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1.
Article in Chinese | WPRIM | ID: wpr-939614

ABSTRACT

The count and recognition of white blood cells in blood smear images play an important role in the diagnosis of blood diseases including leukemia. Traditional manual test results are easily disturbed by many factors. It is necessary to develop an automatic leukocyte analysis system to provide doctors with auxiliary diagnosis, and blood leukocyte segmentation is the basis of automatic analysis. In this paper, we improved the U-Net model and proposed a segmentation algorithm of leukocyte image based on dual path and atrous spatial pyramid pooling. Firstly, the dual path network was introduced into the feature encoder to extract multi-scale leukocyte features, and the atrous spatial pyramid pooling was used to enhance the feature extraction ability of the network. Then the feature decoder composed of convolution and deconvolution was used to restore the segmented target to the original image size to realize the pixel level segmentation of blood leukocytes. Finally, qualitative and quantitative experiments were carried out on three leukocyte data sets to verify the effectiveness of the algorithm. The results showed that compared with other representative algorithms, the proposed blood leukocyte segmentation algorithm had better segmentation results, and the mIoU value could reach more than 0.97. It is hoped that the method could be conducive to the automatic auxiliary diagnosis of blood diseases in the future.


Subject(s)
Algorithms , Leukocytes
2.
Article in Chinese | WPRIM | ID: wpr-928673

ABSTRACT

OBJECTIVE@#To analyze the expression and clinical characteristics of CD68 in bone marrow and peripheral blood of patients with acute myeloid leukemia (AML).@*METHODS@#The expression of CD68 in bone marrow blast cells was detected by four-color flow cytometry in 50 newly diagnosed AML patients and 23 controls. The expression of CD68 in peripheral blood of 85 newly diagnosed AML patients, 29 remission AML patients and 24 controls was detected by ELISA. The correlation between the expression rate of non-M3 AML bone marrow CD68, peripheral blood CD68 concentration and white blood cell count and other clinical data was compared respectively.@*RESULTS@#The median CD68 expression rate in myeloid leukemia cells of non-M3 AML patients was 19.7%, significantly higher than control (0.2%) (P<0.001). The median concentration of non-M3 CD68 in peripheral blood was 67.97 pg/ml, significantly higher than in control (29.94 pg/ml)(P<0.01). There was no statistically significant difference in the plasma CD68 concentration of the peripheral blood between the newly diagnosed (45.72 pg/ml) and the remission stage (55.12 pg/ml) of non-M3 AML patients by paired analysis (P>0.05). The results showed that the higher the expression rate of CD68 in bone marrow, the higher the count of white blood cells in peripheral blood, and the lower the count of hemoglobin and platelet in peripheral blood. The higher the plasma concentration of CD68 in peripheral blood, the higher the white blood cell count and the lower the complete remission rate.@*CONCLUSION@#The expression of CD68 both in bone marrow and peripheral blood of patients with non-M3 AML is higher than that of control group. Patients with high expression of CD68 show a low rate of complete remission, suggesting that the expression level of CD68 is correlated with treatment response.


Subject(s)
Bone Marrow , Flow Cytometry , Humans , Leukemia, Myeloid, Acute , Leukocytes , Prognosis , Remission Induction
3.
Rev. odontol. UNESP (Online) ; 51: e20220004, 2022. ilus
Article in English | LILACS, BBO | ID: biblio-1389706

ABSTRACT

Introduction: Platelet-rich fibrin (PRF) is formed by an autologous blood concentrate, with properties that promote cell proliferation and regenerationof bone, gingival and epithelial tissue. Objective: To compare four different procedures for processing as well as obtaining PRF, and analyzing their formation through laboratory techniques. The purpose of the study is to validate a method that produces higher quality PRF for oral surgery use in different branches of dentistry. Material and method: The experiment consisted of collecting blood from 12 volunteers, and processing each patient's sample in 4 different ways. In the following two-stage process analysis, the first, quantitative, step was to analyze the samples' platelet-poor plasma (PPP),with a Neubauer Hemocytometer to count blood components. In the second, qualitative step, the sample's PRF were analyzed under microscopy using histological slides. Result: The statistical analysis of the leukocyte, red blood cell and platelet count did not show any significant distinction when comparing different processes. Histological analysis of the PRF showed fibrin network with platelet aggregation, several leukocytes and presence of red blood cells, with double centrifuged samples presenting more white blood cells than the others. Conclusion: Among the analyzed procedures, the sample centrifuged once at 3000 RPM (1900 G) for 10 min showed the best quality PRF.


Introdução: A fibrina rica em plaquetas (FRP) é formada por um concentrado sanguíneo autólogo, com propriedades de promover a proliferação celular, regeneração de tecido, dentre eles tecido ósseo, gengival e epitelial. Objetivo: Comparar quatro tipos diferentes de processamento e obtenção de FRP, analisando a sua formação através de técnicas laboratoriais. A finalidade do estudo é validar um método que obtenha maior qualidade para o uso em cirurgias nas diversas áreas da odontologia. Material e método: O experimento foi constituído de uma coleta de sangue de 12 voluntários, onde a amostra de cada paciente foi processada de 4 formas. A análise dos processamentos se deu em duas etapas, onde na primeira, quantitativa, foi analisado o plasma pobre em plaquetas (PPP), através de contagem de constituintes sanguíneos em câmara de Neubauer. Na segunda etapa, qualitativa, foram analisadas as FRP em microscopia através de lâminas histológicas. Resultado: A análise estatística da contagem de leucócitos, hemácias e plaquetas não mostraram diferença significativa entre os distintos processamentos. As análises histológicas do FRP mostraram redes de fibrina com agregação plaquetária, inúmeros leucócitos e presença de hemácias, sendo que as amostras de dupla centrifugação apresentaram mais leucócitos que nas demais. Conclusão: Dos procedimentos analisados, a amostra centrifugada a 1 única vez a 3000 RPM (1900 G) por 10 min mostrou-se a melhor qualidade de FRP.


