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1.
Rev. Asoc. Odontol. Argent ; 108(1): 19-24, ene.-abr. 2020. ilus
Article in Spanish | LILACS (Americas) | ID: biblio-1096713

ABSTRACT

Objetivo: Presentar la terapia regenerativa como una al- ternativa para la resolución de un caso de traumatismo denta- rio en un diente permanente incompletamente desarrollado. Caso clínico: Se realizó el tratamiento de un incisivo central superior con mortificación pulpar y periodontitis api- cal aguda subsecuente a trauma dental en un paciente de 8 años de edad. Se aplicó el protocolo de regeneración pulpar recomendado por la Asociación Americana de Endodoncia. Se estimuló la formación de un coágulo en el interior del con- ducto a partir de los tejidos periapicales, previa desinfección con la pasta triple antibiótica, y finalmente se colocó mineral trióxido agregado coronal a este. Se obtuvo así una matriz es- teril que permitió el crecimiento de nuevo tejido y se realiza- ron controles periódicos durante 4 años. Se constató silencio clínico. Radiográficamente, se observó la formación de tejido sobre las paredes del conducto y el cierre apical. Conclusión: La terapia regenerativa como alternativa de tratamiento, en este caso, permitió la disminución de la luz del conducto por el depósito de tejidos calcificados y el cierre del foramen apical, mejorando el pronóstico de la pieza dentaria (AU)


Aim: To present pulp regeneration therapy as an alter- native to resolve dental trauma in immature permanent teeth. Clinical case: We report a clinical case of an immature central superior incisor with pulp mortification and acute api- cal periodontitis subsequent to dental trauma, in an 8 year old patient. The pulp Regeneration protocol recommended by the American Endodontics Association was applied. We stimulated a clot formation inside the duct from periapical tissues and after disinfection with a mixture of three antibi- otics mineral trioxide aggregated was finally place coronal to the clot. Thus a sterile matrix was obtained that allowed new tissue's growth. Periodic check-up visits were carried out over a 4 years period. Clinical silence was observed. Tissue formation on duct walls and apical closure were radiograph- ically detected (AU) Conclusion: Regenerative therapy is an alternative for the treatment of immature permanent teeth, in ths clinical case it allowed the reduction of the width of the duct by the opposition of hard tissues and the closure of the apical fora- men improving the forecast of these teeth.


Subject(s)
Regeneration , Dentition, Permanent , Incisor/injuries , Periapical Periodontitis , Argentina , Root Canal Filling Materials/therapeutic use , Wound Healing/physiology , Tooth Injuries/complications , Dental Pulp Necrosis , Tooth Apex/growth & development , Dental Service, Hospital
2.
Int. j. morphol ; 37(4): 1234-1244, Dec. 2019. graf
Article in Spanish | LILACS (Americas) | ID: biblio-1040118

ABSTRACT

La cicatrización de la piel es un proceso complejo y organizado que involucra tres fases: inflamatoria, proliferativa y de remodelación. Es indispensable el análisis de este proceso biomolecularmente para investigar y proponer nuevas estrategias terapéuticas que mejoren la cicatrización o promuevan la regeneración. El objetivo de este proyecto fue analizar histológica y biomolecularmente mediante microespectroscopía infrarroja por transformada de Fourier (MFTIR) y su función de mapeo bioquímico, muestras de lesiones excisionales de piel, comparando los cambios morfológicos y espectroscópicos entre piel sana y piel cicatrizada. Se estandarizó un modelo de lesión excisional de piel en ratones hembra de la cepa NIH de 8 semanas de edad (n=16), provocando una herida excisional de 1 cm2. Se analizó piel sana (día 0) y cicatrizada (día 15 post-lesión) morfométrica, histológica y biomolecularmente mediante análisis fotográfico, técnica histológica y MFTIR con su función de mapeo. El análisis morfométrico demostró una reducción del área de la herida en un 87,6 % al día 15 post-lesión. Histológicamente, en la piel cicatrizada se evidenció un adelgazamiento de la epidermis y menor celularidad en la dermis, observándose la formación de tejido de granulación y fibras de colágena desorganizadas. Espectroscópicamente, se apreciaron cambios entre los dos grupos de estudio, principalmente en las bandas de lípidos y en la región de proteínas. El cálculo de las áreas bajo la curva y el mapeo bioquímico mostraron menor concentración de queratina y colágena en la piel cicatrizada, así como desorganización de las fibras de colágena. Se demostró la capacidad de la MFTIR para caracterizar de forma precisa los cambios biomoleculares en la cicatrización, entre ellos la cantidad de queratina, colágena, y el depósito y ordenamiento de las fibras de colágena asociadas a su maduración.


The skin cicatrization is a complex and organized process that involves three phases: inflammatory, proliferative, and remodeling. It is essential to analyze this process biomolecularly, in order to investigate and propose new therapeutic strategies that improve the healing or promote regeneration. The objective of this project was to analyze histological and biomolecularly through Fourier Transform infrared microspectroscopy (FTIRM) and its biochemical mapping function, samples of an excisional skin wound, comparing the morphological and spectroscopic changes between healthy skin and scarred skin. An excisional skin wound healing model was standardized using female, NIH strain 8-week-old mice (n = 16), provoking an excisional wound of 1 cm2. Healthy skin (day 0) and scarring skin (day 15 post-injury) were morphometrical, histological, and biomolecularly analyzed by digital picture analysis, histological technique, and FTIRM with its mapping function. The morphometric analysis showed a reduction of the wound area of 87.6 % at day 15 after wound. Histologically, in the scarred skin a thinning of the epidermis was evidenced, besides reduced cellularity in the dermis, granulation tissue formation, and disorganized collagen fibers were observed. Spectroscopically, changes between the study groups were appreciated, mainly in the lipid bands and in the protein region. The calculation of the areas under the curve and the biochemical mapping showed a lower concentration of keratin and collagen in the scarred skin, as well as collagen fibers disorganization. The ability of the FTIRM to accurately characterize biomolecular changes in cicatrization process was demonstrated, such as the amount of keratin, collagen, and the deposition and ordering of the collagen fibers associated with their maturation.


Subject(s)
Animals , Female , Mice , Skin/injuries , Wound Healing/physiology , Spectroscopy, Fourier Transform Infrared , Skin/pathology , Skin Physiological Phenomena , Disease Models, Animal
3.
Fisioter. Pesqui. (Online) ; 26(2): 164-169, abr.-jun. 2019. graf
Article in English | LILACS (Americas) | ID: biblio-1012148

ABSTRACT

ABSTRACT Nicotine delays the healing process and increases the levels of myeloperoxidase (MPO), an enzyme that plays a key role in the production of reactive oxygen species during the inflammatory process. Laser Photobiomodulation (PBM) is one of the most used electrophysical agents in the treatment of the calcaneal tendon, however, its effects on MPO activity need to be further elucidated. This study aimed to evaluate the effects of laser PBM on MPO activity after inflicting an injury to the calcaneal tendon of rats exposed to cigarette smoke. Thirty-four male Wistar rats with 90 days of age were used. After 14 days of exposure to cigarette smoke, the animals were divided into three experimental groups: control group (CG, n=12), not submitted to injury or treatment; sham group (ShG, n=10), submitted to partial calcaneal tendon injury and laser PBM simulation; and laser PBM group (PBMG, n=12), submitted to partial calcaneal tendon lesion and treated with laser PBM within the first minute after injury. PBM decreased MPO activity levels in PBMG compared to ShG (CG: 1.38±0.69pg/ml; ShG: 3.78±1.09pg/ml; PBMG: 2.58±0.93pg/ml; p<0.005). In conclusion, applying laser PBM immediately after inflicting damage to the calcaneal tendon attenuates acute inflammatory activity in rats exposed to cigarette smoke.


RESUMO A nicotina retarda o processo de cicatrização e eleva os níveis da enzima mieloperoxidase (MPO), a qual possui um papel fundamental na produção de espécies reativas de oxigênio durante o processo inflamatório. A fotobiomodulação laser (FBM) é um dos agentes eletrofísicos mais utilizados no tratamento do tendão calcâneo, no entanto, os seus efeitos sobre a atividade da MPO carecem de maior elucidação. Este estudo objetivou avaliar os efeitos da FBM sobre a atividade da MPO, após lesão do tendão calcâneo em ratos expostos à fumaça de cigarro. Foram utilizados 34 ratos Wistar, machos, com 90 dias de vida. Após 14 dias de exposição à fumaça de cigarro, os animais foram divididos em três grupos experimentais: grupo controle (GC, n=12), não submetido à lesão ou tratamento; grupo sham (GSh, n=10), submetido à lesão parcial do tendão calcâneo e a simulação da FBM laser; grupo FBM laser (GFBM, n=12), submetido à lesão parcial do tendão calcâneo e tratados com FBM laser, no primeiro minuto após a lesão. A FBM diminuiu os níveis de atividade da MPO no GFBM em comparação ao GSh (GC: 1,38±0,69 pg/ml; GSh: 3,78±1,09pg/ml; GFBM: 2,58±0,93pg/ml; p<0,005). Conclui-se que a FBM laser aplicada imediatamente após lesão do tendão calcâneo, atenua a atividade inflamatória aguda em ratos expostos à fumaça de cigarro.


RESUMEN La nicotina retarda el proceso de cicatrización y eleva los niveles de la enzima mieloperoxidasa (MPO), que tiene un papel fundamental en la producción de especies reactivas de oxígeno durante el proceso inflamatorio. La fotobiomodulación con láser (FBM) es uno de los agentes electrofísicos más utilizados en el tratamiento del tendón calcáneo, sin embargo sus efectos sobre la actividad de la MPO carecen de mayor elucidación. Este estudio objetivó evaluar los efectos de la FBM sobre la actividad de la MPO después de lesión del tendón calcáneo en ratones expuestos al humo de cigarrillo. Se utilizaron 34 ratones Wistar, machos, con 90 días de vida. Después de 14 días de exposición al humo de cigarrillo, los animales fueron divididos en tres grupos experimentales: grupo de control (GC, n=12), no sometido a la lesión o tratamiento; grupo sham (GSh, n=10), sometido a la lesión parcial del tendón calcáneo y a la simulación de la FBM láser; y el grupo FBM láser (GFBM, n=12), sometido a la lesión parcial del tendón calcáneo y tratado con FBM láser, en el primer minuto después de la lesión. La FBM disminuyó los niveles de actividad de MPO en el GFBM en comparación con el GSh (GC: 1,38±0,69 pg/ml; GSh: 3,78±1,09pg/ml; GFBM: 2,58±0,93pg/ml, p<0,005). Se concluye que la FBM láser aplicada inmediatamente después de la lesión del tendón calcáneo atenúa la actividad inflamatoria aguda en ratones expuestos al humo de cigarrillo.


Subject(s)
Animals , Achilles Tendon/physiopathology , Tobacco Smoke Pollution/adverse effects , Low-Level Light Therapy , Tendinopathy/therapy , Wound Healing/physiology , Rats, Wistar , Models, Animal , Inflammation/physiopathology , Nicotine/adverse effects
4.
Acta cir. bras ; 34(7): e201900701, 2019. tab, graf
Article in English | LILACS (Americas) | ID: biblio-1038120

ABSTRACT

Abstract Purpose: To compare the use of new cyanoacrylate surgical adhesive associated with macroporous tapes in cutaneous synthesis. Methods: Male Wistar rats with a longitudinal incision of 4cm were used on the back, divided into four groups: GI used octyl-cyanoacrylate (Dermabond®), GII used N-2-butylcyanoacrylate, GIII used octyl-cyanoacrylate and macroporous tape and GIV used N-2-butyl cyanoacrylate and macroporous tape. On the fourteenth day, the rats were submitted to euthanasia, were divided in two parts, and a layer of skin subcutaneous tissue through an area of operative healing was removed. One part was submitted to the study of rupture strength with the use of tensiometer, and in the other part histological examination was performed. Results: No force test was similar between groups I and II, being different from groups III and IV (P <0.001), which were identical to each other (P> 0.05). The units were compared among the studied groups, and they were different with the use of macroporous tapes (P> 0.05). Conclusions: The purpose of macroporous tapes is associated with CA adhesives in cutaneous tissues that provide more resistant scars. The use of a combination of macroporous tapes leads to complete re-epithelialization, without provoking foreign body reaction, has hemostatic properties and does not cause an absorptive reaction.


Subject(s)
Animals , Male , Rats , Skin/drug effects , Surgical Wound Dehiscence/prevention & control , Tissue Adhesives/pharmacology , Wound Healing/physiology , Enbucrilate/pharmacology , Suture Techniques , Rats, Wistar
5.
Acta cir. bras ; 34(12): e201901203, 2019. graf
Article in English | LILACS (Americas) | ID: biblio-1054686

ABSTRACT

Abstract Purpose Composite flaps used in reconstructive surgery may intra- and postoperatively suffer from hypoperfusion and/or ischemia-reperfusion influencing wound healing. We aimed to follow-up the effect of ischemia on adipocutaneous flaps' wound healing and microcirculation. Methods In anesthetized rats groin flaps were formed bilaterally. In Control group the flaps were repositioned and sutured back. In Ischemia-Reperfusion (I/R) group before repositioning and suturing the flap pedicles were clamped for 60 minutes. Laser Doppler (LD) fluxmetry and temperature probes were applied on the cranial, central and caudal flap regions before/after preparation and ischemia, re-suturing, and on the 1st-3rd-5th-7th-14th postoperative days, before the final examinations and biopsies for histology. Results Flaps' skin temperature quickly recovered after repositioning. LD values were lower in the I/R group, reaching a significant level by the 3rd postoperative day, and remained lowered till the 14th day. The magnitude of alterations differed in the flap regions. Histologically normal wound healing process was seen, except for some I/R flaps, where hypertrophized mammary glands were found. Conclusions Short-term ischemia could influence flap microcirculation and wound healing, and may result in hypertrophized mammary glands. Laser Doppler could be used to evaluate intra- and postoperative microcirculatory changes and may have significance in predicting complications.


Subject(s)
Animals , Male , Rats , Skin/blood supply , Wound Healing/physiology , Reperfusion Injury/complications , Dermatologic Surgical Procedures/adverse effects , Myocutaneous Flap/blood supply , Microcirculation/physiology , Postoperative Period , Reference Values , Skin/pathology , Time Factors , Biopsy , Body Temperature , Reperfusion Injury/pathology , Reproducibility of Results , Treatment Outcome , Laser-Doppler Flowmetry , Disease Models, Animal , Myocutaneous Flap/pathology
6.
Acta cir. bras ; 34(6): e201900608, 2019. tab, graf
Article in English | LILACS (Americas) | ID: biblio-1019267

ABSTRACT

Abstract Purpose To analyze the resistance to medial traction of abdominal wall muscles, before and after performing relaxing incisions. Methods Seventeen live pigs were used. After a median laparotomy, the handles were made in the rectus abdominis muscles (RAM) to fit the dynamometer. Step 1 (control phase): tensile strength measured without performing relaxant incisions. Step 2: A curvilinear relaxant incision was made on the anterior blade of the right RAM sheath and then the tensile strength was measured by the edge of the wound. The same procedure was adopted after incision of the left posterior blade. Step 3: Relaxing incisions were made in the right posterior and left anterior blade, so that both sides were left with a relaxing incision on both blades. Measurements of resistance were performed. Results There was no statistically significant difference between the sides. On the right and left side, all treatments reduced the tensile strength when compared to each other and to the control. There was a reduction of 12% and 9.8% after incision of the anterior and posterior blade, respectively. Conclusion Relaxing incisions reduced tensile strength in the ventral abdominal wall.


