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Int. j. morphol ; 38(3): 530-535, June 2020. graf
Article in English | LILACS | ID: biblio-1098283


Dysregulated autophagy, whether excessive or downregulated, has been thought to be associated with neurodegenerative disorders including Parkinson's disease. Accordingly, the present study was carried out to investigate whether 3-methyladenine, an autophagy inhibitor, can modulate the effects of rotenone on dopaminergic neurons in primary mesencephalic cell culture. Cultures were prepared from embryonic mouse mesencephala at gestation day 14. Four groups of cultures were treated on the 10th DIV for 48 h as follows: the first was kept as an untreated control, the second was treated with 3-methyladenine alone (1, 10, 100, 200 mM), the third was treated with 20 nM rotenone and the fourth was co-treated with 20 nM rotenone and 3-methyladenine (1, 10, 100, 200 mM). On the 12th DIV, cultured cells were stained immunohistochemically against tyrosine hydroxylase and culture media were used to measure the levels of lactate dehydrogenase. 3methyladenine had no effects on both the survival of dopaminergic neurons and the release of lactate dehydrogenase. Rotenone significantly decreased the number of dopaminergic neurons and increased the levels of lactate dehydrogenase in the culture media. When cultures concomitantly treated with 3-methyladenine and rotenone, 3-methyladenine had no effect against rotenone-induced dopaminergic cell damage and lactate dehydrogenase release into the culture medium. In conclusion, the autophagy inhibitor 3-methyladenine could not modulate rotenone-induced dopaminergic cell damage in primary mesencephalic cell culture.

Se estima que la autofagia desregulada, ya sea excesiva o con baja regulación, está asociada con trastornos neurodegenerativos, incluyendo la enfermedad de Parkinson. En consecuencia, el se realizó este estudio para investigar si la 3metiladenina, un inhibidor de la autofagia,puede modular los efectos de la rotenona en las neuronas dopaminérgicas en el cultivo primario de células mesencefálicas. Los cultivos se prepararon a partir de mesencéfalo de ratón embrionario el día 14 de gestación. Cuatro grupos de cultivos se trataron en el 10º DIV durante 48 h de la siguiente manera: el primer grupo se mantuvo como un control no tratado, el segundo se trató con 3-metiladenina sola (1, 10, 100, 200 mM), el tercer grupo se trató con rotenona 20 nM y el cuarto se trató conjuntamente con rotenona 20 nM y 3-metiladenina (1, 10, 100, 200 mM). En el 12º DIV; las células cultivadas fueron tratadas mediante tinción inmunohistoquímica en tirosina hidroxilasa y se usaron medios de cultivo para medir los niveles de lactato deshidrogenasa. La 3-metiladenina no tuvo efectos tanto en la supervivencia de las neuronas dopaminérgicas como en la liberación de lactato deshidrogenasa. La rotenona disminuyó significativamente el número de neuronas dopaminérgicas y se observó un aumento de los niveles de lactato deshidrogenasa en los medios de cultivo. Cuando los cultivos tratados concomitantemente con 3-metiladenina y rotenona, la 3metiladenina no tuvo efecto contra el daño celular dopaminérgico inducido por la rotenona y la liberación de lactato deshidrogenasa en el medio de cultivo. En conclusión, el inhibidor de la autofagia 3-metiladenina no moduló el daño celular dopaminérgico inducido por la rotenona en el cultivo celular mesencefálico primario.

Animals , Mice , Parkinson Disease , Rotenone/toxicity , Adenine/analogs & derivatives , Autophagy , Mesencephalon , Adenine/pharmacology , Cells, Cultured , Cell Death/drug effects , Dopaminergic Neurons/drug effects , L-Lactate Dehydrogenase/analysis
Chinese Journal of Biotechnology ; (12): 959-968, 2020.
Article in Chinese | WPRIM | ID: wpr-826880


To improve the productivity of L-phenyllactic acid (L-PLA), L-LcLDH1(Q88A/I229A), a Lactobacillus casei L-lactate dehydrogenase mutant, was successfully expressed in Pichia pastoris GS115. An NADH regeneration system in vitro was then constructed by coupling the recombinant (re) LcLDH1(Q88A/I229A) with a glucose 1-dehydrogenase for the asymmetric reduction of phenylpyruvate (PPA) to L-PLA. SDS-PAGE analysis showed that the apparent molecular weight of reLcLDH1(Q88A/I229A) was 36.8 kDa. And its specific activity was 270.5 U/mg, 42.9-fold higher than that of LcLDH1 (6.3 U/mg). The asymmetric reduction of PPA (100 mmol/L) was performed at 40 °C and pH 5.0 in an optimal biocatalytic system, containing 10 U/mL reLcLDH1(Q88A/I229A), 1 U/mL SyGDH, 2 mmol/L NAD⁺ and 120 mmol/L D-glucose, producing L-PLA with 99.8% yield and over 99% enantiomeric excess (ee). In addition, the space-time yield (STY) and average turnover frequency (aTOF) were as high as 9.5 g/(L·h) and 257.0 g/(g·h), respectively. The high productivity of reLcLDH1(Q88A/I229A) in the asymmetric reduction of PPA makes it a promising biocatalyst in the preparation of L-PLA.

