RESUMEN
Background: stress in infancy has dramatic effects on different systems, including the nervous system, endocrine, immune, reproductive and etc
Objective: the purpose of this study was to investigate the effects of extract of Iranian propolis [EIP] on ovarian tissue and oxidative stress in rats with maternal separation stress
Materials and Methods: 48 immature female rats were divided randomly into six groups. 1] Control group, 2] Control group+saline, 3] Stress group, includes infants that were separated from their mothers 6 hr/day, the 4[th], 5[th] and 6[th] groups consisted of infants who in addition to daily stress received 50, 100 and 200 mg/kg of EIP, respectively. Then serum corticosterone, 17-beta-estradiol, malondialdehyde, total superoxide dismutase, glutathione peroxidase and ferric reducing antioxidant power levels were measured. The ovarian sections were stained by H and E, PAS, and TUNEL methods and were studied with optical microscopy
Results: stress increased the blood serum corticosterone levels and 17-beta-estradiol reduced significantly [p<0.001] and EIP prevented from this changes [p<0.01]. EIP significantly increased the number of ovarian follicles, oocytes and oocytes diameter in neonatal rat following stress [p<0.01]. EIP also significantly decreased the number of atretic follicles, TUNEL+granulosa cells, malondialdehyde levels and increased ferric reducing antioxidant power, total superoxide dismutase and glutathione peroxidase serum levels in neonatal rats following stress. The dose of 200 mg/kg EIP was more effective
Conclusion: this Study showed that the Iranian Propolis significantly could prevent oxidative stress and histopathological changes in the ovary of the neonatal rat the following stress
RESUMEN
Objective: Different cryoprotectants are used for cryopreservation of ovarian tissue in patients at risk of infertility. Ethylene glycol [EG], dimethyl sulfoxide [DMSO] and propanediol [PROH] have been chosen as the basic permeable cryoprotectants due to their decreased glass-formation characteristics compared to other cryoprotectants. In the present study, the effects of two different vitrification methods on whole mouse ovarian tissue by the use of a novel staining method [trypan blue] has been evaluated
Methods: Ovaries of 8 day-old NMRI mice were isolated and divided among the control, vitrification 1 [Vit1] and vitrification 2 [Vit2] groups. The Vit1 solution was composed of alpha-MEM+ 20% FBS + 15% EG + 15% DMSO. The Vit 2 solution was composed of alpha- MEM+ 15% FBS +20% EG + 20% PROH. Vit1 and Vit2 procedures were performed at 4°C and room temperature, respectively. Warming was performed in alpha-MEM+ 20% FBS supplemented with 1M sucrose in the Vit1 group and alpha-MEM+ 15% FBS with descending concentrations of sucrose [1, 0.5, 0.25 M] in the Vit2 group. Control and vitrified warmed ovaries were put in alpha-MEM supplemented by 0.4% trypan blue for 20 min, and then stained ovaries were fixed in Bouin's fixative, serially sectioned in paraffin wax and finally quantitatively evaluated under a light microscope
Results: The highest percentage of primordial follicles was observed in the control group. There was a significant difference between the control and Vit1 groups, and between the Vit1 and Vit2 groups [p<0.05]. No significant difference was observed in primary and preantral follicles between the control and vitrification groups
Conclusion: Vitrification with EG and PROH are more suitable for preservation of follicle reserves in ovaries. Trypan blue staining is a faster and easier method for evaluation of ovarian tissue
RESUMEN
Diabetic neuropathy is the most common complication of diabetes mellitus affecting the nervous system. In this study, we investigated the in vivo effects of combined administration of 4-methylcatechol [4-MC] and progesterone [P] as a potential therapeutic tool for sciatic nerve function improvement and its role in histomorphological alterations in diabetic neuropathy in rats. Male adult rats were divided into 3 groups: sham operated control [CO], untreated diabetic [DM] and diabetic treated with progesterone and 4-methylcatechol [DMP4MC] groups. Diabetes was induced by a single dose injection of 55 mg/ kg streptozotocin [STZ]. Four weeks after the STZ administration, the DMP4MC group was treated with P and 4-MC for 6 weeks. Then, following anesthesia, the animals' sciatic nerves were removed and processed for light and transmission electron microscopy [TEM] as well as histological evaluation, Diabetic rats showed a statistically significant reduction in motor nerve conduction velocity [MNCV], nerve blood flow [NBF], mean myelinated fiber [MF] diameters and myelin sheath thickness of the sciatic nerve after 10 weeks. In the sciatic nerve of the untreated diabetic group, endoneurial edema and increased number of myelinated fibers with myelin abnormalities such as infolding into the axoplasm, irregularity of fibers and alteration in myelin compaction were also observed. Treatment of diabetic rats with a combination of P and 4-MC significantly increased MNCV and NBF and prevented endoneurial edema and all myelin abnormalities. Our findings indicated that co-administration of P and 4-MC may prevent sciatic nerve dysfunction and histomorphological alterations in experimental diabetic neuropathy