistics, Nonparametric , Visual Analog Scale , Amyloidosis/physiopathology , Amyloidosis/drug therapy , Kidney Diseases/physiopathology , Kidney Diseases/drug therapy
Int. braz. j. urol ; 44(6): 1243-1251, Nov.-Dec. 2018. tab, graf
Article in English | LILACS | ID: biblio-975668


ABSTRACT Introduction: We investigated whether Oltipraz (OPZ) attenuated renal fibrosis in a unilateral ureteral obstruction (UUO) rat model. Materials and Methods: We randomly divided 32 rats into four groups, each consisting of eight animals as follows: Rats in group 1 underwent a sham operation and received no treatment. Rats in group 2 underwent a sham operation and received OPZ. Rats in group 3 underwent unilateral ureteral ligation and received no treatment. Group 4 rats were subjected to unilateral ureteral ligation plus OPZ administration. Transforming growth factor beta-1 (TGF-β1), E-cadherin, nitric oxide (NO) and hydroxyproline levels were measured. Histopathological and immunohistochemical examinations were carried out. Results: TGF-β1, NO and E-cadherin levels in the UUO group were significantly higher than the sham group and these values were significantly different in treated groups compared to the UUO group. In rats treated with UUO + OPZ, despite the presence of mild tubular degeneration and less severe tubular necrosis, glomeruli maintained a better morphology when compared to the UUO group. Expressions of α-SMA in immunohistochemistry showed that the staining positivity decreased in the tubules of the OPZ-treated group. Conclusions: While the precise mechanism of action remains unknown, our results demonstrated that OPZ exerted a protective role in the UUO-mediated renal fibrosis rat model highlighting a promising therapeutic potency of Nrf2-activators for alleviating the detrimental effects of unilateral obstruction in kidneys.

Animals , Male , Rats , Pyrazines/therapeutic use , Ureteral Obstruction/complications , NF-E2-Related Factor 2/therapeutic use , Kidney Diseases/drug therapy , Thiones , Thiophenes , Ureteral Obstruction/pathology , Ureteral Obstruction/drug therapy , Fibrosis/etiology , Fibrosis/drug therapy , Immunohistochemistry , Cadherins/blood , Rats, Wistar , Disease Models, Animal , Transforming Growth Factor beta1/blood , Hydroxyproline/blood , Kidney Diseases/etiology , Kidney Diseases/pathology , Nitric Oxide/blood
Arch. argent. pediatr ; 115(5): 294-297, oct. 2017. ilus
Article in Spanish | LILACS, BINACIS | ID: biblio-887380


El absceso renal representa una patología infrecuente en el recién nacido. Puede presentar consecuencias graves: sepsis con alta mortalidad, cicatrices renales y riesgo de enfermedad renal crónica. Se reporta sobre un recién nacido con absceso renal unilateral a Staphylococcus aureus, con cuadro de septicemia, sin otro foco supurativo ni malformación urinaria, que evolucionó adecuadamente con antibióticos endovenosos, sin tratamiento quirúrgico, aunque con cicatrices renales como secuela. A partir de este caso, se analizan las estrategias de diagnóstico, tratamiento y seguimiento del absceso renal en un neonato y se destaca el diagnóstico precoz para evitar cicatrices renales.

Renal abscess is a rare disease in newborn, but severe consequences can occur: sepsis with high mortality, renal scar formation and risk of chronic renal failure. A neonate with unilateral renal abscess due to Staphylococcus aureus is reported, with septicemia, with no other suppurative focus, nor with urinary malformation, with good clinical evolution with intravenous antibiotics and without surgical treatment, but with renal scars sequel. From this case, the strategies of diagnosis, treatment and followup of the renal abscess in a neonate are analyzed, emphasizing the early diagnosis to avoid renal scars.

