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Chinese Journal of Medical Genetics ; (6): 436-437, 2003.
Artículo en Chino | WPRIM | ID: wpr-329441

RESUMEN

<p><b>OBJECTIVE</b>To detect the methylenetetrahydrofolate reductase(MTHFR) gene C677T mutation with molecular beacon technique and assess the revant applicability.</p><p><b>METHODS</b>A total of 228 samples were analyzed using molecular beacons which are oligonucleotide probes to become fluorescent upon hybridization. Wild-type molecular beacon and mutant beacon were designed to detect the genotypes of MTHFR gene.</p><p><b>RESULTS</b>Analysis of the 228 samples indicated that there were three genotypes including 41 homozygous mutants, 113 heterozygous individuals and 74 wild-type individuals. Every sample was identified clearly.</p><p><b>CONCLUSION</b>The present method, a closed-tube PCR/hybridization assay, is a simple, high-throughput and fast procedure that is fully automated for detecting gene mutation.</p>


Asunto(s)
Humanos , Análisis Mutacional de ADN , Métodos , Colorantes Fluorescentes , Química , Genotipo , Metilenotetrahidrofolato Reductasa (NADPH2) , Genética , Sondas de Oligonucleótidos , Química , Genética , Mutación Puntual , Sensibilidad y Especificidad
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