Subject(s)
Animals , Tensile Strength/physiology , Abdominal Muscles/surgery , Abdominal Wall/surgery , Laparotomy/methods , Swine , Wound Healing/physiology , Suture Techniques , Abdominal Muscles/physiology
7.
Acta cir. bras ; 33(9): 762-774, Sept. 2018. tab, graf
Article in English | LILACS (Americas) | ID: biblio-973507

ABSTRACT

Abstract Purpose: To compare wound healing performed with cold blade (CSB) and ultrasonic harmonic scalpel (UHS) in the abdominal aponeurosis of rats. Methods: Eighty Wistar rats divided into two groups and underwent midline incision in the linea alba with cold blade and harmonic ultrasonic scalpel. Analysis were performed in subgroups of 10 animals after 3, 7, 14 and 21 days. Macroscopically was observed the presence of hematoma, infection, wound dehiscence, fistula and adherences. Microscopically were used collagen and immunohistochemical staining methods. Results: Macroscopic, complications showed no statistical difference. Immunohistochemical analysis for MMP-9 was more intense in UHS group (p<0.05). TGF β presented its lower expression in UHS group at 14 and 21 days, with no statistical difference at 3 and 7 days (p<0.05). α-AML expression appeared higher in UHS group after 14 days and remained similar in others (p<0.05). Collagen deposition had no change in type I, and increased in type III in UHS; at 7th day the deposition was higher in CSB group; at 14th was similar in both groups (p<0.001). Conclusion: UHS compared to the CSB has higher lesion area at the time of the incision; as well as it led to the delay of regeneration and scar maturation process.


Subject(s)
Animals , Male , Rats , Wound Healing/physiology , Collagen/physiology , Abdominal Wall/surgery , Surgical Wound/pathology , Surgical Instruments , Immunohistochemistry , Rats, Wistar , Models, Animal , Abdominal Wall/pathology , Tissue Array Analysis , Ultrasonic Surgical Procedures , Surgical Wound/physiopathology
8.
Rev. chil. cir ; 70(4): 350-353, ago. 2018. ilus
Article in Spanish | LILACS (Americas) | ID: biblio-959394

ABSTRACT

Resumen Introducción: Una de las formas de presentación de la enfermedad pilonidal sacrococcígea (EPSC) es el absceso, para el que existen distintas alternativas de tratamiento. Objetivo: Presentar nuestra experiencia con la técnica de Bascom para el tratamiento de la EPSC abscedada. Material y Método: Serie prospectiva, consecutiva y no aleatoria. Incluye todos los pacientes mayores de 15 años que presentan un absceso o supuración masiva al momento de la cirugía. Resultados: La serie corresponde a 10 pacientes, 7 de género masculino. No se encuentran los factores de riesgo reconocidos por la literatura como riesgo de EPSC. Ocho pacientes mejoran completamente en un período máximo de 18 días y 2 presentan supuración persistente, por lo que se considera fracaso del tratamiento. Estos son sometidos a una segunda cirugía con otra técnica, con curación de la enfermedad. Conclusión: La técnica de Bascom es sencilla, segura y ofrece una curación de un 80% en un período corto de cicatrización.


Introduction: One of the form of presentation of the sacral coccygeal pilonidal disease is the abscess, for this cases there are various treatment alternatives. Objective: We present our experience with Bascom technique for the treatment of pilonidal abscess. Material and Method: Prospective, consecutive, non-randomized series. It includes all patients older than 15 years who have an abscess or mass discharge at the time of surgery. Results: The series consists of 10 patients, 7 males. They are not risk factors recognized in the literature as risk of pilonidal disease. Eight patients completely better within a maximum period of 18 days and two with persistent discharge by what is considered treatment failure. They were subjected to a second surgery treatment with another technique with good results. Conclusion: Bascom's technique is simple, safe and offers a 80% cure in a short period of healing.


Subject(s)
Humans , Male , Female , Pilonidal Sinus/surgery , Surgical Procedures, Operative/methods , Wound Healing/physiology , Abscess/surgery , Reoperation , Sacrococcygeal Region , Buttocks/surgery , Drainage/methods , Prospective Studies , Follow-Up Studies , Suture Techniques , Treatment Outcome
9.
Rev. Asoc. Méd. Argent ; 131(2): 7-26, jun. 2018. tab, ilus
Article in Spanish | LILACS (Americas) | ID: biblio-973098

ABSTRACT

Se revisan los nuevos conocimientos sobre la matriz extracelular (MEC), que han permitido descubrir su importante rol en la cicatrización de las heridas cutáneas. Se describen sus características morfofisiológicas y cómo interviene en la curación de las heridas cutáneas. Se presentan cuatro casos clínicos en los que se aplicó este enfoque terapéutico: los sustitutos de piel y la “cura húmeda”.


We review the new knowledge about the extracellular matrix (ECM) that has allowed us to discover its important role in the healing of cutaneous wounds. The morphophysiological characteristics of ECM and its role in the healing of cutaneous wounds are described. Four clinical cases are presented where this therapeutic approach was applied: the skin substitutes and the “moist wound healing”.


Subject(s)
Male , Female , Humans , Adolescent , Middle Aged , Aged , Extracellular Matrix/metabolism , Extracellular Matrix/genetics , Skin/injuries , Wound Healing/physiology , Cell Differentiation , Electromagnetic Radiation , Regenerative Medicine
10.
Acta cir. bras ; 33(5): 454-461, May 2018. graf
Article in English | LILACS (Americas) | ID: biblio-949348

ABSTRACT

Abstract Purpose: To evaluate abdominal ventral wound healing by using a specific biomaterial, a handmade polyamide surgical mesh. Methods: A surgical incisional defect was made in ten rabbits to simulate a hernia in the ventral abdominal musculature. A polyamide surgical mesh was used in hernioplasty. They were monitored for surgical wound healing, and macroscopically and histologically evaluated at the end of the experiment. The polyamide surgical mesh did not cause foreign body reaction, pain, edema, or infection in the surgical site. The manure production was not affected by intestinal tissue adherences to the mesh, consistent with the ultrasonography result where adherences were not observed and organized scarring tissue formed in the incisional defect. The polyamide mesh was fixed over the abdominal wall, and its external and internal sides were surrounded by a vascularized connective tissue. Results: None of the experimental animals developed adherences from internal organs to the polyamide mesh, except two rabbits where the omentum formed adherence to the internal scarring tissue without present herniation or compromise of the rabbit's health. Conclusion: Polyamide surgical mesh for hernioplasty presents, in rabbits, excellent biocompatibility, with minimal body adverse reactions and low cost.