Subject(s)
Sampling Studies , Statistics, Nonparametric , Dentistry , Platelet-Rich Fibrin , Leukocytes
4.
Prensa méd. argent ; 108(4): 223-227, 20220600. tab
Article in English | LILACS, BINACIS | ID: biblio-1381891

ABSTRACT

Dado que el SARS-CoV-2 causa alteraciones en los parámetros hematológicos, muchos estudios se han concentrado en esta área y los han correlacionado con la gravedad de la enfermedad. Se revisó en un estudio retrospectivo de casos graves de COVID-19, en el cual se contabilizaron y documentaron los datos de hemoglobina (Hb) y glóbulos blancos (WBC) de pacientes reclutados en el período de marzo a julio de 2021. Retrospectivamente, los datos extraídos fueron sobre el nivel de Hb y los recuentos de glóbulos blancos de los informes de laboratorio tanto al ingreso como cuando alta de los pacientes. Se calcularon las frecuencias de anemia o eritrocitopenia (Hb <12 g/dL) y leucocitosis (WBC >11×103/µL) en la población estudiada. De 3637 casos de SARS-COV-2, 250 pacientes con condiciones graves de COVID-19 según la definición de la OMS, ingresaron en la UCI o fallecieron, De 250, 80 (32%) ingresaron a UCI, y el resto 170 casos (68%) fallecieron. Mientras que la edad media de los pacientes fue de 55,46±17,49 años, los pacientes con la condición grave tuvieron significativamente mayor edad que aquellos con la condición leve-moderada (edad media de 50,68 frente a 68,59; P: <0,01). El número medio de glóbulos blancos (WBC) fue de 8,88±7,29 x109/L en todos los pacientes con SARS-COV-2, que es significativamente mayor en los casos graves en comparación con aquellos con enfermedad leve-moderada (10,56 frente a 8,95; P: <0,01). El nivel de Hb (<12 g/dL) fue más bajo en el COVID-19 grave que en otros grupos; sin embargo, no fue estadísticamente significativo. El aumento del número de glóbulos blancos y la disminución de la Hb durante la hospitalización de pacientes con SARS-COV-2, puede predecir un mal resultado. Llegamos a la conclusión de que no sólo el número medio de glóbulos blancos era significativamente mayor en los casos severos también la leucocitosis fue un hallazgo común; lo que indica que un mayor número de glóbulos blancos probablemente puede predecir un mal resultado. Asimismo, el nivel de La Hb fue mayor en el grupo leve-moderado; sin embargo, no fue estadísticamente significativo.


SARS-CoV-2 cause alterations in the hematological parameters and many studies have concentrated on this area and correlated these with severity of disease. A retrospective study of severe cases of COVID-19 were reviewed. The data of hemoglobin (Hb) and white blood cells (WBCs) were accounted and documented. Patients recruited at period from March to July, 2021. Retrospectively, data extracted on the Hb level and WBC counts from Lab reports both at admission and when patients discharge. The frequencies of anemia or erythrocytopenia (Hb <12 g/dL) and leukocytosis (WBC >11×103/µL) was calculated in the studied population. Of 3637 SARS-COV-2 cases, 250 patients with severe conditions of COVID-19 as de ne by WHO, which were either admitted to ICU or died. Of 250, 80(32%) were entered to ICU, and the rest 170 cases (68%) who died. While the mean age of the patients was 55.46±17.49 years, patients with the severe condition were signi cantly older than those with the mildmoderate condition (mean age of 50.68 vs. 68.59; P: <0.01). The mean number of white blood cells (WBC) was 8.88±7.29 x109/L in all SARS-COV-2 patients, which is signi cantly higher in the severe cases compared to those with the mild-moderate disease (10.56 vs. 8.95; P: <0.01). The Hb level (<12 g/ dL) was lower in the severe COVID-19 than other groups; however, it was not statistically signi cant. Increased number of WBCs and dropped Hb level during hospitalization of SARS-COV-2 patients may predict a poor outcome. We concluded that not only the mean number of WBCs was signi cantly higher in the severe cases also leukocytosis was a common nding; indicating that an increased number of WBCs may probably predict a poor outcome. Also, the level of Hb was higher in the mild-moderate group; however, it was not statistically signi cant.


Subject(s)
Humans , Adolescent , Adult , Middle Aged , Hemoglobins/analysis , Clinical Laboratory Techniques/methods , Patient Acuity , SARS-CoV-2/immunology , COVID-19/immunology , Leukocytes
5.
Article in Chinese | WPRIM | ID: wpr-936111