Humans , Male , Infant, Newborn , Staphylococcal Infections/diagnosis , Staphylococcal Infections/drug therapy , Abscess/diagnosis , Abscess/drug therapy , Kidney Diseases/microbiology , Kidney Diseases/diagnosis , Kidney Diseases/drug therapy
Rev. bras. ter. intensiva ; 27(4): 383-389, out.-dez. 2015. graf
Article in English | LILACS | ID: lil-770045


RESUMO Objetivo: Investigar os efeitos da administração de canabidiol em um modelo de isquemia/reperfusão renal em animais. Métodos: Foi induzida uma lesão renal, por meio de 45 minutos de isquemia renal seguida por reperfusão. Administrou-se canabidiol (5mg/kg) imediatamente após a reperfusão. Resultados: A isquemia/reperfusão aumentou os níveis de interleucina 1 e fator de necrose tumoral, o que foi atenuado pelo tratamento com canabidiol. Além disso, o canabidiol foi capaz de diminuir o dano oxidativo de lipídios e proteínas, mas não os níveis de nitrito/nitrato. A lesão renal após isquemia/reperfusão pareceu ser independente da expressão dos receptores canabidiol-1 e canabidiol-2, já que não houve aumento significante desses receptores após a reperfusão. Conclusão: O tratamento com canabidiol teve um efeito protetor contra a inflamação e o dano oxidativo em um modelo de isquemia/reperfusão renal. Esses efeitos parecem não ocorrer via ativação dos receptores canabidiol-1/canabidiol-2.

ABSTRACT Objective: This work aimed to investigate the effects of the administration of cannabidiol in a kidney ischemia/reperfusion animal model. Methods: Kidney injury was induced by 45 minutes of renal ischemia followed by reperfusion. Cannabidiol (5mg/kg) was administered immediately after reperfusion. Results: Ischemia/reperfusion increased the IL-1 and TNF levels, and these levels were attenuated by cannabidiol treatment. Additionally, cannabidiol was able to decrease lipid and protein oxidative damage, but not the nitrite/nitrate levels. Kidney injury after ischemia/reperfusion seemed to be independent of the cannabidiol receptor 1 and cannabidiol receptor 2 (CB1 and CB2) expression levels, as there was no significant increase in these receptors after reperfusion. Conclusion: The cannabidiol treatment had a protective effect against inflammation and oxidative damage in the kidney ischemia/reperfusion model. These effects seemed to be independent of CB1/CB2 receptor activation.

Animals , Male , Rats , Cannabidiol/pharmacology , Reperfusion Injury/drug therapy , Inflammation/drug therapy , Kidney Diseases/drug therapy , Reperfusion Injury/pathology , Interleukin-1/metabolism , Tumor Necrosis Factor-alpha/metabolism , Rats, Wistar , Oxidative Stress/drug effects , Receptor, Cannabinoid, CB1/metabolism , Receptor, Cannabinoid, CB2/metabolism , Disease Models, Animal , Inflammation/pathology , Kidney Diseases/pathology
Korean Journal of Radiology ; : 1056-1067, 2015.
Article in English | WPRIM | ID: wpr-163297


Immunoglobulin G4 (IgG4)-related kidney disease (IgG4-KD) has recently been demonstrated to be an important part of IgG4-related sclerosing disease (IgG4-SD). However, since IgG4-KD is still relatively unfamiliar to radiologists and physicians as compared to IgG4-SD involving other organs, it could, therefore, be easily missed. In this article, we present a comprehensive pictorial review of IgG4-KD with regards to the imaging spectrum, mimickers, and clinicopathologic characteristics, based on our clinical experience with 48 patients during the past 13 years, as well as a literature review. Awareness of the broad imaging spectrum of IgG4-KD and differential diagnosis from its mimickers will thus facilitate its early diagnosis and treatment.

Adult , Aged , Aged, 80 and over , Autoimmune Diseases/pathology , Female , Humans , Immunoglobulin G/metabolism , Kidney Diseases/drug therapy , Magnetic Resonance Imaging , Male , Middle Aged , Steroids/therapeutic use , Tomography, X-Ray Computed
Article in English | WPRIM | ID: wpr-35685


