Changes of CREB in rat hippocampus, prefrontal cortex and nucleus accumbens during three phases of morphine induced conditioned place preference in rats / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

<p><b>OBJECTIVE</b>To investigate the changes in CREB (cAMP response element binding protein) in hippocampus, PFC (prefrontal cortex) and NAc (nucleus accumbens) during three phases of morphine induced CPP (conditioned place preference) in rats, and to elucidate the role of CREB during the progress of conditioned place preference.</p><p><b>METHODS</b>Morphine induced CPP acquisition, extinction and drug primed reinstatement model was established, and CREB expression in each brain area was measured by Western Blot methods.</p><p><b>RESULTS</b>Eight alternating injections of morphine (10 mg/kg) induced CPP, and 8 d saline extinction training that extinguished CPP. CPP was reinstated following a priming injection of morphine (2.5 mg/kg). During the phases of CPP acquisition and reinstatement, the level of CREB expression was significantly changed in different brain areas.</p><p><b>CONCLUSION</b>It was proved that CPP model can be used as an effective tool to investigate the mechanisms underlying drug-induced reinstatement of drug seeking after extinction, and that morphine induced CPP and drug primed reinstatement may involve activation of the transcription factor CREB in several brain areas, suggesting that the CREB and its target gene regulation pathway may mediate the basic mechanism underlying opioid dependence and its drug seeking behavior.</p>

Establishment of computer-based video-tracking conditioned place preference experiment system in mice / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To establish the computer-based video-tracking conditioned place preference (CPP) system in mice.</p><p><b>METHODS</b>The CPP system was composed of computer, camera, soundproof box, shuttle box and analytical software. The results of morphine-induced conditioned place preference were used to evaluate the experiment system. And the effect of morphine-induced locomotor activity in drug-paired compartment was studied in mice.</p><p><b>RESULTS</b>Low (1 mg/kg, i.p.), moderate (3 mg/kg, 5 mg/kg, i.p.) and high (10 mg/kg, i.p.) dose of morphine significantly prolonged the time mice spent in drug-paired compartment compared with saline, but there was no dose-response relation. Moderate and high dose of morphine significantly enhanced locomotor activity, among which 5 mg/kg and 10 mg/kg morphine induced behavior sensitization in drug-paired compartment during the conditioning sessions.</p><p><b>CONCLUSION</b>The computer-based video-tracking conditioned place preference experiment system in mice established successfully is reliable and stable.</p>

Quantitative study by grey system on the latent period of lung cancer induced by air pollutants / 中华流行病学杂志

<p><b>OBJECTIVE</b>To calculate the latent period of lung cancer induced by air pollution.</p><p><b>METHODS</b>The degree of grey incidence (DGI) between the concentrations of TSP or SO(2) and the mortality of lung cancer were assessed through a grey system model. According to the maximum values of DGI, the total latent period of lung cancer was calculated. Data was collected in H city.</p><p><b>RESULTS</b>The maximum DGI value of TSP was 0.886 while the relationship between the comparison sequence from 1985 to 1989 and the reference sequence from 1994 to 1998 was greatly correlated. The maximum DGI value of SO(2) was 0.919 while the relationship between the comparison sequence from 1986 to 1990 and the reference sequence from 1994 to 1998 was most correlated.</p><p><b>CONCLUSIONS</b>The latent periods of lung cancer induced by TSP and SO(2) were 7 and 8 years respectively in H city. The model of grey system was less affected by the confounding factors, and the calculation process was relatively simple. A feasible and useful new way was provided by the model of grey system for quantitative analysis of the latent period of lung cancer induced by air pollutants.</p>