Nanotopography and oral bacterial adhesion on titanium surfaces: in vitro and in vivo studies

Abstract The present study aimed to evaluate the influence of titanium surface nanotopography on the initial bacterial adhesion process by in vivo and in vitro study models. Titanium disks were produced and characterized according to their surface topography: machined (Ti-M), microtopography (Ti-Micro), and nanotopography (Ti-Nano). For the in vivo study, 18 subjects wore oral acrylic splints containing 2 disks from each group for 24 h (n = 36). After this period, the disks were removed from the splints and evaluated by microbial culture method, scanning electron microscopy (SEM), and qPCR for quantification of Streptococcus oralis, Actinomyces naeslundii, Fusobacterium nucleatum, as well as total bacteria. For the in vitro study, adhesion tests were performed with the species S. oralis and A. naeslundii for 24 h. Data were compared by ANOVA, with Tukey's post-test. Regarding the in vivo study, both the total aerobic and total anaerobic bacteria counts were similar among groups (p > 0.05). In qPCR, there was no difference among groups of bacteria adhered to the disks (p > 0.05), except for A. naeslundii, which was found in lower proportions in the Ti-Nano group (p < 0.05). In the SEM analysis, the groups had a similar bacterial distribution, with a predominance of cocci and few bacilli. In the in vitro study, there was no difference in the adhesion profile for S. oralis and A. naeslundii after 24 h of biofilm formation (p > 0.05). Thus, we conclude that micro- and nanotopography do not affect bacterial adhesion, considering an initial period of biofilm formation.

Marginal gap of zirconia and lithium disilicate frameworks produced by the CAD-CAM technique through a comparator microscope ­ in vitro analysis / Fenda marginal de estruturas de zircônia e dissilicato de lítio produzidas pela técnica CAD-CAM através de um microscópio comparador ­ análise in vitro

Objective: The aim of this study was to evaluate the marginal gap of frameworks produced using the CAD-CAM system, from zirconia and lithium disilicate blocks, adapted to a tooth preparation and a gypsum die. Material and Methods: For this study, a human first molar tooth was used as a master model with a full crown preparation. It was molded 20 times to obtain the gypsum die and randomly divided into 2 groups (n=10) for the fabrication of zirconia and lithium disilicate frameworks. The frameworks were made using pre-sintered zirconia blocks and lithium disilicate blocks, both CAD-CAM systems. The marginal gap was measured in µm at four points (buccal, palatal, mesial, and distal) using a comparator microscope with 30x magnification, with the framework seated on the master model (tooth), and on the gypsum die. Marginal gap data (µm) were evaluated using two-way analysis of variance and Tukey's test with a significance level of 5%. Results: The results showed that there was no statistically significant interaction between the factors studied (p=0.223) or isolated factors (ceramic factor p=0.886 and die factor p=0.786). Conclusion: Both ceramics produced using the CAD-CAM technique did not exhibit statistical differences in marginal adaptation on the two types of substrates, both on tooth preparation and on the gypsum die (AU)

Objetivo: O objetivo deste estudo foi avaliar o espaço marginal de estruturas produzidas usando o sistema CAD-CAM, a partir de blocos de zircônia e dissilicato de lítio, adaptadas a um preparo sobre dente e a um troquel de gesso. Material e Métodos: Para este estudo, um dente molar humano foi utilizado como modelo mestre com preparo para coroa total. Este foi moldado 20 vezes para obter o troquel de gesso e dividido aleatoriamente em 2 grupos (n=10) para a fabricação de estruturas de zircônia e dissilicato de lítio. As estruturas foram feitas usando blocos de zircônia pré-sinterizados e blocos de dissilicato de lítio, ambos sistemas para CAD-CAM. O espaço marginal foi medido em µm, em quatro pontos (bucal, palatal, mesial e distal), utilizando um microscópio comparador com ×30 de ampliação e com a estrutura assentada no modelo mestre (dente) e no troquel de gesso. Os dados de espaço marginal (µm) foram avaliados usando análise de variância bidirecional e teste de Tukey com um nível de significância de 5%. Resultados: Os resultados mostraram que não houve interação estatisticamente significativa entre os fatores estudados (p=0,223) ou isoladamente (fator cerâmica p=0,886 e fator troquel p=0,786). Conclusão: Ambas as cerâmicas produzidas usando a técnica CAD-CAM não apresentaram diferenças estatísticas em relação à adaptação marginal nos dois tipos de substratos, tanto na preparação dentária quanto no troquel de gesso(AU)

Microtensile bond strength of resin composite to dentin using different adhesive systems and directions of electric current

Resumo O objetivo neste estudo foi avaliar o efeito da direção da corrente elétrica na resistência da união resina composta-dentina usando três sistemas adesivos. Dentes molares humanos foram distribuídos de acordo com o sistema adesivo (dois passos autocondicionante - Clearfil SE Bond, Kuraray [CSE]; e um passo autocondicionante - Single Bond Universal, 3M ESPE [SBU]; e dois passos convencional - Adper Single Bond 2, 3M ESPE [SB2]), a direção da corrente elétrica (sem corrente elétrica - controle, correntes elétricas direta e reversa - 35µA) e tempo de armazenamento (24h - imediato e 6 meses). Blocos de resina composta (Filtek Z350XT, 3M ESPE) foram aderidos à dentina. Amostras de dentina-resina foram produzidas e armazenadas em água destilada a 37ºC por 24 horas e 6 meses para o teste de resistência da união à microtração (µTBS) (n = 10; ~12 palitos por dente). Os padrões de fratura foram analisados ​​em estereomicroscópio e classificados em falhas coesiva na dentina, coesiva na resina, adesiva ou mista. A penetração do adesivo na dentina e a formação da camada híbrida foram avaliadas em microscópio eletrônico de varredura (MEV). Os dados foram submetidos à ANOVA três fatores seguidos pelo teste post hoc de Tukey (α = 0,05). Não houve diferenças na µTBS quando os sistemas adesivos foram aplicados sob as correntes elétricas direta e reversa, mas ambas as correntes elétricas aumentaram a µTBS para todos os sistemas adesivos. SBU apresentou os menores valores de µTBS para o grupo controle em ambos os tempos de armazenamento e para a corrente elétrica direta em 6 meses de armazenamento. Falhas adesivas foram mais frequente em todos os grupos. A corrente elétrica formou longos tags resinosos para todos os sistemas adesivos. O armazenamento por 6 meses não diminuiu significativamente os valores de µTBS. Ambos os sentidos da corrente elétrica (cargas positivas e negativas) a 35µA podem aumentar a µTBS dos sistemas adesivos testados à dentina.

