Effect of DEK targeting silence on proliferation and cell cycle of human hepatoma HepG2 cells / 新乡医学院学报

Objective To study the influence of targeted silencing of DEK on the proliferation and cell cycle of human hepatoma cell lines.Methods The human hepatoma cells line HepG2 were routinely cuhured and the cells were divided into blank control group,siRNA control group and DEK siRNA group when the cells grew to 90％ tusion.The cells in blank control group were cultured normally without any treatment;the cells in siRNA control group and DEK siRNA group were transfected with siRNA expression vector and DEK siRNA expression vector mediated by LipofectamineTM2000 liposomes,respectively.The expression of DEK mRNA in HepG2 cells was detected by real-time polymerase chain reaction;the expression of DEK and CyclinD1 protein in HepG2 cells was detected by Western blot;the proliferation of HepG2 cells was detected by methyl thiazolyl tetrazolium method,and the cell cycle was observed by flow cytometry.Results The expression of DEK mRNA in the blank control group,siRNA control group and DEK siRNA group was 0.826 ±0.052,0.776 ±0.051 and 0.420 ±0.050 respectively;the expression of DEK protein in the blank control group,siRNA control group and DEK siRNA group was 0.691 ± 0.073,0.726±0.061 and 0.311 ±0.038 respectively;the expression of CyclinDl protein in the blank control group,siRNA cuntrol group and DEK siRNA group was 0.712 ± 0.069,0.780 ± 0.074 and 0.434 ± 0.039 respectively.The expressions of DEK mRNA,DEK protein and CyclinD1 protein in DEK siRNA group were significantly lower than those in the blank control group and siRNA control group (P ＜ 0.05);there was no statistic difference in the expression of DEK mRNA,DEK protein and CyclinD1 protein between the blank control group and siRNA control group(P ＜0.05).The proliferation ability of HepG2 cells in DEK siRNA group after transfection of 24,48,72,96,120 h was significantly lower than that in the blank control group and siRNA control group(P ＜0.05);there was no statistic difference in the proliferation ability of HepG2 cells between the blank control group and siRNA control group at each time point(P ＜ 0.05).The proportion of G0 + G1 phase cells in DEK siRNA group was significantly higher than that in the blank control group and siRNA control group(P ＜ 0.05);the proportions of S phase and G2 + M phase cells in DEK siRNA group were significantly lower than those in the blank control group and siRNA control group(P ＜ 0.05);there was no statistic difference in the proportion of G0 + G1 phase,S phase and G2 + M phase cells between the blank control group and siRNA control group (P ＜ 0.05).The result of Pearson correlation analysis showed that the expression of CyclinD1 protein was positively correlated with the expression of DEK mRNA and protein(r =0.909,0.899;P ＜ 0.05).Conclusion DEK siRNA can inhibit the proliferation of HepG2 cells,and change the cell cycle distribution through down regulating the expression of DEK gene in HepG2 cells.This process may be related to the down regulation of the expression of CyclinD1.

Design of CT-guided puncture positioning device / 医疗卫生装备

Objective To design a CT-guided puncture positioning device to shorten puncture time while increase the accuracy. Methods The device realized 3D positioning by the principle of plane geometry, which was composed of a positioner,a fixation mechanism and a direction guiding mechanism for puncture needle.The positioner and direction guiding mechanism were made of transparent plastic, and the fixation mechanism was made of elastic cloth. Results The device gained advantages over the traditional means in puncture time,times of needle insertion and times of CT scanning,and had the puncture success rate in one time being 90%. Conclusion The device behaves well in focal positioning and puncture needle guiding,and thus decreases the probability of failed puncture and the patient pains.

Effect of Qingyi Chengqi Decoction on severe acute pancreatitis patients: a clinical study / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To observe the change of intra-abdominal pressure (IAP) in severe acute pancreatitis (SAP) patients, and to study the effect of Qingyi Chengqi Decoction (QCD) on it.</p><p><b>METHODS</b>Eighty-six SAP patients from Department of General Surgery and Department of Digestive Diseases, Qingyang People's Hospital, Gansu Province, who were in line with diagnosis standard of SAP, were assigned to the treatment group (44 cases) and the control group (42 cases) from March 2012 to May 2013. All patients received routine Western medicine. Those in the treatment group took QCD additionally. Main clinical symptoms and APACHE II were observed. The serum levels of amylase (AMY), C-reactive protein (CRP), and IAP were examined. The incidence of secondary infection rate (SIR), drainage rate (percutaneous catheter drainage and operation), mortality, and mean days in ward were also recorded.</p><p><b>RESULTS</b>Main clinical symptoms were significantly improved in the treatment group. APACHE II score, serum levels of AMY, CRP, and IAP obviously decreased in the treatment group. The incidence of SIR, drainage rate, and the mortality were also significantly lower in the treatment group than in the control group. The mean days in ward were also markedly shortened (P < 0.01).</p><p><b>CONCLUSION</b>QCD could relieve inflammatory response, lower IAP, SIR, and mortality, increase the curative rate and improve the prognosis of SAP.</p>

