Nutrients requirements for Trichoderma reesei and Sclerotium cepivorum as effective factors on value adding wastes

The Trichoderma reesei and Sclerotium cepivorum requirements for cellulases production from beet pulp and olive cake were investigated with respect to their affinity to cellulases activities. The appreciated nutrients were [NH4]2 So4 [1.6 g/l] KH2 P04 [4.0 g/l], Ca CL2 [0.4 g/l], Mg So4 [0.6 g/l], and Tween 80 [2.0 g/l] for T. reesei. However, these concentrations, in the same order, were 1.4, 3.0, 0.3, 0.3, and 2.0 g/l for S. cepivorum with substitution of [NH4]2SO4 by NH4 H2 PO4. Vitamin B mixture seemed to be more suitable for T. reesei, whereas, sodium acetate was the best for S. cepivorum. Addition of 5.0% filter paper in the presence of 2.0% beet pulp resulted in the highest values of cellulase enzymes production and activities for both organisms

Cellulases production from wastes under different conditions

The optimal conditions were tested to achieve the highest enzymes production by fermentation. Beet pulp and olive cake, some of the predominant waste materials in Egypt, were checked as base media with respect to cellulases production. The maximum enzyme activities of cellulases produced by Trichoderma reesei and Sclerotium cepivorum were estimated to verify the optimal conditions. The optimal conditions were, 12 day of incubation period, 30 degree C as optimum temperature, 5.0 as pH value and 4% as inoculum size, for T. reesei. The corresponding values for S. cepivorum were 16 days, 21 degree C, 4.5 and 2%. The optimum shaking rate for T. reesei was 200 rpm