L-Lactate Dehydrogenase , Genetics , Lactobacillus casei , Genetics , Phenylpyruvic Acids , Metabolism , Pichia , Genetics , Recombinant Proteins , Genetics , Metabolism
Article in English | WPRIM | ID: wpr-826629


BACKGROUND@#A novel coronavirus called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), first identified in Wuhan, China, has been rapidly spreading around the world. This study investigates the epidemiological and clinical characteristics of coronavirus disease 2019 (COVID-19) patients in Zhejiang Province who did or did not have a history of Wuhan exposure.@*METHODS@#We collected data from medical records of confirmed COVID-19 patients in Zhejiang Province from Jan. 17 to Feb. 7, 2020 and analyzed epidemiological, clinical, and treatment data of those with and without recorded recent exposure in Wuhan.@*RESULTS@#Patients in the control group were older than those in the exposure group ((48.19±16.13) years vs. (43.47±13.12) years, P<0.001), and more were over 65 years old (15.95% control vs. 5.60% exposure, P<0.001). The rate of clustered onset was also significantly higher in the control group than in the exposure group (31.39% vs. 18.66%, P<0.001). The symptom of a sore throat in patients in the exposure group was significantly higher than that in the control group (17.30% vs. 10.89%, P=0.01); however, headache in the exposure group was significantly lower than that in the control group (6.87% vs. 12.15%, P=0.015). More patients in the exposure group had a significantly lower level of lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) than those in the control group. There was no significant difference in any degree of COVID-19 including mild, severe, and critical between the two groups.@*CONCLUSIONS@#From the perspective of epidemiological and clinical characteristics, there was no significant difference between COVID-19 patients with and without Wuhan exposure history.

Adolescent , Adult , Aged , Aspartate Aminotransferases , Blood , Betacoronavirus , Case-Control Studies , Child , Child, Preschool , China , Epidemiology , Coronavirus Infections , Epidemiology , Therapeutics , Female , Humans , Infant , Infant, Newborn , L-Lactate Dehydrogenase , Blood , Male , Middle Aged , Pandemics , Pneumonia, Viral , Epidemiology , Therapeutics , Retrospective Studies , Young Adult
Article in English | WPRIM | ID: wpr-826628


OBJECTIVE@#This study summarizes and compares clinical and laboratory characteristics of 34 patients admitted to the intensive care unit (ICU) for complications from coronavirus disease 2019 (COVID-19) at the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China from Jan. 22 to Mar. 5, 2020.@*METHODS@#A total of 34 patients were divided into two groups, including those who required noninvasive ventilation (NIV) and invasive mechanical ventilation (IMV) with additional extracorporeal membrane oxygenation (ECMO) in 11 patients. Clinical features of COVID-19 patients were described and the parameters of clinical characteristics between the two groups were compared.@*RESULTS@#The rates of the acute cardiac and kidney complications were higher in IMV cases than those in NIV cases. Most patients had lymphocytopenia on admission, with lymphocyte levels dropping progressively on the following days, and the more severe lymphopenia developed in the IMV group. In both groups, T lymphocyte counts were below typical lower limit norms compared to B lymphocytes. On admission, both groups had higher than expected amounts of plasma interleukin-6 (IL-6), which over time declined more in NIV patients. The prothrombin time was increased and the levels of platelet, hemoglobin, blood urea nitrogen (BUN), D-dimer, lactate dehydrogenase (LDH), and IL-6 were higher in IMV cases compared with NIV cases during hospitalization.@*CONCLUSIONS@#Data showed that the rates of complications, dynamics of lymphocytopenia, and changes in levels of platelet, hemoglobin, BUN, D-dimer, LDH and IL-6, and prothrombin time in these ICU patients were significantly different between IMV and NIV cases.

Acute Kidney Injury , Virology , Aged , Aged, 80 and over , Betacoronavirus , Blood Urea Nitrogen , China , Coronavirus Infections , Therapeutics , Extracorporeal Membrane Oxygenation , Female , Fibrin Fibrinogen Degradation Products , Heart Diseases , Virology , Hemoglobins , Hospitalization , Humans , Intensive Care Units , Interleukin-6 , Blood , L-Lactate Dehydrogenase , Blood , Lymphopenia , Virology , Male , Middle Aged , Noninvasive Ventilation , Pandemics , Pneumonia, Viral , Therapeutics , Positive-Pressure Respiration , Prothrombin Time , Retrospective Studies
Braz. dent. sci ; 23(1): 1-8, 2020. tab, ilus
Article in English | LILACS, BBO | ID: biblio-1049962


Objective: Dental composites developed by using nanotechnology in the field of dentistry are widely used in the treatment of anterior and posterior teeth. This study aimed to investigate the cytotoxic effects of dental composites of different particle size on L929 mouse fibroblast cell line by extract test method in vitro. Material and Methods: Composite samples of 8 x 2 mm diameter were prepared by polymerizing with led light device by using glass mod in a sterile cabinet. Composite samples of which surface areas were calculated according to ISO standards (3 cm2 / ml), were incubated for 24 and 72 hours, at 37 o C. cell viability was assessed by 3-[4,5-dimethylthiazole-2- yl]-2,5-diphenyltetrazolium bromide (MTT) assay and cell death was evaluated by the lactate dehydrogenase (LDH) leakage assay. Results: The 1:1 extracts of the composites at the end of 24 hours (except for nanoceramic composite) showed no toxic effect. When the cell viability results of the 1:1 extracts of the composite samples at the end of 72 hours were statistically analyzed, significant differences were found comparing to the control group (p < 0.05). Conclusion: It was observed that the type and size of the filler were effective on the toxicity of the composites, and the composites containing Bis-GMA, TEGDMA, UDMA and Bis EMA monomers in their organic matrix showed acceptable cell viability (70%) as specified by ISO. However, the composites with PEGDMA and BPA monomers in their organic matrix showed poor cell viability, which is below the acceptable level of 70%, and were found to have a toxic effect. (AU)

