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Objective To analyze the relationship between bone mineral density (BMD) of infants over 6 months of age and exclusive breastfeeding and calcium nutrition guidance during pregnancy in Baoding area, and to provide evidence for clinical application. Methods A total of 308 infants over 6 months of age were selected from Baoding Maternal and Child Health Hospital from January 2020 to January 2023, and their BMD was measured by ultrasound. The level of 25 (OH) D3 in subjects' blood was detected. spearman correlation test was used to analyze the correlation between infant bone mineral density and exclusive breastfeeding and calcium nutritional guidance during pregnancy, and logistics regression model was used to analyze the independent factors affecting infant bone mineral density. Results The level of serum 25 (OH) D3 in normal BMD group was significantly higher than that in abnormal BMD group (P<0.05). The rate of exclusive breastfeeding and the guidance rate of calcium nutrition during pregnancy in normal BMD group were significantly higher than those in abnormal BMD group (P<0.05). There was a significant positive correlation between different bone mineral density and exclusive breastfeeding and calcium nutrition guidance during pregnancy (P<0.05). Serum 25 (OH) D3 level, exclusive breastfeeding rate and calcium nutritional guideline rate during pregnancy were independent protective factors for bone mineral density (P<0.05). Conclusion Bone mineral density (BMD) of infants over 6 months of age is positively correlated with exclusive breastfeeding and calcium nutrition guidance during pregnancy, and exclusive breastfeeding and calcium nutrition guidance during pregnancy are independent protective factors affecting BMD of infants over 6 months of age.
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Objective To establish a method for simultaneous determination of seven active ingredients(Jasminoidin,Forsythoside A,Caffeotannic acid,3,5-Di-O-caffeoylquinic acid,Jatrorrhizine Hydrochloride,Baicalin,Berberine Hydrochloride).Methods The determination was carried out with Agilent Poroshell 120 SB-C18(100 mm ×3.0 mm,2.7 μm);The mobile phase consisted of acetonitrile(A)-0.1% phosphoric acid solution(B)with gradient elution at a flow rate of 1.0 mL/min;The detection wavelengths were 230 nm in 0~4.5 min(determination of Jasminoidin),348 nm in 4.5 ~9.0 min(determination of Forsythoside A,Caffeotannic acid,3,5-Di-O-caffeoylquinic acid),and 278 nm in 9.0 ~15.0 min (determination of Jatrorrhizine Hydrochloride;Baicalin;Berberine Hydrochloride).Results The calibration curves were linear in the ranges of 4.024~80.48μg/mL for Jasminoidin(r=0.9991),2.042~40.84μg/mL for Forsythoside A(r=0.9993),3.956 ~79.12 μg/mL for Caffeotannic acid(r=0.9996),6.090 ~121.80μg/mL for 3,5-Di-O-caffeoylquinic acid(r=0.9990),4.026 ~80.52 μg/mL for Jatrorrhizine Hydrochloride(r=0.9995),3.924~78.48 μg/mL for Baicalin(r=0.9998),6.030~120.6μg/mL for Berberine Hydrochloride(r=0.9998).The average recoveries(n=6)were 96.5%,97.5%, 95.9%,98.6%,99.8%,98.4% and 97.3% ;RSDs were 1.5%,1.3%,1.2%,1.4%,1.2%,1.1% and 0.9% respectively.Conclusion The method is simple and repeatable,which can be applied to the quality control of Xiongju Shangqing pills.