RESUMEN
OBJECTIVES: The purpose of this study was to investigate the effects of low-level laser therapy (LLLT) with a diode gallium-aluminum-arsenide (Ga-Al-As) low-level laser device on the healing and attachment of titanium implants in bone. MATERIALS AND METHODS: Thirteen New Zealand white male rabbits weighing 3.0+/-0.5 kg were used for this study. Dental titanium implants (3.75 mm in diameter and 8.5 mm in length, US II RBM plus fixture; Osstem, Seoul, Korea) were implanted into both femurs of each rabbit. The rabbits were randomly divided into a LLLT group and a control group. The LLLT was initiated immediately after surgery and then repeated daily for 7 consecutive days in the LLLT group. Six weeks and 12 weeks after implantation, we evaluated and compared the osseointegration of the LLLT group and control group, using histomorphometric analysis, removal torque testing, and resonance frequency analysis (RFA). The results were statistically significant when the level of probability was 0.05 or less based on a non-parametric Mann-Whitney U-test. RESULTS: The implant survival rate was about 96%. Histologically and histomorphometrically, we observed that the titanium implants were more strongly attached in LLLT group than in control group. However, there was no significant difference between the LLLT group and control group in removal torque or RFA. CONCLUSION: Histologically, LLLT might promote cell-level osseointegration of titanium implants, but there was no statistically significant effects.
Asunto(s)
Humanos , Masculino , Conejos , Experimentación Animal , Densidad Ósea , Implantes Dentales , Fémur , Terapia por Luz de Baja Intensidad , Nueva Zelanda , Oseointegración , Seúl , Tasa de Supervivencia , Titanio , TorqueRESUMEN
Bilateral sagittal split ramus osteotomy is considered a standard technique in mandibular orthognathic surgeries to reduce unexpected bilateral stress in the temporomandibular joints. Unilateral sagittal split ramus osteotomy (USSO) was recently introduced to correct facial asymmetry caused by asymmetric mandibular prognathism and has shown favorable outcomes. If unilateral surgery could guarantee long-term postoperative stability as well as favorable results, operation time and the incidence of postoperative complications could be reduced compared to those in bilateral surgery. This report highlights three consecutive cases with long-term follow-up in which USSO was used to correct asymmetric mandibular prognathism. Long-term postoperative changes in the condylar contour and ramus and condylar head length were analyzed using routine radiography and computed tomography. In addition, prior USSO studies were reviewed to outline clear criteria for applying this technique. In conclusion, patients showing functional-type asymmetry with predicted unilateral mandibular movement of less than 7 mm can be considered suitable candidates for USSO-based correction of asymmetric mandibular prognathism with or without maxillary arch surgeries.
Asunto(s)
Humanos , Asimetría Facial , Estudios de Seguimiento , Cabeza , Incidencia , Cirugía Ortognática , Osteotomía Sagital de Rama Mandibular , Complicaciones Posoperatorias , Prognatismo , Radiografía , Articulación TemporomandibularRESUMEN
Asunto(s)
Animales , Humanos , Anestesia General , Azaperona , Durapatita , Osteoblastos , Osteogénesis , Polietilenos , Polipropilenos , Semillas , PorcinosRESUMEN
0.05). Amnesia during local injection was observed in eight patients (34.8%). Compared with the preoperative anxiety score, the intraoperative anxiety score was decreased.CONCLUSION: In this study, we found cardiovascular and respiratory stability in intravenous sedation using dexmedetomidine with pethidine, in plate and screw removal, after orthognathic surgery. Furthemore, intravenous sedation using dexmedetomidine with pethidine shows adequate analgesic and sedative effects.
Asunto(s)
Humanos , Amnesia , Ansiedad , Presión Arterial , Presión Sanguínea , Dexmedetomidina , Frecuencia Cardíaca , Hipnóticos y Sedantes , Meperidina , Cirugía Ortognática , OxígenoRESUMEN
Asunto(s)
Huesos , Carbocianinas , Técnicas de Cultivo de Célula , Técnicas de Cocultivo , Colágeno , Colagenasas , Combinación de Medicamentos , Durapatita , Células Endoteliales , Laminina , Lipoproteínas , Magnetismo , Imanes , Mandíbula , Células Madre Mesenquimatosas , Tercer Molar , Nylons , Osteoblastos , Osteogénesis , Percloratos , Periostio , ProteoglicanosRESUMEN
0.05).CONCLUSION: The angle between the Lo line and IP line (angle of the Lo-IP line) showed no statistically significant difference in both the control and asymmetry groups. Therefore, the Lo line could be used as a horizontal reference plane in CBCT generated PA cephalograms.
