Effects of Bamboo Salt with Sodium Fluoride on the Prevention of Dental Caries / 치위생과학회지

BACKGROUND: Dental caries is one of several prevalent oral diseases caused by dental plaque biofilms. This study evaluated the anti-cariogenic effects of a bamboo salt (BS) and sodium fluoride (NaF) mixture on oral bacteria.METHODS: The effects of several mixtures of NaF and BS on acid production, growth, and adhesion to glass beads of Streptococcus mutans, and their anti-cariogenic properties were investigated. The growth of S. mutans was measured according to optical density at 3, 6, 9, 12, 15, 18, and 24 hours after treatment using spectrophotometry at a wavelength of 600 nm, while pH was measured using a pH meter. Adhesion of S. mutans was measured according to the weight of glass beads from each group before and after incubation. Gene expression was measured using real-time polymerase chain reaction. Acid production and growth patterns of S. mutans were compared using repeated measures analysis of variance, followed by Scheffe's post-hoc test. The Kruskal–Wallis test was used to compare adhesion, followed by the Mann–Whitney test. Gene expression in the experimental and control samples was compared using the Student's t-test.RESULTS: Growth, acid production, and adhesion of S. mutans were inhibited in all experimental groups. Expression of gft and fructosyltransferase in S. mutans was inhibited in all groups. A mixture of NaF and BS significantly reduced growth, acid production, adhesion, and gene expression of S. mutans compared with the other groups.CONCLUSION: Results of the present study demonstrated that a mixture of NaF and BS was useful as a mouth rinse in preventing dental caries.

The Effect of Digestive Medicine on Enamel Erosion / 치위생과학회지

Consumption of liquid digestive medicine has continually grown in recent years. This present study was designed to evaluate the capability of liquid digestive medicine to erode dental enamel, relating the pH and titratable acidity of liquid digestive medicine. Three commercially available liquid digestive medicines were chosen these were Gashwalmyeungsu, Saengrokchun and Wicheongsu. The liquid digestive medicines were evaluated in respect to pH, titratable acidity and concentrations of calcium and phosphate, respectively. This measure was carried out three times for each digestive medicine and was recorded the data as mean (standard deviation). Bovine enamel specimens measured microhardness at base line and then were randomly assigned to 4 groups of 10 each. The specimens were immersed into each liquid digestive medicine for 1, 3, 5, 10, 15, and 30 minutes and then evaluated each time by VHN. All digestive medicines had a pH of less than 3.7. Wicheongsu had the lowest pH 2.93 and Gashwalmyeungsu had highest pH 3.63. In pH 5.5, titratable acidity of Wicheongsu was 1.27 ml. Gashwalmyeungsu was 0.63 ml. Saengrokchun was 0.60 ml. All liquid digestive medicines showed low concentration of calcium and phosphate. The microhardness of specimens after immersion into liquid digestive medicines was continuously reduced in all digestive medicines. After 30-minute treatment in liquid digestive medicines, Wicheongsu containing low pH and high tiratable acidity was shown to be lowest microhardness value (207.80µ15.52). The three liquid digestive medicines caused surface softening of enamel erosion. We conclude that drinks, commonly consumed by functional dyspepsia patient can cause erosion of enamel.

Effect of bamboo salt on the caries activity of Streptococcus mutans / 대한구강보건학회지

OBJECTIVES: The purpose of this study was to evaluate the anti-caries activity of bamboo salt on Streptococcus mutans. METHODS: The pH of bamboo salt, bay salt, and NaCl were measured at concentrations of 1%, 3%, 5%, and 10%. At 3, 6, 9, 12, 18, and 24 h, the growth and acid production of S. mutans was measured using a spectrophotometer and pH meter, respectively. RESULTS: The growth of S. mutans remarkably reduced with the addition of 1% NaCl. Bamboo salt showed effective growth inhibition at concentrations higher than 3%. Bay salt showed effective growth inhibition at concentrations higher than 5% (P<0.01). At salt concentrations of 1%, the pH value of bacterial culture broth reduced to below 5.5 after 12 h. Bamboo salt and NaCl reduced acid production at concentrations higher than 3%. Moreover, bay salt decreased acid production at concentrations higher than 5%. CONCLUSIONS: These results showed that the bamboo salt inhibited growth and acid production of S. mutans. Thus, bamboo salt can be considered a useful material for the prevention of dental caries.

