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1.
China Journal of Orthopaedics and Traumatology ; (12): 639-641, 2013.
Artículo en Chino | WPRIM | ID: wpr-353052

RESUMEN

<p><b>OBJECTIVE</b>To study the replantation methods and clinical results of amputated fingertip.</p><p><b>METHODS</b>From October 2007 to June 2011, 18 fingers of 13 cases were replanted with anastomosis of palm vein and retaining the nail, including 9 males and 4 females,with an average age of 26 years old ranging from 17 to 45 years old. The time from injury to therapy was from 30 min to 5 h, time of broken finger ischemia was from 1.5 to 7 h. All broken fingers were preservation under normal temperature.</p><p><b>RESULTS</b>All fingers were survived, no vascular crisis happened. All cases were followed up from 3 to 24 months with an average of 14 months. The length and shape of replanted fingers were similar to that of the healthy side. The new nails were smooth, the function was perfect,the sense of pain and touched sensation had been recovered. Their two-piont discriminations ranged from 3 to 6 mm with an average of 5 mm. According to the assessment standard of Chinese Medical Association of Hand Surgery, the results were excellent in 14 cases, good in 3 cases, poor in 1 case.</p><p><b>CONCLUSION</b>Fingertip replantation with anastomosis of palm vein and retaining the nail is regained satisfactory appearance and function of the digits with a high survival rate.</p>


Asunto(s)
Adolescente , Adulto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Adulto Joven , Anastomosis Quirúrgica , Métodos , Dedos , Cirugía General , Mano , Uñas , Cirugía General , Reimplantación , Métodos , Venas , Cirugía General
2.
Chinese Journal of Medical Genetics ; (6): 436-437, 2003.
Artículo en Chino | WPRIM | ID: wpr-329441

RESUMEN

<p><b>OBJECTIVE</b>To detect the methylenetetrahydrofolate reductase(MTHFR) gene C677T mutation with molecular beacon technique and assess the revant applicability.</p><p><b>METHODS</b>A total of 228 samples were analyzed using molecular beacons which are oligonucleotide probes to become fluorescent upon hybridization. Wild-type molecular beacon and mutant beacon were designed to detect the genotypes of MTHFR gene.</p><p><b>RESULTS</b>Analysis of the 228 samples indicated that there were three genotypes including 41 homozygous mutants, 113 heterozygous individuals and 74 wild-type individuals. Every sample was identified clearly.</p><p><b>CONCLUSION</b>The present method, a closed-tube PCR/hybridization assay, is a simple, high-throughput and fast procedure that is fully automated for detecting gene mutation.</p>


Asunto(s)
Humanos , Análisis Mutacional de ADN , Métodos , Colorantes Fluorescentes , Química , Genotipo , Metilenotetrahidrofolato Reductasa (NADPH2) , Genética , Sondas de Oligonucleótidos , Química , Genética , Mutación Puntual , Sensibilidad y Especificidad
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