A study on the bonding properties of four resin cements / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To evaluate the dentin and enamel bonding strength and resin-dentin interfaces of four resin cements.</p><p><b>METHODS</b>Twenty-four sound freshly extracted molars were sectioned with low-speed saw under running water. Two mesial-distal enamel disc and two buccal-lingual dentin discs were sectioned from each molar. Four resin cements (A group: Panavia(TM) F; B group: RelyX(TM) ARC; C group: RelyX(TM) Unicem; D group: Clearfil(TM) SA Cement) were applied to the surface of these enamel and dentin discs according to manufacturers' guidance. The bonded specimen were prepared for shear bond strength test and sectioned occluso-gingivally into two slabs for scanning electron microscopy (SEM) observation.</p><p><b>RESULTS</b>The bonding strength between resin and enamel in B group [(29.38 ± 6.28) MPa] was higher than that in D group [(27.25 ± 7.03) MPa], and both of them were significantly higher than those in A [(22.92 ± 7.13) MPa] and C group [(10.92 ± 3.41) MPa] (P < 0.05). The highest dentin bonding strength was shown in B group [(27.28 ± 6.79) MPa], followed by A [(14.23 ± 6.39) MPa], D [(10.09 ± 3.26) MPa] and C group [(6.43 ± 1.60) MPa, P < 0.05].</p><p><b>CONCLUSIONS</b>The four resin cements have different enamel or dentin bonding properties.</p>

Discussion on five methods used for the determination of temporal clustering on infectious diseases / 中华流行病学杂志

To demonstrate and evaluate five different methods in the determination of temporal clustering on infectious diseases. The incidence rates of bacillary dysentery in Jinshui district, Zhengzhou city, Henan province from 2008 to 2010 were analyzed by 5 different methods-Cluster Analysis, Runs Test, Negative Binomial Distribution, Circular Distribution and Concentration Ratio. Through Cluster Analysis, data showed that the epidemic period was from May to Sept. with August as the peak. Runs Test confirmed a cluster of month-incidence in 2008 and 2009 (P < 0.05) and a random distribution in 2010. The Concentration Ratio showed a weakened seasonal incidence cluster to a certain extent by M from 2008 to 2010. The Circular Distribution demonstrated an inclining cluster of time (P < 0.01) and it was on July 11(th) and 29(th), as well on August 24(th) in 2008, 2009 and 2010. In terms of day-incidence, the Negative Binomial Distribution presented a cluster in 2008 and 2010, but with no significant difference in 2009. The five above said methods could flexibly be used in determining the temporal clustering of infectious disease at different occasions.

The spatial distribution and dynamics of human rabies cases at the county level in Henan province / 中华流行病学杂志

Objective To study the spatial distribution and dynamics of human rabies cases at the county level, in Henan province to provide scientific evidence for the development of control program on rabies. Methods Data of human rabies cases at the county level from 2004 to 2010 in Henan province were analyzed by Poisson distribution and negative binomial distribution. Data calculation was conducted manually. Results According to the level of α =0.05 being set, there were three different results appeared: the first was fitted negative binomial distribution in 2004, 2005,2007 and 2009; the second was prioritized negative binomial distribution, but the poisson distribution could not be excluded in 2008 and 2010; the last one was fitted neither negative binomial distribution nor poisson distribution in 2006. By the clustering parameter k, the clustering degree at county level decreased from 2004 to 2008, then ascending in 2009 but descending again in 2010. The degree of clustering showed a positive correlation with the county mean cases in the prevalent counties (r=0.807,P=0.028). Conclusion As a whole,the distribution of human rabies at county level in Henan from 2004 to 2010 showed negative binomial distribution and presented the spatial clustering.However, the degree of clustering decreased in recent years and showed that the infection resource was possibly scattered more evenly at the county level.