This study investigated whether tempol, an anti-oxidant, protects against renal injury by modulating phosphatidylinositol 3-kinase (PI3K)-Akt-Forkhead homeobox O (FoxO) signaling. Mice received unilateral ureteral obstruction (UUO) surgery with or without administration of tempol. We evaluated renal damage, oxidative stress and the expression of PI3K, Akt, FoxO3a and their target molecules including manganese superoxide dismutase (MnSOD), catalase, Bax, and Bcl-2 on day 3 and day 7 after UUO. Tubulointerstitial fibrosis, collagen deposition, alpha-smooth muscle actin-positive area, and F4/80-positive macrophage infiltration were significantly lower in tempol-treated mice compared with control mice. The expression of PI3K, phosphorylated Akt, and phosphorylated FoxO3a markedly decreased in tempol-treated mice compared with control mice. Tempol prominently increased the expressions of MnSOD and catalase, and decreased the production of hydrogen peroxide and lipid peroxidation in the obstructed kidneys. Significantly less apoptosis, a lower ratio of Bax to Bcl-2 expression and fewer apoptotic cells in TUNEL staining, and decreased expression of transforming growth factor-beta1 were observed in the obstructed kidneys from tempol-treated mice compared with those from control mice. Tempol attenuates oxidative stress, inflammation, and fibrosis in the obstructed kidneys of UUO mice, and the modulation of PI3K-Akt-FoxO3a signaling may be involved in this pathogenesis.

Animals , Antioxidants/pharmacology , Collagen/metabolism , Cyclic N-Oxides/pharmacology , Fibrosis , Forkhead Transcription Factors/metabolism , Hydrogen Peroxide/metabolism , Kidney Diseases/drug therapy , Lipid Peroxidation , Male , Mice , Mice, Inbred C57BL , Oxidative Stress/drug effects , Phosphatidylinositol 3-Kinases/metabolism , Phosphorylation/drug effects , Proto-Oncogene Proteins c-akt/metabolism , Severity of Illness Index , Signal Transduction/drug effects , Spin Labels , Superoxide Dismutase/metabolism , Ureteral Obstruction/complications
Rev. med. interna ; 17(3): 13-21, ago.-dic. 2013. tab
Article in Spanish | LILACS | ID: biblio-836231


Antecedentes: El fallo renal agudo(FRA) muestra una incidencia enpacientes hospitalizados en unidadde cuidados intensivos (UCI) que vade 4.7% a 9%, con un rango de edadde 50-75 años asociado a múltiplesfactores.Metodología: estudio descriptivoretrospectivo de pacientes en UCI delHospital Roosevelt en el período dediciembre 2012 a marzo del 2013,con el objetivo de determinar laincidencia de FRA. Se definió comoel aumento de creatinina séricamayor de 0.5 mg/dL en 24 horas omás, o mayor del 50% de lacreatinina basal; disminución delfiltrado glomerular del 25% de labasal y/o aumento de la relaciónnitrógeno ureico en sangre: creatininasérica (mayor de 20:1). (18, 19) Seutilizó estadística descriptiva yestadística analítica no paramétricapara el análisis secundario.Resultados: Se incluyeron 29pacientes, 20/29 (69%) fueronmujeres. Se encontró 15/29 (51%)con FRA siendo 12/15 (80%)mujeres. De los pacientes con FRA,5/15 (33%) presentó FRA pre renal,de estos 4/5 (80%) hipovolémico, 1/5(20%) isovolémica y ningunohipervolémico, FRA tipo intrínseco en10/15 (66%), no se identificó ningúncaso de tipo pos-renal. Cuando secomparó el uso de antibióticos,diuréticos, AINES (anti inflamatoriosno esteroideos) y tomografía axialcomputarizada con medio decontraste con el desarrollo o no deFRA, no se encontró diferenciaestadísticamente significativos con p:0.74, OR; 0.76; rango de OR; 0.12 –5, p: 0.74, OR; 0.76; rango de OR;0.12 – 5, p: 0.94, OR; 0.93; rango deOR; 0.1 – 10.1 y p: 0.89, OR; 0.90;rango de OR; 0.2 – 4.5respectivamente...

Humans , Anti-Inflammatory Agents/adverse effects , Kidney Diseases/complications , Kidney Diseases/drug therapy , Renal Insufficiency/complications
Indian J Exp Biol ; 2013 Aug; 51(8): 635-645
Article in English | IMSEAR | ID: sea-149366


An elevated level of serum urea and creatinine was observed in doxorubicin (DOX) treated animals indicating DOX-induced nephrotoxicity. Enhanced lipid peroxidation (LPO) in the renal tissue was accompanied by a significant decrease in the levels of reduced glutathione (GSH), glutathione peroxidase (GPx), glutathione reductase (GR) and catalase (CAT) activities. Administration of lycopene (LycT) extracted from tomato to DOX treated mice showed a significant reduction in serum creatinine and urea levels which were associated with significantly low levels of LPO and significantly enhanced level of GSH and related antioxidant enzymes activity (GPx, GR and CAT) when compared to DOX group. Histopathological analysis revealed severe damage in the renal tissue of DOX treated animals. However, animals pretreated with LycT were observed to have reduced damage. Thus, from present results it may be inferred that lycopene may be beneficial in mitigating DOX induced nephrotoxicity in mice.

