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Introducción: El aumento significativo de especies micobacterianas descritas como agentes patógenos implicó la necesidad de implementar métodos de identificación más avanzados que permitan acortar el tiempo diagnóstico. Desde el punto de vista clínico, es importante diferenciar el complejo Mycobacterium tuberculosis (MTBC) del resto de las especies micobacterianas no tuberculosas (MNT), con el fin de aplicar tratamiento específico. Objetivo: Identificar aislados micobacterianos procedentes de muestras clínicas pulmonares y extrapulmonares por las técnicas diagnósticas SD BIOLINE TB Ag MPT64 y Genotype Mycobacterium CM/AS. Material y métodos: Se recibieron 3.604 muestras clínicas procedentes de pacientes sintomáticos durante el período comprendido entre enero 2018­enero 2024. Estas fueron procesadas en el Laboratorio Nacional de Referencia e Investigaciones de Tuberculosis, Lepra y Micobacterias del Instituto de Medicina Tropical "Pedro Kourí" (IPK). Para la identificación de MTBC, se utilizó el test inmunocromatográfico SD TB Ag MPT64; para la identificación de MNT, las técnicas diagnósticas moleculares Genotype Mycobacterium CM/AS. Resultados: Del total de muestras procesadas, se obtuvieron 325 aislados micobac - terianos; 252 (77,53%) identificados como MTBC y 73 (22,45%) especies micobacte- rianas no tuberculosas. De estas, la de mayor frecuencia de aislamiento fue: Myco- bacterium fortuitum 19 (26,02%), Mycobacterium avium 17 (23,80%) y Mycobacterium intracellulare 13 (17,80%). Conclusiones: Los resultados obtenidos reafirman que los métodos de identificación utilizados son adecuados, ambas técnicas logran el acortamiento del tiempo diagnós- tico, lo que permite la implementación temprana del tratamiento adecuado, y así evitar la diseminación de la infección, sobre todo en pacientes con algún tipo de deterioro en su barrera inmunológica.

Introduction: The significant increase in mycobacterial species described as pathoge - nic agents implied the need to implement more advanced identification methods that would shorten the diagnostic time. From a clinical point of view, it is important to diffe- rentiate Mycobacterium tuberculosis complex (MTBC) from the rest of the nontubercu- lous mycobacterial species (NTM), in order to apply specific treatment. Objective: Identify mycobacterial isolates from pulmonary and extrapulmonary clinical samples using the SD BIOLINE TB Ag MPT64 and Genotype Mycobacterium CM/AS diagnostic techniques. Materials and methods: 3,604 clinical samples were received from symptomatic patients during the period between January 2018­January 2024. These were processed at the National Reference and Research Laboratory for Tuberculosis, Leprosy and Mycobacteria of the "Pedro Kourí" Institute of Tropical Medicine (IPK). For the identification of MTBC, the SD TB Ag MPT64 immunochromatographic test was used, and for the iden- tification of NTM, the Genotype Mycobacterium CM/AS molecular diagnostic techniques were used. Results: Of the total samples processed, 325 mycobacterial isolates were obtained; 252 (77.53%) identified as MTBC and 73 (22.45%) nontuberculous mycobacterial species, of which the highest frequency of isolation was: Mycobacterium fortuitum 19 (26.02%), Mycobacterium avium 17 (23. 80%) and Mycobacterium intracellulare 13 (17.80%). Conclusions: The results obtained reaffirm that the diagnostic methods used are adequate, both techniques achieve a shortening of the diagnostic time, which allows the early implementation of the appropriate treatment, thus avoiding the spread of the infection, especially in patients with some type of deterioration in their barrier immuno - logical.

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A cavidade oral é afetada pelo caráter multissistêmico das consequências da Doença Renal Crônica (DRC) e estima-se que cerca de 90% destes pacientes têm sintomas orais. Alterações laboratoriais sanguíneas e salivares são frequentemente observadas e por isso, investigações sobre correlações clínico-laboratoriais são fundamentais para o manejo e tratamento dos pacientes. Neste estudo foi realizada uma revisão sistemática para identificar e avaliar as principais alterações laboratoriais no sangue e na saliva de pacientes portadores de DRC que apresentam manifestações orais. A busca bibliográfica incluiu artigos das bases de dados eletrônicas PubMed, Scopus, Biblioteca Virtual em Saúde, Web of Science, Embase e literatura cinzenta, incluindo estudos caso-controle, transversais e de coorte. A análise do risco de viés seguiu a abordagem QUADAS-2. PROSPERO CRD42022250533 é o registro dessa revisão. As principais alterações laboratoriais encontradas foram o aumento das concentrações sanguíneas e salivares de ureia, creatinina, fosfato e diminuição das concentrações de cálcio e da taxa de fluxo salivar. As concentrações dessas substâncias no sangue e na saliva e a TGF estavam diretamente correlacionadas. Foi observada existência da correlação entre o aparecimento das manifestações orais e as alterações laboratoriais, principalmente xerostomia, disgeusia e hálito urêmico. Em conclusão, a literatura tem revelado que as principais alterações laboratoriais encontradas são aquelas descritas comumente na rotina laboratorial, que as concentrações dessas substâncias no sangue e na saliva estão diretamente correlacionadas com a TFG, e existe correlação entre o aparecimento das manifestações orais e as alterações laboratoriais. Grandes oportunidades estão abertas para a investigação sobre de novos marcadores.

