RESUMEN
BACKGROUND: This study was to investigate the effect of biomechanical stimulation on osteoblast differentiation of human periosteal-derived stem cell using the newly developed bioreactor. METHODS: Human periosteal-derived stem cells were harvested from the mandible during the extraction of an impacted third molar. Using the new bioreactor, 4% cyclic equibiaxial tension force (0.5 Hz) was applied for 2 and 8 h on the stem cells and cultured for 3, 7, and 14 days on the osteogenic medium. Biochemical changes of the osteoblasts after the biomechanical stimulation were investigated. No treatment group was referred to as control group. RESULTS: Alkaline phosphatase (ALP) activity and ALP messenger RNA (mRNA) expression level were higher in the strain group than those in the control group. The osteocalcin and osteonectin mRNA expressions were higher in the strain group compared to those in the control group on days 7 and 14. The vascular endothelial growth factor (VEGF) mRNA expression was higher in the strain group in comparison to that in the control group. Concentration of alizarin red S corresponding to calcium content was higher in the strain group than in the control group. CONCLUSIONS: The study suggests that cyclic tension force could influence the osteoblast differentiation of periosteal-derived stem cells under optimal stimulation condition and the force could be applicable for tissue engineering.
Asunto(s)
Humanos , Fosfatasa Alcalina , Reactores Biológicos , Calcio , Maxilares , Mandíbula , Tercer Molar , Osteoblastos , Osteocalcina , Osteonectina , ARN Mensajero , Células Madre , Ingeniería de Tejidos , Factor A de Crecimiento Endotelial VascularRESUMEN
OBJECTIVES: The aim of the present study was to evaluate the stability of non-thermal atmospheric-pressure plasma on Candida albicans in hairless mouse-2 (HRM-2) tissues. METHODS: HRM-2 mice were subjected to non-thermal atmospheric-pressure plasma jet treatment using an optical fiber probe and monitored using a thermometer. The skin of HRM-2 mice was treated with plasma jet for 0, 60, 180, and 300 s per day for 5 days. After plasma treatment, morphological changes in Candida albicans on the skin of these mice were examined using a scanning electron microscope. Biopsy of the plasma-treated skin was performed and the tissues were histologically analyzed using hematoxylin and eosin (H&E) and Masson's trichrome stains. RESULTS: The scanning electron microscopic images revealed the morphological changes in the membrane structure of the plasma-treated Candida albicans. Histological analysis showed that non-thermal plasma treatment did not cause epidermal damage or tissue inflammation and did not significantly modify the collagen layers of the mouse skin. CONCLUSIONS: The results of this study suggest that non-thermal atmospheric-pressure plasma might be safe and effective for clinical applications in the field of dentistry.
Asunto(s)
Animales , Ratones , Biopsia , Candida albicans , Colágeno , Colorantes , Odontología , Eosina Amarillenta-(YS) , Hematoxilina , Inflamación , Membranas , Ratones Pelados , Microscopía Electrónica de Rastreo , Fibras Ópticas , Gases em Plasma , Plasma , Piel , TermómetrosRESUMEN
We correct a typo in the title.