Animals , Antibiotics, Antineoplastic/toxicity , Antioxidants/pharmacology , Carotenoids/pharmacology , Catalase/metabolism , Doxorubicin/toxicity , Female , Glutathione/metabolism , Glutathione Peroxidase/metabolism , Glutathione Reductase/metabolism , Immunoenzyme Techniques , Kidney Diseases/chemically induced , Kidney Diseases/drug therapy , Kidney Diseases/pathology , Lipid Peroxidation/drug effects , Lycopersicon esculentum/chemistry , Male , Mice, Inbred BALB C , Oxidative Stress/drug effects , Superoxide Dismutase
Medicina (B.Aires) ; 73(2): 119-126, abr. 2013. tab
Article in Spanish | LILACS | ID: lil-694750


Las vasculitis asociadas a anticuerpos anti-citoplasma de neutrófilos (ANCA) comprenden a un grupo de enfermedades caracterizadas por la inflamación de la pared de pequeños vasos. Analizamos las características epidemiológicas y clínicas en una serie de 47 pacientes: 23 (49%) granulomatosis de Wegener (GW), 15 (32%) poliangeítis microscópica (PAM) y nueve (19%) vasculitis limitada al riñón (VLR). La edad media al inicio de los síntomas fue de 50.7 ± 14.9 años. La manifestación clínica más frecuente fue el compromiso renal en 41 (87%) pacientes, seguido por el pulmonar en 26 (55%) y el otorrinolaringológico en 17 (36%). En 26 (55%) se asoció compromiso renal y pulmonar. La forma clínica más frecuente fue la generalizada en 23 (49%), seguida por la grave en 18 (38%). El 89% presentaron determinaciones de ANCA positivas. Cuatro (8%) no recibieron tratamiento inmunosupresor de inicio. De los 43 que recibieron tratamiento de inicio, 29 (67%) tuvieron remisión completa, con un tiempo de remisión promedio de 35.3 meses. Once (26%) presentaron recaídas, diez (91%) recaídas mayores y uno (9%) menor. Doce (28%) fallecieron, siete en forma temprana y cinco durante la evolución de la enfermedad. Quince (31%) evolucionaron a insuficiencia renal crónica. Los 26 pacientes en seguimiento tuvieron respuesta al tratamiento y 20 (77%) de ellos estaban en remisión al finalizar el estudio. Las vasculitis asociadas a ANCA continúan siendo enfermedades de alta morbilidad y mortalidad, a pesar de las mejorías logradas con los tratamientos inmunosupresores.

Anti-neutrophil cytoplasmic antibody (ANCA) associated vasculitis, comprise a group of diseases characterized by inflammation of the wall of small vessels. We analyzed epidemiological and clinical characteristics in a series of 47 patients, 23 (49%) with Wegener granulomatosis (WG), 15 (32%) with microscopic polyangiitis (MPA) and nine (19%) with renal limited vasculitis (RLV). The mean age at onset of symptoms was 50.7 ± 14.9 years. The most frequent clinical manifestation was renal involvement in 41 (87%), followed by pulmonary manifestations in 26 (55%) and ENT involvement in 17 (36%). In 26 (55%) it presented with simultaneous pulmonary and renal involvement. The most frequent clinical category was the generalized form in 23 (49%), followed by the severe form in 18 (38%). Eighty nine percent of patients had positive ANCA test. Four (8%) received no immunosuppressive treatment. Of the 43 patients who were treated, 29 (67%) achieved complete remission with an average length of remission of 35.3 months. Eleven (26%) had a relapse, ten (91%) had a major relapse and one had a minor relapse. Twelve (28%) patients died, seven died early and five late during the course of the disease. Fifteen (31%) progressed to chronic renal failure. All 26 patients in follow-up had response to treatment and 20 (77%) were in remission at the end of the study. Despite the improvements achieved with immunosuppressive treatments, morbidity and mortality rates in ANCA-associated vasculitis remain high.

Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Young Adult , Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis/immunology , Antibodies, Antineutrophil Cytoplasmic/analysis , Kidney Diseases/immunology , Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis/drug therapy , Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis/mortality , Argentina/epidemiology , Follow-Up Studies , Glucocorticoids/therapeutic use , Immunosuppressive Agents/therapeutic use , Kidney Diseases/diagnosis , Kidney Diseases/drug therapy , Kidney/blood supply , Microscopic Polyangiitis/diagnosis , Microscopic Polyangiitis/drug therapy , Microscopic Polyangiitis/immunology , Remission Induction , Time Factors , Granulomatosis with Polyangiitis/diagnosis , Granulomatosis with Polyangiitis/drug therapy , Granulomatosis with Polyangiitis/immunology
Indian J Exp Biol ; 2013 Feb; 51(2): 149-156
Article in English | IMSEAR | ID: sea-147578


The present study reports protective effect of hydro-alcoholic extract of Luffa acutangula (HAELA) on doxorubicin (DXR) induced cardio and nephro toxicity in mice by studying various serum biomarkers, antioxidants in target organs and histoarchitecture alterations. Pretreatment with HAELA reversed significantly the elevated serum biomarkers, alanine amino transferase, lactate dehydrogenase and creatinine phosphokinase in heart and kidney in DXR treated mice. In addition, HAELA treatment inhibited elevated malondialdehyde formation and restored the depleted glutathione, catalase, superoxide dismutase in heart and kidney tissue. The altered histoarchitecture of heart and kidney tissue due to DXR treatment were also improved with HAELA. The protective activity observed with HAELA on DXR induced cardio and nephrotoxicity in mice was found to be related to its antioxidant property which finally results in membrane stabilization.

Administration, Oral , Animals , Antioxidants/metabolism , Biomarkers/blood , Cardiovascular Diseases/blood , Cardiovascular Diseases/drug therapy , Doxorubicin , Female , Kidney/drug effects , Kidney/pathology , Kidney Diseases/blood , Kidney Diseases/drug therapy , Luffa/chemistry , Male , Mice , Myocardium/pathology , Phytotherapy , Plant Extracts/administration & dosage , Plant Extracts/pharmacology , Plant Extracts/therapeutic use , Staining and Labeling , Toxicity Tests, Acute
Indian J Exp Biol ; 2013 Feb; 51(2): 139-148
Article in English | IMSEAR | ID: sea-147577


To investigate the nephroprotective effect of garlic and elucidate the mechanism by which it prevents the progression of diabetic nephropathy in diabetic rats, diabetes was induced by a single ip injection of streptozotocin (45 mg/kg body weight). Garlic extract (500 mg/kg body weight) and aminoguanidine (1 g/L) were supplemented in the treatment groups. Histopathological examination using H&E, PAS staining and the immunohistochemical analysis of vascular endothelial growth factor (VEGF) and extracellular signal-regulated kinase-1 (ERK-1) expression were performed on kidney sections at the end of 12 weeks. Significant change in both, the urine and serum biochemistry confirmed kidney damage in diabetic animals which was further confirmed by the histological changes such as mesangial expansion, glomerular basement membrane thickening, glycosuria and proteinuria. However, the diabetic animals treated with garlic extract showed a significant change in urine and serum biochemical parameters such as albumin, urea nitrogen and creatinine compared to that of diabetic rats. Further, the garlic supplemented diabetic rats showed a significant decrease in the expression of VEGF and ERK-1 compared to diabetic rats, attenuating mesangial expansion and glomerulosclerosis. Thus, garlic extract rendered nephroprotection in diabetic rats.

Allium/chemistry , Animals , Biomarkers/metabolism , Blood Glucose/metabolism , Creatinine/urine , Diabetes Mellitus, Experimental/blood , Diabetes Mellitus, Experimental/complications , Diabetes Mellitus, Experimental/drug therapy , Diabetes Mellitus, Experimental/enzymology , Glycated Hemoglobin A/metabolism , Immunohistochemistry , Kidney/drug effects , Kidney/enzymology , Kidney/pathology , Kidney Diseases/blood , Kidney Diseases/complications , Kidney Diseases/drug therapy , Kidney Diseases/enzymology , Lipids/blood , Male , Mitogen-Activated Protein Kinase 3/metabolism , Phytotherapy , Plant Extracts/pharmacology , Plant Extracts/therapeutic use , Protective Agents/pharmacology , Protective Agents/therapeutic use , Rats , Rats, Wistar , Urea/urine , Vascular Endothelial Growth Factor A/metabolism
Rev. ANACEM (Impresa) ; 6(2): 104-106, ago. 2012. ilus
Article in Spanish | LILACS | ID: lil-687059