The oral cavity is affected by the multisystemic nature of the consequences of Chronic Kidney Disease (CKD) and it is estimated that around 90% of these patients present oral symptoms. Blood and salivary laboratory changes are frequently observed and, therefore, investigations of clinical-laboratory correlations are essential for the management and treatment of these patients. This study was carried out as a systematic review to identify and evaluate the main laboratory changes in the blood and saliva of patients with CKD who present oral manifestations. The bibliographic search included articles from the electronic databases PubMed, Scopus, Virtual Health Library, Web of Science, Embase and gray literature, including case-control, cross-sectional and cohort studies. The risk of bias analysis advanced the QUADAS-2 approach. PROSPERO CRD42022250533 is the record of this review. The main laboratory changes found were an increase in blood and salivary concentrations of urea, creatinine, phosphate and a decrease in calcium concentrations and salivary flow. The concentrations of substances in blood and saliva and TGF were directly correlated. The existence of the manifestation was observed between the appearance of oral manifestations and laboratory changes, mainly xerostomia, dysgeusia and uremic breath. In conclusion, the literature revealed that the main laboratory changes found are those commonly described in laboratory routine, that the concentrations of these problems in blood and saliva are directly correlated with GFR, and there is a manifestation between the appearance of oral manifestations and laboratory changes. . Great opportunities are open for the investigation of new markers.

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Introducción. La fase pre-analítica es un componente esencial en el algoritmo de análisis clínicos, que conlleva una serie de pasos y procedimientos. Objetivo. Describir el nivel de cumplimiento de los indicadores preanalíticos en las áreas de química y hematología del laboratorio clínico del Hospital Distrital Inmaculada Concepción. Materiales y método. Se aplicó un diseño no experimental con un enfoque mixto de corte transversal y alcance descriptivo. Se incluyeron tres bioquímicos y cien pacientes mediante un muestreo por conveniencia. Los datos fueron obtenidos por observación estructurada a pacientes y entrevista semiestructurada a los funcionarios del hospital. Resultados. El nivel de desempeño de los bioquímicos con relación a los indicadores preanalíticos presenta aspectos tanto positivos como negativos, de los cuales resalta el aspecto positivo en un 61% y el 39% corresponde a aspectos considerados como deficientes. Las dificultades existentes son la identificación correcta del paciente y la comunicación entre el médico tratante y/o técnico con ellos, los cuales en su gran mayoría no tienen conocimiento de las condiciones en las cuales deben presentarse para un examen médico. Conclusión. La adecuada realización de las acciones correctas, como la calidad de las mismas, en la fase pre-analítica se relaciona de manera directa con los resultados que se obtienen, que luego serán utilizados por los profesionales solicitantes para toma de decisiones, por lo que es un eslabón inicial que afecta a todo el proceso posterior. Palabras clave: calidad de atención en salud; laboratorio; fase preanalítica

Introduction. The pre-analytical phase is a vital component of the clinical analysis algorithm. This involves a series of steps and procedures. Objective. To describe the level of compliance with the preanalytical indicators in the areas of chemistry and haematology ofthe clinical laboratory at a local Hospital in Caaguazú. Materiales y método. Anon-experimental design and a mixed cross-sectional approach were applied. Three biochemists and one hundred patients were includer. Results. Our data was obtained by structured observation to patients and semi-structured interviews tothree biochemists. The level of performance of the biochemists about the pre-analytical indicators presents both positive and negative aspects, of which the positive aspect stands out in 61% and 39% corresponds to aspects considered as deficient. The existing difficulties are the correct identification of the patient and the communication between the treating physician and/or technician, most of whom are unaware ofthe conditions in which they should present themselves for a medical examination. Conclusion. The correct performance, as well as their quality, in the pre-analytical phase, is directly related to the results obtained, which the requesting professionals will later use for decision-making, so it is an initial link that affects the entire subsequent process and must always be considered essential. Keywords: quality of health care; laboratories; pre-analytical phase