ABSTRACT

OBJECTIVE@#To compare the clinical efficacy of initial periodontal therapy in periodontitis patients with or without type 2 diabetes mellitus and its correlation with white blood cell counts.@*METHODS@#In this study, 32 chronic periodontitis patients without systemic disease (CP group) and 27 chronic periodontitis patients with type 2 diabetes mellitus (CP+DM group) were enrolled. At admission, all the patients went through periodontal examination and fasting blood examination(baseline). Probing depth (PD), attachment loss (AL), bleeding index (BI), plaque index (PLI), white blood cells (WBC) counts and fasting blood glucose (FBG) were recorded respectively, while hemoglobin A1c (HbA1c) was recorded only in CP+DM group. After that, initial periodontal therapy was performed. All the tests were repeated 3 and 6 months after treatment. The changes of periodontal clinical indexes and WBC levels were compared between the two groups before and after treatment, and the correlation between WBC and periodontal clinical indexes and glucose metabolism indexes were analyzed by generalized linear mixed model.@*RESULTS@#At baseline, the periodontal inflammation and destruction were similar in CP and CP+DM group, but the WBC level was significantly higher in CP+DM groups [(6.01±1.26)×109/L vs. (7.14±1.99)×109/L, P=0.01]. After 3 and 6 months of initial periodontal therapy, the mean PD, AL, BI, and PLI in CP+DM and CP groups were significantly lower than the baseline, and the PD in CP+DM group was further decreased by 6 months compared with 3 months [(3.33±0.62) mm vs. (3.61±0.60) mm, P < 0.05]. However, none of these periodontal indexes showed significant difference between the two groups by 3 or 6 months. In CP+DM group, HbA1c at 3 months and 6 months were significantly lower than the baseline [(7.09±0.79)% vs. (7.64±1.16)%, P < 0.05; (7.06±0.78)% vs. (7.64±1.16)%, P < 0.05], and FBG was significantly lower than the baseline by 6 months [(7.35±1.14) mmol/L vs. (8.40±1.43) mmol/L, P < 0.05]. The WBC level in CP group was significantly lower than the baseline level by 3 months [(5.35±1.37)×109/L vs. (6.01±1.26)×109/L, P < 0.05], while that in CP+DM group was significantly lower than the baseline level by 6 months [(6.00±1.37)×109/L vs. (7.14±1.99)×109/L, P < 0.05]. The analysis of genera-lized linear mixed model showed that WBC level was significantly positively correlated with PD and FBG (P < 0.05).@*CONCLUSION@#Initial periodontal therapy can effectively improve the periodontal clinical status of patients with or without type 2 diabetes mellitus, and have benefits on glycemic control in diabetic patients. However, the response of periodontal indexes and WBC level to initial therapy were relatively delayed in diabetic patients. WBC plays an important role in the correlation between diabetes mellitus and periodontitis.


Subject(s)
Chronic Periodontitis/therapy , Diabetes Mellitus, Type 2/complications , Glycated Hemoglobin A/analysis , Humans , Leukocytes/chemistry , Periodontal Index
6.
Hematol., Transfus. Cell Ther. (Impr.) ; 43(4): 494-498, Oct.-Dec. 2021. ilus
Article in English | LILACS | ID: biblio-1350801

ABSTRACT

ABSTRACT Introduction: Peripheral blood leukocytes are a suitable cell model for science research. However, blood samples from healthy volunteers are limited in volume and difficult to obtain due to the complexity of volunteer recruitment. Objective: Therefore, it is urgent to find an alternative source of peripheral blood leukocytes. Method: One of the possibilities is the use of leukocyte reduction filters (LRFs) in blood banks that is used for preparation of leukoreduced blood products. More than 90% of the leukocytes are trapped in the leukofilters allowing the desired blood product to pass through. Results: It has been reported that the biological function of leukocytes collected from the filters are no different from those isolated from buffy coats, leukapheresis products and whole blood (WB) cells. Moreover, LRFs are waste products that are discarded after leukoreduction. Conclusion: Thus, leukofilters represent an economic source of human cell populations that can be used for a variety of investigative purposes, with no cost. In the present study, we reviewed the different usage of LRFs in the research, clinical and commercial applications.


Subject(s)
Leukocyte Reduction Procedures , Leukocytes
7.
Arq. Asma, Alerg. Imunol ; 5(4): 361-370, out.dez.2021. ilus
Article in English, Portuguese | LILACS | ID: biblio-1399785

ABSTRACT

O corpo humano tende sempre a procurar um estado de homeostase, buscando o equilíbrio entre todos os sistemas. O exercício físico está presente na rotina diária de indivíduos, mesmo com objetivos diferentes, porém a influência no sistema imunológico não é muitas vezes abordada como fator relevante. O sistema imune é responsável por proteger o organismo contra infecções e doenças, podendo ser modulado perante a resposta de exercícios físicos regulares. Tendo em vista que, atualmente, existe uma preocupação maior em tornar e manter a imunidade eficiente, a prática regular e moderada do exercício pode contribuir para uma maior eficácia desse sistema, dessa forma, podendo ser considerada uma proteção ao corpo humano. O objetivo dessa revisão foi sintetizar os dados de estudos presentes na literatura que demonstram a influência do exercício físico na resposta do sistema imunológico, tornando possível compreender as alterações moleculares, fisiológicas, metabólicas e celulares que levam a um tipo específico de resposta do organismo humano.


The human body always tends to seek a homeostasis state, trying to balance all systems. Physical exercise is present in the routine of individuals even with different goals, but the influence in the immune system isnt a relevant factor. The immune system is responsible for protecting the human body against some infections and diseases, and could be modulated in response by some regular physical exercise. At the moment there is a greater concern to keep efficient immunity, a practice of regular and moderate exercise can contribute to a better effectiveness of this system, thus, it can be considered a form of protection to the human body. The objective of this review was to synthesize some data from any studies presented in the literature that demonstrate the influence of physical exercise on the immune system response. Making it possible to understand the molecular mechanisms, physiological, metabolic and cellular changes that turn to a specific type of response in the human body.