Asunto(s)
Humanos , Tomografía Computarizada de Haz Cónico , Asimetría FacialRESUMEN
INTRODUCTION: The first aim of this study was to isolate the dental tissue-derived stem cells from the dental follicle (DF), dental pulp (DP), and root apical papilla (RAP) of the extracted wisdom teeth. Second was to evaluate their characterization with the expressions of transcription factors and cell surface markers. Finally, their ability of the in vitro multi-lineage differentiations into osteogenic and adipogenic cells were compared, respectively. MATERIALS AND METHODS: Dental tissues, including dental follicle, dental pulp, and root apical papilla, were separated in the extracted wisdom teeth. These three dental tissues were cultured in Dulbecco's modified Eagle's medium (DMEM) with supplements, respectively. After passage 3, the homogeneous shaped dental tissue-derived cells were analyzed the expression of transcription factors (Oct-4, Nanog and Sox-2) and cell surface markers (CD44, CD90 and CD105) with reverse transcription polymerase chain reaction (RT-PCR) and fluorescence-activated cell sorting (FACS) analysis. In order to evaluate in vitro multi-lineage differentiations, the culture media were changed to the osteogenic and adipogenic induction mediums when the dental tissue-derived cells reached to passage 3. The characteristics of these three dental tissue-derived cells were compared with immunohistochemistry. RESULTS: During primary culture, heterogenous and colony formatted dental tissue-derived cells were observed in the culture plates. After passage 2 or 3, homogenous spindle-like cells were observed in all culture plates. Transcription factors and mesenchymal stem cell markers were positively observed in all three types of dental tissue-derived cells. However, the quantity of expressed transcription factors was most large in RAP-derived cells. In all three types of dental tissue-derived cells, osteogenic and adipogenic differentiations were observed after treatment of specific induction media. In vitro adipogenic differentiation was similar among these three types of cells. In vitro osteogenic differentiation was most strongly and frequently observed in the RAP-derived cells, whereas rarely osteogenic differentiation was observed in the DP-derived cells. CONCLUSION: These findings suggest that three types of human dental tissue-derived cells from extracted wisdom teeth were multipotent mesenchymal stem cells, have the properties of multi-lineage differentiations. Especially, stem cells from root apical papilla (SCAP) have much advantage in osteogenic differentiation, whereas dental follicle cells (DFCs) have a characteristic of easy adipogenic differentiation.
Asunto(s)
Humanos , Medios de Cultivo , Pulpa Dental , Saco Dental , Durapatita , Citometría de Flujo , Imidazoles , Inmunohistoquímica , Células Madre Mesenquimatosas , Tercer Molar , Nitrocompuestos , Reacción en Cadena de la Polimerasa , Transcripción Reversa , Células Madre , Factores de TranscripciónRESUMEN
A melanotic neuroectodermal tumor of infancy (MNTI) is a uncommon osteolytic pigmented neoplasm that primarily affects the jaws of newborn infants. Most patients (> 90%) present with the tumor in the first year of life. Approximately 65% form in the maxilla, 11% in the mandible, 5% in the brain and elsewhere. MNTI is normally benign, but up to 15% may recur and a few have metastasized. Approximately 200 cases of MNTI have been reported but only 2 of them presented as multifocal. A case of MNTI in a 7 month old boy was encountered. The chief complaint was maxillary anterior ridge swelling. The incisional biopsy findings were MNTI. Two months after the first operation, mild swelling of another site was observed. The infant was examined periodically since undergoing two procedures with no recurrence. This case demonstrates the possibility of a multicentric MNTI. We report a multicentric MNTI with a review of the relevant literature.