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El proceso de desarrollo de nuevos métodos diagnósticos, tratamientos y cualquier desarrollo en medicina es un proceso que puede tardar muchos años y requiere de muchos recursos. La medicina traslacional surge de la necesidad de acelerar estos procesos aplicando los conocimientos de ciencias básicas y la clínica en conjunto para la búsqueda de soluciones medicas con un enfoque holístico. Acelerando estos procesos y evitando el uso de recursos sin enfoques aplicables en la clínica permite dar soluciones prácticas, rápidas y con impacto en salud pública. En un sentido más amplio y avanzado podemos decir que la medicina traslacional se trata de ir a la cama del paciente desde lo que se obtienen en el laboratorio y viceversa. Catalizando la unión de los conceptos generados en los laboratorios de ciencias básicas con los avances tecnológicos e innovaciones producidas en todos los ámbitos de la investigación científica multidisciplinaria para generar soluciones clínicas en base a problemáticas identificadas en la clinica(1).

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Background@#According to the guidelines of the Department of Health (DOH)’s Health Facilities and Services Regulatory Bureau (HFSRB), accreditation of drug testing laboratories (DTLs) requires annual participation in a proficiency testing (PT) program. Since 2009, the National Reference Laboratory for Environmental and Occupational Health, Toxicology and Micronutrient Assay of the East Avenue Medical Center (NRL-EAMC) has conducted the PT program for DTLs.@*Objectives@#This article aims to provide a general overview of the PT program conducted for screening drug testing laboratories (SDTLs) and to examine data on laboratories’ participation and performance in the PT program.@*Methodology@#Laboratories registered for the PT program were given ten 3-mL synthetic urine specimens which may or may not contain drugs of abuse such as methamphetamine and tetrahydrocannabinol at or above the cut-off level. Laboratories analyzed the PT specimens using immunoassay test kits. The results of the analysis were reported back to NRL-EAMC. The performance of the laboratories in the PT depends on the number of incorrect responses.@*Results@#For ten years (2009-2019), 1102 ± 188 laboratories annually participated in the program. The mean passing rate was 96.6 ± 4.8%. The number of laboratories which initially failed the PT program significantly decreased from 2009 (15.1%) to 2012 (1.5%). From 2013 to 2019, only below 2.5% of the participating laboratories initially failed the PT. On average, 48.4 ± 18.4% of the laboratories achieved an excellent performance, 34.0 ± 13.6% had a highly satisfactory performance, and 14.3 ± 5.4% got an acceptable performance.@*Conclusion@#The continued decreasing number of laboratories which failed the PT signifies the improvement of laboratories in urine drug testing. In general, some laboratories participating in the PT for the first time are the ones which initially fail the PT which could be due to a lack of experience in handling PT test items. The PT program highlights the effectiveness of quality control procedures being implemented in a drug testing laboratory.

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Este trabalho tem o objetivo de analisar as concepções de maternidade para mulheres inférteis de diferentes níveis socioeconômicos que estão em tratamento de reprodução assistida. Trata-se de um estudo qualitativo, descritivo, que utilizou como instrumento uma entrevista semiestruturada e contemplou temas como o significado de família, desejo/expectativas sobre filho e gestação e expectativas sobre a maternidade. Participaram da pesquisa 48 mulheres inférteis acima de 35 anos que usam tecnologias de reprodução assistida de alta complexidade em instituições privada e pública. Os dados foram tratados pela análise de conteúdo em que emergiram os temas: representações sociais da família; representações sociais da maternidade; expectativas com a gestação e os modelos maternos; e o filho imaginado. As participantes representaram a família de forma positiva, como um sistema de suporte, de fundação e origem de amor, configurando-a como um laço social. Por outro lado, as concepções de família com base na consanguinidade também estiveram presentes, representando a família pela perpetuação da espécie e pela importância do laço biológico. A maternidade foi marcada por significativa idealização, sendo vista como um papel gratificante e de realização da feminilidade. O peso da cobrança social para procriar também foi sentido como um dever a cumprir e que, na impossibilidade de se realizar, gera sentimentos de inferioridade, menos-valia, impotência e inadequação perante a sociedade, o que reforça o estigma da infertilidade. Tais resultados apontam a importância de reflexões sobre o papel da mulher na nossa cultura, visto que a maternidade é ainda utilizada como medida para o sucesso ou fracasso feminino. Faz-se necessário também refletir sobre a possibilidade da maior inserção do trabalho psicológico na reprodução assistida, visto a carga emocional e social envolvidas nesse processo.(AU)

