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Objective To investigate the role of leucine aminopeptidase in biliary obstructive dis-ease, and to evaluate the value of leucine aminopeptidase and its combination with alkaline phosphatase (AKP), glutamine transferase and 5′-nucleotidase (5′-NT) in diagnosis and treatment of bile duct obstruc-tion. Methods A total of 181 cases were collected, who were diagnosed as healthy, asymptomatic HBV carriers and patients with hepatitis, biliary obstruction, liver cirrhosis and primary liver cancer samples at Xiangya Hospital of Central South University from October 2016 to March 2017. The leucine aminopeptidase (LAP), AKP, gamma glutamyl transpeptidase (GGT) and 5′-NT were detected with the corresponding kits, and analyzed with different statistical methods. Results The highest level of LAP was in patients with biliary obstruction, compared to other groups, and the differences were statistically significant (P<0. 05). In biliary obstruction group, LAP and AKP, GGT and 5′-NT were correlated ( r=0. 690, P<0. 01; r=0. 864, P<0. 01;r=0. 735, P<0. 01). Receiver operating characteristic (ROC), curve area (AUC) of LAP, 5′-NT, GGT and AKP in the diagnosis of biliary obstruction were 0. 945 and 0. 898, 0. 942 and 0. 916;the AUC of LAP combined with 5′-NT, GGT and AKP was 0. 966;and the AUC of LAP combined with 5′-NT was 0. 968. Conclusions LAP can be used as a preliminary index of differential diagnosis of biliary obstructive disease, and its diagnostic value could be improved when combined with 5-NT.
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OBJECTIVE@#To establish the 2-dimensional electrophoresis (2-DE) map in colonic mucosa in sub-healthy people with shapeless stool and healthy people, to identify the differential proteins by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS), and to provide theoretical basis for the pathogenesis of intestinal mucosa in sub-healthy people with shapeless stool.@*METHODS@#Two-DE was used to separate the total proteins from the intestinal mucosa in sub-healthy people (the sub-health group) with the shapeless stool and healthy volunteers (the control group). ImageMaster 2D Elite soft was applied to analyze the 2-DE images, and the differentially expressed protein spots between the 2 groups were identified by MALDI-TOF-MS, protein bank and information technique.@*RESULTS@#We analyzed the average maps and obtained 517 protein spots in the sub-healthy group and 535 protein spots in the control group. Between the sub-healthy group and the control group, the mean of 366 protein spots was matched, and the matching rate was 70.79%. Ten differential protein spots were screened by MALDI-TOF-MS, and 8 were identified. Five out of the 8 spots were significantly decreased, while 3 out of the 8 were significantly increased.@*CONCLUSION@#The proteomic expression in colonic mucosa of people with shapeless stool is significantly different from that of healthy people. Eight differential proteins such as aldehyde dehydrogenase 1A1 isoform 1, 3-hydroxy-3-methylglutaryl-coenzyme A synthase 2 (mitochondrial), γ-actin, annexin A5 possibly involve in the pathogenesis of sub-healthy people with shapeless stool.
Asunto(s)
Femenino , Humanos , Masculino , Actinas , Metabolismo , Aldehído Deshidrogenasa , Metabolismo , Familia de Aldehído Deshidrogenasa 1 , Anexina A5 , Metabolismo , Estudios de Casos y Controles , Colon , Metabolismo , Dispepsia , Metabolismo , Electroforesis en Gel Bidimensional , Hidroximetilglutaril-CoA Sintasa , Metabolismo , Mucosa Intestinal , Metabolismo , Proteínas , Genética , Metabolismo , Proteoma , Proteómica , Métodos , Retinal-DeshidrogenasaRESUMEN
OBJECTIVE To invesigate the prevalence of SHV-producing Enterobacteriaceae isolates in Hunan Province and identify the subtype of SHV encoding gene and the epidemiological aspect of SHV-producing isolates. METHODS Isolates were collected and identified as well as subjected to ESBLs detection.PCR and DNA sequencing were used to determine the genotype of SHV enzymes.The homology of SHV-producing strains were detected by RAPD. RESULTS Twenty-six of 171 Enterobacteriaceae isolates were confirmed to produce blaSHV genes,and 5 subtypes of SHV-type ?-lactamases were determined,including 9 strains of SHV-28,7 strains of SHV-12,7 strains of SHV-1,2 strains of SHV-11 and 1 strain of SHV-5.There were 6 RAPD types in 19 isolates of SHV-producing Klebsiella pneumoniae,5 RAPD types in 5 isolates of SHV-producing Enterobacter cloacae. CONCLUSIONS SHV-12 is the predominant genotype of SHV ESBLs producing Enterobacteriaceae in Hunan Province,and clone spread has played a certain role in SHV-type ?-lactamase producing K.pneumoniae.SHV ESBLs are not found in Escherichia coli.