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Genet. mol. biol ; 35(1): 95-98, 2012. ilus, tab
Artículo en Inglés | LILACS | ID: lil-616999

RESUMEN

In the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. The Bubu-DRB sequence showed maximum homology with the BoLA-DRB3*0101 allele of cattle. A total of seven amino acid residues were found to be unique for the Bubu-DRB sequence. The majority of amino acid substitutions was observed in the β1 domain. Residues associated with important functions were mostly conserved. Water buffalo DRB was phylogenetically closer to goat DRB*A.


Asunto(s)
Animales , Búfalos , Diclororribofuranosil Benzoimidazol , ADN Complementario , Genes MHC Clase I
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