Objetivo: As resinas compostas desenvolvidas pela nanotecnologia no campo da odontologia são amplamente utilizadas no tratamento de dentes anteriores e posteriores. Este estudo teve como objetivo investigar os efeitos citotóxicos de resinas compostas de diferentes tamanhos de partículas na linha celular de fibroblastos de camundongos L929 pelo método de teste de extrato in vitro. Material e Métodos: Amostras compostas de 8 x 2 mm de diâmetro foram preparadas por polimerização com dispositivo de luz led usando um molde de vidro em um gabinete estéril. Amostras de resinas cujas áreas de superfície foram calculadas de acordo com os padrões ISO (3 cm2 / ml), foram incubadas por 24 e 72 horas, a 37 o C. A viabilidade celular foi avaliada pelo ensaio de brometo de 3- [4,5-dimetiltiazol-2- il] -2,5-difeniltetrazólio (MTT) e a morte celular foi avaliada pelo ensaio de infiltração de lactato desidrogenase (LDH). Resultados: Os extratos 1: 1 dos compósitos ao final de 24 horas (exceto o composto nanocerâmico) não apresentaram efeito tóxico. Quando os resultados de viabilidade celular dos extratos 1: 1 das amostras compostas ao final de 72 horas foram analisados, estatisticamente, foram encontradas diferenças significativas em relação ao grupo controle (p < 0,05). Conclusão: Observou-se que o tipo e tamanho da carga foram eficazes na toxicidade dos compósitos, e os compósitos contendo os monômeros Bis-GMA, TEGDMA, UDMA e Bis EMA em sua matriz orgânica apresentaram viabilidade celular aceitável (70%) como especificado pela ISO. No entanto, os compósitos com monômeros PEGDMA e BPA em sua matriz orgânica apresentaram baixa viabilidade celular, que está abaixo do nível aceitável de 70%, e foram encontrados como tendo um efeito tóxico. (AU)

Animals , Mice , Composite Resins/toxicity , Esthetics, Dental , Fibroblasts , In Vitro Techniques , Cell Line , Cell Survival , Nanoparticles , L-Lactate Dehydrogenase/toxicity
Evid. actual. práct. ambul ; 23(2): e002042, 2020. tab
Article in Spanish | LILACS | ID: biblio-1103530


Este artículo resume las diferentes formas de presentación clínica de la enfermedad COVID-19 causada por el virus SARS-Co-2 documentadas fundamentalmente en las tres principales revisiones sistemáticas disponibles. Entre las manifestaciones clínicas de frecuente aparición se destacan la fiebre (83 %), la tos (60 %) y la fatiga (38 %), seguidas por las mialgias (29 %), el aumento de la producción del esputo (27 %) y la disnea (25 %). Entre los hallazgos de laboratorio,predominan el aumento de los valores de proteína C reactiva (69 %), la linfopenia (57 %) y el aumento de los niveles de lactato-deshidrogenasa (52 %). Respecto de las manifestaciones radiológicas, tienen especial importancia las opacificaciones en vidrio esmerilado (80 %), la neumonía bilateral (73 %) y la afectación de tres lóbulos pulmonares o más (57 %).Si bien la evidencia sintetizada tiene limitaciones, permite una aproximación actualizada a los conocimientos disponibles sobre la clínica de esta nueva enfermedad en la población adulta. (AU)

This article summarizes the different forms of clinical presentation of COVID-19, caused by the SARS-Co-2 virus, synthesizing the information collected mainly by three published systematic reviews. Frequent clinical manifestations include fever(83 %), cough (60 %), and fatigue (38 %), followed by myalgia (29 %), increased sputum production (27 %) and dyspnea(25 %). Among the laboratory findings, the most common are the increase in C-reactive protein values (69 %), lymphopenia (57 %) and the increase in lactate dehydrogenase levels (52 %).. Most remarkable radiological features include ground glass opacifications (80 %), bilateral pneumonia (73 %) and the involvement of three or more lung lobes (57 %). Although the synthesized evidence has limitations, it allows an updated approach to the available knowledge about the clinical symptoms of this new disease in the adult population. (AU)

Humans , Adult , Young Adult , Pneumonia, Viral/physiopathology , Coronavirus Infections/physiopathology , Betacoronavirus/pathogenicity , Pneumonia, Viral/complications , Pneumonia, Viral/etiology , Pneumonia, Viral/diagnostic imaging , Sputum , C-Reactive Protein/metabolism , China , Coronavirus Infections/complications , Coronavirus Infections/etiology , Coronavirus Infections/diagnostic imaging , Cough/diagnosis , Cough/physiopathology , Cough/blood , Dyspnea/diagnosis , Dyspnea/physiopathology , Dyspnea/blood , Fatigue/diagnosis , Fatigue/physiopathology , Fatigue/blood , Pandemics , Fever/diagnosis , Fever/physiopathology , Fever/blood , Myalgia/diagnosis , Myalgia/physiopathology , Myalgia/blood , L-Lactate Dehydrogenase/blood , Lymphopenia/blood
Article in Chinese | WPRIM | ID: wpr-827195


OBJECTIVE@#To investigate the significance of antinuclear antibody and antinuclear antibody spectrum in the stage and prognosis of lymphoma patients.@*METHODS@#79 cases of lymphoma (lymphoma group) treated in the Second Affiliated Hospital of Fujian Medical University and 50 cases of healthy people (control group) were selected. Antinuclear antibodies (ANA) were detected by indirect innmunofluorescence and ANA spectrums were detected by linear Western blot, the expression level of ANA and ANA spectrum in the two groups were analyzed. The lymphoma group was divided into the positive and the negative group according to ANA level, the levels of lactate dehydrogenase (LDH), white blood cell (WBC), disease type, stage and prognosis of the two groups were compared.@*RESULTS@#In the lymphoma group, the positive rate of ANA was 48.1%, while the positive rate was 8.0% in the health control group, both of them showed statistically significant (χ=22.42, P<0.05). ANA fluorescence karyotype in lymphoma group was mainly speckle type. In the Lymphoma group, the positive rate of ANA spectrum was 29.1%, while the positive rate in the control group was 4.0%, both of them showed statistically significant (χ=12.36, P<0.05). The target antigen distribution of ANA spectrum in the lymphoma group was relatively complex, mainly RO52 and SSA, while that in the control group was simple. The positive rate of ANA in lymphoma patients showed increased with age, the titer was mainly 1∶100 low titer positive, the positive rate of ANA in female patients was higher than that in male patients; The average count±standard deviation of LDH and WBC in the ANA positive and negative group were (253.67±255.85) U/L, (218.18±208.34) U/L, (6.34±3.31)×10/L and (6.81±3.91)×10/L respectively, which showed no statistical significance between the two groups (t=0.59 P>0.05; t=0.57 P>0.05); B-cell lymphoma was the main disease in both groups, which accounted for 81.6% (31/38) and 68.3% (28/41) respectively; while in B-cell lymphoma, diffuse large B-cell lymphoma was the main lymphoma. For the patients with B-cell lymphoma, the patients at stage IV in ANA positive group was 58.1% (18/31), while that in the ANA negative group was 28.6% (8 / 28), and both of them showed statistically significant (χ=5.19, P<0.05). Follow-up showed that the survival rate of the patients in ANA negative group was higher than that in ANA positive group, which showed statistically significant difference (P<0.05).@*CONCLUSION@#The postive rate of antinuclear antibody and antinuclear antibody spectrum are higher in lymphoma patients, which have considerable significance for the stage and prognosis of lymphoma treatment.

Antibodies, Antinuclear , Blotting, Western , Female , Humans , L-Lactate Dehydrogenase , Lymphoma , Male , Prognosis
Acta bioquím. clín. latinoam ; 53(4): 477-486, dic. 2019. graf, tab
Article in Spanish | LILACS | ID: biblio-1124025


En este estudio se evaluó el efecto de tomar mate en las pruebas bioquímicas de rutina. Se extrajo sangre a 32 mujeres voluntarias luego de 12 horas de ayuno y a la hora (T1), dos horas (T2) y tres horas (T3) posteriores a la toma de 5 mates. Se estudiaron parámetros hematológicos y analitos de química clínica. Los resultados se analizaron empleando pruebas estadísticas para muestras relacionadas. Se calculó la diferencia porcentual media (DM%) de cada analito en cada hora respecto del valor basal y se comparó con el valor de referencia del cambio (VRC). Una DM% mayor que el VRC se consideró clínicamente significativa. En T1, T2 y T3 los recuentos de neutrófilos, eosinófilos y linfocitos fueron más bajos que en T0, también los niveles de glucosa, urea, creatinina y cistatina C fueron más bajos que en T0, mientras que los valores de proteínas totales, colesterol transportado por lipoproteínas de baja densidad y la actividad enzimática de lactato deshidrogenasa fueron más altos que en T0. En todos los casos los cambios fueron estadísticamente significativos, aunque no lo fueron desde el punto de vista clínico. Tomar 5 mates antes de la flebotomía no interfiere en los resultados de las pruebas bioquímicas de rutina.

In the present study the effect of drinking mate in routine biochemical tests was evaluated. Blood was collected from 32 female volunteers after a 12 h fasting period. In addition, 1 hour (T1), 2 hours (T2), and 3 hours (T3) after drinking 5 mates, blood was collected again. Hematological parameters and clinical chemistry analytes were studied. The results were analyzed using statistical tests for related samples. Mean difference % (MD%) was calculated for each analyte and was further compared with reference change value (RCV). The MDs% higher than RCV were considered clinically significant. At T1, T2, and T3 the count neutrophils, eosinophils and lymphocytes were lower than at T0. Also glucose, urea, creatinine, and cystatin C values were lower than at T0 whereas total proteins, LDL-C, and LD enzymatic activity values were higher than at T0. In all cases, variability was statistically significant but not clinically significant. Drinking 5 mates prior to phlebotomy does not interfere with the results of routine biochemical tests.

Neste trabalho, o efeito de beber chimarrão foi avaliado em testes bioquímicos de rotina. O sangue foi extraído de 32 mulheres voluntárias após 12 horas de jejum, e uma hora (T1), duas horas (T2) e três horas (T3) após a tomada de 5 chimarrões. Parâmetros hematológicos e analitos de química clínica foram estudados. Os resultados foram analisados utilizando testes estatísticos para amostras relacionadas. A diferença percentual média% (DM%) de cada analito em cada hora foi calculada em relação ao valor basal e comparada com o valor de referência da modificação (VRM). Uma DM% maior que o VRM foi considerada clinicamente significativa. Em T1, T2 e T3 as contagens de neutrófilos, eosinófilos e linfócitos foram mais baixas que em T0, Também os níveis de glicose, ureia, creatinina e cistatina C foram mais baixos que em T0, ao passo que os valores de proteínas totais, colesterol transportado por lipoproteínas de baixa densidade e a atividade enzimática de lactato desidrogenase foram mais altos que em T0. Em todos os casos as alterações foram estatisticamente significativas, embora do ponto de vista clínico não o tenham sido. Tomar 5 chimarrões antes da flebotomia não interfere nos resultados dos testes bioquímicos de rotina.

Humans , Urea , Blood , Lymphocytes , Chemistry, Clinical , Fasting , Phlebotomy , Creatinine , Drinking , Cystatin C , Pre-Analytical Phase/methods , Glucose , Lipoproteins, LDL , Referral and Consultation , Rutin , Triiodothyronine , Women , Cholesterol , Data Collection , Eosinophils , Reference Parameters , Pre-Analytical Phase/statistics & numerical data , L-Lactate Dehydrogenase , Neutrophils
Rev. bras. cir. cardiovasc ; 34(5): 517-524, Sept.-Oct. 2019. tab
Article in English | LILACS | ID: biblio-1042055


Abstract Objective: To evaluate the effect of high-dose vitamin C on cardiac reperfusion injury and plasma levels of creatine kinase-muscle/brain (CK-MB), troponin I, and lactate dehydrogenase (LDH) in patients undergoing coronary artery bypass grafting (CABG). Methods: This is a double-blind randomized clinical trial study. Fifty patients (50-80 years old) who had CABG surgery were selected. The intervention group received 5 g of intravenous vitamin C before anesthesia induction and 5 g of vitamin C in cardioplegic solution. The control group received the same amount of placebo (normal saline). Arterial blood samples were taken to determine the serum levels of CK-MB, troponin I, and LDH enzymes. Left ventricular ejection fraction was measured and hemodynamic parameters were recorded at intervals. Results: High doses of vitamin C in the treatment group led to improvement of ventricular function (ejection fraction [EF]) and low Intensive Care Unit (ICU) stay. The cardiac enzymes level in the vitamin C group was lower than in the control group. These changes were not significant between the groups in different time intervals (anesthesia induction, end of bypass, 6 h after surgery, and 24 h after surgery) for CK-MB, LDH, and troponin I. Hemodynamic parameters, hematocrit, potassium, urinary output, blood transfusion, arrhythmia, and inotropic support showed no significant difference between the groups. Conclusion: Vitamin C has significantly improved the patients' ventricular function (EF) 72 h after surgery and reduced the length of ICU stay. No significant changes in cardiac biomarkers, including CK-MB, troponin I, and LDH, were seen over time in each group. IRCT code: IRCT2016053019470N33

Humans , Male , Female , Middle Aged , Aged , Aged, 80 and over , Ascorbic Acid/administration & dosage , Myocardial Reperfusion Injury/prevention & control , Coronary Artery Bypass/methods , Antioxidants/administration & dosage , Arrhythmias, Cardiac/prevention & control , Time Factors , Biomarkers/blood , Myocardial Reperfusion Injury/blood , Double-Blind Method , Reproducibility of Results , Ventricular Function/drug effects , Treatment Outcome , Statistics, Nonparametric , Troponin I/blood , Creatine Kinase, BB Form/blood , Creatine Kinase, MM Form/blood , Hemodynamics/drug effects , Intensive Care Units , L-Lactate Dehydrogenase/blood
Int. braz. j. urol ; 45(3): 629-633, May-June 2019. graf
Article in English | LILACS | ID: biblio-1012332


Abstract Most patients with testicular germ cell tumor present with a painless scrotal mass. We report a 19-year-old patient who presented with neurological complains. Rapid clinical progression to coma was noted during the staging work up. A diagnosis of testicular mixed germ cell tumor with multiorgan metastasis (lymph node, lung, liver and brain) was made. Patients with brain metastasis should receive chemotherapy alone or combined with surgery or radiotherapy. Because the clinical symptoms deteriorated quickly, surgery was used upfront followed by chemotherapy and radiotherapy for the brain tumor. After the first stage of treatment, the clinical symptoms, tumor markers and imaging findings were improved. The residual brain tumor was eliminated by chemotherapy, and only sparse degenerated tumor cells were noted in the brain tissue. Longer follow up is required to assess the impact of our treatment strategy.

Humans , Male , Young Adult , Seizures/pathology , Testicular Neoplasms/pathology , Brain Neoplasms/secondary , Neoplasms, Germ Cell and Embryonal/secondary , Seizures/diagnostic imaging , Testicular Neoplasms/therapy , Testicular Neoplasms/diagnostic imaging , Time Factors , Brain Neoplasms/therapy , alpha-Fetoproteins/analysis , Tomography, X-Ray Computed , Neoplasms, Germ Cell and Embryonal/therapy , Neoplasms, Germ Cell and Embryonal/diagnostic imaging , Chorionic Gonadotropin, beta Subunit, Human/blood , L-Lactate Dehydrogenase/blood
Arq. neuropsiquiatr ; 77(1): 39-46, Jan. 2019. tab, graf
Article in English | LILACS | ID: biblio-983876


ABSTRACT Objective: We investigated the protective effect of the extract of the Camellia japonica L. flower on cerebral ischemia-reperfusion injury in rats. Methods: The rat ischemia-reperfusion injury was induced by middle cerebral artery occlusion for 90 minutes and reperfusion for 48 hours. The animals received an intravenous injection once a day of 20, 40, 80 mg/kg extract of C. japonica for three consecutive days before the ischemia reperfusion. The learning and memory function, the infarct volume, serum malondialdehyde (MDA) level and lactate dehydrogenase activity, and extravasation of immunoglobulin G (IgG) into cerebral parenchyma were assessed as the cell damage index. Results: Pretreatment with extract of C. japonica markedly reduced the infarct volume, serum malondialdehyde level and lactate dehydrogenase activity, and markedly inhibited the extravasation of IgG. Moreover, pretreatment with extract of C. japonica may also inhibit the learning and memory deficits induced by an ischemia-reperfusion injury. Conclusion: It was concluded that pretreatment with extract of C. japonica has a protective effect on cerebral ischemia-reperfusion injury in rats.

RESUMO Objetivo: Investigamos o efeito protetor do extrato da flor de Camellia japonica L. (ECJ) na lesão de reperfusão isquêmica cerebral (I/R) em ratos. Métodos: A lesão de I/R de rato foi induzida por uma oclusão da artéria cerebral média por 90 minutos e reperfusão por 48 horas. Os animais receberam uma injeção intravenosa uma vez ao dia de 20, 40, 80 mg/kg de ECJ por três dias consecutivos antes da I/R. A função de aprendizagem e memória, o volume do infarto, o nível sérico de malondialdeído (MDA), a atividade da desidrogenase láctica e o extravasamento de imunoglobulina (IgG) no parênquima cerebral foram avaliados como índices de dano celular. Resultados: O pré-tratamento com ECJ reduziu acentuadamente o volume do infarto, o nível sérico de MDA e a atividade da desidrogenase láctica, e inibiu marcadamente o extravasamento de IgG. Além disso, o pré-tratamento com ECJ também poderia inibir os déficits de aprendizado e memória induzidos pela lesão de I/R. Conclusão: O pré-tratamento com ECJ tem um efeito protetor contra lesão cerebral de I/R em ratos.