Subject(s)
Humans , Exercise , Immune System , Immunity , Dendritic Cells , Immunoglobulins , Killer Cells, Natural , Lymphocytes , Monocytes , Cytokines , Human Body , Chemokines , Protective Factors , Endurance Training , Homeostasis , Leukocytes , Macrophages , Neutrophils
8.
Electron. j. biotechnol ; 52: 13-20, July. 2021. tab, graf, ilus
Article in English | LILACS | ID: biblio-1283173

ABSTRACT

BACKGROUND: In fish farming, the plant extracts containing antioxidant compounds have been added to the diet for enhancing pathogen resistance. In vitro studies evaluating the antioxidant effect of herbal extracts on fish cell models have focused on ROS production and the respiratory burst mechanism. However, the effects on enzymatic antioxidant defense on salmon leukocytes have not been evaluated. This study aims to evaluate the enzymatic antioxidant defense and ROS-induced cell damage in Salmon Head Kidney-1 (SHK-1) cell line exposed to polyphenol-enriched extract from Sambucus nigra flowers. RESULTS: Firstly, the Total Reactive Antioxidant Power (TRAP) assay of elderflower polyphenol (EP) was evaluated, showing 459 and 489 times more active than gallic acid and butyl hydroxy toluene (BHT), respectively. The toxic effect of EP on salmon cells was not significant at concentrations below 120 mg/ mL and no hemolysis activity was observed between 20 and 400 mg/mL. The treatment of SHK-1 cell line with EP decreased both the lipid peroxidation and protein oxidation induced by H2O2, which could be associated with decreasing oxidative stress in the SHK-1 cells since the GSH/GSSG ratio increased when only EP was added. CONCLUSIONS: These results suggest that plant extracts enriched with polyphenols could improve the enzymatic antioxidant defense of salmon leukocytes and protect the cells against ROS-induced cell damage


Subject(s)
Salmon , Plant Extracts/pharmacology , Sambucus nigra/chemistry , Polyphenols/pharmacology , Lipid Peroxidation , Free Radical Scavengers , Reactive Oxygen Species , Aquaculture , Oxidative Stress , Salmo salar , Disease Resistance , Leukocytes , Antioxidants
10.
Journal of Experimental Hematology ; (6): 1312-1317, 2021.
Article in Chinese | WPRIM | ID: wpr-888558

ABSTRACT

OBJECTIVE@#To observe the effect of astaxanthin (ASTA) on the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in suspended leukocyte-depleted red blood cells stored for transfusion.@*METHODS@#The suspended leukocyte-depleted red blood cells were randomly divided into group A, B, C and D. The ASTA was added into preservation solution of suspended leukocyte-depleted red blood cells of group B, C and D with the final concentration 5, 10 and 20 μmol/L, respectively, while DMSO was added into cells of group A in the same volume. After 7, 14, 28 and 42 days of storage, the reactive oxygen species (ROS) content in red blood cells was detected by fluorescence microplate reader, malondialdehyde (MDA) content was detected by thiobarbituric acid (TBA) method, activity of SOD was detected by xanthine oxidase method, the activity of CAT was detected by visible light method, and activity of GSH-Px was detected by colorimetry.@*RESULTS@#After 7, 14, 28 and 42 days of storage, the contents of ROS and MDA in suspended red blood cells of group B, C and D were significantly lower(P<0.05), while the activities of SOD and GSH-Px were higher than those of group A(P<0.05); and CAT activity in cells treated by ASTA was significantly higher at 28 and 42 days of storage in comparison with that of group A(P<0.05). There were positive correlations between the ROS, MDA content in suspended red blood cells of group A, B, C, D and storage time(P<0.01), while negative correlation between SOD, CAT, GSH-Px activity and storage time(P<0.01).@*CONCLUSION@#ASTA can decrease the oxidative stress level and peroxide damage degree by increasing the antioxidant enzyme activities in suspended leukocyte-depleted red blood cells during storage.


Subject(s)
Antioxidants , Catalase/metabolism , Erythrocytes , Leukocytes , Oxidative Stress , Superoxide Dismutase/metabolism , Xanthophylls
11.
Article in Chinese | WPRIM | ID: wpr-880080

ABSTRACT

OBJECTIVE@#To detect the relationship between leukocytes derived microparticle (CD45@*METHODS@#The expression of CD45@*RESULTS@#The percentages of CD45@*CONCLUSION@#High level of CD45


Subject(s)
Flow Cytometry , Humans , Leukemia, Myeloid, Acute , Leukocytes , Neoplasm, Residual , Prognosis
12.
Article in English | WPRIM | ID: wpr-878317

ABSTRACT

Objective@#In the present study, the ABCA1 was used as a label to capture specific exosomes, the level of ABCA1-labeled exosomal microRNA-135a (miR-135a) was evaluated for the diagnosis of Alzheimer's disease (AD), especially in patients with early stages of AD.@*Methods@#This is a preliminary research focused on the levels of ABCA1 in WBCs, RBCs, HT-22 cells, and neuron cells. The diagnostic value of ABCA1-labeled exosomal miR-135a was examined using the CSF and serum of APP/PS1 double transgenic mice, and 152 patients with SCD, 131 patients with MCI, 198 patients with DAT, and 30 control subjects.@*Results@#The level of ABCA1 exosomes harvested from HT-22 cells and neuron culture medium was significantly higher compared to that of RBCs and WBCs ( @*Conclusion@#This study outlines a method to capture specific exosomes and detect them using immunological methods, which is more efficient for early diagnosis of AD.


Subject(s)
ATP Binding Cassette Transporter 1/cerebrospinal fluid , Aged , Aged, 80 and over , Alzheimer Disease/cerebrospinal fluid , Animals , Biomarkers/cerebrospinal fluid , Cell Line , Cognitive Dysfunction/cerebrospinal fluid , Erythrocytes/metabolism , Exosomes , Female , Humans , Leukocytes/metabolism , Male , Mice, Transgenic , MicroRNAs/blood , Neurons/metabolism
13.
Pesqui. vet. bras ; 41: e06688, 2021. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1279535

