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Biomedical and Environmental Sciences ; (12): 391-395, 2014.
Artículo en Inglés | WPRIM | ID: wpr-270589

RESUMEN

The effect of sterilization methods on biological activity of fibronectin on the surface of biomaterials was elaborated in the present study. Sterile protein- modified biomaterials were fabricated by microfilter filtration and UV irradiation, respectively. UV irradiation altered the conformation of surface- adsorbed fibronectin and further affected the attachment, morphology and biological function of endothelial cells. However, microfilter filtration did not to change the normal conformation of fibronectin, or the proliferation and biological function of endothelial cells, indicating that microfilter filtration sterilization is the most suitable method for protein-substrate.


Asunto(s)
Adhesión Celular , Efectos de la Radiación , Fibronectinas , Efectos de la Radiación , Filtración , Prótesis e Implantes , Microbiología , Esterilización , Métodos , Rayos Ultravioleta
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