RESUMEN
OBJECTIVE:To establish the method for simultaneous determination of liquiritin and glycyrrhizic acid in Fuzi lizhong pills (condensed pills).METHODS:HPLC method was adopted.The determination was performed on WondaSil C18 column with mobile phase consisted of acetonitfile-0.05％ phosphate solution (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 237 nm,and column temperature was 35 ℃.The sample size was 10 μL.RESULTS:The linear range ofliquiritin and glycyrrhizic acid were 9.68-96.8 μg/mL(r=0.999 1)and 14.08-140.8 μg/mL(r=0.999 2).The limits of quantitation were 0.2,0.3 μg/mL,and the limits of detection were 0.1,0.01 μg/mL.RSDs of precision,stability and reproducibility tests were all lower than 2.0％.The average recoveries were 98.9％-101.2％ (RSD=0.62％,n=9),98.6％-101.5％ (RSD=1.06％,n=9).CONCLUSIONS:The method is simple and accurate,and can be used for simultaneous determination of liquiritin and glycyrrhizic acid in Fuzi lizhong pills (condensed pills).