Abstract Thisstudy aimed to evaluate the effect of the electric current direction application on the resin composite-dentin bond strength using three adhesive systems. Human molar teeth were distributed according to the adhesive system (two-step self-etch - Clearfil SE Bond, Kuraray [CSE]; one-step self-etch - Single Bond Universal, 3M ESPE [SBU]; and two-step etch-and-rinse - Adper Single Bond 2, 3M ESPE [SB2]), electric current direction (without electric current - control, direct and reverse electric currents - 35µA), and storage time (24h - immediate and 6 months). Resin composite blocks (Filtek Z350XT, 3M ESPE) were bonded to dentin. The teeth/resin composites specimens were stored in distilled water at 37ºC for 24 hours and 6 months for the microtensile bond strength (µTBS) test (n = 10; ~12 sticks for each tooth). Failure patterns were analyzed on a stereomicroscope and classified as cohesive-dentin, cohesive-resin, adhesive or mixed. Adhesive penetration into dentin and hybrid layer formation were evaluated in a scanning electron microscope (n = 6). Data were submitted to a three-way ANOVA followed by Tukey's post hoc test (α = 0.05). There are no differences in µTBS when the adhesive systems were applied under direct and reverse electric currents, but both electric currents increased the µTBS for all adhesive systems. SBU showed the lowest µTBS values for control groups in both storage times and direct electric current in 6 months of storage. The adhesive failure pattern was more frequently observed in all groups. The electric current formed long resin tags for all adhesive systems. Storage for 6 months did not significantly decrease µTBS values. Both directions of electric current (positive and negative charges) at 35µA can increase the µTBS of the adhesive systems tested to dentin.

Influence of angulation and height of tooth preparation on the distribution of tensions in prosthetic crowns for upper central incisor: in silico analysis

Aim: it was to use tridimensional finite element analysis (FEA) to analyze the effect of height and angulation of prosthetic preparations on the distribution of stresses for lithium disilicate prosthetic crowns, the underlying resin cement, and the prosthetic preparation of a superior central incisor. Methods: a CAD modeling software, SolidWorks 2013, was used to generate three-dimensional virtual models comprising the dimensions of the preparation parameters. Three angles (6, 12 and 16 degrees) were simulated on the prepared walls and two wall heights were utilized (4 and 5 mm), for a total of six model groups according to the height and angulation of the walls. A vertical line in the Y-axis was used as a reference for determining the degrees of convergence (inclination of the preparations). The chamfer finish and preparation width were standardized for all groups. Results: the 4 mm preparations behaved more appropriately when the axial wall convergence was approximately 6 degrees. The 5 mm preparations required 12 degrees of angulation. In relation to resin cement, there was better stress distribution when the angle of incisal convergence was between 6 and 12 degrees. An increase to 16 degrees led to a considerable increase in peak stress at the preparation margin. Conclusion: it was concluded that the convergence of the axial walls of coronal preparations with 4 and 5 mm heights should be 6 and 12 degrees, respectively, to avoid high tension spikes in the underlying resin cement

Evaluation of dimensional stability and details reproduction of alginate molds storage in different times and temperature / Avaliação da estabilidade dimensional e reprodução de detalhes de moldes de alginatos armazenados por diferentes tempos e temperatura

Objective: The aim of this study was to evaluate dimensional stability and detail reproduction in alginate molds stored at different times and temperatures. Material and Methods: Three different commercially available alginates (Cavex Color Change, Jeltrate Plus and Hydrogum 5) were tested at four different times (0 - control, 1, 3 and 5 days) and two temperatures (25ºC and 37ºC) (n=5). The alginates were handled following the manufacturer's instructions. The impression procedures occurred in an environment with controlled room temperature (25ºC) and relative humidity (50±5%). A metallic model (ISO 1563:1990) was used to perform the impressions. The tray containing alginate remained on the metallic model under constant pressure (2 kgf) until the alginate gelation process. The molds were stored (different times and temperatures) and analyzed in stereomicroscope at 30x magnification and 0.5 µm accuracy (Olympus Measuring Microscope STM). The molds and the metallic model measurements were compared, and the data were statistically analyzed by Kolmogorov-Smirnov and three-way ANOVA tests, and the means were compared by Tukey test (5%). Results: The results showed no double interactions (p>0.05) and the different alginates presented statistically similar values of dimensional stability (p=0.102). However, 25ºC (temperature) and control group (time) showed the highest values of dimensional stability (p <0.05). Jeltrate Plus was the only material that presented inaccuracy in details reproduction. Conclusion: It is recommended that, for tested alginates, the stone casts should be poured immediately, even though the molds are dimensionally stable up to 5 days when stored at 25ºC.(AU)

