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Asian Journal of Andrology ; (6): 111-115, 2002.
Artículo en Inglés | WPRIM | ID: wpr-284062

RESUMEN

<p><b>AIM</b>To evaluate the occurrence and prevalence of microdeletions in the gamma chromosome of patients with azoospermia.</p><p><b>METHODS</b>DNA from 29 men with idiopathic azoospermia was screened by polymerase chain reaction (PCR) analysis with a set of gamma chromosome specific sequence-tagged sites (STSs) to determine microdeletions in the gamma chromosome.</p><p><b>RESULTS</b>Deletions in the DAZ (deleted in azoospermia) loci sgamma254 and sgamma255 were found in three patients with idiopathic azoospermia, resulting in an estimated frequency of deletions of 10.7% in idiopathic azoospermia men.</p><p><b>CONCLUSION</b>We conclude that PCR analysis is useful for the diagnosis of microdeletions in the Y chromosome, which is important when deciding the suitability of a patient for assisted reproductive technology such as testicular sperm extracion-intracytoplasmic sperm injection (TESE-ICSI).</p>


Asunto(s)
Adulto , Humanos , Masculino , Secuencia de Bases , Cromosomas Humanos Y , Cartilla de ADN , Eucromatina , Genética , Hormona Folículo Estimulante , Sangre , Heterocromatina , Genética , Hormona Luteinizante , Sangre , Oligospermia , Sangre , Genética , Reacción en Cadena de la Polimerasa , Prolactina , Sangre , Eliminación de Secuencia , Genética , Lugares Marcados de Secuencia , Testosterona , Sangre
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