INTRODUCCIÓN: La hidatidosis es una parasitosis endémica en Chile. Los órganos más frecuentemente afectados son hígado y pulmón. Otras localizaciones, tales como la renal son infrecuentes e implican dificultades diagnósticas. PRESENTACIÓN DEL CASO: Mujer de 25 años, sin antecedentes mórbidos, que consulta por dolor lumbar de un mes de evolución, hematuria autolimitada y distensión abdominal, sin hallazgos patológicos al examen físico En sus estudios de laboratorio realizados en Hospital Base de Puerto Montt, Chile, destaca leucocitosis de 13.500 células/mm3 con eosinofilia relativa de 31,4 por ciento, Velocidad de Eritrosedimentación de 74 mm/h y función renal normal. Se solicita ecotomografía abdominal, en la cual se encuentra un quiste renal izquierdo complejo, hallazgo complementado con Tomografía Axial Computada abdomino-pélvico, la cual confirma quiste renal izquierdo de 13 centímetros de diámetro sin aspecto tumoral y quiste en fosa ilíaca izquierda en posición paravesical e hígado sin lesiones. Adicionalmente se realiza radiografía de tórax, la que resulta de aspecto normal. Se realiza Test para Hidatidosis que resulta positivo. Posteriormente, ante la sospecha de hidatidosis renal, se inicia tratamiento con Albendazol 400 mg al día durante 45 días y posteriormente quistectomía renal, evolucionando de forma satisfactoria. Se decide diferir cirugía de quiste paravesical. DISCUSIÓN: La localización renal de un quiste hidatídico es infrecuente y corresponde a menos del 2 por ciento de los casos, el diagnóstico de un quiste complejo renal debe considerar como diagnóstico diferencial la posibilidad de un quiste hidatídico.

INTRODUCTION: Hydatidosis is an endemic parasitic disease in Chile. The main organs affected are the liver and lungs. Other locations, such as the kidneys, are infrequent and involve diagnostic difficulties. CASE REPORT: A 25 year old woman with no previous history of morbid diseases consulting for a one month evolution of lumbar pain, self-limited hematuria and abdominal distension, with no abnormaities on physical examination. In her laboratory studies there was leukocytosis of 13,500 cells/mm3 with relative eosinophilia of 31.4 percent erythrocyte sedimentation rate (ESR) of 74 mm/h and normal renal function. Abdominal ultrasound found a complex left renal cyst, which was complemented with an abdomen and pelvis computerized axial tomography (CT), which confirmed a 13-centimeter left renal cyst without tumor-like appearance. Additionally, chest x-ray shows normal appearance. Enzyme-linked immunosorbent assay (ELISA) for hydatidosis is performed, which results positive. Subsequently, and based on the renal hydatid disease suspicion, she was treated with Albendazole 400 mg per day for 45 days followed by renal cystectomy, evolving satisfactorily. It was decided to defer paravesical cyst surgery. DISCUSSION: Renal hydatid cysts are rare and correspond to less than 2 percent of all cases. The diagnosis of kidney cyst should consider the hydatid cyst as a differential diagnosis.

Humans , Adult , Female , Kidney Diseases/surgery , Kidney Diseases/diagnosis , Echinococcosis/surgery , Echinococcosis/diagnosis , Albendazole/therapeutic use , Anthelmintics/therapeutic use , Diagnosis, Differential , Enzyme-Linked Immunosorbent Assay , Kidney Diseases/drug therapy , Echinococcosis/drug therapy , Tomography, X-Ray Computed
Clinics ; 66(8): 1457-1462, 2011. ilus, tab
Article in English | LILACS | ID: lil-598404


OBJECTIVE: The aim of the present study was to assess the effects of rosuvastatin on renal injury and inflammation in a model of nitric oxide deficiency. METHODS: Male Wistar rats were randomly divided into four groups (n = 10/group) and treated for 28 days with saline (CTRL); 30 mg/kg/day L-NAME (L-name); L-NAME and 20 mg/kg/day rosuvastatin (L-name+ROS-20); or L-NAME and 2 mg/kg/day rosuvastatin (L-name+ROS-2). Systolic blood pressure was measured by plethysmography in the central artery of the tail. The serum total cholesterol, triglycerides, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, creatinine, nitric oxide, interleukin-6, and tumor necrosis factor alpha levels were analyzed. Urine samples were taken to measure the albumin: urinary creatinine ratio. Kidneys were sectioned and stained with hematoxylin/eosin and Masson's trichrome. Immunohistochemical analysis of the renal tissue was performed to detect macrophage infiltration of the glomeruli. RESULTS: The systolic blood pressure was elevated in the L-name but not the L-name+rosuvastatin-20 and L-name+rosuvastatin-2 groups. The L-name group had a significantly reduced nitric oxide level and an increased interleukin-6 and tumor necrosis factor alpha level, albumin: urinary creatinine ratio and number of macrophages in the renal glomeruli. Rosuvastatin increased the nitric oxide level in the L-name+rosuvastatin-2 group and reduced the interleukin-6 and tumor necrosis factor alpha levels, glomerular macrophage number and albumin:urinary creatinine ratio in the L-name+rosuvastatin-20 and L-name+rosuvastatin-2 groups. CONCLUSION: Rosuvastatin treatment reduced glomerular damage due to improvement in the inflammatory pattern independent of the systolic blood pressure and serum lipid level. These effects may lead to improvements in the treatment of kidney disease.

Animals , Male , Rats , Fluorobenzenes/therapeutic use , Hydroxymethylglutaryl-CoA Reductase Inhibitors/therapeutic use , Kidney Diseases/drug therapy , NG-Nitroarginine Methyl Ester/therapeutic use , Nephritis/prevention & control , Nitric Oxide/deficiency , Proteinuria/prevention & control , Pyrimidines/therapeutic use , Sulfonamides/therapeutic use , Blood Pressure , Drug Therapy, Combination/methods , Immunohistochemistry , Interleukins/blood , Kidney Diseases/blood , Nephritis/blood , Nitric Oxide/blood , Plethysmography , Random Allocation , Rats, Wistar
Rio de Janeiro; s.n; 2011. 50 p. ilus.
Thesis in Portuguese | LILACS | ID: lil-657306


Desordens do sistema renal podem ser as causas da hipertensão arterial, a qual pode, por sua vez, causar doenças renais. A pressão sanguínea elevada é muito comum também nas doenças crônicas dos rins, e é, além disso, um conhecido fator de risco para uma mais rápida progressão da falha renal. A incidência de doenças renais crônicas está aumentando no mundo, e há uma grande necessidade de identificar as terapias capazes de deter ou reduzir a progressão da doença. Há crescente evidência de que as estatinas poderiam desempenhar um papel terapêutico. Além disso, tem sido demonstrado que a atividade física melhora a função renal em pacientes. Estudos ultra-estruturais em humanos e em ratos demonstraram a presença de junções gap dentro de todas as células do glomérulo e os podócitos demonstraram conter principalmente conexina-43 (Cx-43). O presente estudo tem como objetivo observar os efeitos da rosuvastatina e da atividade física de baixa intensidade na estrutura e ultra-estrutura renal e na expressão glomerular de Cx-43 em ratos normotensos (WKY) e em ratos espontaneamente hipertensos (SHR). Os ratos foram divididos aleatoriamente em oito grupos: WKY-C: animais normotensos que não receberam rosuvastatina; WKY-ROS: animais normotensos que receberam rosuvastatina 20mg/kg/dia por gavagem orogástrica; SHR-C: animais hipertensos que não receberam rosuvastatina; SHR-ROS: animais hipertensos que receberam rosuvastatina, como descrito no grupo WKY-ROS; SED-WKY: animais normotensos sedentários; EX-WKY: animais normotensos exercitados; SED-SHR: animais hipertensos sedentários; e, EX-SHR: animais hipertensos exercitados. Os animais dos grupos SHR-C, SHR-ROS e SED-SHR apresentaram níveis de pressão arterial maiores que os animais dos grupos WKY-C, WKY-ROS, SED-WKY, EX-WKY e EX-SHR. A massa corporal dos grupos de animais não diferiram significativamente durante o experimento. Não houve diferença nos níveis sanguíneos de uréia, creatinina, ácido úrico e creatinafosfoquinase...