Subject(s)
Animals , Male , Rats , Prostheses and Implants , Surgical Mesh , Wound Healing/physiology , Abdominal Wall/surgery , Herniorrhaphy/adverse effects , Hernia, Ventral/surgery , Biocompatible Materials , Tissue Adhesions/physiopathology , Abdominal Muscles/transplantation
11.
Rev. cuba. enferm ; 34(1): e1440, ene.-mar. 2018. tab, graf
Article in Portuguese | LILACS (Americas), CUMED | ID: biblio-1099027

ABSTRACT

RESUMO Introdução: As lesões cutâneas acometem a população brasileira de forma geral, independente de idade, sexo ou etnia, responsáveis por um alto índice de casos relacionados às alterações na integridade da pele, constituindo-se em um importante problema de saúde pública. Objetivo: Caracterizar os estudos publicados em bases eletrônicas produzidos pela enfermagem brasileira sobre feridas. Métodos: Revisão integrativa, realizada em março de 2016 nas bases de dados eletrônicas LILACS, SciELO, BDENF, PUBMED e Web of Science. Foram critérios de inclusão: artigos completos, disponíveis nas bases de dados eletrônicas elencadas, que tratem dos cuidados de enfermagem às lesões cutâneas. Conclusão: Identificou-se que os estudos da enfermagem do Brasil focam especialmente o tratamento tópico da lesão em âmbito hospitalar, quando também desconsidera outros pontos primordiais na assistência ao portador de lesão cutânea, como os cuidados prestados na atenção básica, os aspectos emocionais, o aporte nutricional, os custos do tratamento, a capacitação profissional e a construção de protocolos que norteiam a sistematização da produção do cuidado(AU)


RESUMEN Introducción: las lesiones cutáneas que afectan a la población brasileña en general, independientemente de su edad, género o etnia, son responsables de un gran número de casos relacionados con los cambios en la integridad de la piel, convirtiéndose así en un importante problema de salud pública. Objetivo: caracterizar los estudios publicados sobre heridas en bases de datos electrónicas para las enfermeras brasileñas. Métodos: una revisión integradora, celebrada en marzo de 2016, las bases de datos electrónicas LILACS, SciELO, BDENF, PubMed y Web of Science. Los criterios de inclusión fueron: artículos completos disponibles en bases de datos electrónicas de la lista, que aborden la atención de enfermería para lesiones de la piel. Conclusión: se encontró que los estudios de enfermería de Brasil se centran en especial el tratamiento tópico de las lesiones en el ambiente hospitalario, donde también ignora otras cosas esenciales en el cuidado de pacientes con lesiones de la piel, tales como el cuidado en la atención primaria, los aspectos emocionales, el apoyo nutricional, los costos de tratamiento, la formación profesional y la construcción de protocolos que guían la sistematización de la producción del cuidado(AU)


ABSTRACT Introduction: Cutaneous lesions affecting the Brazilian population in general, regardless of age, gender or ethnicity, responsible for a high number of cases related to changes in the integrity of the skin, thus becoming a major public health problem. Objective: To characterize the studies published in electronic databases for Brazilian nurses on wounds. Methods: An integrative review, held in March 2016 in electronic databases LILACS, SciELO, BDENF, PubMed and Web of Science. Inclusion criteria were: full articles available on listed electronic databases that address nursing care for skin lesions. Conclusion: It was found that Brazil's nursing studies focus especially the topical treatment of lesions in the hospital environment, where also ignores other essential things in care for patients with skin lesions, such as care in primary care, the emotional aspects, the nutritional support, treatment costs, professional training and the construction of protocols that guide the systematization of care production(AU)


Subject(s)
Humans , Primary Nursing/methods , Wound Healing/physiology , Degloving Injuries/etiology , Nursing Care/methods , Full-Text Databases
12.
Acta cir. bras ; 33(2): 144-155, Feb. 2018. graf
Article in English | LILACS (Americas) | ID: biblio-886256

ABSTRACT

Abstract Purpose: To evaluate the efficacy of the application of the human amniotic membrane (HAM) on the inflammatory process, fibroblast proliferation, formation of collagenand reduction of skin wound areas in rats. Methods: Thirty six rats were submitted to a surgical injury induction and divided into two groups (n = 18): group C (control) and T (treated with the HAM). The macroscopic evolution in the wound area and the histological characteristics of the skin samples were evaluated. Results: The regression of the wound area was greater in group T. The histological analysis revealed a significant reduction (p < 0.05) in the inflammatory infiltrate in group T at all experimental periods compared with that in the control group. Furthermore, the group T presented a significant increase in the proliferation of fibroblasts at 14 and 21 days compared with group C (p < 0.05). Regarding the deposition of mature collagen fibers, there was an increase in the replacement of type III collagen by type I collagen in group T (p < 0.05). Conclusion: Treatment with the HAM reduced the healing time as well as the inflammatory responses, increased the proliferation of fibroblasts, and induced a higher concentration of mature collagen fibers.


Subject(s)
Humans , Animals , Male , Rats , Skin/injuries , Wound Healing/physiology , Biological Dressings , Collagen/pharmacology , Amnion/transplantation , Skin/pathology , Wound Healing/drug effects , Random Allocation , Rats, Wistar , Collagen Type I/metabolism , Collagen Type I/pharmacology , Collagen Type III/metabolism , Collagen Type III/pharmacology , Disease Models, Animal , Fibroblasts/metabolism , Fibroblasts/pathology , Amnion/chemistry , Inflammation/metabolism
13.
Braz. oral res. (Online) ; 32: e29, 2018. graf
Article in English | LILACS (Americas) | ID: biblio-889503

ABSTRACT

Abstract Concentrated growth factor (CGF) is an autogenuous product that contains highly concentrated number of platelets and can be derived from venous blood by selective centrifugation. It has been speculated that local growth factors in human platelets (insulinlike growth factor, IGF; transforming growth factor, TGF-b; platelet derived growth factor, PDGF) would enhance healing of grafts and also counteract resorption. The osteogensis effect of CGF and acellular dermal matrix (ADM) for alveolar cleft defects was evaluated in this study. Twenty alveolar cleft patients were divided randomly into two groups. One group underwent guided bone regeneration (GBR) using acellular dermal matrix film combined with alveolar bone grafting using iliac crest bone grafts (GBR group), while the other group underwent alveolar bone grafting combined with CGF (CGF group). Cone beam computed tomography (CBCT) images were obtained at 1 week and 6 months following the procedure. Using Mimics 17.0 software, the bone resorption rate and bone density improvement rate were calculated and compared between the two groups. Although not significant between ADM and CGF in bone resorption rate, the bone density improvement in cases with CGF(61.62 ± 4.728%) was much better than in cases with ADM (27.05 ± 5.607%) (p = 0.0002). Thus, CGF could be recommended to patients with alveolar cleft as a better choice.


Subject(s)
Humans , Male , Female , Child , Adolescent , Adult , Young Adult , Acellular Dermis , Alveolar Bone Grafting/methods , Cleft Lip/therapy , Cleft Palate/therapy , Guided Tissue Regeneration/methods , Intercellular Signaling Peptides and Proteins/pharmacology , Osteogenesis/drug effects , Alveolar Bone Loss/diagnostic imaging , Alveolar Bone Loss/physiopathology , Bone Density/physiology , Bone Regeneration/drug effects , Bone Regeneration/physiology , Cleft Lip/diagnostic imaging , Cleft Lip/physiopathology , Cleft Palate/diagnostic imaging , Cleft Palate/physiopathology , Cone-Beam Computed Tomography , Osteogenesis/physiology , Reproducibility of Results , Time Factors , Treatment Outcome , Wound Healing/drug effects , Wound Healing/physiology
14.
Arq. bras. med. vet. zootec. (Online) ; 69(6): 1591-1600, nov.-dez. 2017. ilus, tab, graf
Article in Portuguese | LILACS (Americas), VETINDEX | ID: biblio-910780

ABSTRACT

A cicatrização de feridas é um processo que requer a interação de várias células da derme e epiderme. O objetivo deste trabalho foi avaliar qual o momento da aplicação das células das ADSCs em feridas cutâneas agudas que faria diferença na cicatrização nos primeiros sete dias da lesão. As células-tronco foram isoladas do tecido adiposo de camundongos C57Bl/6 GFP+. Para tanto, foram utilizados 49 camundongos C57Bl/6, divididos em quatro grupos: grupo I (GI/controle; n=14); grupo II (GII; n=14): ADSCs injetadas no d0; grupo III (GIII; n=14): ADSCs injetadas no terceiro dia; e Grupo IV (GIV; n=7): ADSCs injetadas no quinto dia. As avaliações clínicas ocorreram nos dias zero, três, cinco e sete, e as histopatológicas nos dias cinco e sete. Na metodologia proposta, foi observado que o uso de ADSCs aumenta a vascularização, a formação de tecido de granulação, a colagenização e incrementa o número de folículos pilosos em apenas sete dias de avaliação. Além disso, o momento da aplicação das células não repercutiu diferenças significativas nas fases inflamatória e proliferativa do processo de cicatrização das feridas cutâneas.(AU)


Wound healing is a process that requires the interaction of various cells in the dermis and epidermis. The aim of this study was to evaluate the action of ADSCs in the treatment of acute wounds in order to understand if application time of the cells results in a difference in healing the first seven days of injury. The stem cells were isolated from adipose tissue of C57BL / 6 mice GFP +. Thus, we used 49 mice C57BL / 6 divided into four groups: Group I (GI / control, n=14); Group II (GII; n=14): ADSCs injected to the d0; Group III (GIII; n=14): ADSCs injected on the 3rd day, and Group IV (GIV; n=7): ADSCs injected day 5(d5). Clinical evaluations were performed on days 0, 3, 5 and 7 and the histopathology on days 5 and 7. In the proposed methodology, the use of ADSCs increased vascularization, formation of granulation tissue, collagen deposition and increases the number of hair follicles in just seven days of evaluation. In addition, the time of application of the cells did not affect significant differences in the inflammatory and the proliferative phase of wound healing skin.(AU)


Subject(s)
Animals , Mice , Stem Cells , Wound Healing/physiology , Adipose Tissue , Inflammation/veterinary
15.
Acta cir. bras ; 32(12): 1045-1055, Dec. 2017. graf
Article in English | LILACS (Americas) | ID: biblio-886197

ABSTRACT

Abstract Purpose: To evaluate the effect of transforming growth factor β1 (TGF-β1) on tendon-to-bone reconstruction of rotator cuff tears. Methods: Seventy-two rat supraspinatus tendons were transected and reconstructed in situ. At 8 and 16 weeks, specimens of three groups; that is control, L-dose (low dose), and H-dose (high dose) were harvested and underwent a biomechanical test to evaluate the maximum load and stiffness values. Histology sections of the tendon-to-bone interface were identified by hematoxylin-eosin or Masson trichrome stain. Collagen type III was observed by picric acid sirius red staining under polarized light. The level of insulin-like growth factor 1 (IGF-1) and vascular endothelial growth factor (VEGF) was measured by the enzyme-linked immunosorbent assay (ELISA) method. Results: Collagen type III of the H-dose group had a significant difference in histology structure compared with the L-dose group (P<0.05). The maximum load and stiffness decreased significantly in the control group compared with the values of the L-dose and H-dose groups. The stiffness among the three groups differed significantly at the same postoperative time (P<0.05). Interestingly, progressive reestablishment of collagen type III affected tendon-to-bone healing significantly in the later stages. Conclusion: The H-dose was associated with an increased collagen type III morphology stimulated by TGF-β1.


Subject(s)
Animals , Male , Rats , Tendon Injuries/drug therapy , Wound Healing/physiology , Rotator Cuff/surgery , Vascular Endothelial Growth Factors/metabolism , Transforming Growth Factor beta1/metabolism , Rotator Cuff Injuries/surgery , Tendon Injuries/metabolism , Tensile Strength/physiology , Wound Healing/drug effects , Biomechanical Phenomena , Enzyme-Linked Immunosorbent Assay , Rotator Cuff/metabolism , Rats, Sprague-Dawley , Collagen Type III/metabolism , Disease Models, Animal , Elasticity/physiology , Transforming Growth Factor beta1/pharmacology , Muscle Strength/physiology , Fibroblasts/drug effects , Fibroblasts/physiology , Rotator Cuff Injuries/metabolism
16.
Rev. cient. odontol ; 5(2): 744-751, jul.-dic. 2017. ilus
Article in Spanish | LILACS (Americas) | ID: biblio-999142

ABSTRACT

Las perforaciones corticales, penetraciones intramedulares o decorticaciones se realizan a menudo como parte del procedimiento de regeneración ósea guiada (ROG). Su sustento biológico se basa en que mejorarían la angiogénesis y proporcionarían una vía hacia el hueso esponjoso rico en vasos sanguíneos. También se consideran como un "noxious stimuli" (estímulo nocivo) que iniciaría el fenómeno regional acelerado con una respuesta local exuberante facilitando la cicatrización ósea normal. Además, mejorarían la unión o enlace físico entre el injerto óseo y el lecho receptor asegurando estabilidad y fusión de los bloques óseos. Sin embargo, se nos presentan algunas interrogantes relacionadas a su fundamento, a los términos que las describen, a los tamaños, a su cantidad y disposición, al instrumental adecuado para realizarlas y al confort del paciente. (AU)


Cortical perforations, intramedullary penetrations or decortications are often performed as part of the guided bone regeneration (GBR) procedure. Their biological basis is that they would improve angiogenesis, providing a pathway to the medullar bone rich of blood vessels. In addition, cortical perforations would be consider as a "noxious stimuli" that initiate the accelerated regional phenomenon with an exuberant local response facilitating normal bone healing. Moreover, they would improve the physical bonding between the bone graft and the recipient bed ensuring stability and fusion of the bone blocks. However, there are some questions related to its foundation, terms to describe them, their sizes, quantity and disposition, the appropriate instruments to perform them and the comfort of the patient. (AU)


Subject(s)
Humans , Wound Healing/physiology , Bone Regeneration/physiology , Neovascularization, Pathologic
17.
J. appl. oral sci ; 25(5): 465-476, Sept.-Oct. 2017. graf
Article in English | LILACS (Americas) | ID: biblio-893653

ABSTRACT

Abstract Tissue repair is an essential process that reestablishes tissue integrity and regular function. Nevertheless, different therapeutic factors and clinical conditions may interfere in this process of periapical healing. This review aims to discuss the important therapeutic factors associated with the clinical protocol used during root canal treatment and to highlight the systemic conditions associated with the periapical healing process of endodontically treated teeth. The antibacterial strategies indicated in the conventional treatment of an inflamed and infected pulp and the modulation of the host's immune response may assist in tissue repair, if wound healing has been hindered by infection. Systemic conditions, such as diabetes mellitus and hypertension, can also inhibit wound healing. The success of root canal treatment is affected by the correct choice of clinical protocol. These factors are dependent on the sanitization process (instrumentation, irrigant solution, irrigating strategies, and intracanal dressing), the apical limit of the root canal preparation and obturation, and the quality of the sealer. The challenges affecting the healing process of endodontically treated teeth include control of the inflammation of pulp or infectious processes and simultaneous neutralization of unpredictable provocations to the periapical tissue. Along with these factors, one must understand the local and general clinical conditions (systemic health of the patient) that affect the outcome of root canal treatment prediction.


Subject(s)
Humans , Periapical Tissue/physiopathology , Root Canal Therapy/methods , Wound Healing/physiology , Tooth, Nonvital/physiopathology , Tooth, Nonvital/therapy , Periapical Periodontitis/therapy , Root Canal Filling Materials/therapeutic use , Root Canal Irrigants/therapeutic use , Bone Cements/therapeutic use , Calcium Hydroxide/therapeutic use , Treatment Outcome
18.
An. bras. dermatol ; 92(4): 474-477, July-Aug. 2017. tab, graf
Article in English | LILACS (Americas) | ID: biblio-887001

ABSTRACT

Abstract: Background: Surgical sutures, wound tension, additional skin incisions and other factors may result in recurrence of tumor-like scar. Objective: To investigate the role of wound natural healing therapy in tumor-like hypertrophic scar. Methods: In this study, tumor-like hypertrophic scars of 47 cases were excised completely and the residual wounds were treated with natural healing. The short-term and long-term effects of treatment were evaluated. Results: All cases were successfully cured by natural healing therapy. The healing time of the maximum wound (80mm × 20mm) and the minimal wound (5mm× 5mm) was 25 days and 7 days respectively. The size of new skin scars ranged from 3mm to 11 mm. Clinical followed-up was performed in 34 cases for 36 months. Among them, no recurrence happened in 31 cases and new scar size ranged from 2mm to 8mm, while local recurrence happened in 3 cases whose scar size were less than 5 mm. Study Limitations: The cure rate of the therapy was 91.2%. Conclusion: The wound natural healing therapy is effective in treating tumor-like hypertrophic scar, which can prevent recurrence and has good cosmetic results.