Risk factors of periodontal disease in Korean adults / 대한구강보건학회지

OBJECTIVES: The purpose of this study was to examine the risk factors for periodontal diseases in Korean adults. METHODS: In this study, raw data obtained from the third year of the 5th National Health and Nutrition Examination Survey (2010-2012) were used. The data were collected from 5,513 Korean adults, aged > or =19 years. Multiple logistic regression analysis was performed to determine the odds ratio (OR) and confidence interval in order to ascertain the factors influencing periodontal diseases. RESULTS: Multiple logistic regression analysis was performed to verify the risk factors responsible for periodontal diseases in Korean adults in 2012. After all factors were adjusted for, the following were identified as the only risk factors for periodontal diseases: sex, age, income level, educational level, occupation, history of smoking and alcohol consumption, usual stress recognition level, diabetes, self-assessed oral health, and daily tooth brushing frequency. CONCLUSIONS: The results of this study revealed that sociodemographic characteristics, health behavior and status, self-assessed oral health, and oral health were the factors that affect periodontal diseases.

Effect of bamboo salt on human gingival fibroblasts / 대한구강보건학회지

OBJECTIVES: To evaluate the anti-inflammatory and cytotoxic activities of bamboo salt. METHODS: Cytotoxicity of bamboo salt and bay salt (0.01%, 0.1%, and 1%) was evaluated using MTT assay. In addition, secretion of the pro-inflammatory cytokines interleukin (IL)-1beta and IL-6 from human gingival fibroblasts (HGFs) was measured after application of 0.01% and 0.1% concentrations by using real-time polymerase chain reaction. RESULTS: Bamboo salt and bay salt at 1% concentration were cytotoxic to HGFs at 24 h; however, no such effect was observed at 0.01% or 0.1%. Bamboo salt showed a relatively low inhibitory effect. IL-1beta secretion was inhibited by a 0.1% solution of bamboo salt. IL-6 secretion was inhibited by both bamboo salt and bay salt at 0.1% concentration. CONCLUSIONS: The above results suggest that bamboo salt inhibits the release of IL-1beta and IL-6 from HGFs. Thus, bamboo salt may be a useful material for gingival inflammation.

Effect of hangover beverage containing fluoride and calcium on enamel erosion / 대한구강보건학회지

OBJECTIVES: The purpose of this study was to evaluate the dental erosion inhibitory effect of hangover beverage containing calcium and fluoride. METHODS: Risk factors of dental erosion in the varying concentrations of fluoride, Ca, P, pH, and the buffer capacity were measured in six groups of mixture: distilled water, Morning care, Morning care adding 3% calcium, Morning care adding 5% calcium, Morning care adding 4 ppm F, Morning care adding 3% calcium and 4 ppm F. Seventy two specimens were prepared for the microhardness tests and divided randomly into 6 groups (n=12). Each group was exposed to the six groups of the mixture for 1, 3, 5, 10, 15, 30 min. Surface microhardness was measured before and after the treatment, and the surface was observed by SEM after the treatment only. RESULTS: After 30 minutes of treatment, the surface microhardness changes were significantly different among the six groups: Distilled water (0.11+/-1.98 DeltaVHN), Morning care (100.49+/-9.66 DeltaVHN), Morning care+3% calcium (17.07+/-8.45 DeltaVHN), Morning care+5% calcium (10.35+/-7.61 DeltaVHN), Morning care+4 ppm F (93.96+/-15.13 DeltaVHN), Morning care+3% calcium+4 ppm F (14.21+/-7.97 DeltaVHN) (P0.05) when compared with the distilled water group. CONCLUSIONS: It is suggested that modification of the Morning care with 3% calcium could be useful for a significant protective potential with respect to dental erosion.

Evaluation of the remineralization effect of bamboo salt and sodium fluoride solutions / 대한구강보건학회지

OBJECTIVES: The purpose of this investigation was to evaluate the remineralization effect of CPP-ACP, NaF, bamboo salt and NaF+bamboo salt solutions on bovine incipient artificial enamel caries by microcomputed tomography (micro CT). METHODS: Specimens were prepared from extracted bovine teeth and immersed in a demineralizing solution for 72 h. Specimens were divided into five groups of five specimens each by randomized blocks according to surface microhardness (VHN) and mineral density. The specimens were each treated by one of the following agents for 24 h: distilled water, 2% sodium fluoride solution (2% NaF group), 3% bamboo salt solution (3% BS group), and a solution of 2% sodium fluoride solution and 3% bamboo salt solution mixed together (2% NaF+3% BS group) and 1% CPP-ACP solution. After this, the specimens were immersed in artificial saliva for 15 and 30 days. After 15 days and 30 days treatment, VHN and mineral density values (g/cm3) were measured and analysed. Mineral density values were obtained using micro CT. RESULTS: Mineral density and VHN of the five groups according to the immersion period in artificial saliva showed statistically significant differences (P<0.05). In mineral density and VHN, the values of the 2% NaF+3% BS group were significantly higher than those of the other groups, and there were no significant differences among the 2% NaF, 3% BS, CPP-ACP groups after 30 days. CONCLUSIONS: Bamboo salt showed a similar remineralization pattern to CPP-ACP on an incipient artificial enamel caries lesion. Thus, bamboo salt (like CPP-ACP) can act as a remineralization agent for subsurface lesions of incipient enamel caries.