Objetivo: O objetivo neste estudo foi avaliar a estabilidade dimensional e a reprodução de detalhes em moldes de alginatos armazenados em diferentes tempos e temperaturas. Material e Métodos: Foram testadas três diferentes marcas de alginato (Cavex ColorChange, Jeltrate Plus e Hydrogum 5), sendo que esses materiais foram armazenados por quatro diferentes tempos (0 - controle, 1, 3 e 5 dias) e duas diferentes temperaturas (25ºC e 37ºC) (n=5). Os alginatos foram manipulados seguindo as instruções do fabricante. A moldagem ocorreu em um ambiente com temperatura (25ºC) e umidade relativa (50±5%) controladas. Foi utilizado um modelo metálico (ISO 1563:1990) para a realização das moldagens. A moldeira contendo o alginato permaneceu sobre o modelo metálico sob pressão constante de 2 kgf até a geleificação do material. Em seguida, os moldes foram armazenados nos tempos e temperaturas testados, sendo posteriormente avaliados sob um microscópio comparador (Olympus Measuring Microscope STM) com precisão de 0,5 µm. As medidas dos moldes de alginato foram comparadas com as medidas do modelo metálico padrão, sendo os dados analisados estatisticamente pelos testes de Kolmogorov-Smirnov e ANOVA três fatores, sendo as médias foram comparadas pelo teste de Tukey (5%). Resultados: Os resultados mostraram nenhuma interação dupla (p>0,05), sendo que os diferentes alginatos apresentaram valores de estabilidade dimensional estatisticamente semelhantes entre si (p=0,102). Já a temperatura de 25ºC e o grupo controle (tempo) mostraram os maiores valores de estabilidade dimensional (p <0,05). O Jeltrate Plus foi o único material que apresentou imprecisão na reprodução de detalhes. Conclusão: Recomenda-se que os alginatos testados tenham os modelos de gesso vazados imediatamente, apesar dos moldes serem dimensionalmente estáveis até 5 dias desde que armazenados a 25ºC. (AU)

Influence of Different Concentration and Ratio of a Photoinitiator System on the Properties of Experimental Resin Composites

Abstract The aim in this study was to evaluate the influence of different ratio of camphorquinone/tertiary amine concentration on the flexural strength (FS), elastic modulus (EM), degree of conversion (DC), yellowing (YL), water sorption (WS) and water solubility (WSL) of experimental composites. Thus, acrylate blends were prepared with different camphorquinone (CQ) and amine (DABE) concentrations and ratios by weight: (CQ/DABE%): 0.4/0.4% (C1), 0.4/0.8% (C2), 0.6/0.6% (C3), 0.6/1.2% (C4), 0.8/0.8% (C5), 0.8/1.6% (C6), 1.0/1.0% (C7), 1.0/2.0% (C8), 1.5/1.5% (C9), 1.5/3.0% (C10). For the FS and EM, rectangular specimens (7x2x1 mm, n=10) were photo-activated by single-peak LED for 20 s and tested at Instron (0.5 mm/min). Then, the same specimens were evaluated by FTIR for DC measurement. For YL, disks (5x2 mm, n=10) were prepared, light-cured for 20 s and evaluated in spectrophotometer using the b aspect of the CIEL*a*b* system. For WS and WSL, the volume of the samples was calculated (mm³). For WS and WSL, composites disks (5x0.5 mm, n=5) were prepared. After desiccation, the specimens were stored in distilled water for 7 days and again desiccated, in order to measure the WS and WSL. Data were submitted to one-way ANOVA and Tukey's test (5%). The groups C8, C9 and C10 showed higher DC, EM and YL means, compared to other composites. Therefore, the FS and WS values were similar among all groups. Also, C1, C2 and C3 presented higher WSL in 7 days, compared to other composites. In general, higher concentrations of camphorquinone promoted higher physical-mechanical properties; however, inducing higher yellowing effect for the experimental composites

Resumo O objetivo foi avaliar a influência da concentração e proporção de canforoquinona (CQ)/amina terciária (DABE) na resistência à flexão (RF), módulo flexural (MF), grau de conversão (GC), amarelamento (AM), sorpção (SA) e solubilidade em água (SL) de compósitos experimentais. Blendas acrilatas foram preparadas com diferentes concentrações e proporções de CQ/DABE em peso, como segue: (CQ/DABE%): 0,4/0,4% (C1); 0,4/0,8% (C2); 0,6/0,6% (C3); 0,6/1,2% (C4); 0,8/0,8% (C5); 0,8/1,6% (C6); 1,0/1,0% (C7); 1,0/2,0% (C8); 1,5/1,5% (C9); 1,5/3,0% (C10). Para RF e MF, espécimes retangulares (7x2x1 mm, n=10) foram fotoativados com LED de pico único (Radii Cal) por 20 s e testados em máquina Instron (0,5 mm/min). Após, o GC dos mesmos espécimes (fragmentos) foi mensurado por FTIR. Para AM, discos de compósito (5x2 mm, n=10) foram preparados, fotoativados por 20 s e imediatamente avaliados em espectrofotômetro, considerando o aspecto b do sistema CIEL*a*b*. Para SA e SL, discos de compósito (5x0,5 mm, n=5) foram preparados e seus volumes calculados (mm³). Após desidratação, as amostras foram pesadas e armazenadas em água destilada por 7 dias, pesadas e novamente desidratadas e pesadas, para se calcular o SA e SL. Os dados foram submetidos a one-way ANOVA e teste de Tukey (5%). Os grupos C8, C9 e C10 mostraram maior GC, MF e AM, comparado aos outros grupos. No entanto, RF e SA foram similares entre todos os grupos. Ainda, C1, C2 e C3 apresentaram maior SL em 7 dias comparado aos outros grupos. Em geral, maiores concentrações de CQ promoveram melhores propriedades físico-mecânicas; no entanto, levaram ao maior amarelamento dos compósitos.

