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Object To study the effects of the different Qinglongyi extracts (QE) on cell membrane fluidity of S 180 and H 22 tumor-bearing mice. Methods The tumor models of S 180 and H 22 were used. The oxter of the normal mice were injected S 180 tumor cell liquid and the abdomen of those were injected H 22 tumor cell liquid. Tumor-bearing mice were given QE by ip cold alcohol extract (191.37, 95.68, 47.84 mg/kg), hot alcohol extract (121.17, 60.58, 30.29 mg/kg), and cytoxan (20 mg/kg as positive control), normal saline (as negative control) for 7 d then the mice were killed, and the tumor cell membrane liquids were made according to the methods. The membrane fluidity was studied with fluorescence polarization (P) and microviscosity (?) with 1,6-dipheyl-1,3,5-hexatriene (DPH) as a probe. Results The QE by both cold and hot alcohol can make the membrane fluidity of S 180 tumor cell improve, the membrane fluidity of H 22 tumor cell decline. Compared with the normal saline control group, QE by both cold and hot alcohol can make the membrane fluidity of RBC (red blood cell) decline (P
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Object To observe antitumor effects of different extract fractions from Qinglongyi (DEQ). Methods S180-mice and H22-mice were given by ip as model. Antitumor effects of DEQ on S180-mice were observed, and also the effect on indexes of thymus and spleen, sialic acid (SA) content in cancer cell membrane and erythrocyte membrane in S180-mice and H22-mice. Results The two extract fractions by cold and hot alcohol have obvious antitumor effects in a dose-effect manner to S180-mice, and can prolong H22-mice life. The two extract fractions from Qinlongyi can significantly decrease SA in cancer cell membrane and increase SA in erythrocyte membrane. Conclusion The two extract fractions from Qinglongyi have antitumor effects.