This study aimed to analyze the conceptions of motherhood for infertile women from different socioeconomic levels who are undergoing assisted reproduction treatment. This is a qualitative and descriptive study that used a semi-structured interview as an instrument and included topics such as the meaning of family and desires/expectations about the child, pregnancy, and motherhood. A total of 48 infertile women over 35 years of ages using high-complexity assisted reproductive technologies in private and public institutions participated in this research. The data were treated by content analysis in which the following themes emerged: family social representations; social representations of motherhood; expectations with pregnancy and maternal models; and the imagined son. Participants represented the family in a positive way as a support system and the foundation and origin of love, embracing the family as a social bond. On the other hand, the family concepts based on inbreeding were also present, representing the family by perpetuation of the species and the importance of biological bonds. Motherhood was marked by significant idealization, being seen as a gratifying role and the fulfillment of femininity. The weight of the social demand to procreate was also felt as a duty to be fulfilled that, in the impossibility of carrying it out, generates feelings of inferiority, worthlessness, impotence, and inadequacy toward society, which reinforce the stigma of infertility. Results point to the necessary reflections on the role of women and our culture since Motherhood is still used as a measure of female success or failure. They also point to a reflection on the possibility of greater inclusion of psychological work in assisted reproduction given the emotional and social burden involved in this process.(AU)

Este estudio tuvo como objetivo analizar las concepciones de maternidad de mujeres infértiles, de diferentes niveles socioeconómicos, que se encuentran en tratamiento de reproducción asistida. Se trata de un estudio cualitativo, descriptivo, que utilizó como instrumento una entrevista semiestructurada e incluyó temas como el sentido de la familia, deseos/expectativas sobre el hijo y el embarazo y expectativas sobre la maternidad. Participaron en la investigación un total de 48 mujeres infértiles, mayores de 35 años, usuarias de tecnologías de reproducción asistida de alta complejidad en instituciones públicas y privadas. Los datos se sometieron a análisis de contenido del cual surgieron los temas: representaciones sociales familiares; representaciones sociales de la maternidad; expectativas con el embarazo y modelos maternos; hijo imaginado. Las participantes representaron a la familia de manera positiva, como sistema de apoyo, fundamento y origen del amor, configurándola como vínculo social. Por otro lado, también estuvieron presentes las concepciones familiares basadas en la consanguinidad, representando a la familia para la perpetuación de la especie y la importancia del vínculo biológico. La maternidad estuvo marcada por una importante idealización, vista como un rol gratificante y de realización de la feminidad. También se sintió el peso de la demanda social de procrear como un deber que cumplir y que, ante la imposibilidad de realizarlo, genera sentimientos de inferioridad, desvalorización, impotencia e inadecuación en la sociedad, lo que refuerza el estigma de la infertilidad. Por tanto, son necesarias reflexiones sobre el papel de la mujer en nuestra cultura, ya que la maternidad se sigue utilizando como medida del éxito o fracaso femenino. También se reflexiona sobre la posibilidad de una mayor inclusión del trabajo psicológico en la reproducción asistida dada la carga emocional y social que implica este proceso.(AU)

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El trabajo desarrollado en los laboratorios, es importante no solo por su rol en el diagnóstico de las enfermedades transmisibles como la tuberculosis, sino también en el control de la evolución del tratamiento de los pacientes, así como la evaluación epidemiológica y operacional. Tiene como función principal, la detección de fuentes de infección tuberculosa a través de métodos como pruebas moleculares, cultivo, baciloscopia y prueba de flujo lateral para lipoarabinomanano (LF-LAM). La elección de la técnica más conveniente y su estandarización permite la obtención de resultados comparables a lo largo de todo el país, además de facilitar la capacitación, así como la ampliación de la cobertura. El propósito de los presentes lineamientos, es proporcionar al personal de laboratorio clínico del Sistema Nacional Integrado de Salud (SNIS) y de otras instituciones prestadoras de servicios de salud, información sobre los procedimientos técnicos aplicables para el diagnóstico bacteriológico de la tuberculosis

The work carried out in the laboratories is important not only for its role in the diagnosis of communicable diseases such as tuberculosis, but also in the control of the evolution of the treatment of patients, as well as the epidemiological and operational evaluation. Its main function is to detect sources of tuberculosis infection through methods such as molecular tests, culture, bacilloscopy and lateral flow test for lipoarabinomanan (LF-LAM). The choice of the most appropriate technique and its standardization allows for comparable results throughout the country, in addition to facilitating training, as well as expanding coverage. The purpose of these guidelines is to provide clinical laboratory staff with

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OBJECTIVE@#Taking artificial hip joint test as the object, integrating ISO/IEC 17025 and GLP, and establishing a new set of management requirements for test influence factors.@*METHODS@#The requirements of ISO/IEC 17025 and GLP regulations for influencing factors were compared and analyzed, the similarities and differences were found, and the two were integrated to formulate new management requirements for each influencing factor.@*RESULTS@#From the personnel, equipment, materials, methods, reports, filing and other factors, a set of management requirements in line with ISO/IEC 17025 and GLP was formulated, so that the laboratory can provide the objective, real and accurate test data for medical equipment manufacturers and regulatory authorities.@*CONCLUSIONS@#It can improve the test quality of the laboratory and ensure the authenticity and reliability of the test data and conclusions.