Dimensional accuracy of different impression techniques of partially edentulous mandibular arch / Precisão dimensional de diferentes técnicas de moldagem de uma mandíbula parcialmente edêntulo

ABSTRACT Objective: The purpose of this in vitro study was to assess and compare the dimensional accuracy of three impression techniques: 1-step putty/light-body, 2-step putty/light-body, and the monophase technique. Methods: A partially edentulous standard stainless steel mandibular arch cast with reference points on the teeth was used to make the impressions. The anteroposterior and transverse distances were measured. All impressions were made with a polyvinyl siloxane using stock metallic (1- and 2-step putty/light-body techniques) or acrylic resin (monophase technique) trays. The monophase impressions were made using a light-body material and the 1- and 2-step putty/light-body impressions with putty and light-body materials. After impression procedures, the accuracy of each technique was assessed measuring the stone casts (n = 5) poured from the impressions using a microscope at 30x magnification and at 0.5 µm accuracy. The data were analyzed statistically using 2-way ANOVA and Tukey's test (p<0.05). Results: Stone casts made by all techniques had significantly negative linear changes (shrinkage). The anteroposterior distances showed more dimensional changes than the transverse distances. The edentulous side showed more shrinkage than the anteroposterior side. Conclusion: No differences between the impression techniques were found, but significant dimensional changes were observed.

RESUMO Objetivo: Avaliar e comparar a precisão dimensional de três técnicas de moldagem: dupla mistura, reembasamento e moldeira individual. Métodos: Um modelo metálico inferior parcialmente edêntulo foi fabricado com pontos de referência nos dentes e usado para fazer as moldagens. As distâncias ântero-posteriores e transversais foram medidas. Todas as moldagens foram feitas com um silicone por adição utilizando moldeiras metálicas (técnicas da dupla mistura e reembasamento) ou de resina acrílica (técnica da moldeira individual). Na técnica da moldeira individual foi utilizado apenas o elastômero de viscosidade leve e nas técnicas da dupla mistura e reembasamento foram usados os elastômeros nas viscosidades denso e leve. Após os procedimentos de moldagem, a precisão dimensional de cada técnica de moldagem foi avaliada aferindo os modelos de gesso (n = 5) em um microscópio comparador com 30x de aumento e 0,5 µm de precisão. Os dados foram analisados estatisticamente por ANOVA dois fatores e teste de Tukey (p<0,05). Resultados: Os modelos de gesso feitos com todas as técnicas de moldagem apresentaram alterações lineares negativas (contração). As distâncias ântero-posteriores apresentaram maiores alterações dimensionais que as distâncias transversais. O lado parcialmente edêntulo teve maior contração que o lado ântero-posterior oposto. Conclusão: Não foram encontradas diferenças entre as técnicas de moldagem, mas foram observadas significantes alterações dimensionais.

Evaluation of phenyl-propanedione on yellowing and chemical-mechanical properties of experimental dental resin-based materials

ABSTRACT Objective To evaluate the influence of phenyl-propanedione on yellowing and chemical-mechanical properties of experimental resin-based materials photoactivated using different light curing units (LCUs). Material and Methods Experimental resin-based materials with the same organic matrix (60:40 wt% BisGMA:TEGDMA) were mechanically blended using a centrifugal mixing device. To this blend, different photoinitiator systems were added in equimolar concentrations with aliphatic amine doubled by wt%: 0.4 wt% CQ; 0.38 wt% PPD; or 0.2 wt% CQ and 0.19 wt% PPD. The degree of conversion (DC), flexural strength (FS), Young’s modulus (YM), Knoop hardness (KNH), crosslinking density (CLD), and yellowing (Y) were evaluated (n=10). All samples were light cured with the following LCUs: a halogen lamp (XL 2500), a monowave LED (Radii), or a polywave LED (Valo) with 16 J/cm2. The results were analysed by two-way ANOVA and Tukey’s test (α=0.05). Results No statistical differences were found between the different photoinitiator systems to KNH, CLS, FS, and YM properties (p≥0.05). PPD/CQ association showed the higher DC values compared with CQ and PPD isolated systems when photoactivated by a polywave LED (p≤0.05). Y values were highest for the CQ compared with the PPD systems (p≤0.05). Conclusion PPD isolated system promoted similar chemical and mechanical properties and less yellowing compared with the CQ isolated system, regardless of the LCU used.

Accuracy of partially edentulous arch impressions obtained from different alginates and storage times

Purposes: This study aimed to assess the dimensional accuracy of five commercial alginates verified in stone casts. Methods: Each alginate impression (Cavex ColorChange, Cavex Holland BV; Jeltrate Plus, Dentsply; Hydrogum, Zhermack; Kromopan 100, Lascod; Ezact Kromm, Vigodent) was performed on partially edentulous standard stainless steel mandibular arch cast with reference points on teeth 33, 43, 37 and 47. On the stainless steel cast, the anteroposterior (33-37 and 43-47) and transverse (33-43 and 37-47) distances were measured in a stereomicroscope at 30x magnification and 0.5 µm accuracy. The distances between these points were measured three times, obtaining an average, which was analyzed statistically and compared with the distances obtained from the stone casts. For each alginate the casts were poured gypsum (n = 5) immediately and after a period of 1, 2, 3 and 5 days of the impression procedure. Results: The dimensional accuracy values of stone and stainless steel casts were analyzed statistically by two-way ANOVA and Tukey's test (α=0.05). The results showed significant differences between the alginates; however, no differences in dimensional accuracy were found among the different storage times of alginate impression. Conclusions: It can be concluded that the alginate impressions can be stored for up to 5 days (AU)

Influência dos sistemas fotoiniciadores sobre as propriedades físicas e mecânicas de cimentos resinosos fotoativados por diferentes fontes de luz / Effect of light curing units on the physical and mechanical properties of lutting resin cements containing different photoinitiators systems

A utilização de sistemas iniciadores alternativos na composição de cimentos resinosos fotoativados tem por objetivo melhorar as propriedades químicas, mecânicas e estéticas dos agentes de cimentação odontológicos, reduzindo os aspectos desfavoráveis do sistema iniciador convencional, canforoquinona/amina. Para isso, um correto relacionamento entre o espectro de emissão da fonte de luz e o espectro de absorção do fotoiniciador é fundamental. O presente estudo tem como objetivo, através de uma revisão de literatura, avaliar as possíveis alterações físicas e mecânicas atribuídas a agentes de cimentação resinosos fotoativados, contendo diferentes sistemas fotoiniciadores sob efeito de diferentes tipos de fonte de luz...

The use of alternative initiator systems in the composition of luting resin cements aims to improve chemical, mechanical, and aesthetic properties of dental cementation agents, reducing the drawbacks aspects of the conventional initiator system, canphorquinone/amine. Therefore the proper interaction between the light-curing units emission spectrum and the photo-initiator absorption spectrum is r. This study presents a literature review with the objective of evaluating the possible physical and mechanical changes on photoactivated resin cements, containing different photo-iniciators systems under the effect of different types of light sources...

Influence of Photoinitiator and Light-Curing Source on Bond Strength of Experimental Resin Cements to Dentin

Abstract This study evaluated the bond strength (BS) of experimental resin cements formulated with different photoinitiators when activated by two kinds of light-curing units (LCUs) through a ceramic material. Seven resin blends with different camphorquinone (CQ) and/or phenylpropanedione (PPD) concentrations (weight) were prepared: C5: 0.5% CQ; C8: 0.8% CQ; P5: 0.5% PPD; P8: 0.8% PPD; C1P4: 0.1% CQ and 0.4% PPD; C4P1: 0.4% CQ and 0.1% PPD; C4P4: 0.4% CQ and 0.4% PPD. Two LCUs were used: one quartz-tungsten-halogen (QTH - 850 mW/cm²) and one light-emitting diode (LED - 1300 mW/cm²). The microtensile bond strength of each blend was assessed. Data were submitted to two-way ANOVA and Tukey's test (α=0.05). The BS values did not exhibit significant differences for LCUs, regardless of the photoinitiator type. Three cements showed significant differences: P5 and C5 had higher BS with QTH, and C4P1 with LED. For QTH, P5 showed the highest and C1P4 the lowest BS. For the LED, C4P1 showed the highest BS of all the cements. The results indicated that PPD was a viable alternative in the formulation of photocured resin cements, reducing or eliminating CQ that is yellowish without impairing the bond strength. Furthermore, both LED and QTH were effective in curing resin cements that contain PPD or CQ.

Resumo Este estudo avaliou a resistência de união (BS) de cimentos resinosos experimentais formulados com diferentes fotoiniciadores, quando ativados através de cerâmica, por dois tipos de fontes luminosas (LCUs). Foram preparadas sete formulações com diferentes concentrações em peso (wt) de canforquinona (CQ) e/ou fenilpropanodiona (PPD): C5: 0,5%CQ; C8: 0,8%CQ; P5: 0,5%PPD; P8: 0,8%PPD; C1P4: 0,1%CQ e 0.4%PPD; C4P1: 0,4%CQ e 0,1%PPD; C4P4: 0,4%CQ e 0,4%PPD. Duas LCUs foram usadas: uma com luz halógena (QTH - 850 mW/cm²) e uma com diodo emissor de luz (LED - 1300 mW/cm²). A BS foi avaliada por teste de microtração. Os dados foram submetidos a ANOVA a dois fatores e Teste de Tukey (α=0,05). Isolando o tipo de fotoiniciador, não houve diferenças significativas na BS. Três cimentos mostraram diferenças significativas: a BS foi maior para P5 e C5 com QTH; e para C4P1 com LED. Para a QTH, P5 exibiu a maior e C1P4 a menor BS. Para LED, C4P1 exibiu a maior BS de todos os outros cimentos testados. Conclusão: PPD é altamente viável em formulações de cimentos resinosos fotopolimerizáveis, reduzindo ou eliminando a CQ que é amarelada, sem comprometer a resistência de união. Além disso, tanto LED quanto QTH são efetivas para polimerizar os cimentos contendo PPD ou CQ.

Procedimentos Cirúrgicos Minimamente Traumáticos em Periodontia: Revisão de Literatura / Minimally Traumatic Surgical Procedures in Periodontics: a Review

O conceito de procedimentos minimamente invasivos pode ser estendido a todas as áreas da odontologia, mas a periodontia, em especial, temresultados benéficos quando da utilização desta modalidade terapêutica. Dessa forma, esta revisão de literatura teve como objetivo descrevera utilização dos procedimentos minimamente invasivos em cirurgias periodontais, seus conceitos, aplicações e possiveis beneficios. Para estarevisão de literatura, foram avaliados 682 artigos publicados entre 1950 e 2012 que abordaram o tema cirurgia periodontal minimamenteinvasiva. Destes 682 artigos, 668 não descreveram claramente os procedimentos realizados não atendendo aos critérios de inclusão e portantoforam descartados. Cuidados como iluminação adequada promovem um aumento da acuidade visual durante os procedimentos cirurgicos,assim favorecendo a precisão associada aos instrumentos microcirúrgicos especificamente concebidos e permitindo uma manipulação maisprecisa e minimamente traumática dos tecidos moles e duros. Além disso, auxilia na melhora do acesso cirúrgico, evita a remoção desnecessáriade tecidos, a optimização do desbridamento, melhora a vascularização e, por conseguinte, a possibilidade de obtenção de cicatrizaçãoprimária de feridas cirúrgicas. A abordagem microcirúrgica pode melhorar a previsibilidade dos diferentes procedimentos periodontais,proporcionar melhores resultados estéticos e causar menor desconforto pós-operatório. No entanto, poucos estudos foram realizados commetodologias estabelecidas sobre o uso de instrumentos que promovam procedimentos minimamente invasivos em periodontia. Portanto, sãonecessários estudos para determinar se as técnicas microcirúrgicas podem levar a uma diferença significativa no prognóstico da terapeuticaproposta. A maioria dos estudos encontrados na literatura são baseados em observações e experiências dos autores, que mostram claramenteque há vantagens em uma melhor iluminação, melhor visão e técnica mais controlada e menos traumática.