Effects of Swan-like Shape Memory Connector on stress shielding rate and callus development during experimental fracture healing process / 中国骨伤

<p><b>OBJECTIVE</b>To investigate the stress shielding rate, ultrastructural and biomechanical property of the healing bone fixed by Swan-like Shape Memory Connector (SMC) during experimental fracture healing in rabbits.</p><p><b>METHODS</b>Unilateral osteotomy of left humeral diaphysis was performed in 140 adult New Zealand rabbits. The humeral fracture was fixed with SMC in study group and 4-hole dynamic compression plate (DCP) in control group. The animals were sacrificed at the 2nd, 4th, 8th, 12th, and 16th weeks postoperatively. The humeral shafts were harvested for three-point bending tests and stress shielding rate examination. Samples from the fracture gaps were observed with scanning electron microscope.</p><p><b>RESULTS</b>Within an observation period of 12 weeks after operation, the stress shielding rates were significantly lower in study group than those in control group, and the bending strength and the bending rigidity were statistically higher. It was also found that collagen fibers in callus was arranged more regulate and early mineral deposition in study group.</p><p><b>CONCLUSION</b>These results suggest that SMC contributes to the proliferation and differentiation of osteoblasts, promoting the formation and calcification of bone matrix and enhancing biomechanical property of the healing bone, which might be closely correlated with a less stress shielding effect produced by SMC.</p>

Analyses on the isolation and characterization of canine rabies virus isolates in Henan / 中华流行病学杂志

<p><b>OBJECTIVE</b>To analyze the genetic characteristics of two rabies virus isolates from Henan province and to compare their relations hip with known rabies virus isolates and vaccine strains.</p><p><b>METHODS</b>Rabies viral antigens were detected in 100 canine brains by immunofluorescence assay method. Rabies virus was isolated through inoculating the suspensions of positive brains into suckling mice. N gene and G were amplified by RT-PCR and sequenced. Phylogenetic trees were constructed for the analysis on genetic characteristics of rabies virus.</p><p><b>RESULTS</b>Two rabies virus strains were isolated (Henan Hb1 and Henan Sq1). Data from sequential comparison revealed that the nucleotide and amino acid identities of N and G gene between the two isolates were 99.3% and 98.9%, and 98.7% and 98.4% respectively. The two isolates were more closely related to CTN, with the homogeny of N gene and G gene as 89.1% and 85.6%-85.7% at the nucleotide level, but 97.6%-98.0% and 92.3% at the amino acid level respectively, than to other vaccine strains. When comparing with other known viruses including Chinese isolates, the two stains shared closer identity with the isolates from Indonesia, and the rates of homogeny of N and G gene were 92.1%-93.2% and 91.9%-92.1% at the nucleotide level, 97.5%-98.6% and 96.0%-96.2% at the amino acid level, respectively. Data from the deduced amino acid sequences revealed that some amino acid residues including the residues in the N and G antigenic sites were substituted in the two isolates. Furthermore, phylogenetic analysis showed that the two isolates were also more closely related to the strains from Indonesia and vaccine strain CTN than to any other known street viruses and vaccine strains.</p><p><b>CONCLUSION</b>Both Henan Hbl and Henan Sql belonged to genotype 1. However, the N and G gene diverged from known street viruses and vaccine strains at either nucleotide level or amino acid level.</p>

Genetics subtypes and distribution of Seoul virus in Henan / 中华流行病学杂志

<p><b>OBJECTIVE</b>To investigate the sub-genotypes and distribution ot Seoul virus in Henan.</p><p><b>METHODS</b>Rodents were collected in the major epidemic areas and rats lungs were studied by indirect immunofluorescence assay. Partial M and S segments were amplified with nested reverse transcription-polymerase chain reaction using Hantavirus genotype-specific primers, sequenced, analyzed and compared with other known sequences.</p><p><b>RESULTS</b>The Hantavirus carried by Rattus norvegicus, Rattus flavipectus and Mus musculus were all belonged to Seoul virus in the main epidemic areas of Henan. We constructed two phylogenetic tree based on the partial M and S segment sequences while phylogenetic analysis distinguished three genetic subtypes (S1, S2 and S3). S1 and S3 were found main subtypes in Henan.</p><p><b>CONCLUSION</b>The results indicated that the genetic subtypes of Hantavirus were complicated and widely distributed in Henan.</p>