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OBJECTIVE@#To compare the prevalence of chromosomal aneuploidies and pregnancy outcomes of D5 and D6 blastocysts subjected to preimplantation genetic testing for aneuploidy (PGT-A).@*METHODS@#Clinical and laboratory data of 268 couples who underwent PGT-A at the Reproductive Center of the First Affiliated Hospital of Zhengzhou University from September 2018 to September 2020 were collected. The prevalence of chromosomal aneuploidies and pregnancy outcomes of D5/D6 biopsied blastocysts were compared.@*RESULTS@#Compared with D6 blastocysts, the euploidy rate of D5 blastocysts was significantly higher (49.1% vs. 41.1%, P = 0.001 1), whilst their aneuploidy rate was significantly lower (50.9% vs. 58.9%, P = 0.001 1). The rate of numerical abnormalities of D6 blastocysts was significantly higher than that of D5 blastocysts (27.9% vs. 20.2%, P = 0.000 5). For patients under 35 years old, the euploidy rate of D5 blastocysts was significantly higher than that of D6 blastocysts (53.8% vs. 44.3%, P = 0.001), whilst the numerical abnormality rate was significantly lower (16.3% vs. 23.9%, P = 0.001). For both D5 and D6 blastocysts, the euploidy rates for patients <= 35 were significantly higher than those for > 35. The elder group had the lowest rates for aneuploidies and live births. Compared with those receiving D6 blastocysts transplantation, the pregnancy rate, implantation rate and live birth rate for those receiving thawed D5 blastocysts transplantation were significantly higher (60.2% vs.37.0%, P = 0.000 3; 59.1% vs.37.0%, P = 0.000 6; 47.7% vs. 28.3%, P = 0.002).@*CONCLUSION@#For patients undergoing PGT-A, the chromosomal euploidy rate for D5 blastocysts is higher than that for D6 blastocysts, and the clinical outcome of D5 blastocysts with normal signal is better than that of D6 blastocysts. Elder patients have a higher rate of aneuploidies.

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Objective: To characterize the serum bile acid profiles of healthy children in Zhejiang Province. Methods: A cross-sectional study was conducted on 245 healthy children who underwent imaging and laboratory biochemical tests during routine physical examinations at the Children's Hospital of Zhejiang University School of Medicine from January 2020 to July 2022. Overnight fasting venous blood samples were collected, and the concentrations of 18 individual bile acids in the serum were accurately quantitated using tandem mass spectrometry. The concentration difference of bile acid were compared between different genders and to explore the correlation between age and bile acid levels. Used the Mann-Whitney U test for intergroup comparison and Spearman test to correlation analysis. Results: A total of 245 health children with a age of 10 (8, 12) years including 125 boys and 120 girls. There were no significant differences in levels of total bile acids, primary and secondary bile acids, free and conjugated bile acids between the two gender groups (all P>0.05). The serum concentrations of ursodeoxycholic acid and glycoursodeoxycholic acid in girls were significantly higher than those in boys (199.0 (66.9, 276.5) vs. 154.7 (49.3, 205.0) nmol/L, 274.0 (64.8, 308.0) vs. 181.0 (43.8, 209.3) nmol/L, Z=2.06, 2.71, both P<0.05). The serum taurolithocholic acid in both boys and girls were positively correlated with age (r=0.31, 0.32, both P<0.05). The serum chenodeoxycholic acid and glycochenodeoxycholic acid in the boys group were positively correlated with age (r=0.20, 0.23, both P<0.05), whereas the serum tauroursodeoxycholic acid in the girls group was negatively correlated with age (r=-0.27, P<0.05), and the serum cholic acid was positively correlated with age (r=0.34, P<0.05). Conclusions: The total bile acid levels are relatively stable in healthy children in Zhejiang province. However, individual bile acids showed gender differences and were correlated with age.

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Resumo O artigo reflete sobre as justificativas do projeto "Lugar de memória: história e vida dos trabalhadores técnicos da Fiocruz", uma iniciativa de educação patrimonial que relaciona o patrimônio arquitetônico do campus Manguinhos com a memória e as muitas histórias dos auxiliares de laboratório que atuaram no Instituto Oswaldo Cruz em seus trinta primeiros anos de existência. O propósito do projeto foi estabelecer um lugar de memória para os trabalhadores técnicos da instituição, compondo um diálogo entre passado e presente da história da Fiocruz e integrando espaços virtuais a ambientes históricos.

Abstract This article reflects on the justifications for the "Lugares de Memória: história e vida dos trabalhadores técnicos da Fiocruz" project, a heritage education initiative linking the architectural heritage of the Manguinhos campus to the memory and many histories of the laboratory assistants who worked at the Oswaldo Cruz Institute (Fiocruz) during its first thirty years of existence. The objective was to establish a space for memory for the institution's technical staff by creating a dialog between the past and present in the history of Fiocruz and integrating virtual spaces and historical environments.

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Abstract In this study, the manufacturing process of lamivudine (3TC) and zidovudine (AZT) tablets (150+300 mg respectively) was evaluated using statistical process control (SPC) tools. These medicines are manufactured by the Fundação para o Remédio Popular "Chopin Tavares de Lima" (FURP) laboratory, and are distributed free of charge to patients infected with HIV by the Ministry of Health DST/AIDS national program. Data of 529 batches manufactured from 2012 to 2015 were collected. The critical quality attributes of weight variation, uniformity of dosage units, and dissolution were evaluated. Process stability was assessed using control charts, and the capability indices Cp, Cpk, Pp, and Ppk (process capability; process capability adjusted for non-centered distribution; potential or global capability of the process; and potential process capability adjusted for non-centered distribution, respectively) were evaluated. 3TC dissolution data from 2013 revealed a non-centered process and lack of consistency compared to the other years, showing Cpk and Ppk lower than 1.0 and the chance of failure of 2,483 in 1,000,000 tablets. Dissolution data from 2015 showed process improvement, revealed by Cpk and Ppk equal to 2.19 and 1.99, respectively. Overall, the control charts and capability indices showed the variability of the process and special causes. Additionally, it was possible to point out the opportunities for process changes, which are fundamental for understanding and supporting a continuous improvement environment.

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Abstract Development of insecticides resistance mainly hinge with managements techniques for the control of Jassid, Amrasca biguttutla biguttutla. Five insecticides were applied against field collected and laboratory rared jassid populations during the years of 2017 to 2019 to profile their resistance level against field population of jassid through leaf dip method. Very low resistance level was found in jassid against confidor whereas high level of resistance was observed by pyriproxyfen against other test insecticides. Gradual resistance was observed against diafenthiuron. It is concluded that for the management of Jassid repetition of same insecticide should be avoided. The use of confidor may be reduced to overcome resistance against Jassid.

Resumo Desenvolvimento da resistência a inseticidas principalmente em dobradiça com técnicas de manejo para o controle de jassid, Amrasca biguttutla biguttutla. Cinco inseticidas foram aplicados contra populações de jassídeos coletados em campo e em laboratório durante os anos de 2017 a 2019 para traçar o perfil de seu nível de resistência contra populações de jassídeos em campo através do método de imersão nas folhas. Nível de resistência muito baixo foi encontrado em jassid contra confidor, enquanto alto nível de resistência foi observado por piriproxifeno contra outros inseticidas de teste. Resistência gradual foi observada contra o diafenthiuron. Conclui-se que, para o manejo do jassid, a repetição do mesmo inseticida deve ser evitada. O uso de confidor pode ser reduzido para superar a resistência contra jassid.

ABSTRACT

Abstract Behavioral lab bioassays involving termites must be promptly performed to allow intended observations prior to death from dissecation, typical of these soft-bodied insects. To this end, topic markers have been proposed as an alternative to histological stains which, while not always toxic are inevitably lengthy to apply. Among recommended topic markers, gouache is easy to apply, dries out quickly, but it is known affect termites in the long run, being suitable only to short-term bioassays. Its alternative, colored glue, is also easy to apply, but it takes long to dry and it is too dense and heavy, being thus prone to affect termite walking patterns. Here we tested a mix of gouache and colored glue aiming to combine the qualities of both into a suitable topical marker for Cornitermes cumulans termites. Similar patterns of survival presented by marked and unmarked termites ruled out concerns about toxicity of this mixture. Such results were consistent across distinct group densities evidencing that the mixture does not interfere with, nor it is affected by, crowding effects. Because crowding regulates interindividual interactions and these underlie most behaviors, the mixture can be thought to be suitable to behavioral studies. We argue that this 1:2 glue:gouache mixture is an excellent alternative to mark termites for lab bioassays. Being atoxic, cheap, easy to apply, and non-invasive, this mixture may happen to be useful not only for termites but also in bioassaying other similarly soft-bodied insects.

Resumo Bioensaios comportamentais em laboratório com cupins devem ser realizados rapidamente a fim de garantir observações antes da morte por dissecação, típico desses insetos de corpo mole. Para este fim, marcadores tópicos têm sido propostos como uma alternativa para marcadores histológicos que, embora nem sempre tóxico, possuem uma aplicação demorada. Entre os marcadores tópicos recomendados, tinta guache é de fácil aplicação, rápida secagem, porém afeta os cupins em bioensaios longos, sendo adequado apenas para bioensaios curtos. Sua alternativa, cola colorida, também é de fácil aplicação mas leva muito tempo para secar e é muito denso e pesado, afetando os padrões de caminhamento dos cupins. No presente estudo, nós testamos uma mistura de tinta guache e cola colorida objetivando combinar as qualidades de ambos os marcadores tópicos em um marcador tópico adequado para Cornitermes cumulans. Padrões similares de sobrevivência entre cupins marcados e controle indicam a ausência de toxicidade na mistura de tinta guache e cola colorida. Tais resultados são consistentes em grupos de densidades distintas, o que comprova que a mistura não interfere, nem sofre efeitos de aglomeração. Uma vez que a aglomeração regula as interações inter-individuais e afetam a maioria dos comportamentos, a mistura pode ser adequada para estudos comportamentais. Nós argumentamos que a mistura de tinta guache e cola (1:2) é uma excelente alternativa como marcador tópico em cupins para bioensaios em laboratório. Sendo atóxico, barato, fácil de aplicar e não invasivo, esta mistura pode ser útil não só para os cupins, mas também em bioensaios com outros insetos de corpo mole.

ABSTRACT

O hospital constitui-se como um contexto em que a urgência subjetiva pode vir a se apresentar de forma frequente, instaurando, para cada sujeito, uma vivência de angústia. O objetivo desta pesquisa foi investigar as possibilidades para uma clínica das urgências subjetivas no contexto de um hospital universitário em Salvador, considerando as vivências em uma residência multiprofissional. Trata-se de uma pesquisa qualitativa de caráter exploratório, em que se realizou revisão teórica sobre o tema e se construiu um caso clínico, sob orientação psicanalítica. A escolha do caso baseou-se na escuta clínica ao longo dos atendimentos e da atuação em equipe multiprofissional, considerando os impasses ao longo do tratamento. Foram utilizados registros documentais produzidos pela psicóloga residente ao longo dos atendimentos, que ocorreram durante três meses. Os resultados apontam para as contribuições da escuta psicanalítica no tratamento das urgências e na atuação em equipe multiprofissional no contexto hospitalar. A subjetivação da urgência permitiu, no caso em questão, um tratamento pela palavra do que havia incidido diretamente no corpo como fenômeno. Conclui-se pela relevância em discutir o tema da urgência e suscitar novas pesquisas, reintroduzindo no contexto hospitalar a questão sobre a subjetividade.(AU)

Hospitals are contexts in which subjective urgency can frequently materialize, triggering an experience of anguish for each subject. Hence, this research investigates the possibilities of establishing a subjective urgency clinic at a university hospital in Salvador, considering the experiences in a multidisciplinary residence. A qualitative, exploratory research was conducted by means of a theoretical review on the topic and construction of a clinical case, under psychoanalytical advisement. The case was chosen based on clinical listening during the sessions and performance in a multidisciplinary team, considering the obstacles for long-term treatment. Data were collected from documentary records produced by the resident psychologist during three months. Results point to the contributions of psychoanalytic listening to treating subjective urgencies and to the performance of a multidisciplinary team in the hospital context. In the case in question, subjectivation of urgency allowed a treatment through the word of affecting phenomenon. In conclusion, discussing urgency and conducting further research, are fundamental to reintroduce subjectivity in the hospital context.(AU)

El hospital es un contexto en el que frecuentemente se puede percibir una urgencia subjetiva, estableciendo una experiencia de angustia para cada sujeto. El objetivo de esta investigación fue investigar las posibilidades de una clínica de urgencia subjetiva en el contexto de un hospital universitario en Salvador (Brasil), considerando las experiencias en una Residencia Multiprofesional. Se trata de una investigación cualitativa, de carácter exploratorio, en la que se realizó una revisión teórica sobre el tema y construcción de un caso clínico, con orientación psicoanalítica. La elección del caso se basó en la escucha clínica a lo largo de las sesiones y actuación en un equipo multidisciplinar, considerando los impasses para el tratamiento a largo plazo. Se utilizaron registros documentales elaborados por el psicólogo residente, durante las atenciones, que se realizaron durante tres meses. Los resultados apuntan a las contribuciones de la escucha psicoanalítica en el tratamiento de urgencias y en la actuación de un equipo multidisciplinario en el contexto hospitalario. La subjetivación de la urgencia permitió, en el caso en cuestión, un tratamiento a través de la palabra de lo que había afectado directamente al cuerpo como fenómeno. Se concluye que es relevante discutir el tema de la urgencia y plantear nuevas investigaciones, reintroduciendo el tema de la subjetividad en el contexto hospitalario.(AU)