Animals , Male , Female , Plant Extracts/pharmacology , Reperfusion Injury/prevention & control , Brain Ischemia/prevention & control , Neuroprotective Agents/pharmacology , Camellia/chemistry , Swimming/physiology , Time Factors , Immunoglobulin G/blood , Nimodipine/pharmacology , Random Allocation , Reproducibility of Results , Treatment Outcome , Rats, Sprague-Dawley , Disease Models, Animal , L-Lactate Dehydrogenase/analysis , Malondialdehyde/blood
Yonsei Medical Journal ; : 22-29, 2019.
Article in English | WPRIM | ID: wpr-719691


PURPOSE: It is well documented that natural killer (NK) cytotoxicity against hepatocellular carcinoma (HCC) cells is impaired in HCC, which might account for the failure of anti-tumor immune response. miRNAs are considered as important regulators for the development and functions of NK cells. However, the entire role of miR-506 in NK cells remains far from being addressed. MATERIALS AND METHODS: The expressions of miR-506 and signal transducer and activator of transcription 3 (STAT3) mRNA in primary NK cells from HCC patients and healthy controls were detected by quantitative real-time PCR. NK cell cytotoxicity was assessed by CFSE/7AAD cytotoxicity assay and lactate dehydrogenase assay. Luciferase reporter assay, RNA immunoprecipitation assay, and western blot were conducted to confirm the interaction between miR-506 and STAT3. RESULTS: miR-506 expression was downregulated and STAT3 mRNA was upregulated in primary NK cells from HCC patients. Primary NK cells from HCC patients showed remarkably reduced cytotoxicity against SMMC7721 or HepG2 cells. NK cell cytotoxicity was positively correlated with miR-506 expression and negatively correlated with STAT3 mRNA expression. Additionally, miR-506 overexpression enhanced NK cell cytotoxicity against HCC cells, while miR-506 inhibitor showed the reverse effect. Moreover, miR-506 could suppress STAT3 expression by directly targeting 3′-untranslated regions of STAT3. A negative correlation between miR-506 and STAT3 mRNA expression in HCC patients was observed. Mechanistically, overexpressing STAT3 greatly reversed miR-506-mediated promotion of NK cell cytotoxicity against HCC cells. CONCLUSION: miR-506 enhanced NK cell cytotoxicity against HCC cells by targeting STAT3, suggesting that modulating miR-506 expression maybe a promising approach for enhancing NK cell-based antitumor therapies.

Blotting, Western , Carcinoma, Hepatocellular , Hep G2 Cells , Humans , Immunoprecipitation , Killer Cells, Natural , L-Lactate Dehydrogenase , Luciferases , MicroRNAs , Real-Time Polymerase Chain Reaction , RNA , RNA, Messenger , STAT3 Transcription Factor
Article in English | WPRIM | ID: wpr-719457


Systemic lupus erythematosus (SLE) is a chronic autoimmune disorder of an unknown origin. The role of leptospirosis as a triggering factor for SLE is unknown. This paper reports an uncommon case of SLE following a leptospira infection. A 29-year-old female was referred due to fevers, myalgia, and facial edema with rash. Laboratory investigations revealed a hepatic dysfunction, significantly raised lactate dehydrogenase with marked leukopenia and thrombocytopenia. A diagnosis of leptospirosis was confirmed. The patient was treated with antibiotic therapy for leptospirosis. She developed dyspnea after one week. The echocardiogram revealed global hypokinesia with a decreased ejection fraction. A positivity of antinuclear, anti-DNA, and anti-Smith antibodies, together with clinical and laboratory improvement by steroid therapy, led to the diagnosis of SLE. This case highlights the presence of concurrent SLE and leptospirosis. As the symptoms of SLE are similar to leptospirosis, accurate diagnosis through high suspicion is essential for appropriate treatment.

Adult , Antibodies , Diagnosis , Dyspnea , Edema , Exanthema , Female , Fever , Humans , Hypokinesia , L-Lactate Dehydrogenase , Leptospira , Leptospirosis , Leukopenia , Lupus Erythematosus, Systemic , Myalgia , Myocarditis , Thrombocytopenia
Article in English | WPRIM | ID: wpr-719330


PURPOSE: Extranodal natural killer/T-cell lymphoma, nasal type (ENKTL) is a rare subtype of non-Hodgkin lymphoma, and asparaginase-based regimens are the best first-line treatments. Data on the role of specific circulating lymphocyte subsets in the progression of ENKTL are limited. The aim of this study was to investigate the clinical correlation and distribution of circulating absolute CD4+ T-cell counts (ACD4Cs) in ENKTL. MATERIALS AND METHODS: We retrospectively searched medical records for 70 newly diagnosed ENKTL patients treated with pegaspargase-based regimens. Comparison of ACD4Cs as a continuous parameter in different groups was calculated. Univariate and multivariate analyses were used to assess prognostic factors for overall survival (OS) and progression-free survival (PFS). RESULTS: Stage III/IV, B symptoms, elevated lactate dehydrogenase, monocytopenia, high-intermediate and high risk International Prognostic Index (IPI) and Korean Prognostic Index (KPI), high risk Prognostic Index of Natural Killer Lymphoma (PINK), and lower lymphocytes were significantly associated with low ACD4C at diagnosis. With a median follow-up time of 32 months, patients who had an ACD4C < 0.30×109/L had a worse OS. Median OS was 11 months and median PFS was 5 months in the low ACD4C cohort. There were significant differences in both OS and PFS between the two cohorts. Moreover, multivariate Cox analysis identified ACD4Cs as an independent predictor for OS and PFS. CONCLUSION: Low ACD4Cs were associated with poorer survival and could act as a negative predictor for ENKTL patients treated with asparaginase-based regimens.

Cell Count , Cohort Studies , Diagnosis , Disease-Free Survival , Drug Therapy , Follow-Up Studies , Humans , L-Lactate Dehydrogenase , Lymphocyte Subsets , Lymphocytes , Lymphoma , Lymphoma, Extranodal NK-T-Cell , Lymphoma, Non-Hodgkin , Medical Records , Multivariate Analysis , Prognosis , Retrospective Studies , T-Lymphocytes
Article in Chinese | WPRIM | ID: wpr-771874


OBJECTIVE@#To investigate the prognostic value of the serum calcium level corrected by serum albumin (cCA) and corrected serum lactate dehydrogenase (LDH) level for the risk stratification for newly diagnosed multiple myeloma (MM) patients.@*METHODS@#The clinical data and survival of 186 newly diagnosed MM patients admitted to our hospital from June 1, 2015 to November 1, 2017 were collected. The patients's survival time was obtained by telephone and follow-up visits to patients and their families. The value of the prognostic system consisting of cCA levels and LDH levels in the survival time of MM patients was retrospectively analyzed. Moreover, the post-corrected hypercalcemia and high LDH as 2 factors were used for risk stratification, then according to these 2 factors, the MM patients were divided into 3 groups: group 1 (no risk factor), group 2 (1 risk factor) and group 3 (2 risk factors), and the effect of risk factors on the prognosis of MM patients was analyzed.@*RESULTS@#The median follow-up time was 16 months. The cumulative OS rate of the post-corrected hypercalcemia group was lower than that of the non-hypercalcemia group. The 1-year cumulative OS rate in the 2 groups was 79.0%±6.7% and 88.6%±3.0%, the 3-year cumulative OS rate was 53.0%±10.5% and 74.6%±6.6% (P=0.016), respectively. The cumulative OS rate of the high LDH group [LDH >upper limit of normal (ULN), ULN=250 U/L] was lower than that in the normal LDH group. The 1-year cumulative OS rate in the 2 groups was 71.6%±8.6% and 90.0%±2.8%, the 2-year cumulative OS rate was 44.9%±12.1% and 83.1%±4.0%, respectively, and the median OS time was 19 months (95%CI: 15.32-23.34) and not reached (P=0.001). The risk stratification analysis showed that the median OS time of the 3 group was not reached (n=103, 57%), not reached (n=70, 39%) and 17 months (n=7, 4%, 95%CI: 5.19-28.41, P<0.001). Patients with two risk factors had a prognosis worse than patients with 0-1 risk factor.@*CONCLUSION@#The prognostic combination of corrected serum calcium and LDH levels may provide a basis for risk stratification and prognosis in MM patients in clinical practice.

Calcium , Humans , L-Lactate Dehydrogenase , Multiple Myeloma , Prognosis , Retrospective Studies
Article in English | WPRIM | ID: wpr-741870


PURPOSE: The aim of this study was to evaluate the relationships between cytokine and chemokine levels and the clinical severity of Mycoplasma pneumoniae pneumonia. METHODS: A retrospective analysis of clinical and laboratory parameters were performed. Serum levels of interleukin (IL)-6, IL-8, IL-10, IL-18, interferon-γ-inducible protein-10 (IP-10), macrophage inflammatory protein-1β, and tumor necrosis factor-α were measured. The severity of patients' clinical course and radiologic findings were also assessed. RESULTS: Seventy-two patients (35 males and 37 females) with a median age of 3.9 years (range, 1–16 years) were enrolled. Patients with lobar pneumonia (n=29) had significantly higher C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), and IL-18 values than those with broncho-interstitial pneumonia (n=43). However, the cytokine and chemokine values did not differ between the group that was treated with corticosteroids (n=31) and the one that was not (n=41). The CRP, ESR, lactate dehydrogenase (LDH), IL-18, and IP-10 values showed positive correlations with fever duration prior to admission. The CRP and ESR values were positively correlated with IL-18, and LDH, with IP-10 levels. CONCLUSIONS: CRP, ESR, LDH, IL-18, and IP-10 values were associated with the severity of the disease, manifesting lobar pneumonia or prolonged fever duration prior to admission.

Adrenal Cortex Hormones , Blood Sedimentation , C-Reactive Protein , Chemokines , Child , Cytokines , Fever , Humans , Interleukin-10 , Interleukin-18 , Interleukin-8 , Interleukins , L-Lactate Dehydrogenase , Macrophages , Male , Mycoplasma pneumoniae , Mycoplasma , Necrosis , Pneumonia , Pneumonia, Mycoplasma , Retrospective Studies
Article in Korean | WPRIM | ID: wpr-765647


BACKGROUND: Transfusion in neonates and infants can be performed using an electromechanical infusion system that has appropriate accuracy in terms of flow rate, volume, and bolus. However, there are no infusion systems approved for transfusion in Korea. In this study, we evaluate the performance of two electromechanical infusion systems for transfusion in pediatric patients. METHODS: We tested two systems, Baxter and Terumo, using 9 units of leukocyte-filtered red blood cells. The blood samples were delivered through the systems at constant speeds of 10, 30, and 100 mL/hr, and the accuracy in terms of the delivered volume was estimated. Before and after infusion, hemoglobin, hematocrit, plasma hemoglobin, potassium, and lactate dehydrogenase levels were measured in each sample. The percentage of hemolysis (%Hemolysis) was calculated to evaluate the safety of the infusion systems. RESULTS: For Terumo, the mean error rate of the infused volume was less than 5%. We expect that Terumo can transfuse blood at a volume close to the set volume. Further, both infusion systems showed acceptable %Hemolysis levels (mean±standard deviation: Terumo, 0.14±0.04; Baxter, 0.17±0.06). CONCLUSIONS: Both infusion systems can be used safely for transfusion in pediatric patients.

