Statistical optimization of culture conditions by response surface methodology for synthesis of lipase with Enterobacter aerogenes

Optimization of lipase production by Enterobacter aerogenes was carried out using response surface methodology (RSM) where the statistical model was obtained by fractional factorial central composite design. The influence of various physico-chemical parameters, viz. temperature, oil concentration, inoculum volume, pH and incubation period on lipase production was examined. Optimization of physico-chemical parameters resulted 1.4- fold increase in lipase activity. The optimum levels of parameters were 34°C, oil concentration 3 percent, inoculum volume 7 percent, pH 7 and incubation time 60 h for obtaining a maximum lipase activity of 27.25 U/ml.