Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 3 de 3
Filtrar
Añadir filtros








Intervalo de año
1.
Artículo en Inglés | IMSEAR | ID: sea-25091

RESUMEN

We describe the separation of an active glutamate dehydrogenase [GDH (NADP+)] enzyme from the plasma of patients with P. falciparum infection using columns of sepharose anti-GDH (NADP+) of Proteus spp. The activity of this enzyme was also detected in P. falciparum culture supernatant. The parasitic origin of this enzyme was suggested by western blot analysis using anti-P. falciparum culture supernatant and anti-whole parasite antibodies. The differential inhibition of the P. falciparum GDH (NADP+) indicates that some epitopes recognised by the antibodies in both preparations may be different. The determination of P. falciparum GDH (NADP+) activity could be developed into a specific technique for the diagnosis of falciparum malaria.


Asunto(s)
Animales , Glutamato Deshidrogenasa/sangre , Humanos , Malaria Falciparum/sangre , Plasmodium falciparum/enzimología
2.
Braz. j. med. biol. res ; 31(9): 1149-55, sept. 1998. ilus, graf
Artículo en Inglés | LILACS | ID: lil-222963

RESUMEN

The major aim of this study was to characterize a soluble Plasmodium falciparum antigen from the plasma of malaria-infected humans and Plasmodium falciparum culture supernatants, using immunoabsorbent techniques and Western blotting. An Mr 60-kDa protein was isolated from the plasma of patients with Plasmodium falciparum malaria by affinity chromatography using rabbit anti-Proteus spp GDH(NADP+) serum as ligand. This protein, present in plasma of patients with acute Plasmodium falciparum infection, in Plasmodium falciparum culture supernatants, and in immune complexes, was tested with Plasmodium falciparum malaria hyperimmune serum from patients living in hyperendemic areas and rabbit anti-Proteus spp GDH(NADP+) serum prepared in the laboratory. In this report, we describe the results of a study showing that parasite GDH(NADP+) can be used to detect the presence of Plasmodium falciparum. It appears that this technique permits the chromatographic detection of a Plasmodium falciparum excretion antigen that may be used in the production of monoclonal antibodies to improve immunodiagnostic assays for the detection of antigenemia, and opens the possibility of its use as a non-microscopic screening method.


Asunto(s)
Humanos , Animales , Conejos , Antígenos de Protozoos/sangre , Glutamato Deshidrogenasa/sangre , Malaria Falciparum/enzimología , Plasmodium falciparum/enzimología , Enfermedad Aguda , Anticuerpos Antibacterianos/sangre , Anticuerpos Monoclonales , Western Blotting , Cromatografía de Afinidad , Medios de Cultivo , Glutamato Deshidrogenasa/inmunología , Glutamato Deshidrogenasa/aislamiento & purificación , Inmunoglobulina G/sangre , Técnicas de Inmunoadsorción , Malaria Falciparum/sangre , Malaria Falciparum/parasitología , Plasmodium falciparum/aislamiento & purificación , Solubilidad
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA