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Bol. latinoam. Caribe plantas med. aromát ; 20(5): 515-523, sept. 2021. ilus
Article in English | LILACS | ID: biblio-1369061


To explore a new underlying molecular mechanism of Huangkui Extract Powder (HKEP) in the alleviation of diabetic nephropathy (DN). Murine immortalized podocytes were divided into (i) normal glucose (NG, 5.6 mM), (ii) NG + HKEP (0.45 g/L), (iii) HG, and (iv) HG + HKEP (0.45 g/L) groups. MTT assay and flow cytometry were used to detect the podocyte proliferation, apoptosis and cell cycle. Cell viability was inhibited, and apoptosis increased in(iii) HG group compared with (i) NG group (p<0.05). mRNA and protein expression of nephrin and podocin significantly decreased in (iii) HG group compared with (i) NG group (p<0.05). When compared with (iii) HG group, (iv) HG + HKEP group had higher cell viability, lower apoptotic rate and higher mRNA and protein expression of nephrin and podocin (p<0.05). HKEP can attenuate HG-induced podocyte damage, which may be one of the mechanisms of HKEP for attenuating DN.

Explorar un nuevo mecanismo molecular subyacente del extracto del polvo de Huangkui (HKEP) en el alivio de la nefropatía diabética (ND). Los podocitos murinos inmortalizados se dividieron en (i) grupos de glucosa normal (NG, 5,6 mM), (ii) NG + HKEP (0,45 g/L), (iii) HG y (iv) HG + HKEP (0,45 g/L). Se utilizaron el ensayo MTT y la citometría de flujo para detectar la proliferación de podocitos, la apoptosis y el ciclo celular. La viabilidad celular se inhibió y la apoptosis aumentó en el grupo (iii) HG en comparación con el grupo (i) NG (p<0,05). La expresión de ARNm y proteínas de nefrina y podocina disminuyó significativamente en el grupo (iii) HG en comparación con el grupo (i) NG (p<0,05). En comparación con el grupo (iii) HG, el grupo (iv) HG + HKEP tuvo una mayor viabilidad celular, una tasa de apoptosis más baja y una expresión de ARNm y proteínas más altas de nefrina y podocina (p<0,05). HKEP puede atenuar el daño de los podocitos inducido por HG, que puede ser uno de los mecanismos de HKEP para atenuar la DN.

Plant Extracts/administration & dosage , Diabetic Nephropathies/drug therapy , Podocytes/drug effects , Powders , Plant Extracts/genetics , Cell Cycle , Blotting, Western , Apoptosis/drug effects , Cell Culture Techniques , Reverse Transcriptase Polymerase Chain Reaction , Glucose
Bol. latinoam. Caribe plantas med. aromát ; 20(3): 215-225, may. 2021. ilus, tab
Article in English | LILACS | ID: biblio-1342813


This review describes the geographical distribution, botanical data, popular use, chemical composition, pharmacological activities and genetic aspects related to Eugenia luschnathiana, a native Brazilian plant popularly known as "bay pitomba". E. luschnathiana leaves are characterized morphologically by the presence of a petiole, an attenuated base, acuminated apex, elliptical shape, and parallel venation. The major chemical compounds found in E. luschnathiana are sesquiterpenes. Literature reports showed that E. luschnathiana extracts have antioxidant properties and antimicrobial activity against Gram-negative and Gram-positive bacteria. The extractsfrom the leaf, fruit and stem, and a concentrated residual solution of its essential oil, displayed negligible toxicity. Lastly, a cytogenetic analysis indicated that some markers can be used for the study of genetic diversity, population structure, and genetic improvements. The information available on E. luschnathiana supports the hypothesis that this plant may be a source of compounds with promising pharmacological activity.

Esta revisión describe la distribución geográfica, datos botánicos, uso popular, composición química, actividad farmacológica y el análisis genético de Eugenia luschnathiana, una planta originaria del Brasil conocida popularmente como "pitomba da baía". Las hojas de E. luschnathiana se caracterizan por la presencia de pecíolo, base atenuada, ápice acuminado, forma elíptica y venación paralela. Su composición química presenta mayormente sesquiterpenos. Los informes en la literatura muestran que los extractos de E. luschnathiana presentan propiedades antioxidantes y actividad antimicrobiana contra las bacterias Gram-negativas y Gram-positivas. Los extractos de la hoja, fruto y tallo, y una solución residual concentrada del aceite esencial, presentaron baja toxicidad. Por último, un análisis citogenético indicó que algunos marcadores pueden utilizarse para estudios de diversidad genética, estructura poblacional y mejoramiento genético. Las informaciones disponibles acerca de E. luschnathiana proponen la hipótesis de que esta planta puede ser una fuente de compuestos con actividad farmacológica prometedora.

Oils, Volatile/pharmacology , Plant Extracts/pharmacology , Eugenia/chemistry , Anti-Infective Agents/pharmacology , Terpenes/analysis , Bacteria/drug effects , Oils, Volatile/chemistry , Plant Extracts/genetics , Plant Extracts/chemistry , Plant Leaves/chemistry , Eugenia/genetics , Medicine, Traditional , Anti-Infective Agents/chemistry
Genomics, Proteomics & Bioinformatics ; (4): 230-240, 2020.
Article in English | WPRIM | ID: wpr-880486


Scutellaria baicalensis (S. baicalensis) and Scutellaria barbata (S. barbata) are common medicinal plants of the Lamiaceae family. Both produce specific flavonoid compounds, including baicalein, scutellarein, norwogonin, and wogonin, as well as their glycosides, which exhibit antioxidant and antitumor activities. Here, we report chromosome-level genome assemblies of S. baicalensis and S. barbata with quantitative chromosomal variation (2n = 18 and 2n = 26, respectively). The divergence of S. baicalensis and S. barbata occurred far earlier than previously reported, and a whole-genome duplication (WGD) event was identified. The insertion of long terminal repeat elements after speciation might be responsible for the observed chromosomal expansion and rearrangement. Comparative genome analysis of the congeneric species revealed the species-specific evolution of chrysin and apigenin biosynthetic genes, such as the S. baicalensis-specific tandem duplication of genes encoding phenylalanine ammonia lyase and chalcone synthase, and the S. barbata-specific duplication of genes encoding 4-CoA ligase. In addition, the paralogous duplication, colinearity, and expression diversity of CYP82D subfamily members revealed the functional divergence of genes encoding flavone hydroxylase between S. baicalensis and S. barbata. Analyzing these Scutellaria genomes reveals the common and species-specific evolution of flavone biosynthetic genes. Thus, these findings would facilitate the development of molecular breeding and studies of biosynthesis and regulation of bioactive compounds.

Evolution, Molecular , Flavonoids/biosynthesis , Genome, Plant , Plant Extracts/genetics , Scutellaria/metabolism , Whole Genome Sequencing
Indian J Biochem Biophys ; 2012 Feb; 49(1): 36-41
Article in English | IMSEAR | ID: sea-140216


A gene OsZnI encoding Cys3/His1-type zinc finger protein was isolated from the water stress-induced cDNA library of rice (Oryza sativa) cv. N-22, an early maturing, deep-rooted, drought-tolerant genotype adapted to upland conditions. The in-silico analysis revealed an insert of 800 bp with an ORF of 663 nucleotides, encoding 221 amino acids. OsZnI had three distinct features — nuclear localization signal (NLS) present in Arg152-Arg168, Zn finger domain between 185-193 amino acids and 12 amino acids conserved domain in 71-82 amino acids homologous to LEA motif, and belonged to C-type family of Zn finger protein. OsZnI showed induced expression under water deficit stress.

Amino Acid Sequence/genetics , Base Sequence/genetics , Cloning, Molecular/methods , Conserved Sequence/genetics , Dehydration/genetics , Droughts , Genes, Plant/genetics , Molecular Sequence Data , Oryza/genetics , Plant Extracts/genetics , Plant Extracts/metabolism , Plant Leaves/genetics , Plant Leaves/metabolism , Plant Proteins/genetics , Sequence Homology, Amino Acid , Transcription Factors/genetics , Zinc Fingers/genetics
Southeast Asian J Trop Med Public Health ; 2005 ; 36 Suppl 4(): 238-41
Article in English | IMSEAR | ID: sea-35873


Thunbergia laurifolia Linn has been reputed to have antitoxic effects for all toxic substances. In this present study, we evaluated its effect against the mutagenicity induced by aqueous extracts from Pueraria mirifica Airy Shaw & Suvatabundhu in male rats. The formation of micronuclei in polychromatic erythrocytes was induced by oral administration of an aqueous extract of P. mirifica at the doses of 400, 600, and 800 mg/kg to the rats for 30 days. The results were that the extracts of P. mirifica at doses of 600 and 800 mg/kg acted as a mutagenic agent by inducing higher frequencies of micronuclei as compared to the controls. For the antimutagenic test, P. mirifica extract at a dose of 600 mg/kg (minimal effective dose) was mixed with fresh and dried extracts of T. laurifolia in proportions of 7:3 and 1:1, respectively. The results of 4-week-treatment indicated that aqueous extracts of T. laurifolia, prepared by both fresh and dry methods, could significantly inhibit the induction of micronuclei as induced by P. mirifica. It could be concluded from the results that, under certain circumstances, T. laurifolia exhibits a significant antimutagenic activity. The use of P. mirifica and T. laurifolia as fusion herbal medicines is suggested.

Acanthaceae , Animals , Antitoxins/pharmacology , Erythrocytes/drug effects , Male , Micronuclei, Chromosome-Defective/chemically induced , Mutagenicity Tests , Mutagens/toxicity , Plant Extracts/genetics , Pueraria/toxicity , Rats
Rev. cuba. plantas med ; 5(3): 91-6, sept.-dic. 2000. tab
Article in Spanish | LILACS | ID: lil-295510


Con el objetivo de conocer la posible actividad mutagénica del extracto fluido de Cassia grandis L. y el gel de Aloe vera L., que puedan representar algún efecto adverso para su uso en la fitoterapia, se llevó a cabo un estudio toxicogenético empleando 2 sistemas de ensayo a corto plazo uno in vitro y otro in vivo; el modelo Aspergillus nidulans D-30 que detecta daño primario al ADN y el ensayo de inducción de micronúcleos en médula ósea de ratón el cual determina daño clastogénico y aneugénico. En el ensayo in vitro con el hongo Aspergillus nidulans D-30 (segregación mitótica) se evaluaron concentraciones del extracto fluido de Cassia grandis L., desde 0,067 a 1,675 mg de sólidos totales/mL y para el gel de Aloe vera L., concentraciones de 0,09 a 1,00 mg de sólidos totales/mL. En la prueba in vivo de inducción de micronúcleos se ensayaron para la Cassia grandis L. y para el gel de Aloe vera L., dosis de 500, 1 000 y 2 000 mg/kg de peso corporal (pc). En ambas baterías de ensayos genotóxicos ninguno de los 2 fitofármacos mostraron ni daño celular ni actividad genotóxica

Aloe/genetics , Aloe/toxicity , Aspergillus nidulans , Herbal Medicine , Mutagenicity Tests , Plant Extracts/genetics , Plant Extracts/toxicity , Plants, Medicinal , Micronucleus Tests , Mice
Rev. cuba. plantas med ; 1(2): 18-23, mayo-ago. 1996. tab
Article in Spanish | LILACS | ID: lil-186772


Se realizo la evaluacion mutagenica de un extracto acuoso liofilizado de hojas de Aloe vera L., para lo cual se emplearon tres ensayos a corto plazo: induccion de mutaciones puntuales (supresores) en el locus meth G1 de Aspergillus nidulans, segregacion mitotica en un diploide heterocigotico de Aspergillus nidulans y el test de micronucleos en medula osea de raton. Para los ensayos in itro se eavaluaron concentraciones entre 0,05 y 5 mg de extracto de aloe/mL en medio de cultivo (mutaciones puntuales) y de 0,04 a 1 mg/mL (segregacion mitotica); se empleo el metodo de incorporacion en placa. No se detectaron aumentos significativos para la frecuencia de mutantes supresores en el primer ensayo, ni de sectores segregantes homocigoticos en el segundo, que son indicadores de genotoxicidad para estas pruebas. En el ensayo in vivo se emplearon ratones de la linea isogenica suizo, a los que se hicieron dos administraciones del extracto por via intragastrica, en dosis de 0,5 1,0 y 2,0 g/kg/dia, con sacrificio 24 horas despues de la ultima aplicacion. En ningun caso se detecto efecto citotoxico sobre la proliferacion celular en la medula osea, ni aumentos significativos en la frecuencia de eritrocitos policromaticos micronucleados 2(mPCE), indicador de mutagenicidad para este ensayo

Aloe/genetics , Plant Extracts/genetics , Micronucleus Tests/methods