RESUMEN
The study aimed to investigating the antinociceptive action of various fractions of methanolic extract of Terminalia Belliriccz roxb on Diabetic neuropathic rats. Diabetes was induced in over night fasted male adult Sprague Dawely rats by a single dose of intra peritoneal injection of streptozotocin 50mg/kg. Animals were divided into 8 groups, and treatment was started on 4[th] week with n-Butanol fraction [200 mg/ kg, p.o.], Ethyl acetate fraction [200 mg/ kg, p.o.], Water fraction [200 mg/ kg, p.o.], Fluoxetine [14.5 mg/kg, p.o.], Imipramine [10.5 mg/kg, p.o.] and Gallic acid[50 mg/kg, p.o.].Pain threshold has been evaluated in each week with analgesic models like Eddys hot plate method, Tail immersion method and Formaline test. Animals treated with Fluoxetine, Imipramine, Gallic Acid, n-butanol, Ethyl acetate and Water fractions has given significant result when compared with Diabetic control in each analgesic models. Among the fractions the result varies with the amount of Gallic acid present in it. Antinociceptive properties of various fractions of methanolic extract of Terminalia bellirica is due to the presence of Gallic acid and effect is through the restoration of brain monoamines, like nor epinephrine, 5-hydroxy tyrptamine [5-HT] and dopamine level
RESUMEN
The present study was aimed to investigate the effect of various fractions of methanolic extract of Terminalia bellirica on depression in rat using forced swim test [FST]. Antidepressant activity was evaluated by Forced swim test. Gallic acid [10, 30, 50, 100mg/kg], Fluoxetine, and Imipramine were administered in separate group of male Sprague rats one hour before the test. Gallic acid equivalent doses of various fractions of methanolic extract of Terminalia bellirica has been administered to a particular group of animals. The interference of antidepressant activity with gross motor activity of rats was determined by Open field test. Gallic acid shows a significant decrease in immobility time in dose dependent manner in FST. The efficacy of Gallic acid [50mg/kg] was found to be similar to that of Imipramine [10.5 mg/kg, p.o.] and Fluoxetine [14.5 mg/kg, p.o.]. Gallic acid equivalent dose of various fractions [200mg/kg] of methanolic extract of Terminalia bellirica showed a significant reduction in immobility time when compared to normal control and was not interfered with gross motor activity. Antidepressant property of various fractions of methanolic extract of Terminalia bellirica is due to the presence of Gallic acid. The antidepressant effect of Gallic acid may be through maintaining the level of nor adrenaline, 5-HT, and dopamine because TB extracts reversed reserpine induced depression suggested that antidepressant like effect of TB extracts may be through the restoration of brain monoamines, like nor epinephrine, 5-hydroxy tyrptamine [5-HT] and dopamine levels
RESUMEN
Objective: The present study was aimed to investigate the antioxidant activity of various fractions of methanolic extract of Terminalia bellirica roxb in Diabetic Neuropathic rats
Materials and Methods: Diabetes was induced by Streptozotocin [50mg/kg, i.p] Diabetic neuropathy was assessed by thermal and chemical stimuli. Antioxidant status has been evaluated. In this preventive therapy treatment has started with various fractions and gallic acid one day before the induction of diabetes and continued till 5[th] week of diabetes. Blood glucose level has been checked for each group and sciatic nerve histopathological studies have also done
Results: Animals treated with Gallic acid, n-butanol and ethyl acetate fractions of methanolic extract of Terminalia bellirica showed an extremely significant increase in the level of SOD, Catalase, reduced glutathione and decrease in the level of TEARS when compared to diabetic control and also significantly [p<0.001] increased the reaction time in Hot plate test, Hot water Tail immersion and significantly reduced the number of flinches in each phase in Formalin test when compared to diabetic control. Moreover animals treated with all these fractions except water fraction was not showed any significant effect in reaction time when compared to normal rat indicated the prevention of neuropathic pain. Diabetic animals treated with all fractions showed a gradual decrease in blood sugar in each week. Gallic acid and N-butanol shown preserved fiber density and vascular thickening was not observed when compared to diabetic control
Conclusion: Gallic acid present in the methanolic extract of Terminalia bellirica is the better option for diabetic neuropathy, that will act through two mechanisms i) by reducing the blood glucose level ii) due to the antioxidant activity they may retain the antioxidant status in animals to prevent the development of diabetic neuropathy