use of spectrophotometer in identification of bloodstains: normal haemoglobin and glycosylated hameoglobin

This study was attempted to apply an easy and low-cost method foridentification, differentiation of species of origin, and aging ofbloodstains. There were quite wide variations in the values of thealpha-ratio between bloodstains of different animal species and those of humanbeings. There was a significant negative correlation between themeans of the alpha-ratio and time in all the studied species extracted withboth ammonia and saline. This work showed an absorption spectrum, in thevisible region, of a minute speck of blood, which can prove that the substance under examination is blood. However, no accurate data could be given for thedifferentiation between animal and human blood, or between diabetic andnondiabetic human blood

Glycosylated proteins in vitreous versus blood as a post-mortem diagnostic measure for diabetes mellitus

The present study was carried out on 79 Egyptian cadavers, aged 14-75 years. They were classified into two groups: Group I [n = 49] included cadavers withnegative history of diabetes mellitus, and group II [n = 30] included cadaverswith positive history of diabetes mellitus. Randomly collected blood samplesas well as vitreous samples from both eyes of cadavers were used and laboratory investigations were performed. The data ultimately obtained were analyzed statistically to be used as a simple model system for postmortem diagnosis of diabetes mellitus. It was concluded that the F/A as well as F/P ratios from the vitreous were considered to be better parameters that fructosamine and/or glucose in the postmortem diagnosis of diabetes mellitus. They were less expensive and less time-consuming than detecting fructosamine and/or glucose in serum, which need correction due to hemolysis- induced interferences. Moreover, the vitreous fluid being well isolated, sterile and devoid of blood was much more better than blood in the postmortem diagnosis of diabetes mellitus

Post-mortem diagnosis of renal failure

The present study was carried out on 100 Egyptian subjects, aged 17-75 years. They were classified into 4 groups: Group I [n=20] included healthy subjectswith negative history of renal troubles, group II [n=20] included patientswith previous known history of uremia, and the last group included 60 cadaverswhich again divided into group III [n=18] included cadavers without provedrenal pathology, and group IV [n=24] included cadavers with proved renalpathological changes. Randomly collected blood samples from the wholesubjects as well as vitreous samples from both eyes of cadavers were used forthe estimation of urea, creatinine, uric acid, and G- glutamyl transpeptidaseactivities. In addition, wedge-shaped renal slices from both kidneys weretaken from cadavers to assure the diagnosis, and to make if possible acorrelation between the histopathologic findings and the biochemical results