Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Añadir filtros








Intervalo de año
1.
National Journal of Andrology ; (12): 428-434, 2006.
Artículo en Chino | WPRIM | ID: wpr-343605

RESUMEN

<p><b>OBJECTIVE</b>To evaluate the correlation of epididymal protease inhibitor(Eppin) and Semenogelin(Sg) on human ejaculated spermatozoa.</p><p><b>METHODS</b>The experimental approaches include: (1) Immunoprecipitation of Eppin with anti-Eppin from semen; (2) Colocalization of Eppin and Sg by immunofluorescence; (3) Immunoprecipitation of rEppin and rSg;(4) Far-Western blotting of rEppin and rSg;(5) Competition of saturated 125I-rSg binding to rEppin with unlabeled Sg, and direct binding of 125I-rSg to rEppin on a blot; (6) Autoradiography of 125I-rSg with rEppin.</p><p><b>RESULTS</b>Eppin-Sg complex present on the surface of human ejaculated spermatozoa, Cys-239 is the only cystein for rEppin binding rSg. Reduction and carboxymethylation of Cys-239 blocks binding of 125I-rEppin to rSg.</p><p><b>CONCLUSION</b>Our study demonstrates that Eppin and Sg bind to each other on human ejaculated spermatozoa. A disulfide linkage occurs between Sg and Eppin, indicating the specificity of binding.</p>


Asunto(s)
Humanos , Masculino , Unión Proteica , Proteínas Inhibidoras de Proteinasas Secretoras , Proteínas , Química , Metabolismo , Proteínas Recombinantes , Proteínas de Secreción de la Vesícula Seminal , Química , Metabolismo , Espermatozoides , Metabolismo
2.
Journal of Experimental Hematology ; (6): 224-227, 2004.
Artículo en Chino | WPRIM | ID: wpr-352093

RESUMEN

The aim of this study was to explore application value of detecting platelet associated antibody and platelet membrane glycoprotein in the diagnosis and prognosis for immune thrombocytopenia. The platelet associated immunoglobulin (PAIg) and platelet membrane glycoprotein (CD41, CD61, GPIIb/IIIa) in 76 cases of immune thrombocytopenia and 30 healthy subjects were determined by FCM. The results showed that PAIg level in ITP patients included PAIgG (31.25 +/- 18.06)%, PAIgM (32.41 +/- 15.51)%, PAIgA (23.39 +/- 16.67)% which were remarkedly higher than in health control (10.48 +/- 5.05)%, (9.40 +/- 4.42)% and (7.23 +/- 3.61)% (P < 0.001). In patients with secondary immune thrombocytopenia (chronic aplastic anemia, SLE, Evans syndrome, liver cirrhosis hypersplenism, etc), PAIg level was higher than that in control group, while the platelet membrane glycoprotein in the blood of these patients was lower than that in control group. The level of PAIg decreased (P < 0.05) after treatment, but platelet membrane glycoprotein increased (P < 0.01). The result suggested that measurements for platelet membrane glycoprotein and platelet associated antibody by FCM were practical with high sensitivity, rapidity and simplicity used as a routine method in diagnosis and evaluation of the therapeutic effects in immune thrombocytopenia patients.


Asunto(s)
Adolescente , Adulto , Anciano , Niño , Femenino , Humanos , Masculino , Persona de Mediana Edad , Plaquetas , Alergia e Inmunología , Citometría de Flujo , Inmunoglobulinas , Sangre , Integrina beta3 , Complejo GPIIb-IIIa de Glicoproteína Plaquetaria , Glicoproteína IIb de Membrana Plaquetaria , Glicoproteínas de Membrana Plaquetaria , Púrpura Trombocitopénica Idiopática , Sangre , Diagnóstico , Trombocitopenia , Sangre , Diagnóstico
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA