Stable transfection of pEGFP-N1-MOG plasmid to utilize in multiple sclerosis gene therapy

Introduction: Multiple sclerosis [MS] is a disease of the immune system: it attacks the myelin around the axons and leaves them exposed. Destruction of myelin weakens the electrical conduction of ions and thus leads to a lack of communication in the nervous system

Methods: In the present study, we constructed recombinant plasmid and then transformed to E. coli cell. The colonies containing plasmid were selected by Colony PCR. Enzyme digestion and sequencing were utilized to approve the accuracy of the extracted plasmid of these clones. Recombinant plasmid transfect in to mesanchymal stem cells

Results: Plasmid was verified correctly. After transfection, the transcription of MOG gene and the expression of MOG protein were proved by RT-PCR, western blotting and Elisa

Conclusion: Plasmid was constructed correctly and mesenchyme stem cells were successfully transfected by transfection and protein can be expressed well, setting a proper foundation for the future studies on the transplantation of gene modified mesanchymal stem cells in order to promote Multiple sclerosis

[ impact of education on nutritional behavior change among clients of Sardar- Jangal health center in 2012]

Nutrition is one of the effective factors in the protection of health and the prevention of disease. Therefore, determination of the relationship between nutrition choices and health of people is emphasized. The aim of this study is to determine the effectiveness of education and nutrition consultation on changing the unhealthy nutritional habits, and improving healthy nutrition behavior among the clients of Sardar-Jangal health center in 2012. This interventional study was performed with the participation clients of Sardar Jangal health center in 2012. The sample size was 1500 and the sampling method was census. Data was gathered through a questionnaire and interview. The questionnaire included information regarding age, sex, education, employment and nutritional status. The scores between 9-11, 5-8 and less than 5, respectively, were considered as favorable, relatively favorable and unfavorable nutritional status. The participants with relatively favorable and unfavorable status were referred to nutrition education and consultation classes. At the end of the consultation classes, their nutritional status were assessed again. The data was analyzed through SPSS 21, using T-Test. In the study, 1500 clients of Sardar Jangal health center participated. The sample included 383 [25.6%] male and 1117 [74.4%] female. The mean age of male and female was 38.3 +/- 21.1 and 36.9 +/- 17.4 respectively. Before participating in the nutrition education and consultation classes, the nutrition status of 343[30.7%] of females was favorable whilst for 775 [69.3%], the nutritional status was unfavorable. Among 114 [29.8%] of males it was favorable and amongst 268 [70.2%] it was unfavorable. Between the mentioned clients, the nutrition status of 144 [20.1%] females and 28 [12.7%] males improved after nutritional consultation [P < 0.001]. The results showed nutritional education and consultation are effective in improving the nutritional behavior and modifying the incorrect nutritional behavior

Cardiopulmonary bypass, inflammation and how to defy it: focus on pharmacological interventions

One of the most common health problems are diseases of the cardiovascular system with a great bulk of disease burden; while a considerable number of cardiac patients undergo cardiac surgery; cardiac surgical procedures with cardiopulmonary bypass [CPB] are nowadays among the top list of surgical procedures. More than half of a century has passed since the introduction of total cardiopulmonary bypass [CPB]. One of the main untoward effects of CPB is systemic inflammation; causing an [acute phase reaction] responsible for the production of other unwanted postoperative complications. The humoral and cellular components of the immune system are among the main parts of these compensatory mechanisms. There are a number of therapeutic agents used to suppress this inflammatory process. Since CPB is composed of a multitude of items, there are many studies assessing the possible methods and therapeutics for prevention or treatment of inflammation in patients undergoing CPB. According to a conventional classification, the anti-inflammatory methods are classified as either pharmacologic strategies or technical strategies. The pharmacologic strategies are those with the usage of one or more therapeutic agents; while the technical strategies are those that try to modify the CPB techniques. However, in this manuscript, the main pharmacological strategies are discussed

Suitability of MRS-bile agar for the selective enumeration of mixed probiotic bacteria in presence of mesophilic lactic acid cultures and yoghurt bacteria

Measuring the viability of probiotic microorganisms in food products using plate count methodology is a common practice due to the simplicity [ease of performance], inexpensive and routine testing characters of this method. In present study, the suitability of de man rogosa and sharpe agar [MRS] bile agar medium for the selective enumeration of mixed probiotic bacteria [Lactobacillus acidophilus LA-5, L. casei 431 and Bifidobacterium lactis BB-12] in presence of mesophilic lactic cultures [Lactococcus lactis ssp. lactis and Lactococcus lactis ssp. Cremoris] and yoghurt bacteria [Streptococcus thermophilus and Lactobacillus delbrueckii ssp. Bulgaricus] was investigated. Yoghurt bacteria did not grow neither in presence of 0.15% nor 0.30% of bile salts, as was expected. Mesophilic lactic starters could grow at both concentrations of bile salts at all incubation temperatures except 37°C. According to these results, MRS-bile agar [0.15 bile salts] could be successfully used for selective enumeration of mixed probiotic cultures in presence of mesophilic culture and/or yoghurt bacteria when plates were incubated at 37°C for 72 h