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OBJECTIVE To observe the effects of serum metabolites by using 1H-NMR-based metabonomic approach to explore the possible mechanisms of total flavonoids in Ocimum Basilicum Linn (OBL) on atherosclerosis in apolipomtein E knockout (ApoE-/-) mice. METHODS Six-week-old male apoE knockout mice were divided into four groups(n=10)and fed with high fet diet:model,Simv-astatin, OBL-H, OBL-M and OBL-L groups. The homogeneous male mice of C57BL/6J were used as the normol group and fed with normal chow diet. After 14 weeks,1H-NMR technology was used to ex-plore the variability of serum metabolites by the method of PLS-DA and OPLS-DA. RESULTS Com-pared with normal group,Model group showed a significant increase in the serum levels of VLDL,LDL, β-hydroxyisobutyrate,lactate,myo-inositol and showed a significant decrease in the serum levels of al-anine, glutamine, proline, carnitine, methylamine, citrate, creatine, choline, taurine, pyruvate, β-glu-cose, α-glucose, glycine, lysine. Combined with model group OBL-H, OBL-M, OBL-L groups showed the effects of regulating the levels of different metabolites of the glucose,lipid and amino acid metabo-lism.CONCLUSION The anti-atheros-clerotic activity of total flavonoids in Ocimum Basilicum Linn may be related not only to regulation of lipid metabolism,but also glycometabolism and amino acid metabolism.
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OBJECTIVE 1H-NMR-based metabolomics approach was conducted to holistically explore the effect and mechanisms of Cydonia oblanga Mill flavonoids(COMF)on high-fat diet induced Atherosclerosis (AS) apoE-/- mice. METHODS AS model was established on the apolipoprotein e knockout mice by high-fat diet. The ApoE-/- mice were split into 6 groups including control group, AS model group,COMF High dose(COMF-H)group,COMF medium dose(COMF-M)group,COMF Low dose (COMF-L) group and Simvastatin group as the positive control group. Serum samples from all groups were analyzed by 1H-NMR technology and the OPLS-DA was conducted to distinguish the metabolic phenotypes. RESULTS Compared to the control group, serum levels of cholesterol, VLDL, leucine, isoleucine, valine, blood lipid, citrulline, methylamine, glucose, glycine, glycerol, myo-inositol, fructose, phenylalanine, unsaturated lipid, urea and other metabolites content significantly increased, while HDL, lactate, alanine, glutamate, glutamine, pyruvate, carnitine, citrate, choline content signifi-cantly decreased and the difference was statistically significant (P<0.05). The trend of metabolites in serum samples of COMF low, medium and high group was opposite to that of atherosclerosis model group and the difference was statistically significant(P<0.05).CONCLUSION Through functional analysis of these biomakers,amino acid metabolism,lipid metabolism,cholesterol metabolism,energy metabolism and inflammation reaction were considered as the most relevant pathological biomakers in the serum of AS mice.This study also demonstrates that COMF had the therapeutic effectiveness on AS through partly reversing the lipid,cholesterol,amino acid,energy metabolism and Inflammation reaction.
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OBJECTIVE Using bronchial asthma rats, to observe the effects of Smooth wheezing effect of polysaccharide. In addition, to study the effects of COM in different bronchial asthma model. METHODS Bronchial rats established by ovalbumin (OVA), were randomly divided into different group.Every other week building reference literature intraperitoneal injection of OVA, 21 d after injection of 3 consecutive ultrasonic atomization inhalation of 1% OVA stimulating 30 d,stimulate the 31 d began to medicine.Lavage for 4 weeks ELISA test ratio of IgE,SP-A,IL-4 and IL-5 was serum and bronchoal veolar lavage fluid etc.Data was in x ± s tabular format,SPSS16.0 statistics software is used to perform statistical analysis on the data, and P<0.05 shows meaningful statistical difference. RESULTS Quince polysac-charide can reduce the IgE,IL-4 level and elevated the SP-A,IL-5 level in serum and bronchoal veolar lavage fluid.CONCLUSION Quince polysaccharide has antiasthmatic effect on bronchial asthma rats.