Histological evaluation of inflammation after sealing furcation perforation in Dog's teeth by four materials

The materials used in sealing furcating perforation can have considerable effects on controlling the ensuing inflammation and periodontal repair. The objective of the present study was to carry out a histological comparison between the effects of pro-root, cold ceramic, glassionomer cement, and root MTA on the healing of periodontal tissues after furcal perforation in dog's teeth. One-hundred premolar teeth of one-year old dogs were used in this experimental/animal study. After anesthetizing the dogs and the premolar teeth, the access cavities were prepared at the occlusal level and the root canals were instrumented and filled with gutta percha and AH26 sealer, using the step-back technique. Furcations were perforated to a size of 3X3 mm2, using long burs. These areas were then randomly filled with aforementioned four test materials [a total number of 84 premolar teeth] while the access cavities were filled with amalgam. The remaining 16 teeth were selected to serve as positive and negative controls. Biopsy samples were taken from the perforated areas at 1, 2, and 3-month intervals and were transferred to laboratory for pathological examination. The results were statistically analyzed, using the Kruskal Wallis and Mann Whitney tests. The statistical analysis revealed that under similar conditions, periodontal tissues surrounding Pro-root, show less inflammatory response than the other three materials. However, no significant differences were observed among the four studied materials during 1 and 2months as evidenced by the biopsy samples [P>0.05]. For longer period [three month], however, samples surrounding cold ceramic and Root MTA showed decreasing inflammatory responses. From the findings of the present study, it may be concluded that although tissues adjacent to Proroot showed less inflammatory response than other three test materials, all of them [Proroot, Glass-ionomer cement, cold ceramic, and Root MTA] may be considered to be suitable materials for sealing furcal perforation providing. They receive approval by other tests including micro leakage, cytotoxicity, tissue analysis, and etc

[Combined uses of two kinds of LASERS Nd-YAG and Er-YAG in treatment of oral fibroma and pathological evaluation of obtainedsamples: case report]

Today, the laser is used in a wide range of dental procedures. Some of the advantages of some lasers in treatment of oral soft tissue lesions are: elimination of need for local anesthesia, minimal bleeding, fast healing, absence of postoperative complications and reduction of recurrence rate. For these reasons is was used for treatment of oral fibroma. To our knowledge this is the first time both Nd: YAG and Er: YAG lasers was used for biopsy of oral fibroma in Iran. Oral fibroma was removed by these two lasers and examined by pathologist. The patient had no pain without using any local anesthesia. While using Nd: YAG laser, bleeding was not seen but cutting of tissue was slower than using blade . When using Er: YAG, cutting of tissue was faster than previous laser but bleeding could be seen