ABSTRACT

This study aimed at performing cytometric phenotyping of the blood samples from free-living, young white-eyed parakeets (Psittacara leucophthalmus), stained with 3,3-dihexyloxacarbocyanine [DiOC6(3)]. DiOC6(3)-stained whole blood samples from 19 free-living, young white-eyed parakeets were analyzed by flow cytometry and cell types were distinguished by their typical fluorescence in blue laser channel (FL-1) and SSC (side scatter). It was possible to differentiate erythrocytes (58.3±13.6) from leukocytes (32.4±13.1) and some of the leucocyte subpopulations: lymphocytes/thrombocytes (29.7±7.7), monocytes (30.6±8.5), and granulocytes (5.9-26). However, lymphocytes and thrombocytes could not be sorted in the plots. Our study determined that the predominant population in white-eyed parakeet (P. leucophthalmus) was lymphocytes, thrombocytes, and monocytes in the leucocytes gates in comparison to the granulocyte population. The cytometry method and use of DiOC6(3) stain was available for parakeets blood samples and can be studied and applied to other species of parrots.(AU)


Este estudo teve como objetivo realizar a fenotipagem citométrica com 3,3-di-hexiloxacarbocianina [DiOC6 (3)] de amostras de sangue de maritacas jovens de vida-livre (Psittacara leucophthalmus). As amostras de sangue total, coradas com DiOC6(3) de 19 maritacas de vida livre, foram analisadas por citometria de fluxo e os tipos de células foram distinguidos por sua fluorescência típica no canal laser azul (FL-1) e SSC (dispersão lateral). Foi possível diferenciar eritrócitos (58,3±13,6) de leucócitos (32,4±13,1) e algumas subpopulações de leucócitos: linfócitos/trombócitos (29,7±7,7), monócitos (30,6±8,5) e granulócitos (5,9-26), entretanto, linfócitos e trombócitos não puderam ser diferenciados em duas populações distintas. Nosso estudo determinou que a população predominante P. leucophthalmus foi mononuclear agranulocítica em comparação com a taxa de aquisição da população granulocítica. A metodologia de citometria de fluxo com uso da coloração de DiOC6(3) foi aplicável a amostras sanguíneas das maritacas e pode ser estudado e aplicado para outras espécies de psitacídeos.(AU)


Subject(s)
Animals , Parakeets , Parrots/blood , Flow Cytometry , Leukocytes , Phenotype
14.
Arq. gastroenterol ; 57(4): 366-374, Oct.-Dec. 2020. tab, graf
Article in English | LILACS | ID: biblio-1142336

ABSTRACT

ABSTRACT BACKGROUND: During the Helicobacter pylori (HP) infection, the infiltration of the leukocytes into stomach mucosa is directed by locally produced chemokines that play a decisive role in infection outcome. The CagA is the most potent virulence factor of HP, so that the infection with CagA + strains is associated with more severe complications than infection with CagA - HP. OBJECTIVE: The aim was to determine the expression of chemokines CXCL10, CCL17, CCL20 and CCL22, and their receptors by CagA + HP- and CagA - HP-derived crude extract (HP-CE)-stimulated peripheral blood mononuclear cells (PBMCs) from peptic ulcer (PU) patients. METHODS: The serum and the PBMCs were collected from 20 HP-infected PU patients, 20 HP-infected asymptomatic subjects (HIA) and 20 non-infected healthy subjects (NHS). The PBMCs were cultured in absence of stimulator or with 10 µg CagA + HP crude extract (CagA + CE), 10 µg CagA - HP crude extract (CagA - CE). Chemokines and receptors were measured by ELISA and real time-PCR respectively. RESULTS: In PU patients, the production of chemokines CXCL10, CCL17, CCL20 and CCL22, and the expression of chemokine receptors CXCR3, CCR4 and CCR6 by CagA + CE-induced PBMCs were significantly higher than non-stimulated and CagA - CE stimulated cultures. The CXCL10 production by CagA + CE stimulated PBMCs from HIA subjects was significantly higher than the equal cultures from PU and NHS groups. The CCL17 and the CCL20 production by non-stimulated, CagA + CE stimulated, and CagA - CE stimulated PBMCs from PU subjects were significantly higher than the equal cultures from NHS and HIA groups. The CCL22 production by non-stimulated, CagA + CE stimulated and CagA - CE stimulated PBMCs from NHS group were significantly higher than the equal cultures from HIA and PU groups. The CagA + CE stimulated PBMCs from HIA subjects expressed lower amounts of CCR6 in comparison with CagA + CE stimulated PBMCs from NHS and PU groups. The serum levels CXCL10 and CCL20 in PU and HIA groups were significantly higher than NHS subjects. NHS and HIA groups displayed higher serum levels of CCL22 in comparison with PU patients. CONCLUSION: Results indicated that the CagA status of bacterium influence the expression of chemokines and receptors by HP-CE stimulated PBMCs from PU patients.