The concept of minimally invasive procedures can be extended to all s of dentistry. Periodontics, in particular, has been reported as oneof the areas with great benefits. This review aims to describe the use of minimally invasive procedures in periodontal surgery, its concepts,applications, and possible benefits from its use. For that, 682 articles published between 1950 and 2012 focused on minimally invasiveperiodontal surgery were evaluated. Of them, 669 studies did not describe clearly the procedures, and did not attend the inclusion criteria.The results showed that proper lighting promotes increased visual acuity during surgical procedures, favoring the precision associated withmicrosurgical instruments specifically designed, allowing a more accurate manipulation of the soft and hard tissues. Surgical access avoidsunnecessary tissues removal, optimizing the debridement, improving vascularisation, and therefore the possibility of obtaining primary healingof surgical wounds. The microsurgical approach can improve the predictability of different periodontal procedures, providing better resultsand cause less postoperative discomfort. However, few controlled methodologies on the use of instruments to promote minimally invasiveprocedures in periodontics have been found in literature. Therefore, studies are needed to determine whether microsurgical techniques can leadto a significant difference in the successful outcome. Most of the studies are based on observations and experiences of the authors, which clearlyshow that there are advantages in having better lighting, better vision, and a more controlled and less traumatic technique.

Influence of photoinitiators and light sources on the degree of conversion of experimental resin cements / Influência de fotoiniciadores e fontes de luz no grau de conversão de cimentos resinosos experimentais

Objetivo: O objetivo deste estudo foi avaliar o grau de conversão (DC) de sete cimentos resinosos experimentais formulados com diferentes fotoiniciadores, quando ativados por duas fontes luminosas (LCUs) atravessando a cerâmica. Material e Métodos: Sete formulações foram preparadas com diferentes concentrações (em peso) de Canforoquinona (CQ) e/ou Fenilpropanodiona (PPD): C5: 0,5% CQ; C8: 0,8% CQ; P5: 0,5% PPD; P8: 0,8% PPD; C1P4: 0,1% CQ e 0,4% PPD; C4P1: 0,4% CQ e 0,1% PPD; e C4P4: 0,4% CQ e 0,4% PPD. Cada mistura foi carregada com 65% em peso de partículas de carga silanizada. Foram usadas duas fontes luminosas para a fotoativação: uma de luz halógena de quartzo-tungstênio (QTH) e uma de diodo emissor de luz (LED). A irradiância (mW/cm²) foi calculada pela potência média de saída dividida pela área da ponta do aparelho. DC foi obtido por espectroscopia de infravermelho transformada de Fourier. Os dados foram submetidos a ANOVA 2-Fatores e ao teste de Tukey (5%). Resultados: Os valores de DC não exibiram diferenças significativas para LCUs, independente do tipo de fotoiniciador. O maior DC foi observado para o cimento P8, e o menor para C5. Valores intermediários de DC foram observados nos demais cimentos. No entanto, quando usado QTH, P8 diferiu dos cimentos C1P4, C4P1 e C5; e quando o LED foi empregado, P8 diferiu apenas de C4P1 e C5. Conclusão: Portanto, PPD é uma alternativa viável para o desenvolvimento de cimentos resinosos fotoativados, e a associação PPD/CQ também pode ser viável, uma vez que C4P4 foi semelhante a P8.

Objective: The aim of this study was to evaluate the degree of conversion (DC) ofseven experimentalresin cements formulated with different photoinitiators when activated by two light-curing units (LCUs) through ceramic material. Material and Methods: Seven resin blends with different camphorquinone (CQ) and/or phenyl propanedione (PPD) ratios were prepared: C5: 0.5% wt CQ; C8: 0.8% wt CQ; P5: 0.5% wt PPD; P8: 0.8% wt PPD; C1P4: 0.1% wt CQ and 0.4% wt PPD; C4P1: 0.4% wt CQ and 0.1% wt PPD; and C4P4: 0.4% wt CQ and 0.4% wt PPD. Each mixture was loaded with 65% wt of silanized filler particles. For photoactivation procedures, two LCUs were used: a quartz-tungsten-halogen (QTH) and a light emitting diode (LED). Irradiance (mW/ cm²) was calculated by the ratio of the output power by the area of the tip. DC was assessed by Fourier transformed infrared spectroscopy. Data were submitted to a two-way ANOVA and Tukey’s test (5%). Results: DC values do not show significant differences for LCUs regardless of the photoinitiator type. The highest DC was found for experimental cement P8 and the lowest for C5. Intermediate DC values were found for the other cements. However, when QTH was used, P8 exhibited differences among C1P4, C4P1 and C5; whereas when LED was used, P8 differed only for C4P1 and C5. Conclusion: Thus, PPD is a viable alternative for the manufacture of photoactivated cements, and the PPD/CQ association may also be viable since C4P4 was similar to P8.