Disorders of the renal system may be the cause of hypertension, which can in turn cause kidney disease. The high blood pressure is also very common in chronic kidney diseases, and is also a known risk factor for faster progression of renal failure. The incidence of chronic kidney disease is increasing woldwide, and there is a great need to identify therapies to stop or slow the progression of the disease. There is growing evidence that statins could play a therapeutic role. Moreover, it has been shown that physical activity improves renal function in patients. Ultrastructural studies in humans and rats have shown the presence of gap junctions in all cells of the glomerular podocytes and also to contain mainly connexin-43 (Cx-43). This study aims to observe the effects of rosuvastatin and low-intensity physical activity on the structure and ultrastructure of kidney and glomerular expression of Cx-43 in normotensive rats (WKY) and spontaneously hypertensive rats (SHR). The rats were randomly divided into eight groups: WKY-C: normotensive animals not receiving rosuvastatin, WKY-ROS: normotensive animals that received rosuvastatin 20mg/kg/day by orogastric gavage, C-SHR: hypertensive animals not receiving rosuvastatin; SHR-ROS: hypertensive rats that received rosuvastatin, as described in ROS-WKY group, WKY-SED: sedentary normotensive, WKY-EX: normotensive rats exercised, SHR-SED: sedentary hypertensive rats, and EX-SHR: hypertensive rats exercised. The animals in groups C-SHR, SHR-SED and SHR-ROS had blood pressure levels higher than the animals in groups WKY-C, ROS-WKY, WKY-SED, EX-SHR and EX-WKY. The body mass of groups of animals did not differ significantly during the experiment. There was no difference in urea, creatinine, uric acid and creatine phosphokinase blood levels among animals of the studied groups. However, there was an increased excretion of 24 hours protein in SHR-C group. There was an increase in the capsule in group SHR-C...

Animals , Male , Female , Rats , /ultrastructure , Exercise/physiology , Fluorobenzenes/therapeutic use , Hypertension/prevention & control , Hydroxymethylglutaryl-CoA Reductase Inhibitors/therapeutic use , Kidney/anatomy & histology , Kidney , Kidney/ultrastructure , Renal Insufficiency, Chronic/epidemiology , Kidney Diseases/drug therapy , Rats, Inbred SHR , Rats, Inbred WKY
Rev. bras. reumatol ; 50(2): 205-210, mar.-abr. 2010. ilus, graf
Article in English, Portuguese | LILACS | ID: lil-552810


As amiloidoses são um grupo heterogêneo de doenças caracterizadas pelo depósito extracelular de uma substância amiloide composta por agregados de proteínas mal acopladas que se depositam longe do sítio de síntese, causando disfunção do órgão-alvo e doença clínica. A forma sistêmica mais comum é a amiloidose A (AA) secundária às infecções e às inflamações crônicas, sendo a artrite reumatoide (AR) a causa mais frequente. O tratamento da amiloidose AA consiste no controle ou na resolução da doença de base. O objetivo do presente estudo é relatar um caso de amiloidose renal secundária em paciente com AR refratária de longa duração que apresentou melhora clínica sustentada após o uso de anti-TNFα (etanercepte).

Amyloidosis is a heterogeneous group of diseases characterized by extracellular deposits of a material composed of aggregates of amyloid - a poorly coupled protein - far from the site of synthesis, causing target organ dysfunction and clinical disease. Systemic amyloidosis A (AA), secondary to infections and chronic inflammation, especially rheumatoid arthritis (RA), is the most common form of amyloid deposition. Treatment of AA consists in the control or resolution of the baseline condition. The objective of the present study was to report a case of secondary renal amyloidosis in a patient with long-term refractory RA who presented sustained clinical improvement after the use of anti-TNFα (etanercept).

Aged , Female , Humans , Amyloidosis/drug therapy , Amyloidosis/etiology , Arthritis, Rheumatoid/complications , Immunoglobulin G/therapeutic use , Kidney Diseases/drug therapy , Kidney Diseases/etiology , Receptors, Tumor Necrosis Factor/therapeutic use , Tumor Necrosis Factor-alpha/antagonists & inhibitors