RESUMEN
Objective: Pulegone [PGN] is a monoterpene ketone, whose metabolites exert several cytotoxic effects in various tissues. The present study was conducted in order to evaluate the [R]-[+] PGN-induced alterations in ovarian aromatization, proto-oncogenes and estrogen receptoralpha [ER alpha] and ER beta receptors expressions
Materials and Methods: In this experimental study, mature albino mice were divided into experimental [received 25 mg/kg, 50 mg/kg and 100 mg/kg PGN, orally for 35 days] and control [received 2% solution of Tween 80 as a PGN solvent, orally] groups. The mRNA levels of Er alpha, Er beta, p53, Bcl-2, and cytochrome p450 [Cyp19] as well as ovarian angiogenesis were analyzed through reverse transcription polymerase chain reaction and immunohistochemical techniques, respectively. Moreover, apoptosis of follicular cells, serum estrogen and progesterone levels and mRNA damage were investigated via using terminal transferase and biotin-16-dUTP staining, electrochemilunescence and fluorescent microscopy methods, respectively
Results: The PGN reduced Er alpha, Er beta and Cyp19 expression at 50 mg/kg and 100 mg/kg doses, while significantly elevating p53 and reducing Bcl-2 expression. Finally, PGN impaired ovarian angiogenesis, increased apoptosis, elevated follicular atresia and reduced serum levels of estrogen and progesterone
Conclusion: Chronic exposure to PGN [50 mg/kg and 100 mg/kg], severely affects ovarian aromatization, proto- oncogenes mRNA levels and expression of ERs
RESUMEN
Background: Pulegone as principal component of essential oil, reported to have antibacterial, antioxidant and anti-inflammatory properties. The present study was aimed to evaluate wound healing activity of pulegone in a rat model. Method: Forty rats were used for excisional and incisional wound healing models. For each model twenty male white Wistar rats were randomly divided into five groups (n = 4) of control (CG), Sham surgery, E1, E2 and E3. Wound size, hydroxyproline content of wound and biomechanical testing were assessed. Result: In E2 animals, the wound size was reduced earlier than in E1 and E2 groups (P = 0.035). However, time had significant effect on wound contraction of all wounds. Hydroxyproline contents in the groups CG, sham surgery, E1, E2 and E3 were found to be 51.25 ± 3.40, 58.41 ± 4.62, 68.59 ± 3.53, 86.32 ± 3.18, and 74.26 ± 4.73 mg g-1, respectively. Hydroxyproline contents were increased significantly in E2 compared to E1 and E3 which implied more collagen deposition compared to other experimental groups (P = 0.001). The biomechanical indices, maximum stored energy, stiffness, ultimate strength and yield strength obtained for E2 group were significantly higher than those obtained for E1 and E2 groups (P = 0.002). Conclusion: The pulegone showed a reproducible wound healing potential in rats.
RESUMEN
Background and Objectives: Diarrhea, characterized by increased defecation of wet stool and abdominal pains, is of great importance in public health and is one of the most common causes of morbidity and mortality in children worldwide. In the present study, the effect of Glycyrrhiza glabra as a native medicinal plant on diarrhea was investigated
Material and Methods: The effect of hydro-alcoholic extract of G. glabra [HEGG] on small intestinal transit was studied in overnight fasted mice [23-28 g]. The antidiarrheal activity of extract was evaluated against castor oil induced diarrhea. Further, the effect of extract on castor oil-induced intestinal transit, and enteropooling at the different doses [50 - 200 mg/kg] were evaluated. Loperamide was used as the positive control
Results: HEGG significantly [p< 0.05] delayed the onset of diarrhea in mice. The extracts also showed significant [P<0.05] inhibitor activity against severity of castor oil induced diarrhea and castor oil induced enteropooling in mice when tested at 200 mg/kg. The oral LD50 values obtained were greater than 2240mg/kg in mice
Conclusion: These findings suggest that the hydro-alcoholic extract of G. glabra roots may contain some biologically active ingredients possessing antidiarrhea properties due to inhibition of gastrointestinal propulsion and fluid secretion