RESUMO CONTEXTO: Durante a infecção por Helicobacter pylori (HP), a infiltração dos leucócitos na mucosa estomacal é dirigida por quimiocinas produzidas localmente que desempenham um papel decisivo no resultado da infecção. O CagA é o fator de virulência mais potente do HP, de modo que a infecção com cepas CagA + está associada a complicações mais graves do que a infecção com CagA - HP. OBJETIVO: O objetivo foi determinar a expressão das quimiocinas CXCL10, CCL17, CCL20 e CCL22, e seus receptores por CagA + HP- e CagA - extrato bruto (EB) derivado de HP (HP-EB) de células mononucleares do sangue periférico (CMSP) de pacientes com úlcera péptica (UP). MÉTODOS: O soro e as CMSP foram coletados de 20 pacientes com UP infectados pelo HP, 20 indivíduos assintomáticos infectados pelo HP (AI-HP) e 20 indivíduos saudáveis não infectados pelo HP (NI-HP). As CMSP foram cultivadas na ausência de estimulador ou com extrato bruto CagA + HP de 10 μg (CagA + EB), 10 μg CagA - extrato bruto HP (CagA - EB). Quimiocinas e receptores foram medidos por ELISA e PCR em tempo real, respectivamente. RESULTADOS: Em pacientes com UP a produção de quimiocinas CXCL10, CCL17, CCL20 e CCL22, e a expressão dos receptores de quimiocina CXCR3, CCR4 e CCR6 por CagA + CMSP induzidos pelo EB foram significativamente maiores do que as culturas não estimuladas e CagA - EB estimulados. A produção de CXCL10 por CagA + EB estimulou as CMSP de sujeitos AI-HP em proporção significativamente maior do que as culturas iguais dos grupos UP e NI-HP. A produção de CCL17 e CCL20 por grupos não estimulados, CagA + EB estimulado, e CagA - EB estimulou CMSP de sujeitos com UP e foram significativamente superiores às culturas iguais dos grupos NI-HP e AI-HP. A produção de CCL22 por grupos não estimulados, CagA + EB estimulado e CagA - EB estimulado pelo grupo NI-HP foram significativamente maiores do que as culturas iguais dos grupos AI-HP e PU. O CagA + EB estimulou as CMSP dos sujeitos do AI-HP, expressando menores quantidades de CCR6 em comparação com as CMSP estimuladas pelo CagA + EB de grupos NI-HP e UP. Os níveis sanguíneos de CXCL10 e CCL20 nos grupos UP e AI-HP foram significativamente superiores aos dos sujeitos do NI-HP. Os grupos NI-HP e AI-HP apresentaram níveis sanguíneos mais elevados de CCL22 em comparação com pacientes com UP. CONCLUSÃO: Os resultados indicaram que o estado CagA da bactéria influencia a expressão de quimiocinas e receptores por HP-EB estimulados CMSP de pacientes com UP.


Subject(s)
Humans , Peptic Ulcer , Helicobacter pylori , Bacterial Proteins , Leukocytes, Mononuclear , Helicobacter Infections , Virulence Factors , Chemokine CCL17 , Chemokine CCL20 , Chemokine CCL22 , Leukocytes , Antigens, Bacterial
15.
Rev. chil. neuro-psiquiatr ; 58(3): 221-229, set. 2020. tab, graf
Article in Spanish | LILACS | ID: biblio-1138576

ABSTRACT

Resumen Introducción: La masticación emerge como un mecanismo fisiológico que modula diversos procesos encefálicos aún en proceso de entendimiento, entre dichos procesos se encuentra el estrés. El objetivo fue determinar la influencia del tipo de masticación sobre el estrés crónico en los niveles leucocitarios. Material y método: Se empleó 48 ratones machos de la cepa Balb/c que se dividieron en 4 grupos iguales: grupo N (masticación normal), grupo NE (Masticación normal + estrés), grupo D (masticación deficiente) y grupo DE (Masticación deficiente + estrés). Los niveles leucocitarios fueron evaluados a la cuarta y a la décima segunda semana de iniciado el experimento. Se sacrificó a cada ratón para obtener su muestra de sangre y evaluar su leucograma. Resultados: Se encontró, a la cuarta semana, linfocitopenia del grupo DE respecto al grupo D y al grupo N (p<0,05), y neutrofilia en el grupo DE respecto al grupo D (p<0,05). A la décima segunda semana se observó linfocitopenia y neutrofilia de los grupos DE y NE al compararlos con los grupos D y N (p<0,05), respectivamente. Conclusión: el estrés crónico produce linfocitopenia y neutrofilia, independiente del tipo de masticación.


Introduction: Chewing emerges as a physiological mechanism that modulates various processes in the brain, even in the process of understanding, among these processes is stress. The objective was to determine the influence of the type of chewing on chronic stress in leukocyte levels. Material and method: 48 male Balb/c mice were used that were divided into 4 equal groups: group N (normal chewing), group NE (normal chewing + stress), group D (poor chewing) and group DE (poor chewing + stress). The leukocyte levels were evaluated at the fourth and the twelfth week of beginning the experiment. Each mouse was sacrificed to obtain the blood sample and evaluate its leukogram. Results: At the fourth week, lymphocytopenia of the DE group regarding group D and group N (p <0.05), and neutrophilia were found in the DE group regarding group D (p <0.05). At the twelfth week, lymphocytopenia and neutrophilia of the DE and NE groups were observed when compared with groups D and N (p <0.05), respectively. Conclusion: Chronic stress produces lymphocytopenia and neutrophilia, regardless of the type of chewing.


Subject(s)
Animals , Mice , Stress, Physiological , Brain , Leukocytes , Mastication
16.
Prensa méd. argent ; 106(7): 444-450, 20200000. fig
Article in English | LILACS, BINACIS | ID: biblio-1366968

ABSTRACT

Women were studied undergoing ICSI for 84 who suffer non-pregnancy at the Fertility Center, Al-Sadr Medical Hospital in Najaf Governorate, Period between January 2019 and March 2020. WBC, Vitamin D3 and ß-hCG were measured, The pregnant women was divided into (Pregnancy Group, and spontaneous miscarriage) and then demonstrate the immunological effect on pregnancy of women after ICSI technique. Current resultsstudy showed a significant increase (p<0.05) in hormone level ß-hCG is evidence of the presence of high success rates for pregnancy in women who performed operations IVF, where the success rate at the beginning of the matter reached 61.9%, after which it decreased to 33.3% after the first three months due to the occurrence of spontaneous miscarriage of pregnant women due to various immunological and physiological reasons, a positive correlation between the level of ß-hCG and other parameters in the study (Vitamin D3 -WBC).Also The current resultsshowed a significant decrease in a groups (pregnancy failure) and the group (spontaneous miscarriage) compared with the control group (continued pregnancy) in relation to the level of vitamin D3 Also, The current results showed a significant increasein (pregnancy failure) and (spontaneous miscarriage) compared with control groups (continuation of pregnancy) in relation WBC numbers, and the present study founds a negative relationship between the level of vitamin D3 and WBC.


