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Objective:To observe the expression of IL-35 in chronic sinusitis with nasal polyps(CRSwNP),and to complement the pathogenesis.Methods:The clinical data and nasal polyp tissues of CRSwNP who were hospitalized from May 2016 to April 2017 were randomly selected.The subjective symptoms were scored by visual analogue scale (VAS),the nasal endoscopy score by Lanza-Kennedy,and the CT score by Lund-Mackay.The IL-35 mRNA were detected by Real-time PCR in nasal polyp tissues,while the IL-35, IL-17 and IL-10 were detected by ELISA.Results: A total of 51 patients of the clinical data and nasal polyp tissues were collected.Among them,30 males and 21 females had the mean age of (43.3 ± 15.1) years,the VAS score was (5.5 ± 1.7),the Endoscopy score was(6.4±2.0),the CT score was(11.7±3.8).Our results of Real-time PCR and ELISA analysis showed that IL-35 mRNA and protein expression was significantly decreased in CRSwNP compared with normal nasal mucosa (P<0.05).But IL-17 and IL-10 were significantly increased by ELISA in CRSwNP compared with normal nasal mucosa (P<0.05).Conclusion: These data suggest that IL-35 may play an important role in the development and progression of CRSwNP.
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<p><b>OBJECTIVE</b>To observe the effect of ginseng polysaccharide (GPS) on the proliferation and apoptosis of human nasopharyngeal cancer cells CNE-2, and discuss the possible mechanism.</p><p><b>METHOD</b>The effect of GPS on the growth of CNE-2 cells was observed by CCK8 assay. CNE-2 cells in the logarithmic phase were collected and processed respectively with different concentrations (0, 0. 1, 0. 2, 0. 3. 0. 4 g L-1) of GPS for 48 h. The flow cytometry was used to detect its induction effect on CNE-2 cell apoptosis. Hoechst-33258 cell staining and electron microscope were used to observe the morphological changes of cells. The beta-catenin mRNA expression was detected by Real-time PCR. The protein localizations and expressions of beta-catenin and TCF4 were tested by the immunofluorescence staining. The expressions of beta-catenin, Bcl-2 and Bax proteins were detected by Western blot.</p><p><b>RESULT</b>CCK8 assay results showed that GPS could remarkably inhibit the proliferation of CNE-2 cells, with dose-time dependence. IC50 of cells induced with GPS for 48 h was 0. 39 g L-1. After being processed with GPS with concentrations of 0.1, 0. 2, 0. 3, 0. 4 g L-1 for 48 h, the cell apoptosis rates of human nasopharyngeal cancer cells CNE-2 were (5. 69 +/- 0. 29)% , (10. 3 +/- 0. 63)% , (15. 4 +/- 0. 74 ) % and (35. 7 +/- 1. 86)% , respectively. Significant difference was observed compared with the control group (2. 08 +/- 0. 11) % (P <0. 05). The results of Hoechst-33258 staining showed the characteristics of cell apoptosis. Under the electron microscope, apoptosis bodies could be observed among CNE-2 cells induced with GPS with the concentration of 0. 4 g L -1 for 48 h. The results of Real-time PCR showed a significant reduction in beta-catenin mRNA expression. The results of laser confocal microscopy revealed notable decrease of beta-catenin and TCF4 expression in nucleus and transfer from nucleus to cell membranes in beta-catenin expression areas after being processed with GPS for 48 h. Western blot showed significant decrease in the expressions of beta-catenin and anti-apoptosis protein Bcl-2, with an increasing expression in apoptosis-promoting protein Bax (P <0. 05).</p><p><b>CONCLUSION</b>GPS could significantly inhibit the proliferation of CNE-2 cells and promote thier apoptosis. The obstruction of Wnt/beta-catenin signaling pathway may be an important mechanism for GPS to induce the apoptosis of human nasopharyngeal cancer cells CNE-2.</p>