Asunto(s)
Humanos , Lactante , Recién Nacido , Biopsia , Encéfalo , Maxilares , Mandíbula , Maxilar , Tumor Neuroectodérmico Melanótico , Polienos , RecurrenciaRESUMEN
Asunto(s)
Humanos , Colágeno , Combinación de Medicamentos , Durapatita , Células Endoteliales , Laminina , ProteoglicanosRESUMEN
Asunto(s)
Adulto , Humanos , Tejido Adiposo , Ácido Ascórbico , Desarrollo Óseo , Médula Ósea , Técnicas de Cultivo de Célula , Separación Celular , Colágeno , Colagenasas , Combinación de Medicamentos , Durapatita , Células Endoteliales , Factor de Crecimiento Epidérmico , Citometría de Flujo , Heparina , Hidrocortisona , Laminina , Magnetismo , Imanes , Células Madre Mesenquimatosas , Microesferas , Nylons , Osteoblastos , Osteotomía Sagital de Rama Mandibular , Prognatismo , Proteoglicanos , Piel , Células Madre , Factor A de Crecimiento Endotelial VascularRESUMEN
Asunto(s)
Humanos , Arterias , Cicatriz , Estética , Antebrazo , Colgajos Tisulares Libres , Mano , Fuerza de la Mano , Cabeza , Venas Yugulares , Maxilar , Boca , Suelo de la Boca , Mucosa Bucal , Cuello , Hueso Paladar , Nutrición Parenteral , Estudios Retrospectivos , Sensación , Piel , Cirugía Bucal , Tasa de Supervivencia , Muslo , Glándula Tiroides , Donantes de Tejidos , Lengua , Trasplantes , MuñecaRESUMEN
Asunto(s)
Humanos , Fosfatasa Alcalina , Ácido Ascórbico , Calcio , Técnicas de Cultivo de Célula , Proliferación Celular , Dexametasona , Durapatita , Mandíbula , Tercer Molar , Osteoblastos , Osteocalcina , Fenotipo , ARN Mensajero , EstroncioRESUMEN
Asunto(s)
Humanos , Asimetría Facial , Incisivo , Mandíbula , Osteotomía Sagital de Rama Mandibular , Prognatismo , Recurrencia , Estudios RetrospectivosRESUMEN
Asunto(s)
Femenino , Humanos , Mentón , Asimetría Facial , Huesos Faciales , Cabeza , Hipertrofia , Maloclusión , Cóndilo Mandibular , Mordida Abierta , Cirugía Ortognática , Osteocondroma , Músculos Pterigoideos , Esqueleto , Huelga de EmpleadosRESUMEN
INTRODUCTION: Skeletal homeostasis is normally maintained by the stability between bone formation by osteoblasts and bone resorption by osteoclasts. However, the correlation between the inflammatory reaction and osteoblastic differentiation of cultured osteoprogenitor cells has not been fully investigated. This study examined the effects of inflammatory cytokines on the osteoblastic differentiation of cultured human periosteal-derived cells. MATERIALS AND METHODS: Periosteal-derived cells were obtained from the mandibular periosteum and introduced into the cell culture. After passage 3, the periosteal-derived cells were further cultured in an osteogenic induction Dulbecco's modified Eagle's medium (DMEM) medium containing dexamethasone, ascorbic acid, and beta-glycerophosphate. In this culture medium, tumor necrosis factor (TNF)-alpha with different concentrations (0.1, 1, and 10 ng/mL) or interleukin (IL)-1beta with different concentrations (0.01, 0.1, and 1 ng/mL) were added. RESULTS: Both TNF-alpha and IL-1beta stimulated alkaline phosphatase (ALP) expression in the periosteal-derived cells. TNF-alpha and IL-1beta increased the level of ALP expression in a dose-dependent manner. Both TNF-alpha and IL-1beta also increased the level of alizarin red S staining in a dose-dependent manner during osteoblastic differentiation of cultured human periosteal-derived cells. CONCLUSION: These results suggest that inflammatory cytokines TNF-alpha and IL-1beta can stimulate the osteoblastic activity of cultured human periosteal-derived cells.
Asunto(s)
Humanos , Fosfatasa Alcalina , Antraquinonas , Ácido Ascórbico , Resorción Ósea , Técnicas de Cultivo de Célula , Citocinas , Dexametasona , Durapatita , Glicerofosfatos , Homeostasis , Interleucinas , Osteoblastos , Osteoclastos , Osteogénesis , Periostio , Factor de Necrosis Tumoral alfaRESUMEN
Asunto(s)
Adulto , Animales , Humanos , Regeneración Ósea , Trasplante Óseo , Durapatita , Oído , Fibrina , Adhesivo de Tejido de Fibrina , Minerales , Osteocalcina , Osteogénesis , Piel , Porcinos , TrasplantesRESUMEN
Asunto(s)
Humanos , Tomografía Computarizada de Haz Cónico , Cirugía Ortognática , Osteotomía Sagital de Rama Mandibular , Prognatismo , StentsRESUMEN
Asunto(s)
Adulto , Humanos , Diente Premolar , Labio Leporino , Constricción , Aglomeración , Estudios de Seguimiento , Hipogonadismo , Período de Latencia Psicosexual , Maloclusión , Máscaras , Maxilar , Enfermedades Mitocondriales , Oftalmoplejía , Aparatos Ortodóncicos , Ortodoncia , Osteogénesis por Distracción , Osteotomía , Hueso Paladar , Recurrencia , Diente , TracciónRESUMEN