Subject(s)
Male , Female , Child , Adolescent , Adult , Middle Aged , Aged , Young Adult , Wound Healing/physiology , Cicatrix, Hypertrophic/surgery , Wound Closure Techniques , Postoperative Period , Recurrence , Surgical Wound Infection/etiology , Sutures/adverse effects , Suture Techniques/adverse effects , Treatment Outcome , Cicatrix, Hypertrophic/pathology , Cicatrix, Hypertrophic/prevention & control , Preoperative Period
19.
An. bras. dermatol ; 92(4): 499-504, July-Aug. 2017. tab, graf
Article in English | LILACS (Americas) | ID: biblio-887007

ABSTRACT

Abstract: Background: Venous ulcers represent 70% of the lower limb ulcers. They are difficult to heal, requiring a correct diagnostic and therapeutic approach. Many products have been developed to healing, such as homologous platelet gel obtained from the platelet concentrate exceeding from blood transfusion. Objective: To evaluate the safety and efficacy of homologous platelet gel in venous ulcers compared with hydrocolloid dressing. Method: A pilot randomized clinical trial in patients with venous ulcers. Randomized groups (homologous platelet gel and hydrocolloid groups) were followed for 90 days and were assessed through the evolution of ulcerated area, qualitative analysis of vascularization and adverse events. Both groups used elastic compression. Results: We included 16 participants, with a total of 21 venous ulcers. Both treatments promoted a reduction of the areas of the ulcers in 90 days (mean 69%), there was significant difference between the groups concerning the gradual reduction of the ulcers areas, favorably to the hydrocolloid (70% vs 64%; p <0.01). There were some mild adverse events in both groups. Study limitations: Single-center study with a small number of patients, preventing more accurate assessment of the effects of platelet gel. Conclusion: The homologous platelet gel associated with the elastic compression can be an alternative to the venous ulcer treatment and is safe due to the occurrence of a few mild local adverse events and no serious adverse events. Clinical trials with larger numbers of patients must be performed to maintain the indication of this treatment for venous ulcer.


Subject(s)
Humans , Adolescent , Adult , Middle Aged , Young Adult , Varicose Ulcer/therapy , Wound Healing/physiology , Bandages, Hydrocolloid , Platelet-Rich Plasma , Wound Healing/drug effects , Pilot Projects , Prospective Studies , Treatment Outcome , Bandages, Hydrocolloid/adverse effects , Bandages, Hydrocolloid/standards , Gels
20.
Rev. ADM ; 74(3): 159-162, mayo-jun. 2017.
Article in Spanish | LILACS (Americas) | ID: biblio-908014

ABSTRACT

La bolsa adiposa de Bichat está constituida por un cuerpo y tres extensiones de tejido adiposo, se localiza dentro del espacio bucal y seextiende siguiendo el borde anterosuperior del músculo masetero. En la actualidad su uso como colgajo pediculado ha demostrado excelentesresultados en el tratamiento de reconstrucción de defectos postumorales de tejido blando en el paladar. Lo anterior se debe a su gran aporte vascular, composición histológica y a la presencia de células madre en tejido adiposo que fomentan una metaplasia del tejido, convirtiéndose en tejido fibroso y superfi cialmente con epitelio en tan sólo cinco semanas. La técnica de abordaje y reposicionamiento del colgajo pediculado en paladar fue modifi cada con la extirpación de la tuberosidad del maxilar para corregir el defecto ocasionado por un adenoma pleomorfo en el paladar. Se destacan las características y cualidades de la bolsa adiposa de Bichat para su uso en reconstrucción de defectos tumorales.


Bichat’s buccal fat pad is constituted by a body and three extensions ofadipose tissue within the buccal space and extending to the anteriorsuperiorborder of the masseter muscle. To this days, the use of thebuccal fat pad as a pedicled graft has shown excellent results onoral post tumoral reconstruction treatment. This is due to its greatvascularity, histological composition and perhaps to the presenceof stem cells that promotes a metaplasia, turning adipose tissue intofi brous and superfi cially epithelized tissue within fi ve week aftersurgery. Surgical approach and repositioning technique of the pedicledgraft was modifi ed, extirpating part of the maxillary tuberosity, topreserve vascularity and cover up a hard-soft tissue defect caused by apleomorphic adenoma on a patient’s palate. Buccal fat pad’s qualitiesand characteristics are taken into consideration to demonstrate theeff ectiveness on its surgical reconstructive uses.


Subject(s)
Male , Humans , Adult , Adenoma, Pleomorphic/surgery , Adipose Tissue/transplantation , Palatal Neoplasms/classification , Palatal Neoplasms/surgery , Surgical Flaps , Biopsy/methods , Mexico , Oral Surgical Procedures/methods , Stem Cells/physiology , Wound Healing/physiology
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