Subject(s)
Humans , Female , Pregnancy/immunology , Abortion, Spontaneous/immunology , Cholecalciferol/deficiency , Sperm Injections, Intracytoplasmic/methods , Chorionic Gonadotropin/immunology , Leukocytes/immunology
17.
Dent. press endod ; 10(2): 67-72, maio-ago.2020. Tab, Ilus
Article in English | LILACS | ID: biblio-1344654

ABSTRACT

ntrodução: A cirurgia parendodôntica é uma alternativa ao tratamento endodôntico convencional, principalmente em casos envolvendo lesões perirradiculares associadas a processos osteolíticos, em que a terapia endodôntica convencional ou seu retratamento não obtiveram sucesso. Novos métodos terapêuticos têm ganhado espaço na clínica odontológica para esse tipo de caso, entre os quais se destaca a fibrina rica em plaquetas e leucócitos, que, ao concentrar e permitir uma liberação mais prolongada dos fatores de crescimento, promove uma modulação do processo reparador. Esse processo é baseado na otimização da regeneração tecidual através da inserção da membrana de fibrina. Objetivo: O objetivo do presente trabalho é apresentar, por meio do relato de um caso clínico, a utilização de fibrina rica em plaquetas e leucócitos em cirurgia parendodôntica, com intuito de reparo ósseo. Resultados: O acompanhamento clínico e tomográfico foi realizado após 190 dias, momento no qual foi constatada ausência de sintomatologia, bem como completo reparo da lesão e restabelecimento do osso cortical vestibular, além de ausência de lesão detectável em tomografia. Conclusão: No caso clínico relatado, houve uma completa regeneração tecidual da área envolvida na lesão (AU).


Introduction: Parendodontic surgery is an alternative to conventional endodontic treatment, especially in cases involving periradicular lesions associated with osteolytic processes, in which conventional endodontic therapy or retreatment was not successful. New therapeutic methods for the treatment of these cases have ground in clinical dentistry . For example, leukocyteand platelet-rich fibrin which has a higher concentration and more prolonged release of growth factors, modulates the repair process. This process is based on the optimization of tissue regeneration through the insertion of fibrin membrane. Objective: The objective of this study is to report a case in which leukocyte- and platelet-rich fibrin associated with parendodontic surgery was used for bone repair. Results: Clinical and tomographic follow-up was performed after 72 days, which revealed absence of any symptoms, complete repair of the lesion, buccal cortical bone regeneration, and absence of detectable lesion on tomography. Conclusion: There was complete tissue regeneration at the lesion site(AU).


Subject(s)
Dental Clinics , Platelet-Rich Fibrin , Regenerative Endodontics , Leukocytes , Apicoectomy , Retreatment , Methods
18.
Infectio ; 24(2): 103-107, abr.-jun. 2020. tab, graf
Article in Spanish | LILACS, COLNAL | ID: biblio-1114849

ABSTRACT

Resumen Objetivo: establecer una metodología predictiva de aplicación clínica de recuentos de CD4+ en rangos de interés clínico a partir del recuento absoluto de leucocitos. Metodología: a partir de los valores secuenciales de leucocitos y linfocitos CD4+ de 9 pacientes, se observaron patrones matemáticos que posteriormente fueron aplicados en un estudio ciego con 71 casos para confirmar su capacidad predictiva, midiendo porcentajes de especificidad y sensibilidad. Resultados: se determinaron cinco patrones matemáticos que predicen en el 99% de los casos los distintos recuentos de CD4+ a partir de recuentos de leucocitos con valores de especificidad y sensibilidad del 99%. Conclusiones: los patrones matemáticos encontrados entre recuento de leucocitos y CD4+ sugieren que este fenómeno prácticamente es determinista.


Abstract Objective: To establish a predictive methodology of CD4+ counts for clinical application in ranges of clinical interest based on the absolute leukocyte count. Methodology: From sequential values of leukocytes and CD4+ lymphocytes of nine patients, mathematical patterns were observed and applied in a blind study with 71 cases to confirm their predictive capacity, measuring percentages of specificity and sensitivity. Results: Five mathematical patterns were determined that predict 99% of the cases in which CD4+ counts are obtained from leukocyte counts with specificity and sensitivity values of 99%. Conclusions: The mathematical patterns found between leukocytes and CD4 counts suggest that this phenomenon is practically deterministic.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Antiretroviral Therapy, Highly Active , CD4 Antigens , Public Health , HIV , Flow Cytometry , Leukocytes
19.
Rev. bras. ciênc. mov ; 28(2): 123-128, abr.-jun. 2020. tab
Article in Portuguese | LILACS | ID: biblio-1127621

ABSTRACT

A obesidade pode influenciar no baixo desempenho da aptidão cardiorrespiratória (APCR), bem como colaborar com alterações nos níveis de indicadores hematológicos. Objetivo: Verificar se existe associação entre indicadores antropométricos, APCR e perfil hematológico de adolescentes no sul do Brasil. Método: Estudo transversal, em que foram avaliados 1 6 98 ado lescentes (742 do sexo masculino) com idade entre 10 e 17 anos, de escolas da rede pública e particular de Santa Cruz do Sul-RS. A avaliação antropométrica foi realizada, utilizando-se os seguintes parâmetros: IMC e circunferência da cintura (CC). Para o teste de APCR, foi utilizado o test e de co rrida/cam inha do s 6 minutos. Para reconhecimento do perfil hematológico, foram avaliados os seguintes parâmetros: leucócitos (WBC), eritrócitos (RBC), hemoglobina (HBC), hematócrito (HCT) e amplitude de distribuição dos glóbulos vermelhos (RDW). A associação entre as variáveis contínuas foi testada por meio da correlação de Pearson. Foram considerados significativos os valores de p<0,05. Resultados: Nos meninos, identificou-se relação fraca entre IMC com RBC (r=0,293; p<0,001) e com HCT (r=0,271; p <0,001 ). A CC também demonstrou associação fraca com estas duas variáveis hematológicas (RBC: r=0,311; p<0,001; HCT: r=0,291; p<0,001). Os níveis de APCR estiveram associados, também de fo rma fraca e direta, com HBC (r=0,224; p<0,001) e HCT (r=0,258; p<0,001). Para o sexo feminino, os níveis de W BC associaram-se, de forma fraca, com IMC (r=0,208; p<0,001) e com CC (r=0,185; <0,001). Conclusão: Os resultados obtidos no presente estudo evidenciam correlação positiva, porém fraca, entre o perfil hematológico dos adolescentes com as variáveis antropométricas e APCR...(AU)