ABSTRACT

La publicación describe las medidas de bioseguridad y biocustodia para la protección de la salud del personal de los laboratorios, áreas de apoyo, áreas de experimentación animal y bioterios mediante medidas y recomendaciones en bioseguridad y biocustodia

ABSTRACT

La disponibilidad de cepas bacteriana para el estudio de la resistencia bacteriana es clave para los avances en la investigación básica y clínica respecto del tema. Existen pocos biorrepositorios o bancos de bacterias con mecanismos de resistencia conocidos, aisladas de infecciones clínicamente significativas. Una revisión de la literatura revela que sólo en los Estados Unidos de América existe un biobanco de aislados resistentes disponibles para estudios. En esta publicación se cuenta cómo se creó el primer biorrepositorio de bacterias resistentes en Chile asociados a la Red de Laboratorios MICROB-R, con la participación de 11 centros distribuidos a lo largo del país, que a la fecha cuenta con más de 3.500 aislados bacterianos estudiados fenotípica y genotípicamente, disponibles para la comunidad científica chilena.

The availability of bacterial strains for the study of bacterial resistance is key to advances in basic and clinical research. There are few biobanks of bacteria with known resistance mechanisms, isolated from clinically significant infections. A review of the literature reveals that only in the United States of America is there a biobank of resistant isolates. This publication shows the creation of the first biorepository of resistant bacteria Chile associated with the MICROB-R Laboratory Network, with the participation of 11 centers distributed throughout the country, which to date has more than 3,500 bacterial isolates studied phenotypically and genotypically, available to the Chilean scientific community.

ABSTRACT

Com o fim de propiciar um ambiente que possibilite decisões ágeis, criativas e efetivas no sistema de saúde, o Laboratório de Inovações no SUS do Ceará (FeliciLab) tem adotado o uso de métodos inovadores de gestão de projetos. Este relato de experiência tem como objetivo apresentar como a Escola de Saúde Pública do Ceará (ESP/CE) conseguiu, através do FeliciLab, orquestrar um conjunto de pessoas, métodos e tecnologias capazes de provocar uma quebra em padrões até então apresentados no mercado de tecnologias direcionadas para o sistema de saúde, diante da crise sanitária ocasionada pela pandemia de covid-19. Com adoção de metodologias mais ágeis de condução de trabalho e gerenciamento de crises, ampliou-se a percepção de valor da tecnologia da informação (TI) da ESP/CE, nutrida pela compreensão de que inovar é mais do que produzir e implantar tecnologias: trata-se de transformar os modos de criar e consumir soluções, de forma a impactar os contextos e as experiências das pessoas.

In order to provide an environment with agile, creative, and effective decisions in the health system, the Felicilab laboratory has been adopting the use of innovative project management methods. This experience report aims to present how the Escola de Saúde Pública do Ceará (ESP/CE), Brazil, had managed to orchestrate, through FeliciLab, a set of people, methods, and technologies from a disruptive perspective of standards hitherto presented in the market of technologies directed to the health system, in face of the health crisis caused by the covid-19 pandemic. With the adoption of more agile methodologies for conducting work and managing crisis, the perception of the value of the Information technology (IT) used at ESP/CE was expanded, understanding that innovating is more than producing and implementing technologies: it means transforming the ways of creating and consume solutions in order to impact on personal experiences and their context.

A fin de propiciar un ambiente con decisiones ágiles, creativas y efectivas en el sistema de salud, el Laboratório de Inovações no SUS do Ceará (FeliciLab) tiene adoptado el uso de métodos innovadores de gestión de proyectos. Este relato de experiencia tiene como objetivo presentar cómo la Escola de Saúde Pública do Ceará (ESP/CE), a través del FeliciLab, ha conseguido orquestar un conjunto de personas,nmétodos y tecnologías capaces para quebrar estándares hasta entonces presentados en el mercado de tecnologías dirigidas al sistema de salud, frente a la crisis de salud provocada por la pandemia de Covid-19. Con la adopción de metodologías más ágiles para la realización del trabajo y la gestión de crisis, se amplió la percepción del valor de la tecnología de la información (TI) ESP/CE, alimentada por el entendimiento de que innovar es más que producir e implementar tecnologías: se trata de transformar formas de crear y consumir soluciones para impactar los contextos y las experiencias de las personas.