Fotoativação na atualidade: conceitos e técnicas clínicas / Photoactivation in actuality: concepts and clinical techniques

A odontologia adesiva tem guiado bastante a reabilitação estética dos dentes, especialmente no que se refere às restaurações diretas e indiretas. Desse modo, os materiais resinosos (adesivos, resinas compostas e cimentos resinosos), fotoativados ou duais, são bastante utilizados na clínica odontológica e dependem de maneira essencial de correta fotoativação. Vários são os aparelhos fotoativadores e as técnicas de fotoativação, fazendo com que o dentista necessite saber qual fonte de luz e técnica são mais adequadas para a situação clínica em questão. O objetivo do presente estudo é apresentar os conceitos sobre fotoativação e aparelhos fotoativadores, bem como as técnicas clínicas para sua aplicação nos materiais resinosos utilizados na odontologia

Adhesive dentistry has guided the esthetic rehabilitation of the teeth, especially with direct and indirect restorations. In this sense, resin materials (adhesives, resin composites and resin cements), photoactivated or dual-cured, are often used in dental clinics and depend on an adequate photocuring. There are many light curing and photoactivation techniques, causing the dentist the need to know which source of light and technique are most appropriate for the clinical situation in question. Thus, the aim of the present work is to show the concepts of photocuring and light curing units, as well as photoactivation techniques for the resin materials used in dentistry

Influência da Irrigação na Osseointegração: Revisão da Literatura / Osseointegration Influenced by the Irrigation System: a Literature Review

O uso de implantes para a reabilitação oral é pratica comum na odontologia. Entretanto, falhas são relatadas na literatura. Para evitar o insucesso, cuidados na preparação do leito receptor dos implantes são importantes e, entre esses cuidados, o não aquecimento do osso adjacente é fundamental. A elevação da temperatura durante a osteotomia é um fator importante para o fracasso da técnica cirúrgica. O objetivo do trabalho foi revisar a literatura sobre a influência da irrigação na osseointegração. Foram realizadas buscas em bases de dados no portal de periódicos da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), National Center for Biotechnology Information (NCBI)/U.S., National Library of Medicine (PubMed) e Google Acadêmico, no perído de 1964 a 2012. Foram selecionados 333 estudos relacionados à busca, sendo que 203 foram descartados por não abordarem de forma clara a relação entre o controle do calor gerado e o procedimento de instalação de implantes orais. Os 130 artigos remanescentes foram selecionados e 50 estudos foram incluídos. Foram identificados os fatores relacionados aos diferentes sistemas de irrigação do tipo externo e do tipo interno usados para o controle da temperatura, tanto das brocas como do tecido ósseo adjacente, durante a osteotomia para a inserção de implantes. Baseado na literatura, é imprescindível a utilização da irrigação durante a confecção dos alvéolos cirúrgicos para a inserção de implantes dentários, independentemente de serem interno ou externo, já que os estudos mostram que o desempenho de ambos é semelhante, salvo em casos específicos, como no caso de cirurgias guiadas, em que a irrigação interna parece ser mais eficiente.

The use of implants for oral rehabilitation is a common practice in dentistry, though failures are reported in literature. To avoid failure, careful preparation of the recipient site of the implants is important, and among these cares, not heating the adjacent bone is essential. Increasing temperature during osteotomy is an important factor in failure of the surgical technique. This study aimed to review the literature about the influence of irrigation on osseointegration. Searches were conducted in databases on the portal of periodicals from Coordination of Improvement of Higher Education Personnel (CAPES), National Center for Biotechnology Information (NCBI) / U.S., National Library of Medicine (PubMed) and Google Scholar, in the period from 1964 to 2012. Then, 333 studies were selected, but 203 were discarded for not clearly addressing the subject. One hundred and thirty remaining articles were selected, and 50 studies were included. The study identified the factors related to the external and internal irrigation systems used to control the temperature of drills and adjacent bone tissue during the osteotomy for the insertion of implants. Based on the literature the irrigation during the surgical preparation for the insertion of dental implants is fundamental, independently from being internal or external systems, once the performance of both is similar, except in specific cases, including guided surgery in which the internal irrigation appears to be more efficient.

Photoelastic evaluation of the effect of composite formulation on polymerization shrinkage stress

We compared polymerization stress in two commercial composites and three experimental composites made using camphorquinone (CQ) and/or phenylpropanedione (PPD) as photoinitiators. The internal surfaces of photoelastic resin discs with cylindrical cavities were roughened and treated with adhesive. Composites were divided into five groups: two commercial composites (Filtek Silorane and Filtek Z250) and three experimental composites with CQ/amine, CQ/PPD/amine, and PPD/amine. Composites were photopolymerized inside cavities, and subjected to photoelastic analysis immediately and at 24 hours and 7 days later using a plane polariscope. Stress created by Silorane (3.08 ± 0.09 MPa) was similar to that of Z250 (3.19 ± 0.13 MPa) immediately after photopolymerization (p > 0.05). After 24 hours and 7 days, Z250 (3.53 ± 0.15 and 3.69 ± 0.10 MPa, respectively) showed higher stress than Silorane (3.19 ± 0.10 and 3.16 ± 0.10 MPa, respectively). Qualitative analysis immediately after photopolymerization showed composite/CQ promoted higher stress than PPD, but stress levels at other evaluated times were statistically similar, varying between 3.45 ± 0.11 MPa and 3.92 ± 0.13 MPa. At 24 hours and 7 days, Silorane created the lowest stress. All photoinitiators created comparable tensions during polymerization.

Effect of different photo-initiators and light curing units on degree of conversion of composites

The aim of this study was to evaluate: (i) the absorption of photo-initiators and emission spectra of light curing units (LCUs); and (ii) the degree of conversion (DC) of experimental composites formulated with different photo-initiators when activated by different LCUs. Blends of BisGMA, UDMA, BisEMA and TEGDMA with camphorquinone (CQ) and/ or 1-phenyl-1,2-propanedione (PPD) were prepared. Dimethylaminoethyl methacrylate (DMAEMA) was used as co-initiator. Each mixture was loaded with 65 wt percent of silanated filler particles. One quartz-tungsten-halogen - QTH (XL 2500, 3M/ESPE) and two lightemitting diode (LED) LCUs (UltraBlue IS, DMC and UltraLume LED 5, Ultradent) were used for activation procedures. Irradiance (mW/cm²) was calculated by the ratio of the output power by the area of the tip, and spectral distribution with a spectrometer (USB 2000). The absorption curve of each photo-initiator was determined using a spectrophotometer (Varian Cary 5G). DC was assessed by Fourier transformed infrared spectroscopy. Data were submitted to two-way ANOVA and Tukey's test (5 percent). No significant difference was found for DC values when using LED LCUs regardless of the photo-initiator type. However, PPD showed significantly lower DC values than composites with CQ when irradiated with QTH. PPD produced DC values similar to those of CQ, but it was dependent on the LCU type.

Grau de conversão e densidade de ligações cruzadas de compósitos formulados com diferentes fotoiniciadores / Degree of conversion and cross-link density of resin composites formulated with different photoinitiators

O estudo verificou a influência da fonte de luz (FL) e do sistema fotoiniciador no grau de conversão (GC) e na densidade de ligações cruzadas (DLC) em compósitos com diferentes fotoiniciadores. Foram utilizadas as FLs XL2500® (3M/ESPE), Apollo95E® (DMD), UltraBlueIS® (DMC) e UltraLume5® (Ultradent), todas de acordo com as recomendações dos fabricantes e com 19J de energia aplicada, e os compósitos Pyramid-Bisco® (contendo fotoiniciador Lucirin TPO) e Filtek® Z250-3M/ESPE (fotoiniciador canforoquinona-CQ). O GC foi mensurado por espectroscopia de infra-vermelho transformada de Fourier. A DLC foi medida indiretamente por meio da leitura de dureza Knoop antes e após banho em etanol. Os dados foram submetidos à Anova e as médias, ao teste de Tukey (5%). Apollo 95E® produziu os maiores valores de GC para o compósito TPO (60,2%) quando a dose de energia foi mantida constante. A FL Ultra-BlueIS® não foi capaz de fotoativar o compósito TPO. Não existiram diferenças nos valores de GC para ocompósito CQ, a não ser quando Apollo 95E® foi usado pelo tempo recomendado pelo fabricante, levando a menor média (50,8%) de GC. Em geral, Ultra Lume5® produziu maior DLC, e Apollo 95E®, menor DLC para ocompósito TPO; para o compósito CQ não houve um padrão para os valores de DLC. Conclui-se que fontes de luz e fotoiniciadores influenciam as propriedades de compósitos dentais fotoativados.

Influence of light-curing units on the flexural strength and flexural modulus of different resin composites

Aim: This study aimed to evaluate the flexural strength and flexural modulus of different resin composites (MasterFill, Opallis, Z250, Supreme) after photoactivation with quartz-tungsten-halogen (QTH - XL2500) or light-emitting diode (LED - Radii and Ultrablue Is) light-curing units (LCUs). Methods: The irradiance and spectral emission of each unit were evaluated using a power meter and a spectrometer. Flexural strength (MPa) was determined in a three-point bending test in accordance with the ISO4049 standard specifications. Flexural modulus (GPa) was measured from the linear-elastic range on the stress-deformation profile. Data were submitted to two-way ANOVA and Tukey’s test (p<0.05). Results: Different values for irradiance (850, 780 and 590 mW/cm2) and peak of emission (484, 456, 467 nm) were detected for XL2500, Radii and Ultrablue Is, respectively. Flexural strength and modulus were dependent on both material and LCU. Among the resins, Z250 and Supreme showed significantly higher strength and modulus than MasterFill and Opallis. Comparing the LCUs, Ultrablue Is showed significantly lower flexural strength and modulus than the others. Conclusion: Flexural strength and modulus were dependent on the irradiance and the spectral emission of the curing units, as well on the resin composite tested.

In vitro toothbrushing abrasion of dental resin composites: packable, microhybrid, nanohybrid and microfilled materials

This study evaluated weight loss and surface roughening after toothbrushing of different resin composites: one packable (Solitaire 2, Heraeus Kulzer), one microhybrid (Charisma, Heraeus Kulzer), one nanohybrid (Simile, Pentron) and one microfilled (Durafill VS, Heraeus Kulzer). Cylindrical specimens (n = 20) were prepared. Half of the samples were submitted to 60,000 strokes, at 4 Hz, with a dentifrice-water slurry. Control samples (n = 10) remained stored at 37°C. Pre- and post-abrasion parameters for weight (mg) and surface roughness (Ra, µm) were determined on an analytical balance and a surface profilometer. Data were separately submitted to Repeated Measures ANOVA and Tukey's test (a = 0.05). Percentages of weight loss were analyzed by ANOVA and Tukey's test (a = 0.05). The relationship between both evaluations was assessed by Pearson's test (a = 0.05). The means ( percent) for weight loss (standard deviation) were 0.65(0.2), 0.93(0.2), 1.25(0.6) and 1.25(0.4) for Simile, Durafill, Charisma and Solitaire, respectively. Baseline roughness means ranged from 0.065(0.01), 0.071(0.01), 0.066(0.02) and 0.074(0.01) for Simile, Durafill, Charisma and Solitaire, respectively, to 0.105(0.04), 0.117(0.03), 0.161(0.03) and 0.214(0.07) after testing. The composites with larger fillers presented higher weight loss and roughening than the finer materials (p < 0.05). For both evaluations, control specimens showed no significant alteration. No significant relationship between loss of weight and roughness alteration was detected (r = 0.322, p = 0.429).