Erythrocyte Transfusion , Erythrocytes , Hematocrit , Hemolysis , Humans , Infant , Infant, Newborn , Korea , L-Lactate Dehydrogenase , Plasma , Potassium
Article in Korean | WPRIM | ID: wpr-765644


In 2018, the general chemistry proficiency testing program of the Korean Association of External Quality Assessment Service consisted of the routine chemistry and urine chemistry programs including 32 and 13 test items, respectively. The test method classification system was revised in the routine chemistry program, and a qualitative test of human chorionic gonadotropin was added in the urine chemistry program. The routine chemistry program was conducted 4 times a year, while the urine chemistry program was conducted twice a year. Statistical analysis data for the test method and reagent companies were reported based on the information and results of the test items provided by each institution. Statistics included the number of participating institutes, mean, standard deviation, coefficient of variation (CV), median, and minimum and maximum values for each group. Each report included tables, histograms, Levey-Jennings charts, and the standard deviation index showing the statistics of each test item. In the routine chemistry program, more than 1,000 institutions performed the 17 test items, and the number is continuously increasing. CV for each test item showed a tendency to increase with decreasing concentration of the proficiency material but was within 10% in most cases. Alkaline phosphatase and lactate dehydrogenase were found to have relatively high CVs because of the differences in results among test methods. In the urine chemistry program, albumin and protein showed high CVs, and the distribution of the test method was different from that of the routine chemistry program.

Academies and Institutes , Alkaline Phosphatase , Chemistry , Chorionic Gonadotropin , Classification , L-Lactate Dehydrogenase , Methods
Cancer Research and Treatment ; : 1557-1567, 2019.
Article in English | WPRIM | ID: wpr-763205


PURPOSE: The extranodal natural killer (NK)/T-cell lymphoma (NKTCL) of non-upper aerodigestive tract (NUAT) was found to have clinical heterogeneity compared with NKTCL of the upper aerodigestive tract (UAT) in small scale studies. We conducted this study in a much larger cohort to analyze the clinical characteristics, prognostic factors, treatment modality, and clinical outcomes of patients with NUAT-NKTCL. MATERIALS AND METHODS: From January 2001 to December 2017, a total of 757 NKTCL patients were identified and included in this study, including 92 NUAT-NKTCL patients (12.2%) and 665 UAT-NKTCLpatients (87.8%). RESULTS: NUAT-NKTCL patients had relatively poorer performance status, more unfavorable prognostic factors, and more advanced stage, compared with UAT-NKTCL patients. The 5-year overall survival (OS) was 34.7% for NUAT-NKTCL, which was significantly worse than UAT-NKTCL (64.2%, p<0.001). The median OS duration was 30.9 months for NUAT-NKTCL. Multivariate analysis showed that presence with B symptoms and elevated serum lactate dehydrogenase independently predicted worse OS. International prognostic index score and prognostic index of natural killer lymphoma score still had prognostic values in NUAT-NKTCL, while the Ann Arbor system could not accurately predict the OS. CONCLUSION: NUAT-NKTCL is a distinctive subtype of NKTCL in many aspects. Patients with NUAT-NKTCL have relatively poorer performance status, more unfavorable prognostic factors, more advanced stage, and poorer prognosis.

Cohort Studies , Humans , L-Lactate Dehydrogenase , Lymphoma , Lymphoma, Extranodal NK-T-Cell , Multivariate Analysis , Population Characteristics , Prognosis
Article in English | WPRIM | ID: wpr-763027


Daidzein isolated from soybean (Glycine max) has been widely studied for its antioxidant and anti-inflammatory activities. However, the protective effects of 7,8,4′-trihydroxyisoflavone (THIF), a major metabolite of daidzein, on 6-hydroxydopamine (OHDA)-induced neurotoxicity are not well understood. In the current study, 7,8,4′-THIF significantly inhibited neuronal cell death and lactate dehydrogenase (LDH) release induced by 6-OHDA in SH-SY5Y cells, which were used as an in vitro model of Parkinson's disease (PD). Moreover, pretreatment with 7,8,4′-THIF significantly increased the levels of superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) and decreased malondialdehyde (MDA) activity in 6-OHDA-induced SH-SY5Y cells. In addition, 7,8,4′-THIF significantly recovered 6-OHDA-induced cleaved caspase-3, cleaved caspase-9, cleaved poly-ADP-ribose polymerase (PARP), increased Bax, and decreased Bcl-2 levels. Additionally, 7,8,4′-THIF significantly restored the expression levels of phosphorylated c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase 1/2 (ERK 1/2), phosphatidylinositol 3-kinases (PI3K)/Akt, and glycogen synthase kinase-3 beta (GSK-3β) in 6-OHDA-induced SH-SY5Y cells. Further, 7,8,4′-THIF significantly increased the reduced tyrosine hydroxylase (TH) level induced by 6-OHDA in SH-SY5Y cells. Collectively, these results suggest that 7,8,4′-THIF protects against 6-OHDA-induced neuronal cell death in cellular PD models. Also, these effects are mediated partly by inhibiting activation of the MAPK and PI3K/Akt/GSK-3β pathways.

Apoptosis , Caspase 3 , Caspase 9 , Catalase , Cell Death , Glutathione , Glycogen Synthase , In Vitro Techniques , JNK Mitogen-Activated Protein Kinases , L-Lactate Dehydrogenase , Malondialdehyde , Neurons , Oxidopamine , Parkinson Disease , Phosphatidylinositol 3-Kinases , Phosphotransferases , Protein Kinases , Soybeans , Superoxide Dismutase , Tyrosine 3-Monooxygenase