Obesity might cause cardiorespiratory fitness (CRF) to underperform, as well as collaborate with changes in hematological parameters. Aim: To investigate the asso ciation between anthropometric indicators, CRF, and the hematological profiles of adolescents in Southern Brazil. Method: A cross-sectional study of 1,698 adolescents (742 boys, 956 girls), aged between 10 and 17 from p ublic and private schools of Santa Cruz do Sul, State of Rio Grande do Sul, was conducted. Furt h ermore, an anthropometric evaluation of the following parameters was performed: body mass index (BMI) and waist circumference (WC). CRF was evaluated using the 6-minute run/walk test. Hematolo gical p rofile was evaluated based on the following parameters: leucocytes (WBC), erythrocytes (RBC), hemoglobin (HGB), hematocrit (HCT), and red cell distribution width (RDW). The association of continuo us v ariables was tested through Pearson's correlation; p values lower than 0.05 were considered significant. Result s: The boys' BMI presented a weak correlation with RBC (r = 0.293; p < 0.001) and HCT (r = 0.271; p < 0.001). Their WC also presented a weak correlation with both hematological variables (RBC: r = 0.311; p < 0.001; HCT: r = 0.291; p < 0.001). Their CRF levels exhibited a direct but weak asso ciatio n wit h HGB (r = 0.224; p < 0.001) and HCT (r = 0.258; p < 0.001). The WBC levels of girls were fo un d t o be weak ly associated with BMI (r = 0.208; p < 0.001) and WC (r = 0.185; p < 0 .0 01 ). Co nclusio n: Th e result s highlight a positively weak correlation of the hematological profile of adolescents with an throp ometric variables and CRF...(AU)


Subject(s)
Humans , Male , Female , Adolescent , Hemoglobins , Body Mass Index , Erythrocytes , Walk Test , Cardiorespiratory Fitness , Hematocrit , Leukocytes , Obesity , Cross-Sectional Studies , Erythrocyte Indices , Reference Standards , Waist Circumference , Indicators and Reagents
20.
Rev. bras. anal. clin ; 52(1): 77-81, 20200330. graf, ilus
Article in Portuguese | LILACS | ID: biblio-1116499

ABSTRACT

Objetivo: As infecções urinárias (ITU) acometem indivíduos no mundo inteiro e são causadas principalmente por bactérias Gram-negativas, sendo o diagnóstico laboratorial realizado pelo EAS e urocultura. Nesse estudo foram pesquisadas, em amostras de uroculturas positivas, as alterações apresentadas no exame de EAS, principalmente os marcadores de leucócitos e nitrito, e se estes poderiam contribuir para o diagnóstico da ITU. Também foram pesquisadas a faixa etária e a bactéria mais prevalente. Métodos: Um estudo retrospectivo foi realizado com base no levantamento de dados dos exames de urocultura realizados no Laboratório Clínico da PUC Goiás (LAC/PUC Goiás), no período de janeiro/2017 a agosto/2018, de acordo com idade, gênero do paciente e a espécie bacteriana isolada. Resultados: De 629 uroculturas positivas foi encontrada prevalência de ITU em adultos (63,3%), com predomínio no sexo feminino (85,8%), sendo a Escherichia coli (58,9%) a bactéria mais isolada. O nitrito foi detectado em 36,9% e a leucocitúria em 60,6% das amostras. Conclusão: Os resultados evidenciaram predomínio de ITU em adultos do sexo feminino, sendo a Escherichia coli a bactéria com maior prevalência. Quanto aos resultados do EAS, a leucocitúria revelou-se um marcador mais sensível para auxílio diagnóstico de ITU em relação ao nitrito, corroborando com a literatura científica especializada.


Objective: Urinary tract infections (UTI) affect individuals worldwide and are mainly caused by Gram-negative bacteria, and the laboratory diagnosis is performed by urinalysis and uroculture. In this study were investigated in samples of positive urocultures, the alterations presented in the examination of urinalysis, mainly leukocytes and nitrite, and if could contribute to the diagnosis of UTI. The most prevalent age group and bacteria were also investigated. Methods: A retrospective study was carried out based on data collection of uroculture examinations performed at the Clinical Laboratory of PUC Goiás (LAC/PUC Goiás), from january/2017 to august/2018, according to age, gender of patient and the isolated bacterial species. Results: Of 629 positive urocultures, the prevalence of UTI was found in adults (63,3%), with predominance in females (85,8%), and Escherichia coli (58,9%) was the most isolated bacteria. Nitrite was detected in 36,9% and leukocyturia in 60,6% of the samples. Conclusion: The results evidenced a predominance of UTI in female adults, and Escherichia coli was the most prevalent bacteria. Regarding the results of the urinalysis, leukocyturia proved to be a more sensitive marker for the diagnosis aid of UTI in relation to nitrite, corroborating with the literature.


Subject(s)
Humans , Male , Female , Urinary Tract Infections , Urinalysis , Escherichia coli , Leukocytes
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